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An Ideal IVF Lab – International / National Norms. Dr Aruna Saxena Lab Director, Lifecare IVF Pvt Ltd MD ( Obs & Gynae ) ARTCE ,National University of SINGAPORE Member ESHRE. An Ideal IVF Lab. http://icmr.nic.in/art/Chapter%20_2.pdf Primary Infertility care Unit / Level 1A & 1B - PowerPoint PPT Presentation
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An Ideal IVF Lab – International / National Norms
Dr Aruna SaxenaLab Director, Lifecare IVF
Pvt LtdMD (Obs & Gynae)ARTCE ,National
University of SINGAPOREMember ESHRE
An Ideal IVF Lab
NON STERILE AREA STERILE AREA
Waiting room Ovum pickup theatre
Consultation Room Embryo Transfer Room
Exam –Sonography Room Culture Laboratory
Semen collection room Cryopreservation room
IUI room
Sterilization room
Post operative ward
Library, Data record room, Store,AC
http://icmr.nic.in/art/Chapter%20_2.pdf Primary Infertility care Unit / Level 1A & 1B Secondary Infertility care Unit / Level 2 Tertiary Infertility care Unit / Level 3
Minimal Quality control parameters.PARAMETERS IN RELATION
TO/COMPARED TOACCEPTABLE RANGE
No of follicles No of eggs collected >90%
No of eggs Mature eggs Around 90%
Mature eggs (Inseminated/ Injected)
Normal fertilizationAbnormal fertilizationDegeneration
60-80%10-15%10-15%
Normal fertilization Cleavage rate 90%
Cleaved Usable 80%
No of embryos transferred
ET day ( 2-3) Max 3
Fresh ET cycle Cumulative pregnancy
30%
Frozen ET cycle Cumulative pregnancy
20%
ART cycles Freezable cycles 50%
Embryos thawed Survival rate 75%
Semen thawed Survival rate 50%
Minimal Quality control parametersParameters Vs Acceptable
range
Stimulation cycles
OHSS 0.2-8%
Total pregnancy
Multiple gestation
20-30%
Total pregnancy
Spontaneous Abortion
20-35 %
Total pregnancy
Ectopic pregnancy
Up to 8 %
Embryology for Clinicians
Immature eggMature egg with polar body
Embryology for CliniciansCleaved eggs, fragmentation
Hatching Blastocyst
THANK YOUTHANK YOU
Clinical embryologistMedical graduate or Post graduate in life
sciencesComplete supervision by a qualified and
experienced embryologist is mandatory for 6 months to 1 yr or first 100 cases
NO OF ART CYCLES NO OF EMRYOLOGISTS REQUIRED
1-150 2
151-300 3
301-600 4
>600 1 additional per 200 cycles
The Laboratory- minimum acceptable standardsMinimal personnel requirement :
ART CLINIC LABORATORY STAFF REQUIREMENT
Medical Director Laboratory Director
Clinician Supervisor
Sonologist Senior Embryologist
Anaesthetist Junior Embryologist
Counsellor Andrologist
Medical Secretory Lab technician
Nurses & cleaning staff
Lifecare IVF : Top of the line EquipmentsINSIDE OUTSIDE
Incubator Labotect C 200 & C16, Germany
Air filtration unit
Microscopes NIKON Phase contrast,SMZ 1000 & Inverted Microscope TiU, Japan
AC
Laminar airflow IVF tech Denmark Gas cylinders
Micromanipulator NarishiGe , Japan UPS
Cryo freezer , Cryo Bio systems , Germany
Generator
Our Datas
Application of Embryology to clinical practice
Immature eggs Hatching Blastocyst
