JOBELYN

ANTI-OXIDANT, BLOOD-BOOSTER,

ANTI-INFLAMMATORY.

PRESENTER.

• Dr. David Abia-Okon MDCN (6500); AQ ( 2331)

Gen Surgeon .MD; SCS – Hungary.

• Medical Director. Lindabel Medical Centre Surulere Lagos.

• Medical Consultant. Health Forever Products Ltd. Ikeja Lagos.

BECAUSE.

•Worldwide, herbal medicines have been recognised to play vital roles in health care delivery systems.

BECAUSE.

The father of modern Medicine Hippocrates said 2500 yrs ago,

“Let food be thy medicine and Medicine from thy Food”

JOBELYN.• The HEMOGLOBIN / PCV booster.

• The powerful ANTIOXIDANT conglomerate.

• The selective anti-inflammatory agent.

Background• Jobelyn® is a herbal preparation from the leaves

of a tropical plant, Sorghum Bicolor; Guinea Corn;Dawa

• Originally marketed in Nigeria under the trade name Jubi Formula®,

• The component herb of Jobelyn® has been used by natives of S/West Nigeria, Sahel region,China, for many generations, to treat several diseases including pregnancy illness, sickle-cell anemia, leukemia, multiple myeloma, heart and other blood-related problems .

Jobelyn is 100% natural

• Five powerful antioxidant-compounds found in Jobelyn are used as markers for substance characterization.

• Their quantities in Jobelyn are substantial, explaining the outstanding antioxidant and anti-inflammatory potentials of Jobelyn.

• They are Luteolinidin, Apigeninidin, Luteolin. Apigenin and Naringenin.

POWERFUL

OBSERVATIONS.

Red Blood cell boosting effect

• Jobelyn increased HB

from 2.9g% to 5.3g%

(etiology….Post SS-crisis in 5yr-old)

In just 11days.Lindabel Medical Centre. Lawanson Surulere-Lagos.

SIMPLER ALTERNATIVES• Epogen, the popular synthetic erythropoeitin now has

some hitches according to FDA, USA.

• The FDA has focused its concern on study results showing an increased risk of death and tumor growth in chemo patients taking the anti-anaemia drugs.

• Erythropoietin is associated with an increased risk of adverse cardiovascular complications in patients with kidney disease if it is used to increase hemoglobin levels above 13.0 g/dl.[23]

JOBELYN contains 2 major types of

3-Deoxyanthocyanidins. Apigeninidin(47,380ug/g) and Luteolinidin (890ug/g)

3-DXAs are health promoting flavonoid-phytochemicals Are restricted to only a few plant species.

• Have antioxidant, anti-inflammatory and chemoprotective properties.

• Reduce the risks of CVDs and Cancer.The University of Hong Kong.

Prof.L.W.Rooney & Joseph Awika (Prof).

JOBELYNHas great potential for suppressing many cytokines,

IL-1;

IL-2;

IL-6..........known to trigger production of C-Reactive Protein.

IL-8;

PGE2;

TNF-alpha

Vivacell Biotechnology Gmbh, Germany ...2002.

JOBELYN•P8 an extract of Jobelyn, was found to

selectively inhibit the inflammation- enzyme COX-2, better than Ibuprofen.

• without the Vioxx and Celebex side effects.

Mdbiosciences USA.

Figure 2. Mean PGE2 production. PBMC’s were incubated with Test Articles for 1 hour prior to LPS stimulation (0.1 µg/ml). After 24 hours of LPS stimulation, supernatants were collected and analyzed for PGE2. Mean values are shown. Error bars represent standard deviations. PGE2 was analyzed by one-way ANOVA with Dunnett’s post test comparing sample values to the vehicle - LPS value or vehicle + LPS value. *P < 0.05.Above, before inflammation is induced, Jobelyn’s (P8), does not inhibit COX-1 which is responsible for the baseline levels of prostaglandin PGE2.

PGE2 Production (- LPS)

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JOBELYN• At the Military Hospital, Awolowo Rd. Ikoyi

Lagos, a limited clinical study of Jobelyn, showed that it increased the CD4 cells, either alone or used in collaboration with ARDs.

The general health condition of PLWHA was greatly improved when Jobelyn was introduced into their drug regimen.

(Publication of the XXXV International Congress on Military Medicine Infectious Diseases )

JOBELYN.• 3-amino acids Cystein, Glutamine,& Tryptophan

…….building blocks of

Selenoenzyme glutathione peroxidase,

the main Antioxidant, that boosts the immune potencial of the CD4-cells

……..also found in Jobelyn.

• (USDA National Nutrient Database for Standard Reference, Release 17(2004)

JOBELYN.• Powerful Antioxidants of the

……..Oligomeric Proanthocyanidin group, • ……..found in abundance in Jobelyn, • ……..Are known to block some entry

points of HIV into the CD4-cells.

(Chemokine Rec.Pts.2B,3,5)

Clinical trial at Lindabel Medical Centre Lagos , Nigeria ,

• 195 persons, chosen randomly, • 113 anemia cases (Hb below 12g%) discovered. • The levels of anemia varied from Hb 2.9g% to 8.4g%.

 

• On the average, a dose of one capsule of Jobelyn taken BD. yielded a minimum average increase of about 0.85g% over a period of 5 days in over 90% of the anemia patients treated.

Nutritional Facts per 250mg capsule.

• Calorific value 3 kJ (0.7 kcal)

• Dietary Fibre 34.9 % .....87.25mg

• Carbohydrate 23.8% 

• Protein 11.4% 

• Fat 0.1% 

DIETARY FIBRE.• FDA(USA) reommends atleast 20-35 mg daily.• Conditions linked to low fibre diets:• Constipation, Hemorrhoids, Diverticulitis, Irritable Bowel Syndrome, • Overweight and Obesity, • Coronary Heart Disease• Diabetes Mellitus,• Colon cancer.

British Nutrition Foundation. 2004..ROUPHAGE

• 250mg of Jobelyn contains 87.25 mg of Dietary Fibre

MethodsHaematological effect.

• In a controlled animal model (5 groups of 6 rabbits each; 0.94-1.46 kg body wt), anemia was induced in 3 groups of rabbits by mouse inoculation technique1 using a single dose of 0.25 ml of Trypanosoma b. brucei-infected blood of albino rat.

• As appropriate, some animals received 0.06 mg/kg of aqueous suspension of Jobelyn® daily.

• Uncoagulated blood samples of the rabbits were obtained weekly from the auricular vein for 7 weeks.

Methods

• Haematological effect (contd).• Packed cell volume (PCV) and haemoglobin

(Hb) concentrations were then determined within 24 h of collection using the microhaematocrit and cyanomethaemoglobin methods2.

• The differences in these hematological parameters were analysed statistically using ANOVA. (scientific analysis software)

Results• Haematological effect• Jobelyn® restored both PCV and Hb

levels to normal in the rabbits with T. b. brucei-induced anemia unlike anemic rabbits not treated with the herbal preparation which presented with a progressive decline in their PCV and Hb concentrations with majority (83.3%) dying before the end of the study.

• Healthy rabbits that received daily doses of the herbal preparation showed gradual elevation in PCV and Hb levels which were maintained within normal range (Figure 1).

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Group E

Normal Range

Figure 1: Changes in PCV levels of healthy and anemic rabbits. Group A, healthy controls; Group B, healthy rabbits treated with Jobelyn (day 1-49); Group C, T. b. brucei infected rabbits treated with Jobelyn (day 1-49); Group D, T. b. brucei infected rabbits treated with Jobelyn (day 10-49); Group E, T. b. brucei infected rabbits

Antioxidant effect• Phytochemical screening using TLC, HPLC and MS were carried out

for the isolation of relevant tannins and marker compounds present in the preparation.

• The oxygen radical absorbance capacity (ORAC) of Jobelyn® samples was determined using a multichannel liquid handling system coupled with a microplate fluorescence reader in 96-well format (Brunswick Lab Inc, USA).

• Trolox, a water-soluble vitamin E analog, was used as the calibration standard and the ORAC values were expressed as mmole Trolox equivalent (TE) per g.

• The ORAC values were compared with those of 13 fruits high in antioxidant properties.

RESULT on Antioxidant effect• The marker compounds, apigeninidin,

apigenin, luteolinidin, luteolin and naringenin, were isolated from the preparation in addition to proanthocyanidins ( bioflavonoid family) which are powerful antioxidants.

• The ORAC value of Jobelyn® was 3.123.0 µmole TE/g which was more than twice the total ORAC values for 13 antioxidant rich fruits (Table 1).

Table 1: ORAC values of 13 fruits

Fruit(ORAC) µmole

TE/g

Blueberries 24.00

Plums 9.49

Blackberries 20.36

Oranges 7.50

Frozen Tart Cherries 20.33

Red Grapes 7.39

Acai Berries 997.00

Strawberries 15

Dried Tart Cherries 68.00

Tart Cherry Juice Conc. 128.00

Canned Tart Cherries 17.00

Prunes 57.70

JOBELYN 3,123.00

Antioxidants in Jobelyn.

• SOD 31.8(kunitSODeq/g) • Phenolics  106.86 mg/g  • Apigenin 5,300 µg/g •  Luteolin  640 µg/g  • Apigeninidin 31,900 µg/g• Luteolinidin  331 µg/g• Naringenin 149 µg/g

Antiinflammatory effect• Human primary monocytes, isolated from

buffy coats of healthy human blood donors, were seeded in 24-well plates.

• Monocytes were incubated without or with lipopolysaccharide (LPS, 10 ng/ml),for 24 h in absence or presence of 6 doses of extract of Jobelyn® (1, 10, 50, 100, 250, and 500 µg/ml), which were added 30 min before LPS treatment.

Antiinflammatory effect (contd)

• After 24 h with the inflammatory stimulus, LPS (indirect assay for COX-2), or without LPS (indirect assay for COX-1), supernatants were removed, centrifuged and investigated for cytokines (IL-1beta, IL-6, IL-8 and TNFalpha) and prostaglandin E2 (PGE2) concentrations using ELISA with manufacturer’s protocol.

• COX selectivity was expressed as a ratio of the concentration of a test sample that inhibited 50% of the activity (IC50) of COX-2 to that of COX-1 and compared with selected medicines with antiinflammatory properties.

RESULTS on Antiinflammatory effects. • Jobelyn® significantly inhibited

lipopolysaccharide-induced release of cytokines (IL-1beta, TNFalpha, IL-6, IL-8) and PGE2.

• The product has a very high selective effect on Cox-2 than on Cox-1 activities with a selectivity index (IC50 Cox-2 : IC50 Cox-1) much lower than those of well known antiinflammatory agents (Table 2).

Table 2: Jobelyn selectivity for cyclooxygenases

Product Name

IC50 COX-1 µmole/litre

IC50 COX-2µmole/litre

Selectivity Index (COX-2:COX-1)

Tenidap 0.393 47.8 122

Diclofenac 0.000855 0.0019 2.2

Piroxicam 0.00527 0.175 33

Tenoxicam 0.201 0.322 15

Aspirin 1.7 270 167

Indomethacin 0.00021 0.00636 30

Jobelyn* 0.0238 0.0118 0.496

Ibuprofen* 11.267 209.0 18.91

*values of Cox-1 and Cox-2 were expressed as mg/ml

Conclusions • Jobelyn® is useful in the management of

anemia-related diseases.

• Its high Antioxidant values, makes Jobelyn, a promising medicine in the management of many diseases.

• The product is a likely suitable alternative to most currently used antiinflammatory medicines.

Some clinical trials.• reduce myocardial necrosis and reperfusion injury induced by free oxygen

radicals.

• relationship between antioxidant /dietary fibre intakes and coronary heart diseases (CHD)….

• Hemodialysis patients with prevalent CVD, supplementation with 800 IU/day vit. E. Antioxidant

• Inflammation and Atherothrombosis.• lower levels of CRP and IL-6 lead to retardation of coronary events. • chronic low-grade inflammation as cause of coronary heart disease,

Alzheimer's, and some cancers.• prevalence of coronary artery disease increased 1.5 fold for each doubling

of CRP level

3-DXA Nature’s Health Gift. From Sorghum Bicolor.

3-DXA are popular as health promoting phytochemicals. They are increasingly recognized for reducing the risks of Cardiovascular diseases and cancers due to their antioxidant, anti-inflammatory and chemoprotective properties.

• In the USA and other parts of the world scientists have been beaming their searchlights on Sorghum, which has been found to be the only dietary source of 3-DXA in large quantities.

Antioxidant rich-Diet.

• Such diets may reduce the plasma levels of lipid peroxide.

• Antioxidant rich foods may reduce myocardial necrosis and reperfusion injury induced by free oxygen radicals.

• Ref: J Am Diet Association 1995; 95:775-780. Singh et al.

Lipid Peroxidation

• Lipid peroxidation refers to the oxidative degradation of lipids.

• It is the process whereby free radicals "steal" electrons from the lipids in cell membranes, resulting in cell damage.

Reperfusion Injury• The damage of reperfusion injury is due in part to the inflammatory response

of damaged tissues.• White blood cells carried to the area by the newly returning blood release a

host of inflammatory factors such as interleukins as well as free radicals in response to tissue damage [1].

• The restored blood flow reintroduces oxygen within cells that damages cellular proteins, DNA & cell membrane. Damage to the cell's membrane may in turn cause the release of more free radicals.

• Reperfusion injury plays a part in the brain's ischemic cascade, which is

involved in stroke and brain trauma..This oxidation results in molecular oxygen being converted into lots of highly reactive superoxide and hydroxyl radicals.

Effect of consumption of foods rich in Antioxidants and dietary fibre on Coronary Heart Disease.

• Data obtained between 1984 and 1993 from 11629 men and women as part of the Scottish Heart Health Study (Scotland, UK) were used to investigate the relationship between antioxidant /dietary fibre intakes and coronary heart diseases (CHD)….

• The results suggest that the current public health drive to increase the consumption of fruits rich in antioxidants and dietary fibres will

impact positively on both CHD risk and the general health of the population.

• Ref: American Journal of Epidermiology.150(10):1073-1080, Nov.15 1999

Effect of Antioxidants in CVD and endstage Renal Disease.

• In Hemodialysis patients with prevalent CVD, supplementation with 800 IU/day vit. E reduces composite CVD endpoints and Myocardial infarction.

• Ref:The Lancet 2000; 356:1213-1218

Inflammation and Atherothrombosis.

• Inflammation is an important contributor to atherothrombosis.

• Elevated levels of one systemic marker of inflammation C-reactive protein (CRP), are associated with an increased risk of CVD events.

• Reduction of CRP levels, reduced vascular

inflammation as well as CVD events.

Antiinflammatories and ACS.

• When treated with Anti-inflammatory agents (rofecoxib plus aspirin),patients recovering from Acute Coronary Syndrome (ACS), had gradually reducing levels of CRP and IL-6.

• Suppression of inflammatory processes may lead to retardation of coronary atherosclerosis and coronary events.

Ref: New England Journal of Medicine, 2000;342:836-843.

INFLAMMATION causes all our problems.

• Almost overnight, medicine has been redefining inflammation as the root of most chronic diseases.

• Amazing as it may sound, the latest findings point to chronic low-grade inflammation as the underlying cause of coronary heart disease, Alzheimer's, and some types of cancer.

• Even obesity and diabetes have powerful undercurrents of inflammation.

Ref: New England Journal of Medicine, 2000;342:836-843.

WATCH THE INFLAMMATION MARKERS.

• That strong association makes C-RP a far more accurate predictor of heart-attack risk than either cholesterol or homocysteine.

• Vivacell Laboratories in Germany, demonstrated that Jobelyn suppressed the release of Interleukin-6, a cytokine that triggers the production of C-reactive protein.

Imagine, Jobelyn could improve the

function of your heart, increase your lifespan and ensure a healthy

life.

References 1. Erah PO et al. Afr J Biotech 2003; 2(9): 309-11. 2. Omotainse SO, Anosa VO. Rev. Elev Méd Vét. Pays Trop.

45:1992; 279-83. 3. Huang D et al. J Agric Food Chem. 2002; 50(16): 4437-44.

Jobelyn® is manufactured by Health Forever Product Limited, Lagos. Tel: +234-8033376135