ambr® 250 modular High Performance Single-Use Benchtop Bioreactor System

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New Advanced Benchtop Bioreactor System for Parallel Fermentation or Cell Culture

ambr® 250 modular is an innovative new high performance benchtop bioreactor system for parallel fermentation or cell culture in 100 - 250 mL single-use bioreactors. The system utilizes the same advanced stirred tank bioreactor technology pioneered in the original ambr® 250 high throughput system. The system comprizes a series of elegant benchtop modules enabling 1 - 8 bioreactors to be operated in parallel and a control module with intuitive system software accessed via a user-interface screen.

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Productivity

Each single-use bioreactor vessel is fully integrated with sensors, feed reservoirs and syringe pumps which make it possible for experiments to be set up and turned around rapidly. One scientist can operate up to 8 bioreactors at a time. Both these factors allow lab productivity to increase significantly.

Scalability

Because the bioreactor vessels are geometrically similar to larger bioreactors, all processes on the system correlate empirically to those of large bioreactors making for optimum scalability.

Ease-of Use

The bioreactor vessels’ integral elements make it possible to focus on the process not the set-up. A user no longer needs to connect multiple tubes or filters or sterilize the vessels. The size and layout of the system also offer a uniquely ergonomic user experience.

Modular

The modularity of the system means that it can be extended to meet a growing company’s needs.

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New ambr® 250 modular

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ambr® 250 modular System Combines 1 - 8 Bioreactor Stations and a Control Module with System Software

Individial peristaltic pump Accessible for each bioraeactor, enabling substantial feeding and harvesting tasks. This is in addition to the 5 integrated syringe pumps in each bioreactor.

Bioreactor moduleHolds 2 bioreactor stations. Up to 4 modules can be connected to the controller allowing expansion of capacity from 2 bioreactor system to 8.

Bioreactor moduleUncluttered design- no bottles- no tubesPromoting reduction in errors.

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Touch screen user-interfaceEnabling easy control and supervision of multiple bioreactors.

Bioreactor controllerManages all processes including pH, temperature and DO.

Off-gas analyzer Monitors and controls processes using data from individual bioreactors’ exhaust gases.

Chilled feed reservoirChills media to temperatures between 6-8°C ensuring temperature sensitive media can be maintained.

3 step ‘Easy Connect’ installation for all gas, liquid and sensor connections - Step 1 - slot in - Step 2 - secure with clamp - Step 3 - secure pH connector Enabling quick set-up and rapid turnaround.

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ambr® 250 modular Bioreactor Vessels

Vessel scalabilityVessels are geometrically similar to standard bench and pilot scale bioreactors, enabling accurate scale up modelling.

Single-use syringe pumpsEach reservoir is integrated to its own high precision syringe pump allowing for highly consistent and accurate feed delivery.

Visible feed linesAllow view of liquid during auto-priming.

Single-use mammalian or microbial bioreactors- 100 - 250 mL working

volume.- Dual pitch blade or Rushton

impellers.- Spot based DO sensor.- Disposable pH electrode.- Integrated gas inlet filters.- Sparge and headspace

gassing options.- Integrated exhaust gas

condenser.

Integrated feed reservoirs- 2 Integrated 125 mL.

Reservoirs per bioreactor- 3 Integrated 50 mL.

Reservoirs per bioreactor

Each bioreactor is fully integrated with 5 liquid reservoirs and proprietary single-use syringe pumps. The integration simplifies experimental set-up, eliminates any need for vessel sterilization, and significantly reduces any error due to manual handling.

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Injectable septum capMakes rapid liquid additions with a syringe.

Gas tubeGases can either be delivered into the headspace or sparged into the media, these delivery systems are independent and can function in parallel.

Off-gas analyzer - microbial optionEach bioreactor has access to a dedicated analyser for CO2 and O2 analysis

Fully integrated feed lines and reservoirs Allowing for rapid experimental set-up and turnaround.

Single-use pH electrodePre-calibrated standard pH probe with a measurement range of 2 - 8.5.

Double impeller - Rushton or pitch-bladeFor microbial or mammalian vessels respectively.

Software

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ambr 250 modular is supplied with the same advanced software as the established ambr 250 high throughput system. Users can design new and complex experiments and analyze results easily and quickly. The software encompasses 4 distinct applications.

Process Definition To develop experimental protocols and algorithms from new or previously used templates. Simplifies management of all the reactor and reservoir controls including temperature, pH, DO and feed flow rates making it possible to design experiments of great complexity with minimal training.

Experiment ViewerThis historian application allows analysis of experiments completed on the ambr 250, presenting all the data generated including parameter profile, alarms and interventions. Displays experimental protocol and time in which steps were completed. This data rich platform provides all the information required for full understanding of experiments while enabling export and import of data to or from different sources.

RuntimeExecute and monitor up to 8 experiments simultaneously in real time. Add, delete or edit additional processes. The intuitive user-interface gives flexibility to correct or change experiments mid flow and with ease.

Results ViewerEnables user to analyze large data set generated from multiple completed experiments, including those from other bioreactor technologies and across the full suite of ambr products including ambr 15 and ambr 250. All data can be graphed on the same plot at the same time providing a comprehensive platform to view extensive data, allowing for fast and effective process analysis.

ambr® 250 modular automatically controls and feeds 1 to 8 bioreactor | fermenter vessels in parallel

Bioreactor controller- Three gasses per bioreactor

with mass flow sensor: - Mammalian - O2

- CO2

- N2 | air- Microbial - O2

- Air - N2

- Five positive displacement liquid pumps per bioreactor for high precision at low flow rates.

- Individual bioreactor temperature control with heating and cooling.

- Individual impeller speed control per bioreactor.

- Optional off-gas analysis by BioPAT ambr xgas for CO2 and O2.

Functions

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Applications

ambr® 250 modular is configurable for microbial fermentation or mammalian cell culture and able to model a wide range of requirements in a variety of applications across biopharma as well as industrial biotech.

- R&D and process development for biopharma

- R&D and process development for industrial biotech

- Process robustness experimentation in support of QbD studies

- Process development for vaccines

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Scalability

Single-Use from Cell Line and Process Development to Production Scale

- Geometrical similarity of vessel design

- Consistent mixing and gassing strategies

- Reliable single-use platform

ambr® 250 modular Univessel® SU 2L BIOSTAT® STR 50

Cells Mammalian, insect and stem cells | High cell density suspension cell cultures | Adherent cell cultures on microcarriers | Low to medium cell density microbial cultures

Process development

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BIOSTAT® STR 200 BIOSTAT® STR 500 BIOSTAT® STR 1000

Cells Mammalian, insect and stem cells | High cell density suspension cell cultures | Adherent cell cultures on microcarriers | Low to medium cell density microbial cultures

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