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ApplicationPostdoctoral Scientist
Josefin Soppert
Boxgraben 73, 52064 Aachen
9th November 1988, Single
+49 (0)163 5005314
Josefin Soppert
Dear Ladies and Gentlemen,
I want to pursue my career as researcher in a biotech company that focused on the research of pharma-ceutical drugs and new biological entities. I will use my skills and passion to contribute to the company‘s purpose discovering and delivering new ways to improve people’s health.
I am currently completing my PhD in molecular and applied biotechnology at the RWTH Aachen University with a strong cardiovascular background using biochemical, molecular and cell biological methods. I am going to graduate in July 2017 and can start working in August.
During my PhD project, I worked with primary cells to elucidate the effect of a cytokine and its soluble receptor on survival and downstream signaling events. I performed inhibition studies and explored the underlying mechanisms using cell-based assays such as viability/proliferation assays, protein kinase activation, flow cytometry, fluorescence microscopy and ELISA.
Additionally, I participated in a Felasa B and cardiovascular methods workshop where I learnt the handling of animals and to apply in vivo techniques in mice, respectively. To gain practice, I assisted during surgery as well as measured heart function before and after onset of infarction.
Regular meetings with colleagues and presentations at several conferences ensured a constant exchange of scientific knowledge and ideas. Motivation and strong desire to succeed encouraged the progress of my PhD project. I am used to independent work including the development of ideas, designing, planning and performing experiments and finally, statistical analysis and interpretation of data.
Coordination of several projects as well as mentoring of undergraduate and graduate students equipped me with organizational and prioritizing skills. As a proficient team player, I can collaborate with peers at all levels and utilize the expertise of colleagues as well as offering my own skills to work efficient and results-oriented.
I appreciate to meet you and to discuss how my qualifications and enthusiasm will be beneficial to your organization’ success.
Sincerely, Josefin Soppert
Applicationas postdoctoral scientist
Boxgraben 73, 52064 Aachen
9th November 1988, Single
+49 (0)163 5005314
Josefin Soppert
Working ExperienceSince October 2013
September 2012 - September 2013
April 2011 - September 2011
January 2011 - April 2011
February 2010 - July 2010
Research Associate, PhD Thesis, Focus: Cardiovascular Diseases Department of Thoracic and Cardiac Surgery, RWTH University Hospital, Aachen
Role of MIF family members in myocardial fibrosis and protection Using in vivo and in vitro models and biochemistry, molecular biological and cell-based methods
Writing and publishing papers and presenting at several conferences
Mentoring of graduate and undergraduate students and supervision of projects
Graduate Student, Master Thesis, Focus: Cardioprotection Institute of Biochemistry and Molecular Biology, RWTH Aachen
Role of MIF in ischemic-induced preconditioning Established preconditioning protocol for primary cardiac cells Presented frequently latest results in front of colleagues
Graduate Student, Internship, Focus: Starvation Massey University, New Zealand
Signal transduction pathway of amino acid starvation Performed in vitro binding assays using GST fusion proteins Improved protocol for efficient semi-dry transfer of proteins of diverse sizes
Undergraduate Student, Bachelor Thesis Focus: Protein Expression & Purification Beiersdorf AG in cooperation with Hamburg University of Applied Science
Generation of body odor precursors Expressed, purified and assessed activity of relevant proteins Optimized transfection protocol for insect cells
Undergraduate Student, Internship Focus: Automated Patch-Clamp Evotec AG, Department Electrophysiology
Performance evaluation of NanoPatch96-beta-test unit from Nanion Technologies, Revealed hardware and software issues Suggested improvements of the analytical program for a better operator convenience
Boxgraben 73, 52064 Aachen
9th November 1988, Single
+49 (0)163 5005314
Josefin Soppert
Academic Education
Semester Abroad
Since October 2013 - Expected July 2017
August 2010 - December 2010
October 2011 - September 2013
October 2007 - April 2011
August 1999 - July 2007
PhD in Molecular and Applied Biotechnology, RWTH Aachen University
Norwegian University of Life Sciences (UMB)
Master of Science in Molecular and Applied Biotechnology, RWTH Aachen University, Final Grade: 1.1
Bachelor of Science in Biotechnology, Hamburg University of Applied Science, Finale Grade: 1.8
Abitur at Geschwister-Scholl-Gymnasium Nossen, SachsenFinale Grade: 2.0
Workshops/QualificationsTraining and permission to carry out animal experiments (Felasa B)
Cardiovascular methods seminar LAD occlusion, Millar catheter, echocardiography
Awards
2014: Springorum Denkmünze 2014: Travelling award from WAKWIN
Boxgraben 73, 52064 Aachen
9th November 1988, Single
+49 (0)163 5005314
Josefin Soppert
Key SkillsInitiative, communicative, project managing, teamwork-oriented, Sense of responsibility, results-driven
Computational Skills Proficient in Microsoft Office Endnote and GraphPadPrism, Photoshop
Laboratory Skills
Mouse handling Blood collection, organ removal Anesthesia, surgery, suture, echocardiography
Cell culture techniques Cultivation of cell lines and isolation of primary cells
Molecular biological methods Cloning, ligation, plasmid isolation, transformation Bacmid-DNA purification, PCR, agarose gel electrophorese
Biochemical methods SDS-PAGE, Western blot, immunodetection Co-immunoprecipitation, ELISA, flow cytometry Immunofluorescence staining, immunohistochemistry
Cell biological methods Transfection, cell survival and enzymatic activity assays
Language
Fluent in English (spoken and written)
Interests and ActivitiesVolunteered as lifeguard at Baltic see Trained lifeguards Oil painting, fitness, running
Boxgraben 73, 52064 Aachen
9th November 1988, Single
+49 (0)163 5005314
Josefin Soppert
Publications
J. Soppert, S. Kraemer, C. Stoppe, F. Ulmer. Argon-preconditioning of liver cells.In preparation
J. Soppert, S. Kraemer, C. Beckers, A. Goetzenich, J. Bernhagen, C. Stoppe. Low sCD74/MIF ratio protects myofibroblasts against oxidative stress. In preparation
J. Soppert, S. Kraemer, C. Beckers, A. Goetzenich, J. Bernhagen, C. Stoppe. Antifibrotic effects of high sCD74/MIF ratio is partially mediated by necroptosis. In preparation
J. Soppert, B. Mayer, S. Kraemer, S. Schemmel, C. Beckers, C. Bleilevens, R. Rossaint, M. Coburn, A. Go-etzenich, C. Stoppe. Argon Induces Protective Effects in Cardiomyocytes during the Second Window of Preconditioning. Int J Mol Sci. 2016; 17(7).
C. Stoppe, L. Averdunk, A. Goetzenich, J. Soppert, A. Marlier, S. Kraemer, M. Coburn, A. Stevanovic, BS. Kim, S. Rex, A. Ochi, L. Leng, L. Cantley, G. Moeckel, G. Marx, J. Bernhagen, S. Djudjaj, R. Bucala, MD, P. Boor. The protective role of macrophage migration inhibitory factor (MIF) in acute kidney injury after cardiac surgery. Under review
B.S. Kim, D. Jacobs, C. Emontzpohl, A. Goetzenich, J. Soppert, M. Jarchow, L. Schindler, L. Averdunk, S. Kraemer, G. Marx, J. Bernhagen, N. Pallua, H.P. Schlemme, D. Simons, C. Stoppe. Myocardial Ischemia Induces SDF-1α Release in Cardiac Surgery Patients.J Cardiovasc Transl Res. 2016; 9(3):230-8.
D. Dreymueller, A. Goetzenich, C. Emontzpohl, J. Soppert, A. Ludwig, C. Stoppe. The perioperative time course and clinical significance of the chemokine CXCL16 in patients undergoing cardiac surgery. J Cell Mol Med. 2016; 20(1):104-15.
C. Stoppe, S. Rex, A. Goetzenich, S. Kraemer, C. Emontzpohl, J. Soppert, L. Averdunk, Y. Sun, R. Rossaint, H. Lue, C. Huang, Y. Song, G. Pantouris, E. Lolis, L. Leng, W. Schulte, R. Bucala, C. Weber, J. Bernhagen. Interaction of MIF Family Proteins in Myocardial Ischemia/Reperfusion Damage and Their Influence on Clinical Outcome of Cardiac Surgery Patients. Antioxid Redox Signal. 2015;23(11):865-79
S. Rex, S. Kraemer, G. Grieb, C. Emontzpohl, J. Soppert, A. Goetzenich, R. Rossaint, J. Bernhagen, C. Stoppe. The role of macrophage migration inhibitory factor in critical illness. Mini Rev Med Chem. 2014;14(14):1116-24. Review
A. Goetzenich, S. Kraemer, R Rossaint, C. Bleilevens, F. Dollo, L. Siry, S. Rajabi-Alampour, C. Beckers, J. Soppert, H. Lue, S. Rex, J. Bernhagen, C. Stoppe. The role of macrophage migration inhibitory factor in anesthetic-induced myocardial preconditioning. PLoS One. 2014; 9(3):e92827.
Boxgraben 73, 52064 Aachen
9th November 1988, Single
+49 (0)163 5005314
Josefin Soppert
References
Sandra [email protected]
Christian [email protected]
Jürgen [email protected]
Presentations/ePostersJ. Soppert; P. Meybohm; K. Hoffmann; S. Kraemer; A. Goetzenich; G. Marx; J. Bernhagen; C. Die funktionelle Rolle des pleiotropen Zytokins MIF innerhalb der ferninduzierten ischämischen Präkonditionierung in der Herzchirurgie. Deutscher Anästhesie Congress, 2016, ePoster
J. Soppert, S. Kraemer, J. Bernhagen, A. Goetzenich, C. Stoppe. Underlying mechanism of sCD74/MIF complex formation in myocar-dial protection. Deutsche Gesellschaft für Thorax und Herzchirurgie 2016. Poster
J. Soppert, S. Kraemer, A. Goetzenich, R. Rossaint, J. Bernhagen, C. Stoppe. Die Interaktion und funktionelle Bedeutung von MIF und seinem löslichen Rezeptor CD74 innerhalb der myokardialen Kardio-protektion. Deutscher Anästhesie Congress 2015. Presentation.
J. Soppert, S. Kraemer, J. Bernhagen, A. Goetzenich, C. Stoppe, R. Autschbach. The soluble form of CD74 enhances MIF’s cardiopro-tective properties. Gesellschaft für Thorax und Herzchirurgie 2015. Poster
J. Soppert, S. Kraemer, A. Goetzenich, R. Rossaint, J. Bernhagen, C. Stoppe. Die funktionelle Rolle des Makrophagen-migrationsinhibie-renden Faktors in der myokardialen Präkonditionierung. Deutscher Anästhesie Congress 2014. Presentation.
