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7/30/2019 Antrodia camphorata - Liver - Pubmed Paper
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1: J Agric Food Chem. 2005 Jul 13;53(14):5559-64.
http://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&Cmd=ShowDetailView&TermToSearch=1599
8114&ordinalpos=2&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSu
m
Mycelia from Antrodia camphorata in Submerged culture induce apoptosis of human hepatoma
HepG2 cells possibly through regulation of Fas pathway.
Song TY, Hsu SL, Yeh CT, Yen GC.
Department of Food Science, National Chung Hsing University, 250 Kuokuang Road, Taichung 40227,
Taiwan.
The objective of this study was to investigate the antiproliferative effect and the mechanism of the
methanol extracts of mycelia (MEM) form Antrodia camphorata in submerged culture toward HepG2
cells. The results showed that MEM-induced cell apoptosis involved up-regulation of Fas and
down-regulation of Bcl-2, DR3, DR4, TNFRI, and TNFRII in HepG2 cells, while no changes on the
levels of Bax, Bid, Bad, and Bak protein were observed. On the basis of these results, the involvement
of the Fas/Fas ligand (FasL) death-receptor pathway, in MEM-induced apoptosis in HepG2 cells, was
investigated. The apoptosis inducing activity was significantly enhanced by a Fas activator and
inhibited by a Fas antagonist. To know about the effect of MEM on the activation of the apoptotic
pathway, the adenovirus transfected with Bcl-2 was infected on HepG2 cells. The data showed that thepercentage of apoptotic cells induced by MEM in Bcl-2-infected HepG2 (Bcl-2 overexpression) was
not significantly different from that of uninfected HepG2. These results demonstrate that MEM induces
HepG2 apoptosis through inhibition of cell growth and up-regulation of Fas/FasL to activate the
pathway of caspase-3 and -8 cascades.
PMID: 15998114 [PubMed - indexed for MEDLINE]
2: J Ethnopharmacol. 2005 Aug 22;100(1-2):158-67.
Induction of apoptosis in human hepatoma cells by mycelia of Antrodia camphorata in
submerged culture.
Song TY, Hsu SL, Yen GC.Department of Food Science, National Chung Hsing University, 250 Kuokuang Road, Taichung 40227,
Taiwan.
The effect of methanolic extracts of mycelia (MEM) from Antrodia camphorata (Polyporaceac,
Aphyllophorales) of submerged culture (ACSC) on the inhibition of cell viability and the mechanism of
MEM-induced cytotoxic in hepatoma cells were investigated. The IC(50) of MEM on the cytotoxicity
of HepG2 (wild type p53) and Hep3B (delete p53) were 49.5 and 62.7 microg/ml, respectively, on 48 h
incubation. There is no observable cytotoxicity of MEM in Chang liver cells and rat primary
hepatocytes at the concentration of 100 microg/ml. Cell cycle analysis revealed that MEM induced
apoptosis on HepG2 via G0/G1 cell cycle arrest. MEM (100 microg/ml) treated HepG2 and Hep3B for72 h, the apoptotic cells were 98.3 and 39.5%, respectively. The activities of caspase-3, -8 and -9 in
HepG2 induced by MEM (50 microg/ml) were increased 5.3, 6.7 and 2.2-fold, respectively.
MEM-induced apoptotic cell death was accompanied by up-regulation of caspase-3 and -8 in HepG2
cells. Combined treatment with MEM and caspase-3, -8 and -9 inhibitors, the caspase-3 and -8
inhibitors were accounting for 63 and 47% inhibition in MEM-induced apoptosis, respectively;
however, caspase-9 inhibitor exhibited no obvious inhibition effect on the apoptosis percentage
(p>0.05). The results indicated that MEM induced HepG2 apoptosis through activation of caspase-3
and -8 cascades and regulation of the cell cycle progression to inhibit hepatoma cells proliferation.
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Publication Types: Research Support, Non-U.S. Gov't
PMID: 15949907 [PubMed - indexed for MEDLINE]
3: Cancer Lett. 2005 Apr 18;221(1):77-89.
Apoptotic effects of extract from Antrodia camphorata fruiting bodies in human hepatocellular
carcinoma cell lines.
Hsu YL, Kuo YC, Kuo PL, Ng LT, Kuo YH, Lin CC.
Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, No. 100
Shin-Chuan 1st Road, Kaohsiung 807, Taiwan, ROC.
The fruiting body of Antrodia camphorata is well known in Taiwan as a traditional medicine for
treating cancer and inflammation. The purpose of this study was to evaluate the apoptotic effects of
ethylacetate extract from A. camphorata (EAC) fruiting bodies in two human liver cancer cell lines,
Hep G2 and PLC/PRF/5. Treatment with EAC decreased the cell growth of Hep G2 and PLC/PRF/5
cells in a dose dependent manner. In Fas/APO-1 positive-Hep G2 cells, EAC increased the expression
level of Fas/APO-1 and its two forms of ligands, membrane-bound Fas ligand (mFasL) and soluble Fas
ligand (sFasL), in a p53-indenpendent manner. In addition, EAC also initiated mitochondrial apoptotic
pathway through regulation of Bcl-2 family proteins expression, release of cytochrome c, andactivation of caspase-9 both in Hep G2 and PLC/PRF/5 cells. Furthermore, EAC also inhibited the cell
survival signaling by enhancing the amount of IkappaBalpha in cytoplasm and reducing the level and
activity of NF-kappaB in the nucleus, and subsequently attenuated the expression of Bcl-X(L) in Hep
G2 and PLC/PRF/5 cells. EAC therefore decreased the cell growth and induced apoptosis both in Hep
G2 and PLC/PRF/5 cells.
Publication Types: Research Support, Non-U.S. Gov't
PMID: 15797630 [PubMed - indexed for MEDLINE]
4: Toxicol Appl Pharmacol. 2004 Dec 1;201(2):186-93.
Antitumor effects of the partially purified polysaccharides from Antrodia camphorata and themechanism of its action.
Liu JJ, Huang TS, Hsu ML, Chen CC, Lin WS, Lu FJ, Chang WH.
Graduate Institute of Biochemistry and Molecular Biology, College of Medicine, National Taiwan
University, Taipei, Taiwan.
Antrodia camphorata is a popular folk medicine that has attracted great attention due to its fame for
antitumor activity against cancer. However, there is little information available about its action. In the
present study, we purified a unique polysaccharide component from A. camphorata mycelia (AC-PS)
and found that it has pronounced anti-tumor effects on both in vitro and in vivo model. Our results
showed that AC-PS alone did not show any direct cytotoxic effect to human leukemic U937 cells, evenat high concentration (200 microg/ml). However, it could inhibit the proliferation of U937 cells via
activation of mononuclear cells (MNCs). Treatment of U937 cells with AC-PS-stimulated-MNC-CM
could significantly inhibit its proliferation with 55.3% growth inhibition rate. The in vitro antitumor
activity was substantiated by the in vivo therapeutical study of AC-PS in sarcoma 180-bearing mice.
Intraperitoneal and oral administration of AC-PS, 100 and 200 mg/kg significantly suppressed the
tumor growth with the inhibition rate of 69.1% and 58.8%, respectively. In vivo studies also showed
that several immunoparameters, such as the spontaneous proliferation of spleen cells, after AC-PS
administration, were two-fold higher than in control mice. Furthermore, the cytolytic activity of spleen
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cells also increased from 9.8 +/- 1.1% in control mice to 34.2 +/- 5.5% and 48.2 +/- 2.5%, after oral and
intraperitoneal treatment, respectively. Besides, the mice serum interleukin-12 levels increased
significantly by AC-PS treatment. Considering all these results, it is suggested that AC-PS elicit its
anti-tumor effect by promoting a Th1-dominant state and killer activities.
Publication Types: Research Support, Non-U.S. Gov't
PMID: 15541758 [PubMed - indexed for MEDLINE]
5: J Agric Food Chem. 2003 May 21;51(11):3302-8.
Antioxidative and hepatoprotective effects of Antrodia camphorata extract.
Hsiao G, Shen MY, Lin KH, Lan MH, Wu LY, Chou DS, Lin CH, Su CH, Sheu JR.
Department of Pharmacology and Graduate Institute of Medical Sciences, Taipei Medical University,
250 Wu-Hsing Street, Taipei 110, Taiwan.
Antrodia camphorata (A. camphorata) is well-known in Taiwan as a traditional Chinese medicine. The
purpose of this study was to evaluate the ability of A. camphorata extracts to protect against oxidative
stress in vitro and against carbon tetrachloride (CCl(4))-induced hepatic injury in vivo. An extract of A.
camphorata inhibited nonenzymatic iron-induced lipid peroxidation in rat brain homogenates with an
IC(50) value about 3.1 mg/mL. It also scavenged the stable free radical 1,1-diphenyl-2-picrylhydrazyl
(DPPH). The dose of the A. camphorata extract resulting in a decrease of 0.20 in the absorbance of
DPPH was about 31 +/- 0.7 microg/mL. Furthermore, an A. camphorata extract dose-dependently
(250-1250 mg/kg) ameliorated the increase in plasma aspartate aminotransferase (GOT) and alanine
aminotransferase (GPT) levels caused by chronic repeated CCl(4) intoxication in mice. Moreover, A.
camphorata extract significantly improved the CCl(4)-induced increase in hepatic glutathione
peroxidase, reductase, and CCl(4)-induced decrease in superoxide dismutase activities. It also restored
the decrement in the glutathione content and catalase activity of hepatic tissues in CCl(4)-intoxicated
mice. Furthermore, it also dose-dependently inhibited the formation of lipid peroxidative products
during CCl(4) treatment. Histopathological changes of hepatic lesions induced by CCl(4) were
significantly ameliorated by treatment with an A. camphorata extract in a dose-dependent manner.
These results suggest that A. camphorata extract exerts effective protection against chronicchemical-induced hepatic injury in vivo, by mediating antioxidative and free radical scavenging
activities.
Publication Types: Research Support, Non-U.S. Gov't
PMID: 12744658 [PubMed - indexed for MEDLINE]
6: J Agric Food Chem. 2003 Mar 12;51(6):1571-7.
Protective effects of fermented filtrate from Antrodia camphorata in submerged
culture against CCl4-induced hepatic toxicity in rats.
Song TY, Yen GC.
Department of Food Science, National Chung Hsing University, 250 Kuokuang Road, Taichung 40227,Taiwan, Republic of China.
The protective effects and the possible mechanisms of dry matter of fermented filtrate (DMF) from
Antrodia camphorata in submerged culture (ACSC) on H(2)O(2)-induced cytotoxicity in HepG2 and
carbon tetrachloride (CCl(4))-induced hepatotoxicity in Sprague-Dawley rats were investigated. The
results showed that the inhibitory effect of DMF and its crude triterpenoids on lipid peroxidation
occurred in a dose-response manner in an AAPH/linoleic acid system. When HepG2 cells were
pretreated with DMF at the concentration of 0.10 mg/mL for 4 h and then induced by 1 h of treatment
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with H(2)O(2) (100 microM), lipid peroxidation was significantly (p < 0.05) decreased, as measured by
the formation of malondialdehyde. The oral pretreatment with DMF [0.25 and 0.50 mg/kg of body
weight (bw)] for 5 consecutive days prior to the administration of a single dose of 40% CCl(4) (0.10
mL/100 g of bw, ip) significantly prevented the increase in serum levels of hepatic enzyme markers
(alanine and aspartate aminotransferase) and liver lipid peroxidation (p < 0.05). Histopathological
evaluation of the rat liver revealed that DMF reduced the incidence of liver lesions, including
neutrophil infiltration, hydropic swelling, and necrosis induced by CCl(4) in rats. Moreover, reduced
glutathione (GSH)-dependent enzymes (glutathione peroxidase, glutathione reductase, and glutathione
S-transferase) and the GSH/GSSG ratio were significantly improved in the oral pretreatment DMF of
rats (p < 0.01). The results suggest that DMF may play a role in preventing oxidative damage in living
systems by up-regulating hepatic GSH-dependent enzymes to preserve the normal GSH/GSSH ratio
and scavenging free radicals formed during CCl(4) metabolism.
Publication Types:Research Support, Non-U.S. Gov't
PMID: 12617586 [PubMed - indexed for MEDLINE]
7: Antrodia cinnamomea fruiting bodies extract suppresses the invasive potential of human liver
cancer cell line PLC/PRF/5 through inhibition of nuclear factor kappaB pathway.
Hsu YL, Kuo PL, Cho CY, Ni WC, Tzeng TF, Ng LT, Kuo YH, Lin CC.
Department of Pharmacy, Chia-Nan University of Pharmacy and Science, Tainan, Taiwan.
In this study, we first report the anti-invasive effect of ethylacetate extract from Antrodia cinnamomea
(EAC) fruiting bodies in the human liver cancer cell line PLC/PRF/5. Treatment with EAC decreased
the cancer invasion of PLC/PRF/5 cells in a dose-dependent manner. This effect was strongly
associated with a concomitant decrease in either the level or activity of VEGF, MMP-2, MMP-9 and
MT1-MMP, and an increase in the expression of TIMP-1 and TIMP-2. EAC inhibited constitutively
activated and inducible NF-kappaB in both its DNA-binding activity and transcriptional activity.
Furthermore, EAC also inhibited the TNF-alpha-activated NF-kappaB-dependent reporter geneexpression of MMP-9 and VEGF, and the invasion of cancer cells. EAC also exhibited an inhibitory
effect on angiogenesis in a Matrigel Plug Angiogenesis Assay. Further investigation revealed that
EAC's inhibition of cancer cell growth and invasion was also evident in a nude mice model. Our results
indicate that EAC inhibits the activation of NF-kappaB, and may provide a molecular basis for drug
development using EAC as an anti-invasive agent in the prevention and treatment of cancer.
PMID: 17316946 [PubMed - in process]
8: Food Chem Toxicol. 2006 Aug;44(8):1316-26. Epub 2006 Feb 28.
Apoptotic effects of Antrodia cinnamomea fruiting bodies extract are mediated through
calcium and calpain-dependent pathways in Hep 3B cells.
Kuo PL, Hsu YL, Cho CY, Ng LT, Kuo YH, Lin CC.
Department of Biotechnology, Chia-Nan University of Pharmacy and Science, Tainan, Taiwan.
Antrodia cinnamomea is well known in Taiwan as a traditional medicine for treating cancer and
inflammation. The purpose of this study was to evaluate the apoptotic effects of ethylacetate
extract from A. cinnamomea (EAC) fruiting bodies in Hep 3B, a liver cancer cell line. EAC decreased
cell proliferation of Hep 3B cells by inducing apoptotic cell death. EAC treatment increased the level
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of calcium (Ca2+) in the cytoplasm and triggered the subsequent activation of calpain and caspase-12.
EAC also initiated the mitochondrial apoptotic pathway through regulation of Bcl-2 family proteins
expression, release of cytochrome c, and activation of caspase-9 in Hep 3B cells. Furthermore, the
mitochondrial apoptotic pathway amplified the calpain pathway by Bid and Bax interaction and Ca2+
translocation. We have therefore concluded that the molecular mechanisms during EAC-mediated
proliferation inhibition in Hep 3B cells were due to: (1) apoptosis induction, (2) triggering of
Ca2+/calpain pathway, (3) disruption of mitochondrial function, and (4) apoptotic signaling being
amplified by cross-talk between the calpain/Bid/Bax and Ca2+/mitochondrial apoptotic pathways.( EAC() Hep 3B
EAC calpain caspase-12EAC
Bcl-2
cytochrome c caspase-9 Bid and
Bax calpain
EAC(1)(2) Ca2+/calpain pathway, (3)
(4) calpain/Bid/Bax Ca2+/
!)
Publication Types:
Research Support, Non-U.S. Gov'tPMID: 16600460 [PubMed - indexed for MEDLINE]
9: Chem Pharm Bull (Tokyo). 2006 Apr;54(4):496-500.
Protective effects of a neutral polysaccharide isolated from the mycelium of Antrodia
cinnamomea on Propionibacterium acnes and lipopolysaccharide induced hepatic injury in mice.
Han HF, Nakamura N, Zuo F, Hirakawa A, Yokozawa T, Hattori M.
Institute of Natural Medicine, University of Toyama, Japan.
Mycelia of Antrodia cinnamomea were extracted with chloroform and hot water. A neutralpolysaccharide named ACN2a separated from the water extract was purified using 10% CCl3COOH,
and repeated column chromatography on HW-65 and DE-52 cellulose. Its structure was determined by
chemical and spectroscopic analyses. ACN2a was composed of Gal, Glc, Fuc, Man and GalN (in the
ratio 1:0.24:0.07:0.026:faint), in which an alpha-D-(1-->6)-Gal linkage accounted for 73% of all
linkages. The ratio of branch points was about 16% of the total residual numbers, and branches were
attached to C-2 of galactosyl residues of the main chain. ACN2a had an average molecular weight of
12.9x10(5) Daltons, [alpha]D25=+115 degrees (c=0.44, H2O); [eta]=0.0417dl.g-1, Cp=0.2663 cal/(g.
degrees C). The hepatoprotective effect of ACN2a was evaluated using a mouse model of hepatic
injury that was induced by Propionibacterium acnes (P. acnes) and lipopolysaccharide (LPS). The
administration of ACN2a (0.4, 0.8 g/kg/d, p.o.), significantly prevented increases in serum aspartate
aminotransferase (AST) and alanine aminotransferase (ALT) enzyme activities in mice treated with P.
acnes-LPS, indicating hepatoprotective activity in vivo.
Publication Types:
Research Support, Non-U.S. Gov'tPMID: 16595952 [PubMed - indexed for MEDLINE]
J Ethnopharmacol. 2008 May 7. [Epub ahead of print]
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The adjuvant effects of Antrodia Camphorata extracts combined with anti-tumor agents on
multidrug resistant human hepatoma cells.
Chang CY, Huang ZN, Yu HH, Chang LH, Li SL, Chen YP, Lee KY, Chuu JJ.
Institute of Biotechnology, College of Engineering, Southern Taiwan University, Tainan, Taiwan;
Department of Medicine, Chi-Mei Medical Center, Yung-Kang City, Tainan Hsien, Taiwan.
AIM OF THE STUDY: The objectives of this study were to investigate the adjuvant anti-tumor effects
of Antrodia camphorate in human hepatoma cells (C3A and PLC/PRF/5) which are resistance to most
anti-tumor agents, elucidate the possible regulation pathways, and measure the tumor growth and
survival rate in xenograft-nude mice after combined with anti-tumor agents. MATERIALS AND
METHODS: The AC extracts were measured by using a phenol/sulfuric acid method as previously
described. The in vitro cell proliferation assay of ACs and anti-tumor agents was tested on C3A and
PLC/PRF/5 cell lines. The percentage of human hepatoma cells undergoing apoptosis and distributing
in different phases of cell cycle were determined by Flow cytometric analysis. Western blot analysis for
MDR-1 and apoptosis- related proteins. The measurements of tumor growth and survival analysis of
hepatoma implanted nude mice treated with Antrodia camphorata extracts and anti-tumor agents alone
or in combinations. RESULTS: We have found that Antrodia camphorata extracts, when combined
with anti-tumor agents, showed adjuvant antiproliferative effects on hepatoma cells (in vitro) and on
xenografted cells in tumor-implanted nude mice (in vivo), which then extended their median survival
days. Furthermore, solid-state extracts of Antrodia camphorata (AC-SS) showed its adjuvant effects
through the inhibition of MDR gene expressions and the pathway of COX-2- dependent inhibition of
p-AKT, which ultimately resulted in the induction of apoptosis in hepatoma cells. CONCLUSIONS: In
this study, we have found that Antrodia camphorata extract, when combined with anti-tumor agents,
showed adjuvant antiproliferative effects on hepatoma cells (in vitro) and on xenografted cells in
tumor-implanted nude mice (in vivo).
PMID: 18571350 [PubMed - as supplied by publisher]