Agela

Agela Technologies — A Global Supplier for Chromatography Solutions

2008 Message From Agela Technologies

As Agela technologies is poised to enter its fifth year with confidence and pride in

its innovative separation, purification, and sample preparation products, we would

like to thank our many loyal customers for your continuous support and trust. With

your support and our effort in delivering the highest quality products to you, our

company has grown remarkably. This has allowed us to expand our research and

development effort, and thus introduce more innovative products to better service

your application needs.

In 2007, we had tremendous accomplishments: We cataloged over one thousand

different products. Our manufacturing and R&D operation was certified in compli-

ance with ISO 9001 and passed many quality audits by customers and distributors,

including VWR. As a global wide company, we not only have our own international

sales force but have also formed a marketing alliance with VWR international. This

will allow us to reach higher goals for 2008 and to provide our customers with even

better quality products and faster service.

Provide products with our INNOVATIVE technologies at the best PERFOR

MANCE to COST ratio.

Deliver products with guaranteed quality.

Provide global support with quick responses

Our 2008 mission statement and commitment:

How to Place Orders

Terms and ConditionsPLEASE READ THESE TERMS BEFORE ORDERING. IF YOU HAVE ANY QUESTIONS, PLEASE DO NOT HESITATE TO

CONTACT US AND OUR STAFF WILL BE GLAD TO ASSIST YOU.

Our office is open from 9:00 am to 6:00 pm Eastern

Standard Time, Monday through Friday.

To place an order or receive a quote, you may choose from

the following contacts:

Mail Address: Agela Technologies Inc.

625 DAWSON DRIVE, Newark, DE19713

Phone: (302) 438-8798

Fax: (302) 235-1833

Email: [email protected]

On-line: www.agela.com

Please include the following information with your order or

request: Account number (if you have one), purchase order

number, contact name, organization name, shipping and

billing address, telephone number, fax number or email

address, product number, brief description and quantity,

method of payment, preferred method of delivery. A written

confirmation will be sent to you by email or fax. We accept

business checks, wire transfers, and major credit cards as

methos of payment.

Checks:

Please make checks payable to:

Agela Technologies Inc. and send to:

Agela Technologies Inc.

625 DAWSON DRIVE, Newark, DE19713

Wire Transfer:

Please contact us by phone, fax or email for account

information.

Credit Cards:

Please include the card type and number, expiration date,

and card holder name. Due to security concerns, please

do not email the information. Please call or send a fax to

provide your credit card information.

Acceptance and Availability

All orders placed are subject to the agreement of Agela

Technologies Inc. The catalogue does not constitute an

engagement of the company to sell all listed products. You

are guaranteed to be notified at the time of ordering if the

ordered items are in back-order or discontinued.

Price and Payment

The prices are in effect at the time of printing. Agela

Technologies reserves the right to change the prices without

notice, though we do our best to provide our customers with

advance notice. The prices quoted at the time of ordering

will be guaranteed. The general payment term is net 30

days, F.O.B., Newark, Delaware,USA. However we reserve

the right to ask for prepayment if customers’ account

information is not satisfactory. A 1.5% per month service

charge will be added to delinquent accounts. If a purchase

order is less than $1000.00, a $50.00 extra charge will be

added to the invoice.

Changes

Agela Technologies reserves the right to change product

specifications, quantities, designs or prices without prior

notice and without liability for such changes.

Shipping Policy

The standard shipping method is 2 day FedEx within the

United States and Canada. We will try to accommodate

requests for other shipping methods if they are available. All

shipping and handling charges will be billed separately.

Should you receive damaged goods, it is imperative that

you notify us immediately and save all packing materials for

inspection by the carrier.

Application

All products in this catalog should be used for laboratory or

manufacturing use only. They are not intended for direct

medicinal or food use. Agela Technologies assumes no

liability for any misuse of the products.

Returns

Agela Technologies tries to accommodate all requests for

returns of unused goods. However,return of some items

may be restricted by the original manufacturers. Please

contact us for return authorization before returning any

items. A restocking charge may be applied to certain

products.

Warranty

All Agela Technologies products are warranted to be free of

defects in materials and workmanship. They are not

warranted for any other particular purpose. Agela Technolo-

gies shall not under any circumstance be liable for any

incidental, consequential or compensatory damage in

conjunction with its products. The maximum liability shall

not exceed the invoice price of the product.

HPLC Accessories and Supplies

SPE Accessories and Supplies

GC Accessories and Supplies

Filtration Products

Vials and Caps

Cleanert SPE Columns,Wellplates and Media

The Development of SPE Method

Applications

Cross-reference Table

Introduction

HPLC Columns by Family

Selected Solutions for Popular Applications

Ordering Information by the Type of Stationary Phases

HPLC Column Selection Guide

Applications

Appendix

002

002

033

044

054

058

075

HPLC Columns and Media

SPE Columns and Wellplates

Purification Products

GC Columns

Accessories and Supplies146

153

157

162

166

127

078

103

106

114

Preparative HPLC Columns

Flash Chromatography Media and Columns

Flash Chromatography System

116

120

125

HPLC Columnsand Media

Introduction


Unisol Technology and Column Products–NEW

Optimix Technology Column Products–NEW

Durashell Technology and Column Products –NEW

Venusil Family of HPLC Columns

Promosil HPLC Columns

Other Manufacturers’ Brands


Solutions for Highly Water Soluble Compounds

Solutions for Low pH and High pH Applications

Solutions for LC-MS

Solutions for Fast Analysis

Solutions for Bio-molecules

Solutions for Preparative HPLC

Solutions for SFC


Reverse Phase Columns

Normal Phase Columns

Ion-exchange Columns

Mixed Phase Columns

Size Exclusion Columns (Silica Based)

Polymer Phase Columns


Applications

Appendix

002

002

002

011

015

017

031

032

033

033

039

040

042

043

043

043

044

044

047

049

050

051

051

054

058

075

HP

LC

Colu

mns

002

AgelaTechnologiesBETTER SOLUTION FOR CHROMATOGRAPHY

As one of the emerging leaders in chromatography technology, Agela Technologies proudly presents five new HPLC columns developed

using our latest cutting-edge technologies. These products exhibit superior performance and are designed to meet the ever-increasing

demands of our customers.

Agela’ s unique sol-gel process technology generates a uniform surface that produces a universal column applicable to many HPLC applications.

Peak tailing adversely affects chromatographic efficiency and reduces the resulting accuracy and precision of the data. Peak tailing and

insufficient retention of polar compounds are among the toughest problems for silica based reverse phase columns. High acidity of the

surface silanols, non-uniformity of the surface, and non-uniformity of the bonding process are the three major causes of peak tailing.

High purity synthetic silica is typically obtained by hydrolyzing silicic acid esters followed by high temperature sinteration. The sintered silica

is further leached with dilute acid to enrich silanol on the surface. The process does not generate a uniform, silanol covered surface. It will

create some isolated silanol groups with very strong acidity or some “un-leached islands” during the leaching process. Furthermore, the high

temperature procedure may cause formation of micro-crystallization domains on the amorphous silica, further contributing to the non-

uniformity of the surface.

Unisol process technology is specifically designed to solve these problems. In our patented Unisol process, gas phase tetramethoxy silane is

adsorbed evenly on the silica surface. It is subsequently hydrolyzed by a controlled amount of moisture. A fresh layer of silanols is then

formed by a mild heating process. This process produces a surface with lower acidity and high uniformity, which is further bonded with a high

purity C18 silane, and then di-functional end-capped. The overall process yields an excellent HPLC stationary phase with a very smooth

surface that is friendly to basic compounds (minimal ionic interactions) and polar compounds (provides significantly increased retention).

Major benefits of the Unisol Technology for reverse phase and normal phase chromatography:

1.Unisol C18 - A type of column that is a true universal reverse phase column for the separation of acidic, basic, and neutral molecules. It

has superior performance to any conventional C18 phases in almost every aspect.

2.Unisol Amide - A unique phase that is very different from other stationary phases in its separation mechanism. It can be used as a RP or

a NP column. It has superior performance than any of the other HILLIC columns on the market. It has superior retention for polar com-

pounds when operated as a HILIC column.

3.Optimix columns – A class of columns that provide optimized selectivity and performance for HPLC separations. It has balanced hydro-

philicity and hydrophobicity: extended retention for polar compounds; non-excessive retention for strong hydrophobic compounds.

4.Durashell columns - A class of column that can be used in a wide pH range of 1-12.

5.Venusil ASB columns – A class of columns that are excellent for low pH and LC-MS applications.

●Greatly improved peak shape for basic compounds

●100% water compatibility

●Significantly increased retention for highly water soluble compounds and balanced hydrophobicity in the reverse phase mode

●Elimination of excessive retention for polar compounds in the normal phase mode ( “hot spots” )

Unisol Technology and Column Products NEW in HPLC !


Introduction

HP

LC

Colu

mns

004


Balanced Retention for Hydrophilic and Hydrophobic Compounds

Mobile Phase:35% 20 mM KH2PO

4 pH=7.0, 65% Methanol

Temperature: 23

Flow: 1mL/min

Detection: UV 254 nm

Sample: Uracil

Propranolol hydrochloride

Butyl Phthalate

Dimethyl Phthalate,

Naphthalene

Acenaphthene

Amitriptyline

Extremely Low Metal Effects

Mobile Phase: A: Water, B: Methanol; A:B = 65:35

Flow Rate: 1 mL/min

Temperature: 25


Sample: 2,3-Dihydroxynaphthalene 2,7-Dihydroxynaphthalene

Unisol C18 5μm, 4.6mm×150mm Brand W, AQ Column 5μm, 4.6mm×150mm

Brand H, C18 Column 5μm, 4.6mm×150mm Brand Z,C18 Column 5μm, 4.6mm×150mm

min0 1 2 3 4 5 6 7

mAU

0

10

20

30

40

VWD1 A, Waveleng th=230 nm (LPF\07091702.D)

3.0

08

4.0

84

TF=1.31

min0 1 2 3 4 5 6

mAU

0

10

20

30

40

50

VWD1 A, Wavelength=230 nm (LPF\07090605.D)

Are

a: 2

53.2

88

2.2

75

Are

a: 4

00.3

99

2.8

89

TF=1.40

min0 10 20 30 40 50 60

mAU

0

100

200

300

400

500 VWD1 A, Wavelength=254 nm (HLX\08021110.D)

1.6

41

3.2

17

6.4

97

8.2

33

14

.13

7

31

.06

1

48

.84

2Unisol C18 ,5μm, 4.6*150mm

Part No.: UO951505-0

min0 10 20 30 40 50 60

mAU

0

100

200

300

400

VWD1 A, Wavelength=254 nm (MSW\08013140.D) 1.6

50

3.5

83

7.5

30

9.1

30

16

.62

1

37

.59

9

58

.32

2

Brand W C18, 5μm, 4.6*150mm

min0 10 20 30 40 50 60

mAU

0

100

200

300

400

VWD1 A, Wavelength=254 nm (MSW\08020100.D) 1.9

09

3.0

06

5.6

16

6.7

32 7

.42

1

14

.01

2

60

.33

9

Brand Z C18, 5μm, 4.6*150mm

min0 1 2 3 4 5 6 7

mAU

0

5

10

15

20

25

30

35

40

VWD1 A, Waveleng th=230 nm (LPF\07091208.D)

2.4

50

3.4

71

min0 2 4 6 8 10

mAU

5

10

15

20

25

VWD1 A, Wavelength=230 nm (LPF\07091202.D)

3.9

44

Are

a: 1

76.6

86

5.9

34

TF=1.69 TF=2.88

HP

LC C

olum

ns

003

Unisol C18

A Slightly Polar, 100% Water Compatible and Universal Reverse Phase

A unique and universal C18 HPLC phase made by our patented Unisol Technology. Unprecedented separation performance for compounds

with a wide range of properties from hydrophilic to hydrophobic: polar, semi-polar and non-polar compounds.

Characteristics: Metal Impurity < 10 ppm; Pore Size 100 Å; Specific Surface Area 410 m2/g; Carbon Loading 17%; Single end-cap; Avail-

able Particle Size: 2.5 μm, 3μm, 5μm, 10μm

Consider this column as the first option for your HPLC method development

●One of the most universal reverse phase columns

●Great inertness and efficiency for basic compounds

●Enhanced retention of hydrophilic compounds

●100% aqueous compatibility

●Robust and reproducible performance

●Wide pH range (1.5-9.0)

●Low bleed and high sensitivity for LC-MS

Sample: uracil, toluene, doxepin, amitriptyline

Column: 4.6 x 150mm, 5 μm

Mobile phase:10 mM sodium phosphate ( pH =7 ) in 60% ACN.

Temperature: 30 ; Flow rate: 1 mL /min.

The plate numbers in regular font are for toluene, the plate

numbers in Italic are for amitriptyline

Great Inertness and Efficiency for Basic Compounds

Best ValueGuaranteed Product QualityInnovation to Benefit Customers

YMC AQ Column4.6×250mm,5μm

Mobile Phase A: Water (Contain 0.01mM Na

2PO

4 and 0.01mM Citric Acid, pH=4.5 )

B: ACN , A:B =95:5Sample: Cytosine, Uracil, Guanine

Temperature 35Detection 254 nm

Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556

Waters-Atlantis

12071

14695

4010 12083

Unisol C18

Brand K C18

Inertsil C18

Brand A C18

Waters-Xterra C18

1638

14316

13792

9518

15307

4533

12936

6772

Good Retention for Polar Compounds

Unisol C184.6×250mm,5μm


HP

LC

Colu

mns

005

Sample: uridine in calf blood plasma (diluted 20 times with 0.1%

formic acid)

Injection: 1μl (not be cleaned up with SPE)

Column: Unisol C18 2.1×50mm,5μm

Temperature: 30

Flow Rate: 0.3mL/min

Mobile phase:0.1% formic acid solution (100% aqueous phase)

Run time: 8 min

After 500 injection, wash with methanol /0.1% formic acid solution =

50/50 for 30mins column temperature 40 ; rinse with 95%MeOH for

30mins, column temperature 45

Good Tolerance of Directly Injected Biological Matrix

Best Value

Guaranteed Product Quality

Innovation to Benefit Customers

0 1 2 3 4 5 6 7 min

0 1 2 3 4 5 6 7 min

0 1 2 3 4 5 6 7 min

mAU60

50

40

30

20

10

0

-10

Fresh Colunm

Plates = 3224

mAU60

50

40

30

20

10

0

-10

After column washing and re-equlibrium in

100% water (0.1% formic acid)

Plates = 3045

mAU60

50

40

30

20

10

0

-10

After 500 sample injections

Plates = 2810

Analysis of Quaternary Ammonium Alkaloids from Copitidis with a

Non-Volatile Ion Pair Reagent using Brand Z C18 Column.

Mobile Phase: ACN:0.05 mol/L KH2PO

4 Aq.

(40 60, Contain 0.015 mol/L SDS)

Analysis of Quaternary Ammonium Alkaloids from Copitidis without a

Non-Volatile Ion Pair ReagentUsing Unisol C18 Column .

Mobile Phase : Water + 0.2 TFA :acetonitrile= 75:25

Enable Methods to be Compatible with LC-MS

Ordering Phone No.: (302) 438 8798

Support Phone No.: (302) 588 4556

0

0

50

100

150

200

250

5 10 15 20 25 30 35 40

mAU

min

0.00

0.00

0.05

0.10

0.15

0.20

0.25

5.00 10.00 15.00 20.00 25.00 30.00 35.00 40.00 45.00 50.00

HP

LC

Colu

mns

006


Compatibility with 100% Aqueous Mobile Phase

mAU

20

10

0

0 2 4 6 8 10 12 14 16 18 min

0 2 4 6 8 10 12 14 16 18 min

0 2 4 6 8 10 12 14 16 18 min

0 2 4 6 8 10 12 14 16 18 min

0 2 4 6 8 10 12 14 16 18 min

0 2 4 6 8 10 12 14 16 18 min

Unisol C18 , initial

mAU

20

10

0

Unisol C18

after 4 hours

mAU

20

10

0

Luna C18 initial

mAU

200

100

0

Brand K C8 initial

mAU

200

100

0

Luna C18 after 4 hours

mAU

400

200

0

Brand K after 4 hours

Sample: Uridine

Column: 4.6 x 150 mm,5 μm

Mobile phase:100% water

Flow rate: 1mL/min; the flow was stopped for 5 minutes

during the testing period for each column.

Temperature: 30oC

Greater Volume Loading Capacity Than Other Brands

Large Volume Injection (Injection volume =100 μL)

Sample: 4-methoxybenzenesulfon amide,

10 μg/mL in MeOH/Water (1:1)

Column: 4.6 x 150 mm, 5μm

Mobile phase: MeOH/Water

Flow rate: 1mL/min

Temperature: 30 oC

min0 1 2 3 4 5

mAU

0

50

100

150

200

min0 1 2 3 4 5

mAU

0

50

100

150

200

0

50

100

150

200

min0 1 2 3 4 5

mAU

Unisol C18, Plates=9137

Brand A-C18, Plates=2029

Brand Z-C18, Plates=2118

HP

LC

Colu

mns

007

Best Value



Stability of Unisol C18 at pH=1.5

Sample: Uracil, Doxepin, Amitriptyline,Toluene.

Column: Unisol C18, 4.6 x 150 mm, 5 μm

Mobile phase: TFA (0.25%, pH 1.5) in 60% acetonitrile

Flow rate: 1 mL/ min.;

Temperature: 40 oC

mAU

40

30

20

10

0

0 1 2 3 4 5 6 7 8 9 min

After 4 hours

0 1 2 3 4 5 6 7 8 9 min

mAU

40

30

20

10

0

After 500 hours

Sample: Uracil, Doxepin, Nortriptyline,Amitriptyline, Trimipramine

Column: Unisol C18, 4.6 x 250 mm,5 μm

Mobile phase: 0.01 M sodium phosphate : ACN = 25 : 75, pH=7.0

Flow rate: 1.3 mL/min.

Temperature: 30oC

mAU

40

35

30

25

20

15

10

5

00 5 10 15 20 25 30 min

2.08

0 2.32

0

4.06

8

6.96

1

9.77

5 14.2

76

Initial

mAU

40

35

30

25

20

15

10

5

00 5 10 15 20 25 30 min

2.04

0 2.31

1

4.33

7

6.09

0

9.96

5 14.2

71

After 150 hours

Perfect Peak Symmetry and Good Stability at Mid-pH for Basic Compounds



HP

LC C

olum

ns

008


Unisol Amide (HILIC)

Unisol Amide stationary phase is made from high purity spherical silica particles bonded with a neutral hydrophilic amide group. It can be

used for normal phase reverse phase, or hydrophilic interaction HPLC. The Unisol Amide (NP) column is a good replacement for NH2 and

Silica columns that are currently marketed as HILIC. Compared with traditional silica and NH2 columns, the Unisol Amide columns have

better reproducibility and column lifetime. It is especially useful for the separation of strong hydrophilic compounds, whether they are acidic,

basic or neutral. It is compatible with aqueous mobile phases in the pH range of 2-8. It has selectivity very different from most of conven-

tional stationary phases

Characteristics: Metal Impurity < 10 ppm; Pore Size 100 Å; Specific Surface Area 410 m2/g; Carbon Loading 6%; Single End-cap; Available

Particle Size: 3μm, 5μm, 10μm

Main features:

●Strong retention of polar compounds in HILIC mode

●Very unique selectivity and good complementary to the conventional reverse phase

●Can be used as reverse phase, normal phase and HILIC phase

●More robust and reproducible performance than silica and amino stationary phase

●100% aqueous and 100% organic solvents compatible

●pH range (2-8)

Separation of Validamycin

Mobile Phase: A: 0.1% TFA in WaterB: Acetonitrile

Gradient: 40% A to 85% A in 30 min

Flow Rate: 1 mL/min

Temperature: 25


Sample: Validamycin

Mobile Phase: A: 0.005M NaH2PO

4pH=7) ; B:MeOHA:B=98:2

Flow Rate: 1 mL/min

Temperature: 25


Sample: Validamycin

OONH

CH2OH

HOH2COH

OH

CH2OH

OHHO

OH

OH

HO OH

Column: Unisol Amide,5μm, 4.6mm×250mm

Part No.: UA952505-0

Column:Brand W ,

AQ Column,5μm, 4.6mm×250mm

Au0.12

Au0.20

0.18

0.16

0.14

0.12

0.10

0.08

0.06

0.04

0.02

0.00

0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 min

-0.02

0.10

0.08

0.06

0.04

0.02

0.00

-0.02

2.00 4.00 6.00 8.00 10.00 12.00 14.00 16.00 18.00 min

HP

LC

Colu

mns

009



Best Value



m in0 1 2 3 4 5 6

m AU

0

1 0 0

2 0 0

3 0 0

4 0 0

5 0 0

D AD 1 A , S ig = 28 0 ,4 R e f= 3 6 0 ,1 0 0 (AG E LA\0 7 01 3 0 0 0 .D )

1.9

57

2.3

00

3.3

92

5.5

73

m in0 1 2 3 4 5 6

m A U

0

5 0

1 0 0

1 5 0

2 0 0

2 5 0

D A D 1 A , S ig = 2 8 0 ,4 R e f = 3 6 0 ,1 0 0 ( A G E L A \0 7 0 2 1 1 0 9 .D )

1.6

58

1.7

68

3.5

73

4.6

79

m in0 1 2 3 4 5 6

m A U

0

5 0

1 0 0

1 5 0

2 0 0

D A D 1 A , S ig = 2 8 0 ,4 R e f= 3 6 0 ,1 0 0 (A G E L A \0 7 0 2 1 1 1 4 .D )

3.2

08

3.4

87

3.8

35

4.4

04

Separation of Shikimic Acid

Column: Unisol Amide, 5 μm, 4.6mm×150mm


Mobile Phase:A: 1% Fomic Acid in Water B: Acetonitrile


Flow Rate: 1 mL/min

Temperature: 25


Sample: Shikimic Acid

Mobile Phase:A: 0.1% TFA in Water B: 0.1% TFA in Acetonitrile

A: B = 90:10

Flow Rate: 1 mL/min

Temperature: 30


Sample: VB1+VB6+VC+VB2

Water-soluble Vitamins

Unisol Amide 5 μm, 4.6mm×150mm


Brand W Silica Column, 5μm, 4.6×150mm

Brand W, NH2 Column, 5 μm, 4.6×150 mm

HP

LC C

olum

ns

010


Ordering Information

Surface Area: 410 m2/g, Pore Size: 100Å

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Gaurd cartridge

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Gaurd cartridge

Semi-preparative

Semi-preparative

Preparative

Preparative

Preparative

Preparative

Preparative

Preparative

Guard cartridge

2.1×30

2.1×50

2.1×100

2.1×150

4.6×50

4.6×100

4.6×150

4.6×250

4.6×10

2.1×30

2.1×50

2.1×100

2.1×150

4.6×50

4.6×100

4.6×150

4.6×10

10×150

10×250

20×50

20×150

20×250

30×100

30×150

30×250

20×20

UO950302-0

UO950502-0

UO951002-0

UO951502-0

UO950505-0

UO951005-0

UO951505-0

UO952505-0

UO950105-0

UO930302-0

UO930502-0

UO931002-0

UO931502-0

UO930505-0

UO931005-0

UO931505-0

UO930105-0

UO951510-0

UO952510-0

UO950520-0

UO951520-0

UO952520-0

UO951030-0

UO951530-0

UO952530-0

UO950220-0

VH950302-0

VH950502-0

VH951002-0

VH951502-0

VH950505-0

VH951005-0

VH951505-0

VH952505-0

VH950105-0

VH930302-0

VH930502-0

VH931002-0

VH931502-0

VH930505-0

VH931005-0

VH931505-0

VH930105-0

VH951510-0

VH952510-0

VH950520-0

VH951520-0

VH952520-0

VH951030-0

VH951530-0

VH952530-0

VH950220-0

5

5

5

5

5

5

5

5

5

3

3

3

3

3

3

3

3

5

5

5

5

5

5

5

5

5

2.1×30

2.1×50

2.1×100

3.0×30

2.5

2.5

2.5

2.5

UO920302-0

UO920502-0

UO921002-0

UO920303-0

3.0×50

3.0×100

4.6×50

4.6×100

2.5

2.5

2.5

2.5

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Fast analysis

3

3

3

3.0×50

3.0×100

3.0×30

3.0×50

3.0×100

3.0×30 5

5

5

Analytical

Analytical

Analytical

UO930303-0

UO930503-0

UO931003-0

UO950303-0

UO950503-0

UO951003-0

UO920503-0

UO921003-0UO920505-0

UO921005-0

VH950303-0

VH950503-0

VH951003-0

VH930303-0

VH930503-0

VH931003-0

Type Dimension (mm) Particle Size(µm) Unisol C18 Unisol Amide(HILIC)

HP

LC C

olum

ns

011

Conventional silica based HPLC stationary phases are usually bonded with mono-type alkyl chain molecules, e.g., C18, C8, CN, etc. Recent

studies revealed that synergistic effects can be obtained when the bonded surface consists of mixed phases of C18 and a shorter chain (C8,

amide, CN, nitro-phenyl, etc). These new products offer different selectivity, coordinated multi-functionality, as well as a spacer effect

(C18/C8).

It is difficult to produce a uniformly bonded C18 surface on silica due to steric hindrance of the bulky C18 chain. This problem can be easily

overcome when the surface is bonded with mixed chain lengths, C18 and a shorter silane. As a result, there are many advantages:

improved surface uniformity, better penetration of molecules during partitioning due to less steric hindrance, and different selectivity com-

pared to a mono-chain type stationary phase.

As one of the leaders in separation technologies, Agela Technologies has innovatively developed a family of non-ionic, mixed-phase

HPLC/SFC columns.

Consider these columns as options for your HPLC method development

Improved performance from a mixed-phase C18/C8 column compared to mono-chain C18 or C8 columns

●Alternative selectivity

●Improved selectivity for stereo isomers, better differentiation of shapes

●Better peak shape due to uniformity of the bonding.

●Balanced hydrophilicity and hydrophobicity: extended retention for polar compounds; non-excessive retention for strong hydrophobic compounds

Characteristics: Metal Impurity < 10 ppm; Pore Size: 100 Å; Specific Surface Area: 410 m2/g; Carbon Loading: 16%; Double End-cap; Avail-

able Particle Size: 3µm, 5µm, 10µm.

NEW in HPLC !Optimix Technology Column Products



Optimix C18/C8:

Analysis of Vancomycin and its impurity

Vancomycin ImpurityNH

2

H3C

HO

CH3

OHHO

OH

OH

CH3

CH3

CI

CI

OO

OO

OO

O

O

O O

O

O

O

HO

HO

HOOC

H

H H

H HH

3C

NH2

HHHHN

NH

HN

NH

NH

HN

HN

H

OHOH

NH2

H3C

HO

CH3

OHHO

OH

OH

CH3

CH3

CI

OO

OO

OO

O

O

O O

O

O

O

HO

HO

HOOC

H

H H

H HH

3C

NH2

HHHHN

NH

HN

NH

NH

HN

HN

H

OHOH

HP

LC

Colu

mns

012


Column: Optimix C18 /C8,5μm 4.6×250mm

Part No.: OP952505-OC

Mobile Phase:A: 0.7% TEA and 3% Acetic Acid in Water

B: Methanol ;C:THF ;A:B:C(88:10:2)

Flow Rate: 1 mL/min

Temperature: 25


Sample: Vancomycin Impurity

Vancomycin

Impurity

Column: Brand W C18 Column,5μm , 4.6×250mm

Mobile Phase:USP 24

A: 0.2% TEA (pH3.2)-Acetonitrile-THF(92:7:1)

B: 0.2% TEA (pH3.2)-Acetonitrile-THF(70:29:1)

Gradient USP 24

Time(min) 0 12 20 22 23 30

B% 0 0 100 100 0 0

1.0e+2

9.5e+1

9.0e+1

8.5e+1

8.0e+1

7.5e+1

7.0e+1

6.5e+1

6.0e+1

5.5e+1

5.0e+1

4.5e+1

4.0e+1

3.5e+1

3.0e+1

2.5e+1

2.0e+1

1.5e+1

1.0e+1

5.0

5.00 10.00 15.00 20.00 25.00Time

12.52

11.52

Impurity

Vancomycin

1.0e+2

9.5e+1

9.0e+1

8.5e+1

8.0e+1

7.5e+1

7.0e+1

6.5e+1

6.0e+1

5.5e+1

5.0e+1

4.5e+1

4.0e+1

3.5e+1

3.0e+1

2.5e+1

2.0e+1

1.5e+1

1.0e+1

5.0

20.0017.5015.0012.5010.007.50Time

HP

LC C

olum

ns

013


Optimix C18/Amide

A mixed-phase of C18 and propionic amide provides unique selectivity which allows extremely polar and non-polar compounds to be ana-

lyzed in a single run under isocratic conditions.

Characteristics: Metal Impurity < 10 ppm; Pore Size: 100 Å; Specific Surface Area : 410 m2/g; Carbon Loading : 11%; Single End-cap;

Available Particle Size: 3 µm, 5 µm

Performance Features:

●Unique selectivity

●Balanced retention for extremely polar and non-polar compounds

●Greater retention for polar compounds due to coordinated effects of C18 and amide

●Minimal tailing for basic compounds compared to any other type of columns, due to suppression of silanol effects by the amide group.

Bring extremely polar and non-polar analytes closer to shorten the analysis time under isocratic conditions

Mobile Phase A: Water, B: Methanol

Sample: Uracil, Toluene

Flow rate: 1 mL/min

Detection: UV = 254 nm

t0= 1.52 min

Column: Optimix C18/amide,5μm, 4.6×150mm

Mobile Phase: A : B = 50 : 5

Column: C18, 5μm,4.6×150mm

Mobile Phase: A : B = 60 : 40


t=2.023 min

t= 15.278 min

t=1.658 min

t=57.216 min

HP

LC C

olum

ns

014



Surface Area: 410 m2/g, Pore Size: 100Å

Type Dimension (mm) Particle size(µm) Optimix C18/SCX Optimix C18/SAX Optimix C18/Nitrophenyl

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Guard cartridge

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Guard cartridge

2.1×30

2.1×50

2.1×100

2.1×150

4.6×50

4.6×100

4.6×150

4.6×200

4.6×250

4.6×10

2.1×30

2.1×50

2.1×100

2.1×150

4.6×50

4.6×100

4.6×150

4.6×10

OP950302-SC

OP950502-SC

OP951002-SC

OP951502-SC

OP950505-SC

OP951005-SC

OP951505-SC

OP952005-SC

OP952505-SC

OP950105-SC

OP930302-SC

OP930502-SC

OP931002-SC

OP931502-SC

OP930505-SC

OP931005-SC

OP931505-SC

OP930105-SC

OP950302-SA

OP950502-SA

OP951002-SA

OP951502-SA

OP950505-SA

OP951005-SA

OP951505-SA

OP952005-SA

OP952505-SA

OP950105-SA

OP930302-SA

OP930502-SA

OP931002-SA

OP931502-SA

OP930505-SA

OP931005-SA

OP931505-SA

OP930105-SA

OP950302-NP

OP950502-NP

OP951002-NP

OP951502-NP

OP950505-NP

OP951005-NP

OP951505-NP

OP952005-NP

OP952505-NP

OP950105-NP

OP930302-NP

OP930502-NP

OP931002-NP

OP931502-NP

OP930505-NP

OP931005-NP

OP931505-NP

OP930105-NP

5

5

5

5

5

5

5

5

5

5

3

3

3

3

3

3

3

3

Type Dimension (mm) Particle size(µm) Optimix C18/C8 Optimix C18/amide Optimix C18/phenyl Optimix C18/CN

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Guard cartridge

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Guard cartridge

2.1×30

2.1×50

2.1×100

2.1×150

4.6×50

4.6×100

4.6×150

4.6×200

4.6×250

4.6×10

2.1×30

2.1×50

2.1×100

2.1×150

4.6×50

4.6×100

4.6×150

4.6×10

OP950302-OC

OP950502-OC

OP951002-OC

OP951502-OC

OP950505-OC

OP951005-OC

OP951505-OC

OP952005-OC

OP952505-OC

OP950105-OC

OP930302-OC

OP930502-OC

OP931002-OC

OP931502-OC

OP930505-OC

OP931005-OC

OP931505-OC

OP930105-OC

OP950302-AM

OP950502-AM

OP951002-AM

OP951502-AM

OP950505-AM

OP951005-AM

OP951505-AM

OP952005-AM

OP952505-AM

OP950105-AM

OP930302-AM

OP930502-AM

OP931002-AM

OP931502-AM

OP930505-AM

OP931005-AM

OP931505-AM

OP930105-AM

OP950302-PH

OP950502-PH

OP951002-PH

OP951502-PH

OP950505-PH

OP951005-PH

OP951505-PH

OP952005-PH

OP952505-PH

OP950105-PH

OP930302-PH

OP930502-PH

OP931002-PH

OP931502-PH

OP930505-PH

OP931005-PH

OP931505-PH

OP930105-PH

OP950302-CN

OP950502-CN

OP951002-CN

OP951502-CN

OP950505-CN

OP951005-CN

OP951505-CN

OP952005-CN

OP952505-CN

OP950105-CN

OP930302-CN

OP930502-CN

OP931002-CN

OP931502-CN

OP930505-CN

OP931005-CN

OP931505-CN

OP930105-CN

5

5

5

5

5

5

5

5

5

5

3

3

3

3

3

3

3

3

HP

LC C

olum

ns

015


Durashell Technology and Column Products

Agela’ s Durashell column has a pH range of 1-12. Different from the current marketed products that also can be used at high pH,

Durashell’ s stationary phase is made with a dentrimer technology followed by molecular modifications. Agela’ s technology produces a

strong hydrophobic protection layer over the silica surface allowing the columns to be used at extremely high and low pH. This technology

also reduces excessive hydrophobic interactions between the stationary phase and the compounds, and yet maintains great interfacial kinet-

ics for high efficiency.

Characteristics: Metal Impurity < 30 ppm; Pore Size 150 Å; Specific Surface Area 240 m2/g; Carbon Loading 15%; Double End-cap; Available

Particle Size: 5µm, 10 µm

Main Features:

●Wide pH range (1.5-12)

●Minimal silanol activity

●Better efficiency than many other high pH compatible columns

●High loading capacity for basic compounds for preparative applications

High pH Stability

Column: Durashell RP; 4.6 x 150 mm

Mobile Phase: 50% ACN/50% 0.05M Ammonia (pH=9.0)

Flow rate: 1.5 mL/min

Sample: doxepin, nortriptyline, amitriptyline,

trimipramine ,

Temperature: 35oC


NEW in HPLC !

Initial

After 100 h

0.70

0.60

0.50

0.40

0.30

0.20

0.10

0.00

-0.10

2.00 4.00 6.00 8.00 10.00 12.00 14.00 18.00

HP

LC C

olum

ns

016



Type Particle size (µm) Dimension (mm) Durashell RP

Surface Area: 240m2/g, Pore Size: 150Å

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Guard cartridge

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Guard cartridge

Semi-preparative

Semi-preparative

Preparative

Preparative

Preparative

Preparative

Preparative

Preparative

Guard cartridge

Semi-preparative

Semi-preparative

Preparative

Preparative

Preparative

Preparative

Preparative

Preparative

Preparative

Preparative

Guard cartridge

5

5

5

5

5

5

5

5

5

5

3

3

3

3

3

3

3

3

5

5

5

5

5

5

5

5

5

10

10

10

10

10

10

10

10

10

10

10

DS950302-0

DS950502-0

DS951002-0

DS951502-0

DS950505-0

DS951005-0

DS951505-0

DS952005-0

DS952505-0

DS950105-0

DS930302-0

DS930502-0

DS931002-0

DS931502-0

DS930505-0

DS931005-0

DS931505-0

DS930105-0

DS951510-0

DS952510-0

DS950520-0

DS951520-0

DS952520-0

DS951030-0

DS951530-0

DS952530-0

DS950220-0

DS901510-0

DS902510-0

DS900520-0

DS901520-0

DS902520-0

DS901030-0

DS901530-0

DS902530-0

DS901550-0

DS902550-0

DS900220-0

2.1×30

2.1×50

2.1×100

2.1×150

4.6×50

4.6×100

4.6×150

4.6×200

4.6×250

4.6×10

2.1×30

2.1×50

2.1×100

2.1×150

4.6×50

4.6×100

4.6×150

4.6×10

10×150

10×250

20×50

20×150

20×250

30×100

30×150

30×250

20×20

10×150

10×250

20×50

20×150

20×250

30×100

30×150

30×250

50×150

50×250

20×20

HP

LC C

olum

ns

017


Venusil Family of HPLC Columns Full Line of High Quality Products

Introduction of Venusil HPLC Columns

Venusil HPLC columns are manufactured from the highest purity spherical silica particles on the market. The outstanding properties of the

HPLC phases are results of subjecting high quality silica to Agela’ s innovative surface modification and unique bonding processes. Our

proprietary nanosurface modification generates a very smooth and even surface. This reduces the interaction between the silica surface and

polar compounds, resulting in symmetric peak shape even for very basic compounds. Based on this technology, a series of unique columns

were developed to meet the tough requirements for the analysis of highly polar compounds. Lot-to-lot reproducibility is ensured by a stable

bonding/end-capping process. The uniform, spherical particles have a nominal surface area of 380m2/g, 200 m2/g or 45m2/g, with a controlled

pore size of 100Å, 150Å or 300Å, respectively. All columns are packed using a consistent slurry packing process to achieve uniform and

stable beds for maximum column efficiency, lifetime and column-to-column reproducibility.

Unprecedented Experience in Chromatography

You will experience unprecedented performance using Venusil columns for your HPLC separations

Best Peak Symmetry and Efficiency

The Venusil columns generate symmetric peaks with

high efficiency over their entire applicable pH range,

outperforming competitors columns.

The pH-independent high performance feature of

Venusil columns allows scientists to establish rugged

methods with flexible choice of pH.

Tightest Specification

All our columns have tighter specification (see the table) than other

brand-name columns. This ensures that all columns have great

performance and identical selectivity

Comparison of Specifications

Waters Symmetry-C18

4.6×150mm, 5µm

Zorbax XDB-C18

4.6×150mm ,5µm

Venusil XBP C18 (2)

4.6×150mm ,5µm

N/A

N/A

1.8

+/ 10%

2 8

300

16%

Spec. on efficiency

Spec. on tailing factor(toluene)

Spec. on tailing factor amitriptyline

Spec. on selectivity(amitriptyline/acenaphthene)

pH range

Specific Surface

Carbon%

8000

0.98 1.25

N/A(typically 1.5 2.0)

N/A

2 9

180

12%

12000

0.98 1.12

1.4

+/ 7%

1.5 9

380

19%

Characteristics of Venusil Columns Benefit to the Columns’ users

Perfect peak symmetry, independent of pH

and mobile phase

Broad pH range (1 10.5)

Compatible with 100% aqueous mobile phase

Highest surface coverage

A wide variety of chemistries

Minimum buffer concentration needed

Tightest specification

Excellent column efficiency

Great column lifetime

Highest flexibility for any application,better sensitivity,

greater confidence on HPLC results

Flexibility for method development

Maximum versatility

Tolerance of large volume and mass injection

Broad selection of polarity and maximum versatility

Better sensitivity for LC/MS, extended column lifetime

Better reproducibility, greater confidence on the HPLC results

Better and faster separation

Lower cost; greater convenience


-

HP

LC

Colu

mns

018


Expanding the Capabilities with the Venusil Family of C18 Columns

Many of our columns have very unique properties to address some special needs. Here are some examples:

Venusil XBP-C18 (2): a phase designed for balanced adsorption to avoid excessive retention of hydrophobic compounds,

• Great peak symmetry for all types of compounds,

• Improved separation of stereo isomers

• Extremely narrow specification during manufacture to offer high column-to-column reproducibility

• Non-excessive retention for hydrophobic peaks (less peak broadening of later eluting compounds compared to

other columns)

Venusil ASB C18: a phase designed for low pH, low bleed (high sensitivity for LC-MS) and strong separation power for polar

compounds:

• Extremely low pH stability: pH limit =0.8 at 70

• Extremely low bleed offering high sensitivity for LC-MS under acidic conditions

• Compatible with 100% aqueous to 100% organic mobile phases

• Non-endcapped but with low surface acidity/activity compared to other non-endcapped stationary phases

Venusil AQ C18: a phase designed for polar and semi-polar compounds, to be compatible with 100% water :

• Compatible with 100% aqueous to 100% organic mobile phases

• Applicable to a variety of analytes: from very polar to non-polar

• Operates over a wide pH range: 1.0-9.0

• Applicable to a wide range of sample types: plasma, urine, drug formulation, food extraction

• Available in a range of column diameters suitable for LC-MS, conventional analytical, and preparative scale

Venusil XBP-C18: a phase designed for maximum hydrophobicity and high pH tolerance:

• The highest carbon loading and the most hydrophobic column on the market.

• High pH tolerance

• Not suggested for samples containing highly hydrophobic compounds

Venusil XBP-C18 (L): a phase designed for larger molecules and highly hydrophobic compounds:

• Larger pore size and lower surface area

• Accelerated elution for highly hydrophobic compounds

• Easier column clean-up for samples containing hydrophobic impurities or samples extracted by non-polar solvents

• Better choice for molecules > 500 Dalton

Agela Technologies has developed a series of high quality C18 stationary phases to meet a wide range of application needs.These columns

contain an ultra pure silica subjected to our patented surface deactivation process. By altering the column chemistry, we are able to tune the

surface properties of the silica particles and alter selectivity to meet a variety of application needs.

HP

LC C

olum

ns

019



Hydrophobicity Test

Inertness Test

Venusil XBP C18 (2) Series Columns

Universal, Highly Inert C18 Columns

• Balanced hydrophobic adsorption which results in a broader

suitability

• Stronger separation power for isomers

• Great column-to-column and batch-to-batch reproducibility

• Perfect symmetry for basic, acidic and neutral compounds

A good replacement of Luna C18 (2) columns!

Technical Parameters

• Metal Impuriy < 10ppm

• Particle Size: 5μm, 3μm, 10μm

• Specific Surface: 380m2/g

• Pore Size: 100Å

• Carbon Loading: 19%

• Double End-cap

• pH Range: 1.5-9.0

• Performance: Efficiency 80,000/m (5μm)

• TF: 0.98-1.12

Column: Venusil XBP C18 (2), 4.6 × 150mm, 5 μm


Mobile phase: MeOH: 25 mM KH2PO

4/K

2HPO

4 (pH 6.0) = 80 : 20


Flow rate: 1mL/min

Venusil XBP C18 (2) column packing material is made with ultra pure silica. The silica surface is processed with Agela’ s patented surface

deactivation technology, followed by a unique bonding process that can reduce the carbon content while maintaining a high bonding cover-

age. The Venusil XBP C18 column does not have excessive retention for highly hydrophobic compounds, and it is great for the separation

of acidic, basic, and neutral compounds. Moreover, this column also has superb resolution power for isomers. The perfect peak symmetry

offered by this RP column makes it a great first-choice for HPLC method development.

Column: Venusil XBP C18 (2), 4.6 × 150mm, 5 μm

Sample: Amitriptyline

Mobile phase: NaH2PO

4/Na

2HPO

4 20mM, pH 6.9 :

MeOH = 29 : 71


Flow rate: 1mL/min

Amitriptyline is a strong basic antidepressant. It is recognized as

a compound difficult to obtain good peak shape;

But the XBP C18 2 column performed outstandingly and gave

a great peak symmetry TF = 1.12 .

min0 1 2 3 4 5

mAU

0

20

40

60

80

100

120

1.6

81

4.7

11

HP

LC C

olum

ns

020


Selectivity of Basic Compounds

Venusil XBP C18(2),4.6×150mm,5 μm

Part No.: VX951505-2

Brand Z C18 column 4.6×150mm,5 μm

Brand H C18 column 4.6×150mm,5 μm

Mobile Phase: MeOH Acetic Acid/ sodium acetate aq.

(pH 5.0 =60 40

Flow Rate 1 mL/min

Temperature 30

Detection UV 254 nm

Sample p-Methyl benzoic acid, Pyridine,Phenol,

Acetophenone, Amino-dimethyl

benzene, Toluene

Extremely Low Metal Effects

Mobile Phase: Water :Methanol=65:35

Flow Rate: 1 mL/min

Temperature: 35


Sample: 2,3-Dihydroxynaphthalene 2,7

-Dihydroxynaphthalene

Venusil XBP C18(2) 4.6×150mm

Part.No.:VX951505-2

Brand Y C18 4.6×150mm

Brand H 4.6×150mm


Surface Area: 380m2/g,Pore Size: 100Å

Type Dimension (mm) Particle size(µm) Venusil XBP-C18 (2)

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Guard cartridge

2.1×30

2.1×50

2.1×100

2.1×150

4.6×50

4.6×100

4.6×150

4.6×250

4.6×10 4/pk

5

5

5

5

5

5

5

5

5

VX950302-2

VX950502-2

VX951002-2

VX951502-2

VX950505-2

VX951005-2

VX951505-2

VX952505-2

VX950105-2

HP

LC C

olum

ns

021


Comparison of Venusil-C8 Phases

Plates (Toluene) Tailing Factor

3805

2546

4012

Brand Z-C8

Luna C8

Venusil XBP-C8

1.12

1.15

1.03

Sample: uracil, phenol,chloronitrobenzene,

toluene(in methanol/water=50/50)

Column: 2.1 x 50 mm, 5 μm

Mobile phase: methanol: water = 65: 35


Temperature: 30 oC

Sample: Uracil, Doxepin, Nortriptyline,Amitriptyline,

Trimipramine

Column: Venusil XBP-C18, 4.6 x 150 mm, 5 μm

Mobile phase: 0.01 M sodium phosphate: ACN= 50 : 50, pH=7.6


Temperature: 45 oC

Venusil XBP Series Columns

Venusil XBP C18

Venusil XBP C18 columns have the maximum bonding

density therefore the highest hydrophobicity

(the lowest polarity). This allows for the least interaction

between the analytes and the silanol groups. Venusil XBP

columns have extraordinary column stability at high pHs.

Pore size: 100Å, 300 Å; Specific surface: 380 m2/g,

45m2/g; Purity > 99.999%;

1) Inertness: one of most base-friendly columns.

2) Efficiency: excellent at any applicable pH.

3) Large volume injection: maintain very high efficiency with

very large injection volumes.

4) Broad pH range (1.5 – 10.0 ): low pH, 0.2 % TFA; high pH,

0.010 M triethylamine.

Other Venusil XBP Columns: A complete line of stationary

phases include C8, C4, C1, NH2, Phenyl, CN, Silica, SCX,

SAX;

Quality of Column Packing Perfect Peak Symmetry and Great Lifetime of

the Column for Basic Compounds at Mid-pH


0.6

73

1.0

31

2.0

16

2.9

04

Brand Z C8

0.7

41

1.1

87

2.7

37

3.8

32

Venusil XBP C8

0.7

38

1.1

90

2.4

07

3.4

59

Luna C8

Initial

After 10 days

0.6

07

0.7

01

0.6

18 0.8

58

3.8

22

6.0

47

10.2

57

17.0

21

0.7

27

0.8

55

3.3

84

5.8

10

9.7

76

16.7

55

HP

LC C

olum

ns

022




Type Dimension(mm) Particle(μm) Venusil XBP-C18 Venusil XBP-C8 Venusil XBP-C4 Venusil XBP-C1

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Guard cartridge

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Guard cartridge

Semi-preparative

Semi-preparative

Preparative

Preparative

Preparative

Preparative

Preparative

Preparative

Guard cartridge

Semi-preparative

Semi-preparative

Preparative

Preparative

Preparative

Preparative

Preparative

Preparative

Preparative

Preparative

Guard cartridge

2.1×30

2.1×50

2.1×100

2.1×150

4.6×50

4.6×100

4.6×150

4.6×200

4.6×250

4.6×10 4/pk

2.1×30

2.1×50

2.1×100

2.1×150

4.6×50

4.6×100

4.6×150

4.6×10 4/pk

10×150

10×250

20×50

20×150

20×250

30×100

30×150

30×250

20×10

10×150

10×250

20×50

20×150

20×250

30×100

30×150

30×250

50×150

50×250

20×10

5

5

5

5

5

5

5

5

5

5

3

3

3

3

3

3

3

3

5

5

5

5

5

5

5

5

5

10

10

10

10

10

10

10

10

10

10

10

VX950302-0

VX950502-0

VX951002-0

VX951502-0

VX950505-0

VX951005-0

VX951505-0

VX952005-0

VX952505-0

VX950105-0

VX930302-0

VX930502-0

VX931002-0

VX931502-0

VX930505-0

VX931005-0

VX931505-0

VX930105-0

VX951510-0

VX952510-0

VX950520-0

VX951520-0

VX952520-0

VX951030-0

VX951530-0

VX952530-0

VX950120-0

VX901510-0

VX902510-0

VX900520-0

VX901520-0

VX902520-0

VX901030-0

VX901530-0

VX902530-0

VX901550-0

VX902550-0

VX900120-0

VX450302-0

VX450502-0

VX451002-0

VX451502-0

VX450505-0

VX451005-0

VX451505-0

VX452005-0

VX452505-0

VX450105-0

VX150302-0

VX150502-0

VX151002-0

VX151502-0

VX150505-0

VX151005-0

VX151505-0

VX152005-0

VX152505-0

VX150105-0

VX850302-0

VX850502-0

VX851002-0

VX851502-0

VX850505-0

VX851005-0

VX851505-0

VX852005-0

VX852505-0

VX850105-0

VX830302-0

VX830502-0

VX831002-0

VX831502-0

VX830505-0

VX831005-0

VX831505-0

VX830105-0

VX851510-0

VX852510-0

VX850520-0

VX851520-0

VX852520-0

VX851030-0

VX851530-0

VX852530-0

VX850120-0

VX801510-0

VX802510-0

VX800520-0

VX801520-0

VX802520-0

VX801030-0

VX801530-0

VX802530-0

VX801550-0

VX802550-0

VX800120-0


Dimension(mm)

Particle(μm)

VenusilXBP-NH

2

VenusilXBP-Phenyl

VenusilXBP-CN

VenusilSilica

Type

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Guard cartridge

2.1×30

2.1×50

2.1×100

2.1×150

4.6×50

4.6×100

4.6×150

4.6×200

4.6×250

4.6×10 4/pk

5

5

5

5

5

5

5

5

5

5

VN850302-0

VN850502-0

VN851002-0

VN851502-0

VN850505-0

VN851005-0

VN851505-0

VN852005-0

VN852505-0

VN850105-0

VC950302-0

VC950502-0

VC951002-0

VC951502-0

VC950505-0

VC951005-0

VC951505-0

VC952005-0

VC952505-0

VC950105-0

VSi950302-0

VSi950502-0

VSi951002-0

VSi951502-0

VSi950505-0

VSi951005-0

VSi951505-0

VSi952005-0

VSi952505-0

VSi950105-0

VX650302-0

VX650502-0

VX651002-0

VX651502-0

VX650505-0

VX651005-0

VX651505-0

VX652005-0

VX652505-0

VX650105-0

HP

LC C

olum

ns

023



Dimension(mm)

Particle(μm)

VenusilXBP-C18

VenusilXBP-C8

VenusilXBP-C4

VenusilXBP-C1

Type

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Guard cartridge

Semi-preparative

Semi-preparative

Preparative

Preparative

Preparative

Guard cartridge

2.1×30

2.1×50

2.1×100

2.1×150

4.6×50

4.6×100

4.6×150

4.6×200

4.6×250

4.6×10 4/pk

10×150

10×250

20×50

20×150

20×250

20×10

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

VX950302-T

VX950502-T

VX951002-T

VX951502-T

VX950505-T

VX951005-T

VX951505-T

VX952005-T

VX952505-T

VX950105-T

VX951510-T

VX952510-T

VX950520-T

VX951520-T

VX952520-T

VX950120-T

VX450302-T

VX450502-T

VX451002-T

VX451502-T

VX450505-T

VX451005-T

VX451505-T

VX452005-T

VX452505-T

VX450105-T

VX150302-T

VX150502-T

VX151002-T

VX151502-T

VX150505-T

VX151005-T

VX151505-T

VX152005-T

VX152505-T

VX150105-T

VX850302-T

VX850502-T

VX851002-T

VX851502-T

VX850505-T

VX851005-T

VX851505-T

VX852005-T

VX852505-T

VX850105-T

VX851510-T

VX852510-T

VX850520-T

VX851520-T

VX852520-T

VX850120-T


Dimension(mm)

Particle(μm)

VenusilXBP-NH

2

VenusilXBP-Phenyl

VenusilXBP-CN

VenusilSilica

Type

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Guard cartridge

2.1×30

2.1×50

2.1×100

2.1×150

4.6×50

4.6×100

4.6×150

4.6×200

4.6×250

4.6×10 4/pk

5

5

5

5

5

5

5

5

5

5

VN850302-T

VN850502-T

VN851002-T

VN851502-T

VN850505-T

VN851005-T

VN851505-T

VN852005-T

VN852505-T

VN850105-T

VC950302-T

VC950502-T

VC951002-T

VC951502-T

VC950505-T

VC951005-T

VC951505-T

VC952005-T

VC952505-T

VC950105-T

VSi950302-T

VSi950502-T

VSi951002-T

VSi951502-T

VSi950505-T

VSi951005-T

VSi951505-T

VSi952005-T

VSi952505-T

VSi950105-T

VX650302-T

VX650502-T

VX651002-T

VX651502-T

VX650505-T

VX651005-T

VX651505-T

VX652005-T

VX652505-T

VX650105-T


HP

LC C

olum

ns

024


Venusil XBP (L) Series Columns


Dimension(mm)

Particle(μm)

Type

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

2.1×30

2.1×50

2.1×100

2.1×150

4.6×50

4.6×100

4.6×150

4.6×250

5

5

5

5

5

5

5

5

VenusilXBP(L) C18

VX950302-L

VX950502-L

VX951002-L

VX951502-L

VX950505-L

VX951005-L

VX951505-L

VX952505-L

VenusilXBP(L) Silica

VSi950302-L

VSi950502-L

VSi951002-L

VSi951502-L

VSi950505-L

VSi951005-L

VSi951505-L

VSi952505-L

VenusilXBP(L) C8

VX850302-L

VX850502-L

VX851002-L

VX851502-L

VX850505-L

VX851005-L

VX851505-L

VX852505-L

XBP (L) C18 has an identical bonded phase as XBP C18. However, XBP (L) C18 has relatively low surface area which allows

compounds to have shorter retention times. These columns are an excellent alternative to Hypersil BDS-C18, Hypersil BDS-C8;

Zorbax XDB-C18, Zorbax XDB-C8 which comparitively could have too much retention.

XBP (L) C18 have relatively larger pore size (150Å) and are suitable for the separation of large molecules.

StationaryPhase

Venusil

XBP-C18(2)

Venusil

XBP-C18

Venusil

XBP-C18(L)

Venusil

AQ C18

Venusil

ASB-C18

Specific SurfaceArea (m2/g)

Pore Size(Å)

CarbonLoading

Particle SizeAvailable (μm)

pHRange

Characteristics

380

380

200

380

200

100

100

150

100

150

19%

22%

15%

18%

12%

3, 5, 10

3, 5, 10

3, 5, 10

3, 5, 10

3, 5, 10

1.5

1.5

1.5

1.0

0.8

Balanced adsorption to avoid excessive reten-tion; a wider range of suitability; perfect peak symmetry for acidic, basic and neutral com-pounds; greater separation power for isomers.

The most hydrophobic adsorption; better high pH tolerance; not suggested for samples which contain highly hydrophobic compounds.

Larger pore size and lower surface area;a better choice for the analysis of large molecule and strong hydrophobic compounds.

Slightly polar C18 and enhanced retention for polar compounds; 100% water compatible; good peak shape for acidic, basic and neutral compounds.

Polar C18; extremely low bleed at low pH; great for LC-MS and peptide separation; stable at pH as low as 0.8 at 70 .

HP

LC C

olum

ns

025


Venusil AQ-C18 Columns

The Venusil AQ-C18 column is designed for the separation of polar, semi-polar and non-polar compounds at low to medium pH. This column

is more polar than XBP-C18, but less polar than ASB-C18. With a special surface treatment, Venusil AQ-C18 is made to be compatible with

100% aqueous mobile phases., and it can be used to replace Waters Symmetry, Inertsil C18, Zorbax SB, Atlantis dC18, and other AQ

columns. This column has a pH range of 1.5-9.0.

Pore size: 100 Å; Specific surface: 380 m2/g; Purity > 99.999%;

Compatibility with 100% Aqueous Mobile Phase

Sample: Ranitidine

Column: 4.6×150mm,5μm

Mobile phase: 100% Water

Flow rate: 1mL/min; the flow was stopped for 5 minutes

during the testing period for each column.

Temperature: 30

4) 100% water compatible: very unique for a universal C18

column; much better peak shape, retention, and efficiency

than other brand AQ columns.

5) Low pH stability (pH=1.5):better stability than most of the

popular brand columns (including other brand AQ columns)

on the market.

1) Inertness: one of most base-friendly columns, excellent

peak symmetry of basic compounds.

2) Efficiency: The best column for polar compounds at any pH.

3) Large volume injection: maintains very high efficiency even

if the injection volume is exceptionally large.


HP

LC

Colu

mns

026


Balanced Retention for Hydrophilic and Hydrophobic Compounds

Mobile Phase: 35% v/v 20.0 mM KH2PO

4/K

2HPO

4

at pH7.00/65% Methanol at 23

Flow: 1mL/min

Detection: UV 254nm

Sample: Uracil Propranolol hydrochloride

Butyl ,Phthalate Dimethyl Phthalate

Naphthalene ,Acenaphthene Amitriptyline

Mobile Phase 1% Acetic Acid (pH=2.57) :MeOH =50:50

Flow 1mL/min

Temperature 30

Detection 275nm

Sample Uracil Paracetamol acetophenone and

2-Hydroxybenzoic acid

Great Peak Shape for All Type of Compounds

min0 2 4 6 8 10 12 14 16 18

mAU

0

100

200

300

400

VWD1 A, Wavelength=275 nm (HLX\08021218.D)

2.8

09

3.5

44

10

.88

4

11

.77

0

Venusil AQ Column,4.6×250mm

Part No.: VA952505-0

VWD1 A, Wavelength=275 nm (HLX\08021217.D)

2.9

44

3.5

21

9.4

85

11

.41

3

Brand Y AQ Column,4.6×250mm

mAU

0

100

200

300

400

min0 2 4 6 8 10 12 14 16 18

Venusil AQ ,4.6×100mm,


Brand Y AQ ,4.6×100mm

HP

LC C

olum

ns

027




Dimension(mm)

Particle(μm)

VenusilAQ-C18

Type

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Guard cartridge

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Guard cartridge

Semi-preparative

Semi-preparative

Preparative

Preparative

Preparative

Preparative

Preparative

Preparative

Guard cartridge

2.1 30

2.1 50

2.1 100

2.1 150

4.6 50

4.6 100

4.6 150

4.6 250

4.6 10 4/pk

2.1 30

2.1 50

2.1 100

2.1 150

4.6 50

4.6 100

4.6 150

4.6 10 4/pk

10 150

10 250

20 50

20 150

20 250

30 100

30 150

30 250

20 10

5

5

5

5

5

5

5

5

5

3

3

3

3

3

3

3

3

5

5

5

5

5

5

5

5

5

VA950302-0

VA950502-0

VA951002-0

VA951502-0

VA950505-0

VA951005-0

VA951505-0

VA952505-0

VA950105-0

VA930302-3

VA930502-0

VA931002-0

VA931502-0

VA930505-0

VA931005-0

VA931505-0

VA930105-0

VA951510-0

VA952510-0

VA950520-0

VA951520-0

VA952520-0

VA951030-0

VA951530-0

VA952530-0

VA950120-0


HP

LC C

olum

ns

028


The Venusil ASB column is specially designed for the separation of polar compounds from low (extremely stable at pH=1) to medium pH. The

stationary phase is end-capped with polar groups and thus has the highest polarity among all Venusil family columns. This column can be

used to replace Water Atlantis, Zorbax SB, and other brand AQ columns.

Pore size: 150Å; Specific surface: 200m2/g; Purity 99.999%

min

Column: Venusil ASB C18, 5μm, 4.6×150mm

Sample: Naphthol

Aging: 40 , TFA in 80% methanol (pH=1.0), 400 hours

Mobile Phase:TFA in 80% methanol (pH=1.5)

Flow Rate:1 mL/min

Injection: 5 μL

Temperature:30

Venusil ASB Columns

1) Low pH stability: great stability even at pH=1

2) Peak shape and efficiency: Excellent peak symmetry

for basic compounds comparing to other brand polar

phase columns

3) Polar C18 phase: Very strong separation power for

polar compounds

4) 100% aqueous compatible: Much better peak shape,

retention, and efficiency comparing to other brand “AQ”

columns.

The Stability in the Low pH

The separation of Organic Acid

Column: Venusil ASB C18, 5μm, 4.6×150mm

Sample: Vc, malonic acid, lactic acid, citric acid

Mobile Phase:20 mM Phosphate buffer saline (PBS) , pH=2.0

Flow Rate:1 mL/min

Injection: 5 μL

Detection:UV 210 nm

0

0 1 2 3 4 5 6 7 8 9

10

20

30

40

50

mAU

0

20

40

60

80

100

140

120

mAU

min

0 1 2 3 4 5 6 7 8 9 min

0 1 2 3 4 5 6 7 8

0

5

10

15

20

25

30

40

35

mAU

VWD1A,Wavelength=210nm(ZZX\0503-046.D)

Area:21.2573

HP

LC C

olum

ns

029



Surface: 200m2/g,Pore size: 150Å

Dimension(mm)

Particle(μm)

VenusilASB-C18

Type

Type

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Guard cartridge

Semi-preparative

Semi-preparative

Preparative

Preparative

Preparative

Guard cartridge

2.1 30

2.1 50

2.1 100

2.1 150

4.6 50

4.6 100

4.6 150

4.6 250

4.6 10 4/pk

10 150

10 250

20 50

20 150

20 250

20 10

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

VS950302-0

VS950502-0

VS951002-0

VS951502-0

VS950505-0

VS951005-0

VS951505-0

VS952505-0

VS950105-0

VS951510-0

VS952510-0

VS950520-0

VS951520-0

VS952520-0

VS950120-0

VenusilASB-C8

VS850302-0

VS850502-0

VS851002-0

VS851502-0

VS950505-0

VS851005-0

VS851505-0

VS852505-0

VS850105-0

VS851510-0

VS852510-0

VS850520-0

VS851520-0

VS852520-0

VS850120-0

VenusilASB-Phenyl

VS650302-0

VS650502-0

VS651002-0

VS651502-0

VS650505-0

VS651005-0

VS651505-0

VS652505-0

VS650105-0

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytica

Guard cartridge

2.1 30

2.1 50

2.1 100

2.1 150

4.6 50

4.6 100

4.6 150

4.6 250

4.6 10 4/pk

5

5

5

5

5

5

5

5

5

VS950302-T

VS950502-T

VS951002-T

VS951502-T

VS950505-T

VS951005-T

VS951505-T

VS952505-T

VS950105-T

VS850302-T

VS850502-T

VS851002-T

VS851502-T

VS850505-T

VS851005-T

VS851505-T

VS852505-T

VS850105-T

Surface Aera: 45m2/g,Pore Size: 300Å

Dimension(mm)

Particle(μm)

VenusilASB-C18

VenusilASB-C8


HP

LC C

olum

ns

030


Venusil SCX HPLC Columns

SCX HPLC Column: made of spherical silica particles of the highest purity (>99.999%), bonded with aromatic sulfonic acid

group.They can be used forthe separation of basic, water-soluble compounds and bio-molecules.


Dimension(mm)

Particle(μm)

VenusilSCX

Type

Analytical

Analytical

Analytical

Guard cartridge

4.6×100

4.6×150

4.6×250

4.6×10 4/pk

5

5

5

5

VSc951005-0

VSc951505-0

VSc952505-0

VSc950105-0



Dimension

(mm)

Particle

(μm)

Venusil

SCX Type

Analytical

Analytical

Analytical

Guard cartridge

4.6×100

4.6×150

4.6×250

4.6×10 4/pk

5

5

5

5

VSc951005-T

VSc951505-T

VSc952505-T

VSc950105-T

Venusil SAX HPLC Columns

SAX HPLC Column: the stationary phase is made with high purity spherical silica particles and bonded with quaternary amine groups.

The column can be used for the separation of acidic, water-soluble compounds and bio-molecules


Dimension(mm)

Particle(μm)

VenusilSAX

Type

Analytical

Analytical

Analytical

Guard cartridge

4.6×100

4.6×150

4.6×250

4.6×10 4/pk

5

5

5

5

VSa951005-0

VSa951505-0

VSa952505-0

VSa950105-0



Dimension

(mm)

Particle

(μm)

Venusil

SAX Type

Analytical

Analytical

Analytical

Guard cartridge

4.6×100

4.6×150

4.6×250

4.6×10 4/pk

5

5

5

5

VSa951005-T

VSa951505-T

VSa952505-T

VSa950105-T

HP

LC C

olum

ns

031


Promosil HPLC Columns

• Double End-cap, Good Inertness

• Good Stability and Reproducibility

• Excellent Performance-to-cost Value

Using the silica of high-purity and high mechanical strength, the Promosil C18s are made with high-purity monosilane through Agela

Technologies’ well controlled bonding process. They have high surface bonding coverage and completely capped. The carbon content

is as much as 18%. They are stable at pH range 1.5-9.0 showing good peak shape for acidic and basic compounds. They have excellent

tolerance of contamination and long life-time. They are the best choice of high performance-to-cost value.

Technical Parameters:

• High-purity silica, Metal Impurity<10ppm

• Average Particle Size: 5μm

• Specific Surface: 320m2/g

• Pore Size: 100Å

• Carbon Loading: 18%

• pH Range: 1.5-9.0

• Efficiency: >80, 000/m

• TF: 0.98-1.20

Hydrophobicity Test

Column: Promosil C18, 4.6 × 150mm, 5 μm


Mobile phase: MeOH: 25 mM KH2PO

4/K

2HPO

4 ( pH 6.0 ) = 80 : 20


Flow rate: 1mL/min min0 1 2 3 4 5

mAU

0

20

40

60

80

100

120

140

1.4

96

4.3

48

Inertness Test

Column: Promosil C18, 4.6 × 150mm, 5 μm

Sample: Amitriptyline

Mobile phase: NaH2PO

4/Na

2HPO

4 (20mM, pH 6.9): MeOH = 32 : 68


Flow rate: 1mL/min


mAU

-100

-50

0

50

100

150

200

1.6

67 5

.44

0

2.9

23

1.8

70

2.4

28

7.3

35

7.8

09

9.8

78

19

.97

4

34

.15

5

HP

LC C

olum

ns

032


Other Manufacturers’ Brands

Agela Technologies may also supply a number of major brand name HPLC columns made by other manufacturers, as listed below. Please

contact us for availability and price information..

These brands are: Zobax, Kromasil, Hypersil, ES-Industry, Shodex, Jordi


Dimension(mm)

2.1 30

2.1 50

2.1 100

2.1 150

4.6 50

4.6 100

4.6 150

4.6 250

4.6 10, 4/pk

Particle(μm)

5

5

5

5

5

5

5

5

5

PromosilC18

PM950302-0

PM950502-0

PM951002-0

PM951502-0

PM950505-0

PM951005-0

PM951505-0

PM952505-0

PM950105-0

PromosilC8

PM850302-0

PM850502-0

PM851002-0

PM851502-0

PM850505-0

PM851005-0

PM851505-0

PM852505-0

PM850105-0

PromosilNH

2

PM750302-0

PM750502-0

PM751002-0

PM751502-0

PM750505-0

PM751005-0

PM751505-0

PM752505-0

PM750105-0

PromosilCN

PM550302-0

PM550502-0

PM551002-0

PM551502-0

PM550505-0

PM551005-0

PM551505-0

PM552505-0

PM550105-0

PromosilSilica

PM050302-0

PM050502-0

PM051002-0

PM051502-0

PM050505-0

PM051005-0

PM051505-0

PM052505-0

PM050105-0

Surface area: 320m2/g Pore size: 100Å

HP

LC

Colu

mns

033

Best Value




Solutions for Highly Water Soluble Compounds:

Venusil AQ C18

Venusil ASB C18

Unisol Amide

It is known that highly water-soluble compounds often have difficulties in HPLC analysis, such as lack of retention, bad peak shape and inconsistent

results. A large scientific effort has been made in developing HPLC stationary phases that can provide solutions to these problems. However, no

single stationary phase may resolve all these difficulties. Agela Technologies has developed a series of stationary phases along with development

schemes to help chemists systematically develop methods for the analysis of highly water-soluble compounds. The series includes two reverse

stationary phases which are compatible with 100% water and one strong hydrophilic interaction stationary phase which has a neutral functionality on

its surface. Some application examples are included as a guide to your column selection.

Venusil AQ-C18 is a slightly-polar C18 column, and it is our most versatile high aqueous reverse phase column. Venusil AQ-C18 has a broad pH

suitability (1.5-9.0) and thus can be your first option for most of your HPLC applications. Venusil ASB-C18 has pH range of 0.8-7.0. The column

stationary phase is non-end capped polar C18 and good for low pH conditions. Unisol Amide (HILIC) has a pH range of 2-8 . Unisol Amide column

stationary phase is modified with strong hydrophilic and neutral functionalities, it has hydrophilic interaction with the compounds. Unisol Amide

provides the strongest retention to hydrophilic compounds among these three types of columns.

In general, your method development may start with a Venusil AQ-C18 column and a mobile phase containing a mixture of methanol or acetonitrile

with an aqueous acidic buffer solution (pH=2-5). This approach may be applicable to the HPLC analysis of more than 50% of small molecules

(<2000 daltons). If required, you can minimize the percentage of the stronger organic mobile phase to increase retention by using up to 100% aque-

ous acidic buffer mobile phase. In the cases that you achieve adequate retention, but not enough resolution for the compounds of your interest, you

may chose to adjust mobile phase ratio, mobile phase pH (very effective for ionizable compounds), or chose to replace the column with a Venusil

ASB-C18 or Unisol Amide for an alternative selectivity. Figure 1 shows an HPLC separation of four organic acids using Unisol C18 column in 100%

aqueous mobile phase at pH=2.

In the cases that you cannot achieve adequate retention after analysis in 100% aqueous mobile phase using the Venusil AQ-C18, you have the

following options depending on the type of compounds. For acidic compounds, you may try ASB-C18 columns and lower the pH to further reduce

the solubility of the compounds in water. For such low pH applications, TFA is the preferred acidic modifier. TFA may give you the highest retention

of both acidic and basic compounds than other organic or inorganic acidic modifiers. If necessary, you may also try a Unisol Amide column in HILIC

mode for even higher retention and/or different selectivity. When using a Unisol Amide column, it will behave like a NP column. More polar com-

pounds will elute later than less polar compounds. Figure 2 shows a good separation of highly water-soluble shikimic acid and related substances

using a Unisol Amide column. The retention of these compounds was not adequate for analysis on either an AQ-C18 or an ASB-C18 column.

For basic compounds, you may start with a AQ- C18 column. For compounds with pKa > 9, another alternative is to use a Unisol Amide column in

place of the AQ- C18 column and operate at a low to medium pH (2-7). Figure 3 shows that even with a 100% aqueous mobile phase, validamycin

(basic and highly water soluable) still has poor retention on a AQ-C18 column. However, a Unisol Amide column operating at pH 2 in the hydrophilic

interaction mode resulted in a suitable validamycin separation.

For neutral compounds, changing pH will have a limited effect on retention. If a suitable method cannot be developed using an AQ-C18 column, an

ASB-C18 or Unisol Amide column may be required.

Usually during method development, the desired retention is achieved first, then the mobile phase pH and ratio is further adjusted to get the desired

resolution. When the desired retention or resolution cannot be obtained for highly water soluble compounds using a AQ-C18 or ASB-C18 columns, a

Unisol Amide column will be a good alternative. Unisol Amide columns offer completely different selectivity from reverse phase columns and offer

much higher retentivity .

The multiple selection of columns and this comprehensive method development scheme for the separation of highly water-soluble compounds will

allow you to develop HPLC methods at ease.



HP

LC

Colu

mns

034


Sample: Vitamin C, malonic acid, lactic acid, citric acid.

Column: AQ-C18, 4.6 x 150mm, 5 μm

Mobile phase 20mmol phosphate buffer pH=2.0

Detection UV 210nm


Temperature: 30oC

Column:Unisol Amide (HILIC) 4.6 x 250 mm, 5 μm

Mobile phase ACN/1% formic acid 90-60% in 20 min

Detection UV 210nm


Temperature: 30oC

Column:Unisol Amide (HILIC) 4.6 x 250 mm, 5 μm

Mobile phase ACN/0.1%TFA 85-40% in 30 min

Detection UV 210nm


Temperature: Ambient

Column:Waters Atlantis C18, 4.6 x 250 mm, 5 μm

Mobile phase TFA 1%


Figure 1. HPLC Separation of Organic Acids Figure 2. HPLC Analysis of Shikimic Acid

(3,4,5-Trihydroxy-1-cyclohexene-1-carboxylic Acid)

Figure 3. HPLC Analysis of Validamycin Raw Products

0 1 2 3 4 5 6 7 8 9

40

35

30

25

20

15

10

5

0

Area:

21.2

573

mA

U

min

2700

2200

1700

1200

700

200

2700

2200

1700

1200

700

2004

13

.17

0

12

.01

0

11

.39

7

10

.03

7

9.2

23

7.2

23

6.0

37

5.5

03

2.2

23

2.0

50

1.6

77

1.1

43

4.7

17

2

3.7

03

0 2 4 6 8 10 12 14m

AU

min

AU

2.00 4.00 6.00 8.00 10.00 12.00 14.00 16.00 18.00

0.12

0.10

0.08

0.06

0.04

0.02

0.00

0.02

min

0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50

2.00

1.80

1.60

1.40

1.20

1.00

0.80

0.60

0.40

0.20

0.00

-0.20

AU

min

HP

LC

Colu

mns

035

Best Value



Other Stationary Phases from Agela Technologies for Hydrophilic Compounds Separation:

Unisol C18

Venusil XBP-Silica (HILIC II)

Venusil XBP-NH2 (HILIC III)



Acidic,basic or neutral

compounds

Good Retention, But Not Enough Resolution

Not E nough R etention

- VenusilAQ-C18 Columns

- MeOH/water or ACN/water

- pH=2-6 (TFA, formic acid or other buffer)

- adjust mobile phase ratio or pH

- use Venusil ASB-C18 or Venusil HILIC

Acidic

Compounds

Basic

Compounds

Neutral

Compounds

adjust pH to 9-10; or use Venusil HILIC

use Venusil ASB-C18 and adjust pH to1-2 ; or use Venusil HILIC

use Venusil HILIC and adjust pH to 3-7

HP

LC

Colu

mns

036


Venusil ASB C18

min0 1 2 3 4 5 6 7

mAU

0

50

100

150

200

250

DAD1 A, Sig=240,4 Ref=360,100 (AGELA\07052506.D)

3.8

39

4.5

74

3.6

13

Column: Venusil ASB C18 4.6×250 mm 5 μm (VS952505-0)

Mobile Phase 100mM NH4AC:ACN=95:5

Detection 240 nm

Flow rate 1mL/min

Temperature 40

Sample: Melamine in Feed

Sample preparated by SPE

(Cleanert PCX 60mg/3mL; Part No.: CX0603)

Unisol C18

Column: Unisol C18; 4.6 × 150 mm

Mobile Phase: A: NH4AC Buffer (Adjust pH 6.0)

B: ACN

Gradient: 10% B to 60% B in 20 min.


Sample: Teicoplanin+Impurity

Temperature: 35

Teicoplanin

Nicousamide

Column: Unisol C18 4.6×150mm ( UO951505-0)

Mobile Phase: Water:ACN:THF (50:50:1,0.1%H3PO

4)

Flow Rate: 1mL/min

Sample: Nicousamide

Detection: UV 254nm

OH

OH

OH

OH

OH OH

HOOC

HOHO

HOHOHO

HO

HN

NH

NH

NH

NH

NH2

HN

HN

HO HO

H3C

CI

NHR

CI

H

H H

H

OO

OO

O

O

O

O

O

O

O

O

O

O

R

O

A2-1

A2-2

A2-3

A2-4

A2-5

(Z)-4-decenoic acid8-methylnonanoic aicd n-decanoic aicd8-methyldecanioc aicd 9-methyldecanioc aicd

0.0

5.00 10.00 15.00 20.00 25.00 30.00

1.0

2.0

3.0

4.0

5.0

6.0

7.0

8.0

9.0

Time

NO2

CH O

CH3

O

O

NH

COOH

OH

11.49

16332865

Teicoplanin

HP

LC

Colu

mns

037

Best Value



HILIC Column Family From Agela Technologies

Unisol Amide ( Agela HILIC )

Venusil XBP-Silica ( Agela HILIC II)

Venusil XBP-NH2 (Agela HILIC III)

Comparison:

●Selectivity

XBP-silica is slightly acidic (pH=5.6); while Unisol Amide is slightly basic (pH=7-9), and XBP-NH2 is more basic (pH=9.2).

XBP-silica has stronger retention of basic compounds, while Unisol Amide and XBP-NH2 have better retention of acidic compounds.

Unisol Amide has a balanced retention power for neutral, acidic and basic compounds, and thus the highest versatility.

●Reproducibility and Lifetime

Unisol Amide has the best reproducibility and lifetime because of a bonded and close-to-neutral protection layer.

Water-soluble Vitamins

●Selectivity comparison on 3 Columns

●Stability comparison of Unisol Amide and silica

Unisol Amide Column,

5μm, 4.6mm×150mm


XBP -NH2 Column,

5μm, 4.6×150mm

XBP-Silica Column,

5μm, 4.6×150mm

Mobile Phase: A: 0.1% TFA in Water

B: 0.1% TFA in Acetonitrile ; A:B =90:10

Flow Rate: 1 mL/min

Temperature: 30


Sample: VB1+VB6+VC+VB2



VB1 VB6

VB1

VC VB2

VB6

VC

VB2

VB1VB6

VCVB2

0 1 2 3 4 5 6

0 1 2 3 4 5 6

0 1 2 3 4 5 6

HP

LC

Colu

mns

038


Validamycin

OO

NH

CH2OH

HOH2C

OH

OH

CH2OH

OHHO

OH

OH

HO OH

Validamycin

Column: Unisol Amide,5μm, 4.6mm×250mm


Column:Brand W , AQ Column,5μm, 4.6mm×250mm

Mobile Phase: A: 0.1% TFA in Water

B: Acetonitrile


Flow Rate: 1 mL/min

Temperature: 25


Sample: Validamycin

Stability Comparison of Unisol Amide and Silica

Samples:VB1, VB6, VC, VB2

Aging Conditions:

MeOH:20 mM NaH2PO

4 (pH=7.00) = 40 : 60; 1.0mL/min Temp 40

HPLC Conditions:

Mobile Phase: 0.1%TFA : ACN=90:10

Detection: 280nm; Flow: 1.0 mL/min; Temp: 30?;Inj: 2 μL

Venusil HILIC, 4.6 × 150,5μm Brand W Silica, 4.6 × 150,5μm

Initial Initial

After agingAfter aging

VB1 VB2

VB6

VC

VB1 VB2

VB6

VC

VB1

VB2

VB6

VC

VB1

VB2

VB6

VC

HP

LC

Colu

mns

039

Best Value



Low pH Stability of ASB-C18

Column: Venusil ASB-C18, 4.6 ×150 mm, 5 μm

Sample: Naphthol

Aging: 40 , TFA in 80% methanol (pH=1.0), 400 hours

Mobile phase:TFA in 80% methanol (pH=1.5)


Injection: 5 μL

Temperature: 30

High pH Stability of Durashell RP

Initial

After 100 h

Column: Durashell RP ; 4.6 × 150 mm

Flow rate: 1.5 mL /min, 50% ACN/50% 0.05M

Ammonia (pH=9.0)

Sample: Doxepin, nortriptyline, amitriptyline,

trimipramine ,

Temperature: 35



Solutions for Low pH and High pH Applications

Venusil ASB-C18 (pH: 0.8-7.5);

Venusil ASB-C8 (pH: 1.0-7.5);

Durashell RP (pH: 1.5-12.0)

Agela Technologies offers a family of stationary phases for applications to be run at extremely low or extremely high pH. These columns

extend the capability of regular reverse phase HPLC columns with a typical pH range of 2-8, and provide more options for your applications

and method development needs.

1 2 3 4 5 6 7 8 90

1 2 3 4 5 6 7 8 90

0

10

20

30

40

50

0

20

40

60

80

100

120

140

-0.10

0.00

2.00 4.00 6.00 8.00 10.00 12.00 14.00 16.00

0.10

0.20

0.30

0.40

0.50

0.60

0.70

HP

LC

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mns

040


Solutions for LC-MSAgela Technologies provides columns that can meet the needs of LC-MS applications. The most popular LC-MS columns from Agela include:

●Unisol C18 (versatile)

●Venusil ASB-C18 and ASB-C8 (extremely low bleed and long lifetime at low pH)

●Unisol Amide (for extremely polar compounds)

Benefits:

●Low bleed and symmetric peak shape→ Sensitivity

●High retention for polar compounds → Sensitivity, versatility, and low ion suppression for bioanalytical analysis.

●Compatible with 100% water to 100% organic solvents → Simplified method development effort

●Stability Long column life means a reduced cost for customers

Pseudoephedrine in Plasma

Lignocaine

Pseudoephedrine

Pseudoephedrine

Mass system: API Qtrap 3200, Applied Biosystem

Mass condition: Positive Ion Mode, MRM

HPLC Column: Venusil ASB C18 Column 2.1mm×50mm

Part No.: VS950502-0

Sample: Sample prepared by SPE (Cleanert PCX

60mg/3mL; Part No.: CX0603)

Mobile Phase: A: 1% Formic Acid in Water B: Methanol

Gradient: 20%B to 95%B in 2 min, hold for 0.5 min, then

switch to A:B (20:80)

Flow Rate: 0.5 mL/min

Temperature: 25

CI

HC N N O

O

OH

2HCL

0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 2.2 2.4 2.6 2.8 3.0 3.2Time.min

0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 2.2 2.4 2.6 2.8 3.0 3.2Time.min

1.47

2.12

2.0e4

0.0

4.0e4

6.0e4

8.0e4

1.0e5

0.5e5

1.0e5

1.5e5

2.0e5

2.5e5

3.0e5

3.5e5

4.0e5

1.2e5

1.4e5

1.6e5

1.8e5

2.0e5

2.2e5

2.4e5

2.6e5

2.8e5

HP

LC

Colu

mns

041

Best Value



Analysis of 14 Kinds of Hormone

Mass system: Agilent 1100 Series LC/MSD Trap

HPLC Column: Venusil XBP C18 Column 2.1mm×150mm Part No.: VX951502-0

Sample: Estriol, Adosterone, Estrone+Estradial, Testosterone, Dehydro-iso-androsterone Methyl- Testosterone, Epitestosterone,

Medroxyprogestrone+Stanolone,Progesetrone, Androsterone, 5-pregen-3beta-20-one

Mobile Phase: A: Water. ;B: MeOH

Gradient: 40%B to 80%B in 24 min, then hold for 12 min.

Flow Rate: 0.8 mL /min

Temperature: 25

Oleic Acid in Plasma

Oleic acid

Mass system: API Qtrap 3200, Applied Biosystem

Mass condition: Negative Ion Mode, MRM

HPLC Column: Venusil ASB C18 Column 2.1mm×50mm Part No.: VS950502-0

Sample: Sample prepared by SPE (Cleanert PEP 60mg/3mL;Part No.: PE0603)

Mobile Phase: A: 13mmol/L ammonium acetate aq., B: Acetonitrile

Gradient: 5%B to 95%B in 2 min, hold for 2 min., switch to A:B (95:5) then hold for 2 min.


Temperature: 25



HP

LC

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mns

042


Solutions for Fast Analysis

Agela Technologies provides two approaches for fast HPLC analyses with shorter run times and higher column efficiencies.

●Small particle size or fused-core technology: Venusil AQ-C18 (2.5μm) , HALO (2.7μm)

●Unique retention mechanism : Optimix C18/Amide

HALO C18, 4.6×50mm, 2.7μm Mobile Phase: A: NH4AC Buffer (Adjust pH6.0)

B: ACN

Gradient: 5%B to 45%B in10 min.

Flow rate: 1.0 mL / min


Temperature: 35

T=7min

Conventional C18, 4.6 × 150 mm

T=16min

Mobile Phase: A: NH4AC Buffer (Adjust pH 6.0)

B: ACN

Gradient: 10%B to 60%B in 20 min.

Flow rate: 1.0 mL / min


Temperature: 35 ,

Separation by Optimix C18/Amide

Bring extremely polar and non-polar analytes closer to shorten the analysis time under isocratic conditions

t=2.023 min

t= 15.278 min

t=1.658min

t=57.216min

Mobile Phase A: Water, B: Methanol


Flow rate: 1mL/min

Detection: UV 254nm

t0= 1.52 min

Column: Optimix C18/amide,5μm, 4.6*150mm

Mobile Phase: A : B = 50:50

Column: C18, 5μm, 4.6*150mm

Mobile Phase: A : B= 60 : 40

HP

LC C

olum

ns

043


Solutions for Bio-molecules

Agela Technologies offers a broad line of HPLC columns for Bio-molecules, including reverse phase, normal phase, ion-exchange, HILIC and size

exclusion columns. All column packing materials are made of ultra pure silica, bonded with pure silanes to ensure the surface inertness.

General guide for the column selection:

1. Small peptide: C18, C8, C4; 100Å or 150 Å

2. Large peptide and proteins: C8, C4, 300 Å; ion-exchange; HILIC; size exclusion

3. Mono- and oligo-saccharides: ion-exchange; NH2; HILIC

4. Polysaccharides: ion-exchange; size exclusion

5. Oligo-nuclei: ion-exchange; reverse phase

6. Nuclei acids: ion-exchange; size exclusion

See the section of “ordering information by the type of stationary phase” for ordering or call our technical staff for helps in selecting the

columns you need.

Solutions for Preparative HPLC (See Purification Section for More Information)

Agela Technologies offers a full line of preparative HPLC columns to meet a variety of application needs.

Our preparative columns feature:

1. Great scalability

2. Excellent bed stability

3. High loading capacity

4. Broad solvent compatibility from Unisol C18, Unisol Amide and Venusil ASB-C18 (100% aqueous to 100% organic solvents)

5. Broad pH range (1-12) from Venusil Durashell

6. Unique selectivity from Unisol Amide and Venusil ASB-C18

Unisol-Amide: an unique phase from Agela Technologies that offers a good alternative selectivity to reverse phase columns and an excellent solu-

tion for purifying highly polar compounds.

Durashell RP: a high pH tolerance enable the use of high pH mobile phase, which results in great improvement of peak shape and loadability of

basic compounds.

Solutions for SFC

Agela Technologies offers a broad line of normal phase columns of different selectivity for SFC applications, including:

Unisol Amide

Venusil NH2

Venusil Silica

Venusil CN

Venusil Nitrophenyl

They are all made of high purity silica materials and are packed using our robust and reproducible process to ensure the high efficiency and the

extended lifetime.

See the section of “Ordering Information by the Type of Stationary Phase” for ordering or call our technical staff for helps.


HP

LC C

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ns

044



Reverse Phase Columns

Reverse Phase Columns for Small MoleculesAnalytical Columns, Particle Size: 5µm

Promosil C18

General C18,

100Å, 320

m2/g; C%=10

PM950202-0

PM950302-0

PM950502-0

PM951002-0

PM951505-0

PM950303-0

PM950503-0

PM951003-0

PM951503-0

PM950505-0

PM951005-0

PM951505-0

PM952005-0

PM952505-0

PM950105-0

CH-100

Dimension(mm)

2.1×20

2.1×30

2.1×50

2.1×100

2.1×150

3.0x30

3.0x50

3.0x100

3.0x150

4.6×50

4.6×100

4.6×150

4.6×200

4.6×250

4.6×10

ID: 2.1, 3.0, 4.6

PromosilC8

General C8,

100Å, 320

m2/g; C%=10

PM850202-0

PM850302-0

PM850502-0

PM851002-0

PM851505-0

PM850303-0

PM850503-0

PM851003-0

PM851503-0

PM850505-0

PM851005-0

PM851505-0

PM852005-0

PM852505-0

PM850105-0

CH-100

PromosilCN

100Å, 320

m2/g, C%=5

PM550202-0

PM550302-0

PM550502-0

PM551002-0

PM551505-0

PM550303-0

PM550503-0

PM551003-0

PM551503-0

PM550505-0

PM551005-0

PM551505-0

PM552005-0

PM552505-0

PM550105-0

CH-100

Venusil XBP Phenyl

100Å, 380

m2/g, C%=12

VX650202-0

VX650302-0

VX650502-0

VX651002-0

VX651502-0

VX650303-0

VX650503-0

VX651003-0

VX651503-0

VX650505-0

VX651005-0

VX651505-0

VX652005-0

VX652505-0

VX650105-0

CH-100

Venusil XBP C4

100Å, 380

m2/g, C%=7

VX450202-0

VX450302-0

VX450502-0

VX451002-0

VX451502-0

VX450303-0

VX450503-0

VX451003-0

VX451503-0

VX450505-0

VX451005-0

VX451505-0

VX452005-0

VX452505-0

VX450105-0

CH-100

Venusil XBP C1

100Å, 380

m2/g; C%= 3

VX150202-0

VX150302-0

VX150502-0

VX151002-0

VX151502-0

VX150303-0

VX150503-0

VX151003-0

VX151503-0

VX150505-0

VX151005-0

VX151505-0

VX152005-0

VX152505-0

VX150105-0

CH-100

Venusil XBP CN

100Å, 380

m2/g, C%=7

VC950202-0

VC950302-0

VC950502-0

VC951002-0

VC951502-0

VC950303-0

VC950503-0

VC951003-0

VC951503-0

VC950505-0

VC951005-0

VC951505-0

VC952005-0

VC952505-0

VC950105-0

CH-100

ultra surface inertness and reproducibility; balanced retention;

100Å, 380 m2/g, C%=19

same as XBP C18 with larger pore size;

150Å, 200 m2/g; C%=15

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Guard Cartridge

Cartridge Holder

Dimension(mm)

Column Type

2.1×30

2.1×50

2.1×100

2.1×150

3.0x30

3.0x50

3.0x100

3.0x150

4.6×50

4.6×100

4.6×150

4.6×200

4.6×250

4.6×10, 4/pk

20x10

5

5

5

5

5

5

5

5

5

5

5

5

5

5

VX950302-2

VX950502-2

VX951002-2

VX951502-2

VX950303-2

VX950503-2

VX951003-2

VX951503-2

VX950505-2

VX951005-2

VX951505-2

VX952005-2VX952505-2

VX950105-2

CH-200

VX950302-L

VX950502-L

VX951002-L

VX951502-L

VX950303-L

VX950503-L

VX951003-L

VX951503-L

VX950505-L

VX951005-L

VX951505-L

VX952005-L

VX952505-L

VX950105-L

CH-200

Venusil XBP C18 (2)

Venusil XBP C18 (L)

Particle Size(µm)

HP

LC C

olum

ns

045



2.1×30

2.1×50

2.1×100

2.1×150

3.0x30

3.0x50

3.0x100

3.0x150

4.6×50

4.6×100

4.6×150

4.6×200

4.6×250

G 4.6×10, 4/pk

2.1×20

2.1×30

2.1×50

2.1×100

2.1×150

3.0x30

3.0x50

3.0x100

3.0x150

G 3.0×10

4.6×50

4.6×100

4.6×150

G4.6×10

GCH 2.1, 3.0, 4.6

10×150

10×250

20×50

20×150

20×250

30×100

30×150

30×250

G 20×10

10×150

10×250

20×50

20×150

20×250

30×100

30×150

30×250

50×150

50×250

G 20×10

GCH 20x10

VS950302-0

VS950502-0

VS951002-0

VS951502-0

VS950303-0

VS950503-0

VS951003-0

VS951503-0

VS950505-0

VS951005-0

VS951505-0

VS952005-0

VS952505-0

VS950105-0

VS930202-0

VS930302-0

VS930502-0

VS931002-0

VS931502-0

VS930303-0

VS930503-0

VS931003-0

VS931503-0

VS930103-0

VS930505-0

VS931005-0

VS931505-0

VS930105-0

CH-100

VS951510-0

VS952510-0

VS950520-0

VS951520-0

VS952520-0

VS951030-0

VS951530-0

VS952530-0

VS950120-0

VS901510-0

VS902510-0

VS900520-0

VS901520-0

VS902520-0

VS901030-0

VS901530-0

VS902530-0

VS901550-0

VS902550-0

VS90120-0

CH-200

VS850302-0

VS850502-0

VS851002-0

VS851502-0

VS850303-0

VS850503-0

VS851003-0

VS851503-0

VS850505-0

VS851005-0

VS851505-0

VS852005-0

VS852505-0

VS850105-0

VS830202-0

VS830302-0

VS830502-0

VS831002-0

VS831502-0

VS830303-0

VS830503-0

VS831003-0

VS831503-0

VS830103-0

VS830502-0

VS831002-0

VS831505-0

VS830105-0

CH-200

VA950302-0

VA950502-0

VA951002-0

VA951502-0

VA950303-0

VA950503-0

VA951003-0

VA951503-0

VA950505-0

VA951005-0

VA951505-0

VA952005-0

VA952505-0

VA950105-0

VA930202-0

VA930302-0

VA930502-0

VA931002-0

VA931502-0

VA930303-0

VA930503-0

VA931003-0

VA931503-0

VA930103-0

VA930505-0

VA930505-0

VA931505-0

VA930105-0

CH-100

VA951510-0

VA952510-0

VA950520-0

VA951520-0

VA952520-0

VA951030-0

VA951530-0

VA952530-0

VA950120-0

CH-200

UO950302-0

UO950502-0

UO951002-0

UO951502-0

UO950303-0

UO950503-0

UO951003-0

UO951503-0

UO950505-0

UO951005-0

UO951505-0

UO952005-1

UO952505-0

UO950105-0

UO930202-0

UO930302-0

UO930502-0

UO931002-0

UO931502-0

UO930303-0

UO930503-0

UO931003-0

UO931503-0

UO930103-0

UO930505-0

UO931005-0

UO931505-0

UO930105-0

CH-100

UO951510-0

UO952510-0

UO950520-0

UO951520-0

UO952520-0

UO951030-0

UO951530-0

UO952530-0

UO950120-0

UO901510-0

UO902510-0

UO900520-0

UO901520-0

UO902520-0

UO901030-0

UO901530-0

UO902530-0

UO901550-0

UO902550-0

UO900120-0

CH-200

DS950302-0

DS950502-0

DS951002-0

DS951502-0

DS950303-0

DS950503-0

DS951003-0

DS951503-0

DS950505-0

DS951005-0

DS951505-0

DS952005-0

DS952505-0

DS950105-0

DS930202-0

DS930302-0

DS930502-0

DS931002-0

DS931502-0

DS930303-0

DS930503-0

DS931003-0

DS931503-0

DS930103-0

DS930505-0

DS931005-0

DS931505-0

DS930105-0

CH-100

DS951510-0

DS952510-0

DS950520-0

DS951520-0

DS952520-0

DS951030-0

DS951530-0

DS952530-0

DS950120-0

DS901510-0

DS902510-0

DS900520-0

DS901520-0

DS902520-0

DS901030-0

DS901530-0

DS902530-0

DS901550-0

DS902550-0

DS900120-0

CH-200

VX950302-0

VX950502-0

VX951002-0

VX951502-0

VX950303-0

VX950503-0

VX951003-0

VX951503-0

VX950505-0

VX951005-0

VX951505-0

VX952005-0

VX952505-0

VX950105-0

VX930202-0

VX930302-0

VX930502-0

VX931002-0

VX931502-0

VX930303-0

VX930503-0

VX931003-0

VX931503-0

VX930103-0

VX930505-0

VX931005-0

VX931505-0

VX930105-0

CH-100

VX951510-0

VX952510-0

VX950520-0

VX951520-0

VX952520-0

VX951030-0

VX951530-0

VX952530-0

VX950120-0

VX901510-0

VX902510-0

VX900520-0

VX901520-0

VX902520-0

VX901030-0

VX901530-0

VX902530-0

VX901550-0

VX902550-0

VX900120-0

CH-200

VX850302-0

VX850502-0

VX851002-0

VX851502-0

VX850303-0

VX850503-0

VX851003-0

VX851503-0

VX850505-0

VX851005-0

VX851505-0

VX852005-0

VX852505-0

VX850105-0

VX830202-0

VX830302-0

VX830502-0

VX831002-0

VX831502-0

VX830303-0

VX830503-0

VX831003-0

VX831503-0

VX830103-0

VX830502-0

VX831002-0

VX831505-0

VX830105-0

CH-100

VX851510-0

VX852510-0

VX850520-0

VX851520-0

VX852520-0

VX851030-0

VX851530-0

VX852530-0

VX850120-0

VX801510-0

VX802510-0

VX800520-0

VX801520-0

VX802520-0

VX801030-0

VX801530-0

VX802530-0

VX801550-0

VX802550-0

VX800120-0

CH-200

5

5

5

5

5

5

5

5

5

5

5

5

5

5

3

3

3

3

3

3

3

3

3

3

3

3

3

3

5

5

5

5

5

5

5

5

5

10

10

10

10

10

10

10

10

10

10

10

Venusil ASB C18

Venusil ASB C8

Venusil AQ C18

Unisol C18

Durashell RP

Venusil XBP C18

Venusil XBP C8

Particle Size(µm)

Dimension(mm)

G=Guard Cartridge; GCH= Guard Cartridge Holder

C8 for low pH and low bleed; 150 Å, 200m2/g, C%=7

extreme low pH tolerance (0.8-7.5), low bleed; 150 Å, 200m2/g; C%=12

for high aqueous applications; compatible with 100% water; 100Å, 380 m2/g, C%=18

universal RP; compatible with 100% water; 100Å, 410 m2/g, C%=17

high pH tolerance (1.5-12); 150Å,240 m2/g, C%=15

highest hydro-phobicity;100Å, 380m2/g, C%=22

general RP;100Å, 380 m2/g; C%= 14

HP

LC C

olum

ns

046


Particle Size: 5µm; Pore Size: 300Å; Surface Area: 45 m2/g

2.1×30

2.1×50

2.1×100

2.1×150

3.0x30

3.0x50

3.0x100

3.0x150

4.6×50

4.6×100

4.6×150

4.6×200

4.6×250

4.6×10, 4/pk

ID: 2.1, 3.0, 4.6

10×150

10×250

20×50

20×150

20×250

20×10

20×10

Type Dimension (mm) Venusil ASB C18 Venusil ASB C8 Venusil XBP C18 Venusil XBP C8

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Guard Cartridge

Cartridge Holder

Semi-Prep

Semi-Prep

Semi-Prep

Semi-Prep

Semi-Prep

Guard Cartridge

Cartridge Holder

extreme low pH

tolerance (0.8-7.5),

low bleed

VS950302-T

VS950502-T

VS951002-T

VS951502-T

VS950303-T

VS950503-T

VS951003-T

VS951503-T

VS950505-T

VS951005-T

VS951505-T

VS952005-T

VS952505-T

VS950105-T

CH-100

VS951510-T

VS952510-T

VS950520-T

VS951520-T

VS952520-T

VS950120-T

CH-200

C8 for low pH

and low bleed

VS850302-T

VS850502-T

VS851002-T

VS851502-T

VS850303-T

VS850503-T

VS851003-T

VS851503-T

VS850505-T

VS851005-T

VS851505-T

VS852005-T

VS852505-T

VS850105-T

CH-100

VS851510-T

VS852510-T

VS850520-T

VS851520-T

VS852520-T

VS850120-T

CH-200

highest

hydrophobicity

VX950302-T

VX950502-T

VX951002-T

VX951502-T

VX950303-T

VX950503-T

VX951003-T

VX951503-T

VX950505-T

VX951005-T

VX951505-T

VX952005-T

VX952505-T

VX950105-T

CH-100

VX951510-T

VX952510-T

VX950520-T

VX951520-T

VX952520-T

VX950120-T

CH-200

General RP

VX850302-T

VX850502-T

VX851002-T

VX851502-T

VX850303-T

VX850503-T

VX851003-T

VX851503-T

VX850505-T

VX851005-T

VX851505-T

VX852005-T

VX852505-T

VX850105-T

CH-100

VX851510-T

VX852510-T

VX850520-T

VX851520-T

VX852520-T

VX850120-T

CH-200

Reverse Phase Columns for Large MoleculesParticle Size: 5µm; Pore Size: 300Å; Surface Area: 45 m2/g

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Guard Cartridge

Cartridge Holder

Semi-prep

Semi-prep

Semi-prep

Semi-prep

Semi-prep

Guard Cartridge

Cartridge Holder

2.1×30

2.1×50

2.1×100

2.1×150

3.0x30

3.0x50

3.0x100

3.0x150

4.6×50

4.6×100

4.6×150

4.6×200

4.6×250

4.6×10, 4/pk

2.1, 3.0, 4.6

10×150

10×250

20×50

20×150

20×250

20×10

20×10

VX650302-T

VX650502-T

VX651002-T

VX651502-T

VX650303-T

VX650503-T

VX651003-T

VX651503-T

VX650505-T

VX651005-T

VX651505-T

VX652005-T

VX652505-T

VX650105-T

CH-100

VX651510-T

VX652510-T

VX650520-T

VX651520-T

VX652520-T

VX650120-T

CH-200

VX450302-T

VX450502-T

VX451002-T

VX451502-T

VX450303-T

VX450503-T

VX451003-T

VX451503-T

VX450505-T

VX451005-T

VX451505-T

VX452005-T

VX452505-T

VX450105-T

CH-100

VX451510-T

VX452510-T

VX450520-T

VX451520-T

VX452520-T

VX450120-T

CH-200

VX150302-T

VX150502-T

VX151002-T

VX151502-T

VX150303-T

VX150503-T

VX151003-T

VX151503-T

VX150505-T

VX151005-T

VX151505-T

VX152005-T

VX152505-T

VX150105-T

CH-100

VX151510-T

VX152510-T

VX150520-T

VX151520-T

VX152520-T

VX150120-T

CH-200

VC950302-T

VC950502-T

VC951002-T

VC951502-T

VC950303-T

VC950503-T

VC951003-T

VC951503-T

VC950505-T

VC951005-T

VC951505-T

VC952005-T

VC952505-T

VC950105-T

CH-100

VC951510-T

VC952510-T

VC950520-T

VC951520-T

VC952520-T

VC950120-T

CH-200

Column Type Dimension (mm) Venusil XBP Phenyl Venusil XBP C4 Venusil XBP C1 Venusil XBP CN

HP

LC C

olum

ns

047



Normal Phase Columns

Normal Phase Columns for Small MoleculesParticle Size: 5µm

Dimension (mm)

2.1×30

2.1×50

2.1×100

2.1×150

3.0x30

3.0x50

3.0x100

3.0x150

4.6×50

4.6×100

4.6×150

4.6×200

4.6×250

4.6×10 , 4/pk

2.1,3.0,4.6

10×150

10×250

20×50

20×150

20×250

30×100

30×150

30×250

20×10

10×150

10×250

20×50

20×150

20×250

30×100

30×150

30×250

50×150

50×250

20×10

2.1,3.0,4.6

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

10

10

10

10

10

10

10

10

10

10

10

Type

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Guard cartridge,

GCH

Semi-preparative

Semi-preparative

Preparative

Preparative

Preparative

Preparative

Preparative

Preparative

Guard Cartridge

Semi-preparative

Semi-preparative

Preparative

Preparative

Preparative

Preparative

Preparative

Preparative

Preparative

Preparative

Guard Cartridge

GCH

Particle Size (µm)


Venusil Silica (HILIC II)

Venusil XBP-NH2

(HILIC III)Venusil XBP-

DiolVenusil XBP

CN

unique selectivity;

100Å, 410 m2/g,

VH950302-0

VH950502-0

VH951002-0

VH951502-0

VH950303-0

VH950503-0

VH951003-0

VH951503-0

VH950505-0

VH951005-0

VH951505-0

VH952005-0

VH952505-0

VH950105-0

CH-100

VH951510-0

VH952510-0

VH950520-0

VH951520-0

VH952520-0

VH951030-0

VH951530-0

VH952530-0

VH950220-0

VH901510-0

VH902510-0

VH900520-0

VH901520-0

VH902520-0

VH901030-0

VH901530-0

VH902530-0

VH901550-0

VH902550-0

VH900120-0

CH-200

ultra pure ;

100Å, 380m2/g,

VSi950302-0

VSi950502-0

VSi951002-0

VSi951502-0

VSi950303-0

VSi950503-0

VSi951003-0

VSi951503-0

VSi950505-0

VSi951005-0

VSi951505-0

VSi952005-0

VSi952505-0

VSi950105-0

CH-100

VSi951510-0

VSi952510-0

VSi950520-0

VSi951520-0

VSi952520-0

VSi951030-0

VSi951530-0

VSi952530-0

VSi950220-0

VSi901510-0

VSi902510-0

VSi900520-0

VSi901520-0

VSi902520-0

VSi901030-0

VSi901530-0

VSi902530-0

VSi901550-0

VSi902550-0

VSi900120-0

CH-200

ultra pure; 100 Å,

380m2/g;

VN850302-0

VN850502-0

VN851002-0

VN851502-0

VN850303-0

VN850503-0

VN851003-0

VN851503-0

VN850505-0

VN851005-0

VN851505-0

VN852005-0

VN852505-0

VN850105-0

CH-100

VN851510-0

VN852510-0

VN850520-0

VN851520-0

VN852520-0

VN851030-0

VN851530-0

VN852530-0

VN850220-0

VN801510-0

VN802510-0

VN800520-0

VN801520-0

VN802520-0

VN801030-0

VN801530-0

VN802530-0

VN801550-0

VN802550-0

VN800120-0

CH-200

ultra pure; 100Å,

380 m2/g,

VC950302-0

VC950502-0

VC951002-0

VC951502-0

VC950303-0

VC950503-0

VC951003-0

VC951503-0

VC950505-0

VC951005-0

VC951505-0

VC952005-0

VC952505-0

VC950105-0

CH-100

VC951510-0

VC952510-0

VC950520-0

VC951520-0

VC952520-0

VC951030-0

VC951530-0

VC952530-0

VC950220-0

VC901510-0

VC902510-0

VC900520-0

VC901520-0

VC902520-0

VC901030-0

VC901530-0

VC902530-0

VC901550-0

VC902550-0

VC900120--0

CH-200

ultra pure; 100Å,

380 m2/g;

VD950302-0

VD950502-0

VD951002-0

VD951502-0

VD950303-0

VD950503-0

VD951003-0

VD951503-0

VD950505-0

VD951005-0

VD951505-0

VD952005-0

VD952505-0

VD950105-0

CH-100

GCH=Guard Cartridge Holder

HP

LC C

olum

ns

048


2.1×30

2.1×50

2.1×100

2.1×150

3.0x30

3.0x50

3.0x100

3.0x150

4.6×50

4.6×100

4.6×150

4.6×200

4.6×250

4.6×10 , 4/pk

2.1,3.0,4.6

10×150

10×250

20×50

20×150

20×250

30×100

30×150

30×250

20×20

Type Dimension (mm) Particle Size (µm) Promosil -CN Promosil-Silica Promosil -NH2

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Guard cartridge,

Guard cartridge Holder

Semi-preparative

Semi-preparative

Preparative

Preparative

Preparative

Preparative

Preparative

Preparative

Guard Cartridge

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

PM550302-0

PM550502-0

PM551002-0

PM551502-0

PM550303-0

PM550503-0

PM551003-0

PM551503-0

PM550505-0

PM551005-0

PM551505-0

PM552005-0

PM552505-0

PM550105-0

CH-100

PM050302-0

PM050502-0

PM051002-0

PM051502-0

PM050303-0

PM050503-0

PM051003-0

PM051503-0

PM050505-0

PM051005-0

PM051505-0

PM052005-0

PM052505-0

PM050105-0

CH-100

PM051510-0

PM052510-0

PM050520-0

PM051520-0

PM052520-0

PM051030-0

PM051530-0

PM052530-0

PM050220-0

PM750302-0

PM750502-0

PM751002-0

PM751505-0

PM750303-0

PM750503-0

PM751003-0

PM751503-0

PM750505-0

PM751005-0

PM751505-0

PM752005-0

PM752505-0

PM750105-0

CH-100

High Purity; Pore Size: 100 Å; Surface Aera: 320m2/g

HP

LC C

olum

ns

049



Normal Phase Columns for Large Molecules

Ion-exchange Columns

Ultra Pure Silica; Pore Size:300 Å; Surface Area:45m2/g

Dimension (mm)

2.1×30

2.1×50

2.1×100

2.1×150

3.0x30

3.0x50

3.0x100

3.0x150

4.6×50

4.6×100

4.6×150

4.6×200

4.6×250

4.6×10, 4/pk

ID: 2.1, 3.0, 4.6

10×150

10×250

20×50

20×150

20×250

30×100

30×150

30×250

20×10

20x10

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

Type

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Guard Cartridge

GCH

Semi-preparative

Semi-preparative

Preparative

Preparative

Preparative

Preparative

Preparative

Preparative

Guard Cartridge

GCH

Particle Size (µm)

Venusil XBP NH

2

Venusil XBP Diol

Venusil XBP CN


Venusil XBPSilica

VN850302-T

VN850502-T

VN851002-T

VN851502-T

VN850303-T

VN850503-T

VN851003-T

VN851503-T

VN850505-T

VN851005-T

VN851505-T

VN852005-T

VN852505-T

VN850105-T

CH-100

VN851510-T

VN852510-T

VN850520-T

VN851520-T

VN852520-T

VN851030-T

VN851530-T

VN852530-T

VN850120-T

CH-200

VD950302-T

VD950502-T

VD951002-T

VD951502-T

VD950303-T

VD950503-T

VD951003-T

VD951503-T

VD950505-T

VD951005-T

VD951505-T

VD952005-T

VD952505-T

VD950105-T

CH-100

VC950302-T

VC950502-T

VC951002-T

VC951502-T

VC950303-T

VC950503-T

VC951003-T

VC951503-T

VC950505-T

VC951005-T

VC951505-T

VC952005-T

VC952505-T

VC950105-T

CH-100

VC951510-T

VC952510-T

VC950520-T

VC951520-T

VC952520-T

VC951030-T

VC951530-T

VC952530-T

VC950120-T

CH-200

VSi950302-T

VSi950502-T

VSi951002-T

VSi951502-T

VSi950303-T

VSi950503-T

VSi951003-T

VSi951503-T

VSi950505-T

VSi951005-T

VSi951505-T

VSi952005-T

VSi952505-T

VSi950105-T

CH-100

VSi951510-T

VSi952510-T

VSi950520-T

VSi951520-T

VSi952520-T

VSi951030-T

VSi951530-T

VSi952530-T

VSi950120-T

CH-200

VH950302-T

VH950502-T

VH951002-T

VH951502-T

VH950303-T

VH950503-T

VH951003-T

VH951503-T

VH950505-T

VH951005-T

VH951505-T

VH952005-T

VH952505-T

VH950105-T

CH-100

Surface Aera: 200m2/g Pore Size: 150Å

Dimension(mm)

Particle Size(μm)

VenusilSCX

Type

Analytical

Analytical

Analytical

Guard cartridge

4.6×100

4.6×150

4.6×250

4.6×10 4/pk

5

5

5

5

VSc951005-0

VSc951505-0

VSc952505-0

VSc950105-0

Surface Aera: 50m2/g Pore Size: 300Å

Dimension(mm)

Particle Size(μm)

VenusilSCX

Type

Analytical

Analytical

Analytical

Guard cartridge

4.6×100

4.6×150

4.6×250

4.6×10 4/pk

5

5

5

5

VSc951005-T

VSc951505-T

VSc952505-T

VSc950105-T

VenusilSAX

VSa951005-0

VSa951505-0

VSa952505-0

VSa950105-0

VenusilSAX

VSa951005-T

VSa951505-T

VSa952505-T

VSa950105-T

GCH=Guard cartridge Holder

HP

LC

Colu

mns

050


Mixed Phase Columns

Type Dimension (mm) Particle Size(µm) Optimix C18/C8 Optimix C18/amide Optimix C18/phenyl Optimix C18/CN

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Guard cartridge

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Guard cartridge

2.1×30

2.1×50

2.1×100

2.1×150

4.6×50

4.6×100

4.6×150

4.6×200

4.6×250

4.6×10

2.1×30

2.1×50

2.1×100

2.1×150

4.6×50

4.6×100

4.6×150

4.6×10

OP950302-OC

OP950502-OC

OP951002-OC

OP951502-OC

OP950505-OC

OP951005-OC

OP951505-OC

OP952005-OC

OP952505-OC

OP950105-OC

OP930302-OC

OP930502-OC

OP931002-OC

OP931502-OC

OP930505-OC

OP931005-OC

OP931505-OC

OP930105-OC

OP950302-AM

OP950502-AM

OP951002-AM

OP951502-AM

OP950505-AM

OP951005-AM

OP951505-AM

OP952005-AM

OP952505-AM

OP950105-AM

OP930302-AM

OP930502-AM

OP931002-AM

OP931502-AM

OP930505-AM

OP931005-AM

OP931505-AM

OP930105-AM

OP950302-PH

OP950502-PH

OP951002-PH

OP951502-PH

OP950505-PH

OP951005-PH

OP951505-PH

OP952005-PH

OP952505-PH

OP950105-PH

OP930302-PH

OP930502-PH

OP931002-PH

OP931502-PH

OP930505-PH

OP931005-PH

OP931505-PH

OP930105-PH

OP950302-CN

OP950502-CN

OP951002-CN

OP951502-CN

OP950505-CN

OP951005-CN

OP951505-CN

OP952005-CN

OP952505-CN

OP950105-CN

OP930302-CN

OP930502-CN

OP931002-CN

OP931502-CN

OP930505-CN

OP931005-CN

OP931505-CN

OP930105-CN

5

5

5

5

5

5

5

5

5

5

3

3

3

3

3

3

3

3

Type Dimension (mm) Particle Size(µm) Optimix C18/SCX Optimix C18/SAX Optimix C18/Nitrophenyl

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Analytical

Guard cartridge

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Fast analysis

Guard cartridge

2.1×30

2.1×50

2.1×100

2.1×150

4.6×50

4.6×100

4.6×150

4.6×200

4.6×250

4.6×10

2.1×30

2.1×50

2.1×100

2.1×150

4.6×50

4.6×100

4.6×150

4.6×10

OP950302-SC

OP950502-SC

OP951002-SC

OP951502-SC

OP950505-SC

OP951005-SC

OP951505-SC

OP952005-SC

OP952505-SC

OP950105-SC

OP930302-SC

OP930502-SC

OP931002-SC

OP931502-SC

OP930505-SC

OP931005-SC

OP931505-SC

OP930105-SC

OP950302-SA

OP950502-SA

OP951002-SA

OP951502-SA

OP950505-SA

OP951005-SA

OP951505-SA

OP952005-SA

OP952505-SA

OP950105-SA

OP930302-SA

OP930502-SA

OP931002-SA

OP931502-SA

OP930505-SA

OP931005-SA

OP931505-SA

OP930105-SA

OP950302-NP

OP950502-NP

OP951002-NP

OP951502-NP

OP950505-NP

OP951005-NP

OP951505-NP

OP952005-NP

OP952505-NP

OP950105-NP

OP930302-NP

OP930502-NP

OP931002-NP

OP931502-NP

OP930505-NP

OP931005-NP

OP931505-NP

OP930105-NP

5

5

5

5

5

5

5

5

5

5

3

3

3

3

3

3

3

3

HP

LC C

olum

ns

051



Size Exclusion Columns (Silica Based)

Polymer Phase Columns

Dimension (mm)

4.6x 150

4.6x 250

4.6x 150

4.6x 250

10x150

10x250

10x150

10x250

5

5

10

10

5

5

10

10

Type

Analytical

Analytical

Analytical

Analytical

Semi-prep.

Semi-prep.

Semi-prep.

Semi-prep.

Particle Size (µm)

GF GF 300 GF amide GFamide-300

ultra pure,high porosity;

bonded 120Å,

GF151505-0

GF152505-0

GF101505-0

GF102505-0

GF151510-0

GF152510-0

GF101510-0

GF102510-0


bonded 300Å,

GF351505-0

GF352505-0

GF301505-0

GF302505-0

GF351510-0

GF352510-0

GF301510-0

GF302510-0


bonded with amide 120Å,

GF151505-A

GF152505-A

GF101505-A

GF102505-A

GF151510-A

GF152510-A

GF101510-A

GF102510-A


bonded with amide 300Å,

GF301505-A

GF302505-A

GF301505-A

GF302505-A

GF301510-A

GF302510-A

GF301510-A

GF302510-A

RPC-Analytical Prepacked Columns

Dimension(mm)

Particle(μm)Matrix

AKF-RP-M-154607

AKF-RP-M-154610

AKF-RP-M-154620

AKF-RP-H-254605

AKF-RP-H-254607

AKF-RP-H-254610

AKF-RP-H-254620

4.6 150

4.6 150

4.6 150

4.6 250

4.6 250

4.6 250

4.6 250

7

10

20

5

7

10

20

RPC-Preparative Prepacked Columns

Dimension(mm)

Particle(μm)Matrix

AKF-RP-M-052007

AKF-RP-M-102007

AKF-RP-M-251007

AKF-RP-M-252007

AKF-RP-M-151010

AKF-RP-M-152010

AKF-RP-H-151010

AKF-RP-H-152010

AKF-RP-M-251010

AKF-RP-M-252010

AKF-RP-H-251010

AKF-RP-H-252010

AKF-RP-H-152020

AKF-RP-H-252020

AKF-RP-H-105020

AKF-RP-H-255020

AKF-RP-H-2510020

20 50

20 100

10 250

20 250

10 150

20 150

10 150

20 150

10 250

20 250

10 250

20 250

20 150

20 250

50 100

50 250

100 250

5

5

7

7

10

10

10

10

10

10

10

10

20

20

20

20

20

HP

LC

Colu

mns

052


Ion Exchange Chromatography Columns (strong cation)

Dimension(mm)

Particle(μm)Matrix

AKF-CISLM-050207

AKF-CISLM-150207

AKF-CISLM-154607

AKF-CISLM-154610

AKF-CISLM-154620

AKF-CISLH-154607

AKF-CISLH-154610

AKF-CISLH-154620

AKF-CISLM-254610

AKF-CISLH-254610

AKF-CISLH-251010

AKF-CISLH-101010

2.0 50

2.0 150

4.6 150

4.6 150

4.6 150

4.6 150

4.6 150

4.6 150

4.6 250

4.6 250

10 250

10 100

5

5

7

10

20

7

10

20

10

10

10

10

Hydrophobic Interaction Chromatography Columns

Dimension(mm)

Particle(μm)Matrix

AKF-PHEMM-154607

AKF-PHEMM-154610

AKF-PHEMM-154620

AKF-PHEMH-254607

AKF-PHEMH-254610

AKF-PHEMH-254620

AKF-PHEMH-036410

AKF-C4-MM-154607

AKF-C4-MM-154610

AKF-C4-MM-154620

AKF-C4-MH-254607

AKF-C4-MH-254610

AKF-C4-MH-254620

AKF-C4-MH-036410

AKF-C8-MM-154607

AKF-C8-MM-154610

AKF-C8-MM-154620

AKF-C8-MH-254607

AKF-C8-MH-254610

AKF-C8-MH-254620

AKF-C8-MH-036410

AKF-ETHMM-154607

AKF-ETHMM-154610

AKF-ETHMM-154620

AKF-ETHMH-254607

AKF-ETHMH-254610

AKF-ETHMH-254620

AKF-ETHMH-036410

4.6 150

4.6 150

4.6 150

4.6 250

4.6 250

4.6 250

6.4 30

4.6 150

4.6 150

4.6 150

4.6 250

4.6 250

4.6 250

6.4 30

4.6 150

4.6 150

4.6 150

4.6 250

4.6 250

4.6 250

6.4 30

4.6 150

4.6 150

4.6 150

4.6 250

4.6 250

4.6 250

6.4 30

7

10

20

7

10

20

10

7

10

20

7

10

20

10

7

10

20

7

10

20

10

7

10

20

7

10

20

10

HP

LC

Colu

mns

053

Best Value





Ion Exchange Chromatography Columns (Weak cation)

Dimension(mm)

Particle(μm)Matrix

AKF-CLV-050207

AKF-CLV-150207

AKF-CLV-154607

AKF-CLV-154610

AKF-CLV-154620

AKF-CHV-154607

AKF-CHV-154610

AKF-CHV-154620

AKF-CLV-254610

AKF-CHV-254610

AKF-CHV-251010

AKF-CHV-101010

2.0 50

2.0 150

4.6 150

4.6 150

4.6 150

4.6 150

4.6 150

4.6 150

4.6 250

4.6 250

10 250

10 100

5

5

7

10

20

7

10

20

10

10

10

10

Ion Exchange Chromatography Columns (Strong Anion)

Dimension(mm)

Particle(μm)Matrix

AKF-QLV-050207

AKF-QLV-150207

AKF-QLV-154607

AKF-QLV-154610

AKF-QLV-154620

AKF-QHV-154607

AKF-QHV-154610

AKF-QHV-154620

AKF-QLV-254610

AKF-QHV-254610

AKF-QHV-251010

AKF-QHV-101010

2.0 50

2.0 150

4.6 150

4.6 150

4.6 150

4.6 150

4.6 150

4.6 150

4.6 250

4.6 250

10 250

10 100

5

5

7

10

20

7

10

20

10

10

10

10

Ion Exchange Chromatography Columns (Weak Anion)

Dimension(mm)

Particle(μm)Matrix

AKF-DEAL-050207

AKF-DEAL-150207

AKF-DEAL-154607

AKF-DEAL-154610

AKF-DEAL-154620

AKF-DEAH-154607

AKF-DEAH-154610

AKF-DEAH-154620

AKF-DEAL-254610

AKF-DEAH-254610

AKF-DEAH-251010

AKF-DEAH-101010

2.0 50

2.0 150

4.6 150

4.6 150

4.6 150

4.6 150

4.6 150

4.6 150

4.6 250

4.6 250

10 250

10 100

5

5

7

10

20

7

10

20

10

10

10

10

HP

LC C

olum

ns

054


Column Selection Parameters

Stationary Phases

• Reverse Phase [C18 (XBP, AQ, ASB), C8 (XBP, ASB),

C4 , Phenyl ]: most HPLC analytical and preparative

separations; use shorter chain if the retention is too

high on C18 columns use shorter chain (C8, C4) for

proteins and larger peptides.

• Normal Phase [Silica, Amino, SAX, SCX]: for those not

applicable on reverse phase; polysaccharides(amino),

ion-exchange chromatography, some preparative needs.

• Bi-mode [Cyano, Venusil HILIC]: can be used in both

reverse and normal phase modes, alternative selectivity

to hydrocarbon-based reverse phases, inert and better

reproducibility than silica columns.

Particle Size

• 3 μm: fast analysis, high throughput analytical

applications, micro/nano HPLC

• 5 μm: analytical and semi-preparative separation

• 10 μm: preparative separation

Pore Size

• Narrow pore (100-150 Å): MW <3000

• Large pore (300-500 Å): 3000< MW <50000

• Mega pore (1000 Å): MW >50000

Column Length

• Short (30 mm, 50 mm): high throughput analysis and

purification, pre-separation

• Regular (100 mm, 150 mm, 250 mm): more complex

sample, larger injection volume

Column Diameter

• Capillary (0.5 mm, 1.0 mm): LC-MS, micro-HPLC, very

small sample volume

• Analytical (2.1 mm, 4.6 mm, 10 mm): standard HPLC,

analytical and mini-prep

• Preparative (21 mm, 30 mm, 50 mm): preparative

HPLC


Basic Considerations in Choosing HPLC Columns

Analyte

• Molecular Weight – small molecule (<3000), narrow

pore; medium molecule(3000- 50000), large pore; large

molecule(>50000), mega pore.

• Solubility in aqueous solutions – very hydrophilic, use

AQ-C18, ASB-C18 or Venusil HILIC; very hydrophobic,

use shorter chain phases (C8, C4, Phenyl, CN); in

between, use AQ-C18, ASP-C18, XPB-C18 or Phenyl .

• Difference between the compounds to be separated –

by polarity, use AQ C18, ASB-C18, cyano, HILIC; by

shape or regio-isomer, use XBP-C18, phenyl.

Mobile Phase

• Solution solvent: 97-100% aqueous solution, AQ-C18

or ASB-C18; normal phase mode with aqueous mobile

phase, Venusil HILIC

• pH: pH < 2, C18 phases; pH > 9, XBP-C18; pH= 2-9,

most phases; (check the pH range for each column

before use!)

• Salt concentration: high salt concentration >0.1 M

(should be avoided if possible), XBP-C18, SCX, SAX.

Sample

• Analyte mass/concentration: high mass load, larger

diameter column (>10 for preparative).

• Sample volume: small volume, small diameter; large

volume, large diameter and length.

• Sample complexity: simple separation, short column;

complex separation, long column.

Instrument and Application

• Traditional HPLC analysis: 3μm, 5μm; 4.6 x 100 mm,

4.6 x 150 mm, 4.6 x 250 mm

• High throughput analysis: 3μm, 5μm; 2.1 x 30 mm,

2.1 x 50 mm, 4.6 x 50 mm.

• LC-MS application: 3μm, 5μm; 1.0 x 30 mm,

1.0 x 50 mm, 2.1 x 30 mm, 2.1 x 50 mm, 2.1 x 100 mm.

• Micro HPLC: 3μm, 5μm; 0.5 x 30 mm, 0.5 x 50 mm,

0.5 x 100 mm.

• Preparative HPLC: 5μm, 10μm; 21 x 50 mm,

21 x 150 mm, 21 x 250 mm, 30 x 150 mm,

30 x 250 mm, 50 x 250 mm.

HP

LC

Colu

mns

055

Best Value



Comparison of Particle Characteristics of Typical HPLC Stationary Phases

Stationary Phase Particle(μm) Pore(A) Surface(m2/g) C% End Capping High Purity Silica

Venusil XBP C18

Venusil AQ C18

Venusil ASB C18

Venusil XBP C18(L)

ZORBAX SB C18

ZORBAX XDB C18

Waters SunFire C18

Waters Symmetry C18

Waters XTerra MS C18

Hypersil BDS C18

Hypersil ODS

Inertsil ODS

Inertsil ODS3

Kromasil C18

Supelco Discovery C18

Luna C18(2)

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

5

100

100

150

150

80

80

100

100

125

130

120

100

100

100

180

100

380

380

200

200

180

180

340

335

—

170

170

350

450

340

200

400

double

yes

no

double

no

yes

yes

yes

yes

yes

yes

yes

yes

yes

yes

yes

yes

yes

yse

yes

yes

yes

yes

yes

—

no

no

no

yes

special

yes

yes

22

18

11

14

10

12

16

19

15.5

11

10

14

15

19

12

17.5



Cross-reference for Agela Venusil Series Columns

Agilent

SB C18

SB C8

XDB C18

XDB C8

SB-Aq

Waters

Sunfire C18

Sunfire C8

Atlantis C18 (AQ)

Atlantis HILIC

Phenomenex

Luna C18

Luna C8

Luna C18 (2)

Supelco

Discovery C18

Discovery C8

Asentics C18

Asentics C8

GL Science

Insertsil ODS 3

E: Similar selectivity and superior performance

R: A better replacement

R

E

E

XBP C18 XBP C18 (2)

R

E

E

XBP C8

R

E

XBP(L) C18

R

E

XBP(L) C8

R

ASB C18

R

ASB C8

R

R

E

AQ C18

R

HILIC

R

E

Venusil XBP C18 (2) 5 100 380 19 double yes

HP

LC

Colu

mns

056


USP Specifications Column Particle Shape

Octadecyl silane bonded to porous silica or ceramic

microparticles, 3 to 10μm in diameter.

Octadecyl silane chemically bonded to silica gel of a controlled surface

porosity that has been bonded to a solid spherical core, 30 to 50μm in diameter

Porous silica microparticles, 5 to 10 μm in diameter

Silica gel of controlled surface porosity bonded to

a solid spherical core, 30 to 50μm in diameter.

Alumina of controlled surface porosity bonded to a

solid spherical core, 30 to 50 μm in diameter.

Strong cation-exchange packing: sulfonated fluorocarbon

polymer coated on a solid spherical core, 30 to 50 μm in diameter.

Octylsilane bonded to totally porous microsilica

particles, 3 to 10 μm in diameter.

An essentially monomolecular layer of aminopropyl-silane chemically

bonded to totally porous silica gel support, 10 μm in diameter.

10 μm irregular totally porous silica gel having a chemically bonded,

strongly acidic cation-exchange coating.

Nitrile groups chemically bonded to porous silica

microparticles, 3 to 10 μm in diameter.

Phenyl groups chemically bonded to porous silica

micro-particles, 3 to 10 μm in diameter.

Strong anion-exchange packing made by chemically bonding a

quaternary amine to a solid silica spherical core, 30 to 50 μm in diameter

Trimethylsilane chemically bonded to porous silica

microparticles, 3 to 10 μm in diameter.

Silica gel, 10 μm in diameter, having a chemically bonded, strongly

basic quaternary ammonium anion-exchange coating.

Hexyl silane chemically bonded to totally porous

silica particles, 3 to 10 μm in diameter.

Dimethyl silane chemically bonded to totally porous


USP Column Selection Guide

Strong cation exchange resin consisting of sulfonated cross-linked

styrene-divinylbenzene copolymer in the hydrogen form, 7 to 11 μm in diameter.

Amino and cyano groups chemically bonded to

porous silica particles, 5 to 10 μm in diameter.


styrene-divinylbenzene copolymer in the calcium form, 9 μm in diameter.

Dihydroxypropane groups chemically bonded to

porous silica particles, 3 to 10 μm in diameter.

L01

L02

L03

L04

L05

L06

L07

L08

L09

L10

L11

L12

L13

L14

L15

L16

L17

L18

L19

L20

Spherical

Spherical

Spherical

Spherical

Irregular

Spherical

Spherical

Spherical

Spherical

Spherical

Spherical

Irregular

Spherical

Spherical

Venusil C18

Please call

Venusil silica

Please call

Please call

Please call

Venusil C8

Venusil NH2

Please call

Venusil CN

Venusil Phenyl

Please call

Please call

Please call

Please call

Please call

AKF CISLH columns

Please call

AKF CISLM columns

Please call

HP

LC C

olum

ns

057


USP Specifications Column Particle Shape

A rigid, spherical styrene-divinylbenzene copolymer,

5 to 10 μm in diameter.

A cation exchange resin made of porous polystyrene

gel with sulfonic acid groups, about 10 μm in size.

An ion exchange resin made of porous polymethacrylate or polyacrylate

gel with quaternary ammonium groups, about 10 μm in size.

A semi-rigid hydrophilic gel consisting of vinyl polymers with numerous

hydroxyl groups on the matrix surface, 32 to 63 μm in diameter.

Packing having the capacity to separate compounds with a MW range from

100 to 5000 daltons (as determined by polyethylene oxide), applied to neutral

Butyl silane chemically bonded to totally porous


Porous silica particles, 30 to 50 μm in diameter.

A multifunctional support, which consists of a high purity, 100 Å spherical

silica substrate that has been bonded with anionic (amine) functionality.

Gamma alumina, reversed phase, low carbon percentage by weight, alumina-based

polybutadiene spherical particles, 5 μm diameter w/a pore diameter of 80 Å

Ethyl silane chemically bonded to a totally porous

silica particle, 3 to 10 μm in diameter.

A strong anion-exchange resin-quaternary amine bonded on latex particles attached

to a core of 8.5 μm macroporous particles having a pore size of 2000 Å.

A chiral ligand-exchange packing- L-proline copper complex covanlently bonded

to irregularly shaped silica particles, 5 to 10 μm in diameter.

Packing having the capacity to separate proteins of 4,000 to 400,000 daltons.

It is spherical, silicabased and processed to provide pH stability.


styrene-divinylbenzene copolymer in the lead form, 9 μm in diameter.

A zirconium-stabilized spherical silica packing with a hydrophilic (diol-type)

molecular monolayer bonded phase having a pore size of 150 Å.

L-Phenylglycine-3,5-dinitrobenzoyl on 5 μm aminopropyl silica

Polymethacrylate gel for proteins 2000~40,000 MW

Methacrylate-based SEC column for water-solubles

Hydrophilic polyhydroxymethacrylate gel of totally porous spherical resin

Cellulose tris-3,5 dimethylphenylcarbamate coated

porous silica particles, 5 to 20 μm in diameter

L21

L22

L23

L24

L25

L26

L27

L28

L29

L30

L31

L32

L33

L34

L35

L36

L37

L38

L39

L40

Spherical

Spherical

Spherical

Spherical

Spherical

Irregular

Spherical

Spherical

Spherical

Spherical

Spherical

Spherical

Spherical

Spherical

AKF RP columns

AKF CISLH columns

Please call

Please call

Please call

Venusil C4

Please call

Please call

Please call

Please call

Please call

Please call

Please call

Please call

Please call

Nucleosil Chiral-3

Please call

Please call

Please call

Please call

USP Column Selection Guide



H

PLC

Colu

mns

058


Applications

1.Antibiotics

1.1 Benzylpenicillin Sodium

min0 2.5 5 7.5 10 12.5 15

mAU

0

50

100

150

200

250

300Benzylpenicillin Sodium

H O

NHH 2N H

N

S

O

H H

O

C H 3

C H 3

O

O N a

H

Column: Unisol C18 4.6×150mm 5μm


Mobile phase:Phosphate buffer

0.05mol/L ,pH5.0 : ACN

97.5:2.5


Flow rate: 1 mL/min

Injection: 5 μL



Mobile phase: 0.1mol/L potassium dihydrogen

phosphate buffer

pH 2.5 :ACN 70:30


Flow rate: 1 mL/min

Injection: 4 μL

1.2 Amoxicillin Sodium

H O

NHH 2N H

N

S

O

H H

O

C H 3

C H 3

O

O N a

H

min0 2 4 6

mAU

-20

0

20

40

60

80 Amoxicillin Sodium

1.3 Cefradine



Mobile phase: Water/MeOH/3.86%

sodiumacetate solution/4

acetic acid solution 742:240:15:3


Flow rate: 1 mL/min

Injection: 2μL

1.4 Cefaclor

NH

N

S

OO

O HO

C H 3

H 2N H H H

min0 5 10 15

mAU

0

50

100

150

200

250

300

350Cefradine



Mobile phase: 0.05mol/L phosphate

buffer pH 3.4 :ACN 92:8


Flow rate: 1 mL/min

Injection: 5μL

,H 2ONH

N

S

OO

O HO

C l

H 2N H H H

min0 2 4 6 8 10 12

mAU

0

10

20

30

40

50

60

70Cefaclor

HP

LC

Colu

mns

059

Best Value



1.5 Cefadroxil



Mobile phase: Water/MeOH/3.86%

sodium acetate solution/4

acetic acid solution

742:240:15:3


Flow rate: 1 mL/min

Injection: 2 μL

min0 2 4 6

mAU

0

50

100

150

200

250



Mobile phase: 0.05mol/L phosphate

buffer pH 5.5 :ACN 96:4


Flow rate: 1 mL/min

Injection: 2μL

1.6 Cefalexin

1.7 Cefotaxime Sodium



Mobile phase: phosphate buffer

0.4mM KH2PO

4+8mM K

2HPO

4

/MeOH=89:11


Flow rate: 1 mL/min

Injection: 2 μL

1.8 Sulbactam Sodium + Cefoperazone Sodium

Column: Venusil XBP C18,

(4.6×250 mm 5 μm)


Mobile phase: 0.005 mol/L TABOH

(adjust pH to 5.0 with

H3PO

4):ACN 70:30

Detection: UV 220nm

Flow rate: 1 mL/min

Injection: 2 μL

NH

N

S

OO

OHO

CH3

H2N H H H

,H2O

min0 2 4 6 8 10

Norm.

0

20

40

60

80

100 Cefalexin

N

S

NH

H2N

N

O

OCH3

N

SH H

O

O ONa

O CH3

O

min0 2 4 6 8 10 12 14

Norm.

0

10

20

30

40

50

60

70

80 Cefotaxime Sodium

min0 2.5 5 7.5 10 12.5 15

mAU

0

100

200

300

400

500

Sulbactam Sodium

Cefoperazone Sodium

O

N

NaO

H

S

OO

CH3

CH3

H

O NH

N

S

OO

ONO

HN H H H

O

OH

N

N

O

O

CH3



OO

O OH

N

H2N H

NH

H HS

CH3

,H2O

HP

LC

Colu

mns

060


1.9 Acetylspiramycin

Column: Venusil XBP-C8, (4.6×150 mm

5 μm)


Mobile phase:ammonium formate 0.2mol/L

adjust to pH 7.3 with TEA :

ACN=62:38

Detection: UV 232nm

Flow rate: 1 mL/min

Temperature: 30



Mobile phase: ACN:NaClO4/ H

3PO

4pH 2.2

=30:70

Temperature: 30


Injection: 20 μL

1.10 Meleumycin

Column: Venusil XBP C18, (4.6×250 mm

5 μm)


Mobile phase: H3PO

4 (0.025 mol/L

adjust to pH 3.0 with

TEA):ACN 87:13

Detection: UV 278nm

Flow rate: 1 mL/min

Injection: 20 μL

2.2 Ofloxacin


(4.6×250 mm 5 μm)


Mobile phase NH4AC/ KClO

4

(40mM , pH 2.0):ACN=85:15

Detection: UV 294nm

Flow rate: 1 mL/min

Injection: 4 μL

2.1 Norfloxacin

2.Synthetic antimicrobial agents

H

N

N N

F

C H 3

O H

OO

m in0 2 . 5 5 7 . 5 1 0 1 2 . 5

mAU

0

50

100

150

200

N o r f lo x a c in

N

N

H 3C

N

O

F

O

C O O H

H

C H 3

m in0 2 4 6 8 1 0

mAU

0

100

200

300

400

500

600

700

800

0

0 10 20 30 40 50 60 m

20

40

60

80

100

120

140

mAU

O

OH

HO

O

O

O

O

O

O

O OH

O O

HO

N

N

0

0 5 10 15 20 25 30 35 40 45 50 55 60 65 70-1

1

2

3

4

5

6

7

8

9

10

1.2

40

9.3

15

11

.21

5

18

.61

52

0.6

48

29

.98

2

52

24

8

57

.31

5

49

.04

8

HP

LC

Colu

mns

061

Best Value



2.3 Ciprofloxacin Lactate


(4.6×150 mm 5 μm)


Mobile phase: citric acid buffer (0.05

mol/L, adjust to pH 3.5

with TFA):ACN=82:28

Detection: UV 277nm

Flow rate: 1 mL/min

Injection: 10 μL

H N

N N

O

F C O O H

min0 1 2 3 4 5 6 7

mAU

0

25

50

75

100

125

150

175

200

Ciprofloxacin Lactate

Column: Venusil XBP C18(L),

(4.6×150mm 5 μm)

Part No.: VX951505-L

Mobile phase:1%TEA pH 4.5 :ACN=87:13

Detection: UV 325nm


Injection: 10 μL

Temperature: 40

2.4 Gatifloxacin hydrochloride

2.5 Fluconazole

Column: Venusil XBP C18, (4.6×150 mm

5 μm)


Mobile phase: KH2PO

4 buffer(adjust to

pH7.0 with NaOH):MeOH 55:45

Detection: UV 260nm

Flow rate: 1 mL/min

Injection: 10 μL

Column: Venusil XBP-C8, (4.6×250 mm

5 μm)


Mobile phase: MeOH/ACN/KH2PO

4

0.075 mol/L /citric acid

1mol/L 30:30:36:4

Detection: UV 254nm

Flow rate: 1 mL/min

Injection: 2 μL

N

N

N C H 2 C

O H

C H 2 N

N

N

F

F

min0 1 2 3 4 5 6 7

mAU

0

50

100

150

200

250

300Fluconazole

3.1 Rifampicin

3.Anti-virus medicine

min0 5 10 15 20

mAU

0

50

100

150

200

250Rifampicin

O

N

N

N

H 3C

OC H 3

O H

O

H 3C O

C H 3

O

O

H 3C

H 3C

C H 3

H 3C

C H 3

O

O HN H

C H 3

C H 3

O H

C H 3

N=8763.37

As=1.39



6.9

88

06

04

02

00

0 5 10 15 20 25 30

06

04

02

00

O

O

OH

F

F

F

HP

LC

Colu

mns

062


3.2 Ganciclovir



Mobile phase:Water:MeOH 95:5

Detection: UV 252nm

Flow rate: 1 mL/min

Injection: 2 μL

4.2 Triamcinolone Acetonide



Mobile phase: MeOH:Water 21:19

Detection: UV 240nm

Flow rate: 1 mL/min

Injection: 2 μL

4.3 Hydrocortisone



Mobile phase: MeOH:Water 70:30

Detection: UV 240nm

Flow rate: 1 mL/min

Injection: 10 μL



Mobile phase:MeOH:Water 70:30

Detection: UV 281nm

Flow rate: 1 mL/min

Injection: 2 μL

4.1 Ethinylestradiol

4. Steriod Hormones

HN

N N

N

OOH

OH

H2N

O

min0 2 4 6 8 10 12 14

Norm.

0

20

40

60

80

100

120

Ganciclovir

HO

H H

C

OHCH3

H

CH

min0 1 2 3 4

Norm.

0

200

400

600

800

1000

Ethinylestradiol

O

CH3

CH3

F

O

O

CH3

OHH

HO O

CH3

HH

H

min0 2 4 6 8

Norm.

0

50

100

150

200

250

300

350

400Triamcinolone Acetonide

O

CH3 H

CH3HO

H

H H

OH

OOH

min0 1 2 3 4 5 6 7

Norm.

0

50

100

150

200

Hydrocortisone

HP

LC

Colu

mns

063

Best Value



4.4 DexamethasoneColumn: Venusil XBP C18,

(4.6×150 mm 5 μm)


Mobile phase: citric acid buffer (0.05

mol/L, adjust to pH 3.5

with TFA):ACN=82:28

Detection: UV 277nm

Flow rate: 1 mL/min

Injection: 10 μL

m in0 2 4 6 8

N orm .

0

2 5

5 0

7 5

1 0 0

1 2 5

1 5 0

1 7 5

2 0 0

O

HOCH3

OH

O

CH3

OH

F

H

CH3

HH

Dexamethasone



Mobile phase:Water :ACN 30:70

Detection: UV 240nm

Flow rate: 1 mL/min

Injection: 2 μL

4.5 Levonorgestrel

H H

C

OH

H

CH

CH3

O

min0 2 4 6 8

Norm.

0

50

100

150

200

250 Levonorgestrel

4.6 Prednisone

O

H H

OH

HCH3

O OH

CH3O

min0 2 4 6 8 10 12 14

Norm.

0

50

100

150

200

250

Prednisone



Mobile phase: Water :THF :MeOH 668:250:62

Detection: UV 254nm

Flow rate: 1 mL/min

Injection: 2 μL

4.7 Clobetasol Propionate



Mobile phase: phosphate buffer 0.05

mol/L ,pH 2.5

/ACN/MeOH 425:475:100

Detection: UV 240nm

Flow rate: 1mL/min

Injection: 2μL

O

O

CH3HO

CH3

FH

H

H

Cl

O

CH3

CH3

O

H

min0 2 4 6 8

Norm.

0

10

20

30

40

50

60

70 Clobetasol Propionate



HP

LC

Colu

mns

064


4.8 Fluocinonide

Column: Venusil XBP C18

4.6×150mm 5μm


Mobile phase: MeOH :ACN :Water=60:10:30

Detection: UV 240nm

Flow rate: 1 mL/min

Injection: 10 μL

5.3 Omeprazole

Column: Venusil XBP-C8 4.6×250mm

5μm


Mobile phase: NaH2PO4 Buffer

(0.01 mol/L, adjust to pH7.6

with H3PO4): ACN 60:40

Detection: UV 302nm

Flow rate: 1 mL/min

Injection: 4 μL

Column: Venusil XBP C18 4.6×150mm

5μm


Mobile phase:MeOH :0.77%

Ammonium acetate aq. 285:115

Detection: UV 320nm

Flow rate: 1 mL/min

Injection: 10 μL

O

CH3

F

H

H

CH3

H

OH

OH

O

O CH3

O

5.1 Ranitidine Hydrochloride

5 Medicine for Gastric Ulcer

ON

S

HN

HN

CH3

NO2

H3C

CH3 HCl

min0 1 2 3 4 5

mAU

0

50

100

150

200

250 Ranitidine Hydrochloride

min0 2 4 6 8 10

mAU

0

25

50

75

100

125

150

175Omeprazole

5.2 Cimetidine


4.6×150mm 5μm


Mobile phase: MeOH:Water:H3PO

4:TFA

200:800:0.3:0.2

Detection: UV 220nm

Flow rate: 1mL/min

Injection: 10 μLm in0 1 2 3 4 5 6 7

mAU

0

100

200

300

400

500 Cimetidine

HN

N

S

HN

N

CN

HN

CH3

mAU

Fluoxinonide400

350

300

250

200

150

100

50

00 1 2 3 4 5 6 min

O

HN

N

N

O

O

HP

LC

Colu

mns

065

Best Value





Mobile phase: water(0.3%TEA):ACN=75:25

Detection: UV 254nm

Flow rate: 1 mL/min

Temperature: 25

6.1 Quaternary Ammonium Alkaloids from Coptidis

6. Analysis of Alkaloids

AU

0.00

0.05

0.10

0.15

0.20

0.25

0.00 5.00 10.00 15.00 20.00 25.00 30.00 35.00 40.00 45.00 50.00 55.00 60.00

Column: Venusil XBP-NH2

4.6×250mm 5μm

Part No.: VN952505-0

Mobile phase:ACN:Ethanol:3% H3PO4 aq.

80:10:10

Detection: UV 220nm

Flow rate: 1 mL/min

Injection: 20 μL

6.2 Matrine

Mobile phase: ACN: SDS/H3PO

440:60

Detection: UV 210nm

Flow rate: 1mL/min

6.3 Ephedrine from Coptidis

min0 5 10 15 20 25 30

mAU

0

5

10

15

20

25

VWD1 A, Wavelength=210 nm (F:\GTQWJ\MH000045.D)

Ephedrina hydrochloridum

Venusil XBP C18 2

4.6×150 mm, 5μm


min0 5 10 15 20 25 30

mAU

-2.5

0

2.5

5

7.5

10

12.5

15

17.5

VWD1 A, Wavelength=210 nm (YXN\07091821.D)

Ephedrina hydrochloridum

Brand Z C18 (4.6×150 mm, 5μm)



VWD1 A Wavelength=220nm(NEWSHIYP004.D)

9.0

42

3.2

95

mAU

80

60

40

20

0

0 5 10 15 20 25 min

HP

LC

Colu

mns

066


6.5 Aconitine Mesaconitine Hypaconitin

Mobile phase: MeOH: CH3COONa aq.

(0.03mol/L, adjust pH to

3.5with Acetic acid) =15:85

Detection: UV 238nm

Flow rate: 1 mL/min

Mobile phase: ACN: 2% Acetate Buffer

adjust pH to 6.5 with TEA

15:85

Detection: UV 235nm

Flow rate: 1 mL/min

6.6 Morphine from Papaver somniferum

Promosil C18 4.6×250mm 5μm

Part No.: PM952505-0

min0 10 20 30

mAU

-50

0

50

100

150

200

250

VWD1 A, Wavelength=235 nm (WUTOU\ZHENGJ26.D)

mesaconitineaconitine

hpyaconitine

Venusil XBP C18 2

4.6×250 mm, 5 μm

Part No.: VX95505-0

min0 2.5 5 7.5 10 12.5

mAU

0

20

40

60

80

100

VWD1 A, Wavelength=238 nm (YXN\07091805.D)

Codeine phosphate

Morphine

Mobile phase: ACN: 1%TFA 35:65

Detection: UV 346nm

Flow rate: 1mL/min

6.4 Berberine from Phellodendron

N

O

O

+

CH3O

CH3O

Cl- H2O2,

Venusil XBP C18 2 4.6×250 mm 5μm


min0 2.5 5 7.5 10 12.5 15 17.5

mAU

0

10

20

30

40

50

60

70

VWD1 A, Wavelength=346 nm (TF\XBJ00079.D)

Berberine hydrochloride

Brand Y ODS 4.6×250 mm 5 μm

N

O

O

+

CH3O

CH3O

Cl- H2O2,

min0 2.5 5 7.5 10 12.5 15 17.5

mAU

0

20

40

60

80

100

120

VWD1 A, Wavelength=346 nm (TF\XBJ00072.D)

Berberine hydrochloride

HP

LC

Colu

mns

067

Best Value




4.6×150 mm 5 μm


Mobile phase: ACN:Water=70:30

Detection: UV 225nm

Flow rate: 1 mL/min

Injection: 5 μL

Temperature: 25

7.2 Haloxyfop-P-methyl

7.1 Analysis of 14 Kinds Herbicides

7 Agricultural Chemical

Sample: dazomet Cymoxanil Triadimefon Tebuconazole

Thiophanate-Methyl hexaconazole flutriafol

Iprodione Metalaxyl Procymidone carboxin

Prochlora Diethofencar Triadimenol

1,2,3,6-tetrahydro-N-(trichloromethylthio)phthalimide

Column: Venusil XBP C18 4.6×150mm 5μm


Mobile phase: A: Ammonium acetate buffer B:ACN

Gradient: 40%B to 45%B in 8 min, hold for 40min.

Detection: UV 225nm Flow rate: 1 mL/min

Injection: 20 μL Temperature: 25

AU

0.0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

2.0 4.0 6.0 8.0 10.0 12.0 14.0 16.0

A

0.00 5.00 10.00 15.00 20.00 25.00 30.00 35.00 40.00 45.00

0.0040

0.0035

0.0030

0.0025

0.0020

0.0015

0.0010

0.0005

0.0000

-0.0005

7.3 Fluazifop-p-butyl


4.6×150 mm 5 μm


Mobile phase:ACN:Water=70:30

Detection: UV 225nm

Flow rate: 1 mL/min

Injection: 5 μL

Temperature: 25

AU

0.0

0.2

0

0.4

0

0.6

0

0.8

0

1.0

0

1.2

2.0 4.0 6.0 8.0 10.0 12.0 14.0 16.0 18.0 20.0


4.6×150 mm 5 μm


Mobile phase:Water:MeOH =25:75

Detection: UV 254nm

Flow rate: 1 mL/min

Injection: 10 μL

Temperature: 25

7.4 1,2-Dibenzoyl-1-tert-butylhydrazine

AU

0.00

0.20

0.40

0.60

0.80

1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00



HP

LC

Colu

mns

068


8.1 Standards of Amino Acids

8.2 Amino Acid from Salmon Calcitonin

8.Analysis of Amino Acid

Column: Venusil AA 4.6×250 mm 5 μm

Part No.: AA952505-0

Mobile phase: A: 0.1mol/L CH3COONa(Adjust pH 6.5 with Acetic Acid):Acetonitrile (93:7)

B:Water: Acetonitrile (4:1)

Gradient:

Flow rate: 1mL/min

Detection: UV 254nm

Temperature: 40

Sample: PTC-AA (derivatization of amino acids with PITC)

Time(min)

%B

0

0

11

7

13.9

12

14

15

29

34

29.1

100

37

100

37.1

0

45

0

HP

LC

Colu

mns

069

Best Value



8.3 Amino Acid from Terlipressin

Mass system: API Qtrap 3200 Applied Biosystem

Mass condition: Cation Detector Mode,MRM


Sample: Prepared sample by SPE(Cleanert PCX 60mg/3ml;Part No.: CX0603)

Mobile Phase: A: 1% Formic Acid in Water B: Methanol

Gradient: 20%B to 95%B in 2 min, hold for 0.5 min, then switch to A:B(20:80)


Temperature: 25

9.1 Pseudoephedrine in Plasma

9 Applications in LC-MS



CI

HC N N OOH

O

2HCL

Pseudoephedrine

Sample

Standards of Amino Acids

Pseudoephedrine

0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 2.2 2.4 2.6 2.8 3.0 3.2Time.min

2.12

2.0e4

0.0

4.0e4

6.0e4

8.0e4

1.0e5

1.2e5

1.4e5

1.6e5

1.8e5

2.0e5

2.2e5

2.4e5

2.6e5

2.8e5

Lignocaine

0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 2.2 2.4 2.6 2.8 3.0 3.2Time.min

1.47

0.5e5

1.0e5

1.5e5

2.0e5

2.5e5

3.0e5

3.5e5

4.0e5

HP

LC

Colu

mns

070


9.2 Oleic Acid in Plasma

Mass system: API Qtrap 3200 Applied Biosystem

Mass condition: Anion Detector Mode,MRM


Sample: Prepared sample by SPE(Cleanert PEP 60mg/3ml;Part No.: PE0603)

Mobile Phase: A: 13mmol/L ammonium acetate aq. ;B: Acetonitrile

Gradient: 5%B to 95%B in 2 min, hold for 2 min., switch to A:B(95:5) then hold for 2 min.


Temperature: 25

Oleic acid

OH

O

1.44

3.43

00.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0

1000

2000

3000

0

4992

4000

Inte

nsity.

cp

s

Time.min

00.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0

2.0e5

4.0e5

6.0e5

8.0e5

1.0e6

1.2e6

1.4e6

Inte

nsity.

cp

s

Time.min

00.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0

1.0e5

2.0e5

3.0e5

4.0e5

5.0e5

Inte

nsity.

cp

s

Time.min

4.56

HP

LC

Colu

mns

071

Best Value



10.1 Glipizide

10.others



Mobile phase: phosphate Buffer

0.1 mol/L ,pH 6.0 :MeOH=55:45

Flow rate: 1mL/min

Injection: 20 μL

10.2 Terfenadine



Mobile phase:MeOH: H3PO

4/Triethylamine

(0.1mol/L) 80:20

Detection: UV 254nm

Flow rate: 1 mL/min

Injection: 10 μL

10.3 Dopamine and Its Metabolin

Sample: Dopamine Isoprenaline Soprenaline

Column: Venusil F5PH 4.6×250mm 5μm

Part No.: VF952505-0

Mobile phase: MeOH: CH3COOH /CH3COONH4 Buffer (pH 4.5) =15:85

Detection: UV 280nm


Dopamine Isoprenaline

05101409.D: UV Chromatogram, 280 nm

0

20

40

60

Intens.

mAU

0 5 10 15 20 25 30 35 40 Time [min]



N =7587

AS=1.03

N=8 249

As=1.00

HP

LC

Colu

mns

072


10.6 Saccharin Sodium in Milk Powder

Sample: Benzoic acid, 2,4-

Hexadienoic acid, Sodium

saccharine, milk powder

Column: Venusil Unisol C18

4.6×250 mm 5 μm

Part No: UO952505-0

Mobile phase:MeOH:CH3COONH

4

(0.02 M) =5:95

Detection: UV 230nm

Flow rate: 1 mL/min

Temperature: 30

Sample: Formic acid, acetic acid, maleic

acid, succinic acid, fumaric acid

Column: Unisol C18 4.6×250 mm 5 μm


Mobile phase: 0.02M NH4AC

Aq.:MeOH=95:5

Detection: UV 215nm

Flow rate: 1 mL/min

Injection: 10 μL

Temperature: 20

10.5 Catechins in Tea

Sample: Extract of tea

Column: Venusil Unisol C18

4.6×250 mm 5 μm


Mobile phase: 0.02%H3PO

4: MeOH=81:19

Detection: UV 278nm

Flow rate: 1 mL/min

Temperature: 40

10.4 Organic Acids

Determination of organic acids in Fermentation Broth

M inutes

0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 20.0 22.5 25.0 27.5 30.0

Vo

lts

0 .00

0.05

0.10

0.15

Pe

rce

nt

80

85

90

95

100

1

2

3

4

Standard

M inutes

0 5 10 15 20 25 30 35 40 45 50

Vo

lts

0 .0

0.1

0.2

0.3

0.4

0.5

Pe

rce

nt

80

85

90

95

100

1

2

HP

LC

Colu

mns

073

Best Value



Determination of organic acids in Fermentation Broth



10.8 Tartrazine in Food

Column: Unisol C18

4.6×250 mm 5 μm


Mobile phase: A: Ammonium acetate

buffer pH 4.0 ;B: MeOH

Gradient: 35%B to 50%B in 5 min.

Detection: UV 254nm

Flow rate: 1 mL/min

Injection: 10 µL

10.9 Melamine in Feed

Column: Venusil ASB C18

4.6×250 mm 5 μm


Mobile phase:10mM Citric acid+

10mM Perfluorooctane

sulfonate( pH 3.0) :ACN=85:15

Detection: UV 240nm

Flow rate: 1 mL/min

Injection: 10 µL

Temperature: 40

10.7 DHA in the Health Food

Column: Unisol C18

4.6×250 mm 5 μm


Mobile phase:MeOH:Water =80:20

Detection: UV 254nm

Flow rate: 1 mL/min

Injection: 10 µL

HP

LC

Colu

mns

074


10.10 Tetrodotoxin TTX

Column: Venusil ASB C18

4.6×250 mm 5 μm


Mobile phase: 0.02% H3PO

4:MeOH=40:60

Detection: UV 200nm

Flow rate: 0.5mL/min

Temperature: 25

10.11 Baicalin

Column: Venusil ASB C18,

(4.6×150 mm 5 μm)


Mobile phase: MeOH : 1% acetic

acid =50:50

Detection: UV 274nm

Flow rate: 1 mL/min

Injection: 5 μL

0

4

2

6

8

10

12

1 2 3 4 5 6 7 8 9 ml

OHO

HO

NH OH

OH

OH

OO

HN

H2N=

SS

S

racemate

mA

OH

OH

OH

OH

HO

HO

O

OO

O

O

0.0 2.5 5.0 7.5 10 12.5 15 17.5 20 22.5 25 27.5 30

0.00

0.05

0.10

0.15

25

50

75

100

HP

LC

Colu

mns

075

Best Value



Column Cleaning Procedures

Due to interactions between the stationary phase and

sample components, HPLC columns may occasionally

require cleaning or regeneration. The following conditions

apply to silica-based columns. Flow rates should be

1/5 - 1/2 of the typical flow rate.

To estimate the column volume, use the following

equation:

V = column volume in mL

r = column radius in cm

L = column length in cm

APPENDIX

V=pr2 / L

UNBONDED SILICA COLUMNS (SILICA) Rinse with 10 column volumes each of: Hexane, Methylene, Chloride,

Isopropanol, Methylene Chloride. Mobile phase: Flush column with 30mL

2.5% 2,2-dimethoxy- propane and 2.5% glacial acetic acid in hexane

Rinse with 10 column volumes of: 95% Water/5% Acetonitrile (for buffer

removal) followed by 95% Acetonitrile/5% Water mobile phase

Rinse with 20 column volumes of mobile phase with buffer removed run

gradient (2x):

A) 0.1% Aqueous TFA in Water

B) 0.1% TFA in Acetonitrile/Isopropanol (1:2) 25% B 100% B for 30 minutes

Equilibrate with 10 column volumes of mobile phase.

Rinse with 10 column vol umes each of : Chloroform,Isopropanol, Methylene

chloride, mobile phase. Exception: Recommended for cleaning Luna Amino

when used in reversed phase mode:

1. Wash with at least 30 column volumes of Sodium Hydroxide (pH 11.0)

2. Flush with at least 30 column volumes of Water (HPLC grade)

3. Re-equilibrate to Mobile phase conditions.

(300 X 7.8mm size columns) Rinse with 5 column volumes of:

0.1M Phosphate buffer pH 3.0. For strongly retained proteins: Run 100%

Water to 100% Acetonitrile to 100% Water over 60 minutes or wash with

5 column volumes of SDS or 6M Guanidine Thiocyanate or 10% DMSO

Rinse with 10 column volumes of:

500mM Phosphate buffer pH 7 10% Acetic acid (Aq)

5 Column Volumes of Water

10 Column Volumes of Phosphate buffer pH 7


10 Column Volumes of Methanol


For protein removal

Follow the above procedure with this exception: Substitute 10 column

volumes of Methanol with 10 column volumes of 5M Urea or 5M

Guanidine Thiocyanate

REVERSED PHASE COLUMNS

(C18, C8, C4, C2, C1, PHENYL, CN, NH2)

REVERSED PHASE PROTEIN/PEPTIDE COLUMNS

(C18, C8, C5, C4, PHENYL)

BONDED NORMAL PHASE COLUMNS

(CN, NH2, DIOL )

GFC/SEC COLUMNS FOR PROTEINS

ION-EXCHANGE COLUMNS

(SAX, SCX, NH2, DEAE)



HP

LC

Colu

mns

076


The Methods of Maintaining Good Column Lifetime and PerformanceInject only well-prepared (filtration, liquid/liquid extraction, SPE) clean samples

Minimize pressure surge; avoid mechanical and thermo shock.

Use guard columns or on-line filtration.

Flush columns frequently using an appropriate program

Remove unstable and strongly retained components of no-interest from samples

Use low pH (1-6) mobile phase if possible.

Use organic buffer when operating at medium to high pH (6-10)

Avoid elevated temperature unless it is necessary

Add 200 ppm sodium azide in aqueous mobile phase to suppress the growth of the bacteria

Wash out all buffer salts and store the columns in high organic solution for overnight or long time storage

SPEColumns

and Wellplates

Cleanert SPE Columns, Wellplates and Media

Cleanert Plus SPE Columns and 96 Wellplates-NEW

OMM Technology for SPE-NEW

SLE Technology and Sorbents

Bonded Silica SPE

Non-silica Adsorption Sorbent

Mixed and Layered Phases

Specialized Phases

Cleanert Pre-IC


Applications


078

080

082

089

091

097

099

100

102

103

106

114

GC

Colu

mns

Cleanert SPE Columns, Wellplates and Media

Agela Technologies provides a full line of SPE sorbents, columns and wellplates.

OMM SPE Products (Optimized Molecular Modification)- PEP Plus, PEP, PAX, PCX, PWAX, PWCX and PS are all based on

polystyrene/divinylbenzene while each phase has different functionality and unique selectivity. They are highly recommended for the extrac-

tion of a wide range of compounds in pharmaceutical, agricultural, food, and environmental industries. PEP and PEP Plus are good replace-

ment of Water Oasis® HLB. Average particle diameter: 35-70μm; Average pore size: 80Å; Specific surface area: 600m2/g.

SLE(Solid supported Liquid/liquid Extraction) columns and well-plates

Specially treated diatomite materials are packed in columns and well plates. The liquid/liquid extraction is run on the surface of the materials,

which is easily automated in parallel and time-saving.

Bonded silica based SPE column

C18, C18-N, C8, NH2, COOH, Silica, PSA, PRS, SCX, SAX; All of them are made of high quality and low metal silica particles. Using the

special surface modification methodology, the activity of silica surface is reduced largely, which in turn will reduce the tailing of compounds

and will ensure high recovery and good reproducibility.

Characteristics of silica particles:

Average particle diameter: 50μm

Average pore size: 60Å

Volume of pore: 0.8cm2/g

Specific surface area: 480m2/g

Surface of silica: made by special surface modification

AgelaTechnologies

BETTER SOLUTION FOR CHROMATOGRAPHY

Cle

anert

SP

E

078

Best Value





Cle

anert

SP

E

079

Non-silica adsorption phase columns-Florisil, PestiCarb, Alumina(N, A, B). They are commonly used to remove polar interference from

non-polar samples. Cleanert adsorption sorbents have high purity, high recovery and good reproducibility. They are widely used in sample

preparation for environmental and food analysis.

Mixed and layered phases-C8/SCX, PestiCarb/NH2, PestiCarb/PSA

Specialized phases-SUL-5, DNPH-Silica,HXN

PEP,HXN,

PS,PAX,PCX,

PWAX,PWCX

PEP,Plus

Polymer SPE

Sorbents

Florisil,PestiCarb,

Alumina-N,

Alumina-A,

Alumina-B

Non-silica

Adsorption

Sorbents

DNPH-Silica

SUL-5,

HXN

Special SPE

Columns

C8/SCX

PestiCarb/NH2

Mix Sorbents

SLE

SLE Plus

Solid Supported

Liquid/liquid

Extraction

Cleanert SPE

Products

ODS,C8,CN,NH2 ,

COOH,Silica,PSA,

PRS,SCX,SAX,

Diol

Sorbents

Based on

Bonded Silica

GC

Colu

mns

AgelaTechnologies


Cle

anert

SP

E

080

Cleanert Plus SPE products use the particles with narrowly controlled size distribution, a novel frit material & design, unique pre-activation

process on reverse phase, and a pre-drying on normal phase columns.

Stable flow rate, clogging free:

The usual problem of cartridge clogging caused by the sticky and slimy samples can be completely eliminated by using the PEP Plus prod-

uct line, which makes the sample preparation easier, faster, and more reliable than ever.

No activation step is needed, saving 1/3 of work load and time:

Using the new Cleanert PEP Plus can save you 1/3 of your work load eliminating the pre-activation steps. PEP Plus does not suffer from

vacuum-drying effect, and has balanced adsorption for a wide range of analytes. PEP Plus is the first choice for reverse phase SPE meth-

ods development.

More consistent performance from normal phase by pre-drying:

The new Cleanert Florisil Plus, Cleanert Alumina Plus, Cleanert Pesticarb Plus , Cleanert Silica Plus and Cleanert NH2 Plus will give an

unprecedented performance consistency owing to the use of special frits, narrow particle distribution, and pre-drying features.

Faster and reliable alternative to liquid/liquid extraction:

The new Cleanert SLE Plus Solid Supported Liquid/Liquid Extraction columns and plates use specially treated diatomite materials to run

liquid/liquid extraction on solid supports. Cleanert SLE Plus products are designed for automation and parallel process for time saving.

Description

Easier, faster and more reliable!

Benefits:

SPE-HPLC Analysis of Terramycin in Serum of Mouse using Cleanert PEP Plus

Application Examples:

Cleanert Plus SPE Columns and 96 Wellplates

a. Wash with 2mL methanol, 2mL water Preconditioned

12 mins by gravity 0 mins

b. Load 2mL sample solution(the mouse serum which Load 2mL sample solution(the mouse serum which contains 10ppm

contains 10ppm terramycin); 15 mins by gravity terramycin); 4 mins by gravity

c. Wash with 3mL water, then drying the column fully Wash with 3mL water, then drying the column fully

d. Elute terramycin with 3mL methanol Elute terramycin with 3mL methanol

e. Evaporate the elution by nitrogen at room temperature; Evaporate the elution by nitrogen at room temperature; dissolve

dissolve the residue with 2mL mobile phase the residue with 2mL mobile phase

f. Recovery: 83.2% Recovery: 81.4%

method and the result of Cleanert PEP 60mg/3mL method and the result of Cleanert PEP Plus 60 mg/3mL

Material Sorbent Vol Tubes/box Cat. Number


30mg

50mg

60mg

100mg

200mg

500mg

15mg/well

30mg/well

50mg/well

A hydrophilic and

lipophilic balanced

material, made of

polydivinyl benzene

functionalized with

a polar group

100

100

50

50

30

30

96-wellplate

96-wellplate

96-wellplate

PE0301-P

PE0501-P

PE0603-P

PE1003-P

PE2006-P

PE5006-P

PE0152-WP

PE0302-WP

PE0502-WP

1mL

1mL

3mL

3mL

6mL

6mL

2mL

2mL

2mL

Cleanert PEP Plus

New Product!



Cle

aner

t SP

E

081

The new Cleanert Florisol Plus SPE products features a special frit design which made these columns clogging-free. The SPE columns also

offer an unique “pre-drying” function to samples by removing water that is contained in the samples. These columns have unprecedented

consist performance.

Average particle diameter: 80-100μm

Pore size: 80Å

Specific surface area: 250-300m2/g

Description

Cleanert Florisil Plus



100mg

200mg

500mg

500mg

1g

2g

100mg/well

Sodium

sulfate/Florisil

100

100

50

50

30

15

96-wellplate

FS1001-P

FS2003-P

FS5003-P

FS5006-P

FS0003-P

FS2000-P

FS1002-WP

1mL

3mL

3mL

6mL

6mL

10mL

2mL

The new Cleanert Silical Plus SPE products features a special frit design which made these columns clogging-free. The SPE columns also

offer an unique “pre-drying” function to samples by removing water that is contained in the samples. These columns have unprecedented

consist performance.

Average particle diameter: 40-60μm

Pore size: 60Å

Specific surface area: 480m2/g

Description

Cleanert Silica Plus



100mg

200mg

500mg

500mg

1g

50mg/well

100mg/well

Sodium

sulfate/Silica

100

100

50

50

30

96-wellplate

96-wellplate

SI1001-P

SI2003-P

SI5003-P

SI5006-P

SI0006-P

SI0502-WP

SI1002-WP

1mL

3mL

3mL

6mL

6mL

2mL

2mL

GC

Colu

mns

AgelaTechnologies


Cle

anert

SP

E

082

Re

tain

ed

on

th

e s

orb

en

t

Re

tain

ed

on

th

e s

orb

en

t

OMM Technology (Optimized Molecular Modification)

for SPE

Agela’ s SPE products have been developed based on a thorough understanding of the science, e.g., interactive natures of chemical mol-

ecules. Our SPE products can thus better meet customer’ s needs. Our R&D results demonstrated that the adsorption/desorption property

of the polymeric SPE materials is regulated by the types of the functional groups and the degree of substitution of the surface modification.

In general, modification with electron donor groups will help to retain the electron-deficient molecules, while modification with electron-

withdrawing groups will prefer to retain the electron-rich molecules. Different SPE materials have been developed by incorporating proper

types of functional groups and degree of substitutions on the surface, and thus providing optimized and balanced performance for all types of

molecules.

Cleanert PEP (Polar Enhanced Polymer) is a polymer based SPE sorbent which consists of hydrophilically modified divinyl benzene. Clean-

ert PEP polymer sorbent is water-wettable and it does not suffer from column drying effect. It thus offers consistent performance due to

proper wetting capability which is especially important for 96-well plates. It also has high retention for polar compounds and high capacity for

a wide spectrum of analytes.

The Cleanet PEP (polar polymer) sorbent is a unique hydrophilic-lipophilic balance material. It provides excellent wettability since the

sorbent hydrophobic surface is highly modified with polar functional groups. The SPE mechanism follows the reverse phase separation prin-

ciple.

Good Water-Wettability

Effect of Drying on Recovery - PEP Versus C18 Sorbents

Re

tain

ed

on

th

e s

orb

en

t

The electron donating and withdrawing functionalities of the sorbent allows it to have enhanced retention for polar compounds.

High Retention for Polar Compounds

New Product!

Best Value





Cle

anert

SP

E

083

Re

tain

ed

on

th

e s

orb

en

t

PEP sorbents are water-wettable, maintaining high retention and high capacity for a wide spectrum of analytes, even when the SPE column

runs dry.

Consistent Performance for Acidic, Neutral and Basic Compounds

The PEP sorbent has 2-3x more surface area and shows a dramatic increase of sample capacity compared to silica-based C18. The volume

of elution solvents can be reduced, and subsequently the total operation time (including solvent evaporation) will be shortened

High Capacity

Re

tain

ed

on

th

e s

orb

en

t

The OMM technology offers consistent surface modifications and thus batch-to-batch reproducibility. Multiple batches of PEP, PCX, and PAX

have been successfully used for various compounds with consistent results.

Excellent Batch-to-Batch Reproducibility

GC

Col

umns

AgelaTechnologies


Cle

aner

t SP

E

084 Description PEP (polar polymers, alternative to Oasis HLB)

PEP is made of polydivinylbenzene on which the surface is functionalized with vinyl prolidone. The material has a balanced hydrophilic and

hydrophobic property and can be used in the entire pH range of 1-14.

PEP can be used to extract a variety of polar and non-polar compounds. Some highly hydrophilic compounds which have little retention on

C18 columns, such as chlorinated phenols, phosphate esters and drug metabolites, can be effectively retained on PEP

Particle Characteristics

Functionalized polymer sorbents; Average particle size: 35μm.

Average pore size: 80Å; Surface area: 600m2/g

PEP(Polar Polymers)


30mg

60mg

100mg

200mg

500mg

30mg/well

50mg/well

10g

100g

Cleanert

PEP

100

50

50

30

30

96-wellplate

96-wellplate

PE0301

PE0603

PE1003

PE2006

PE5006

PE0302-W

PE0502-W

PE0010

PE0100

1mL

3mL

3mL

6mL

6mL

2mL

2mL


Description

The PEP Plus columns are packed with high quality PEP sorbents. The sorbents contain the high surface area polydivinylbenzene spherical

particles that are modified with polar groups to offer a balanced hydrophilicity and hydrophobicity. The characteristics of the sorbent are:

water-wettable (don’ t suffer from undesirable drying effect), wide usable pH range (1-14), high retention for polar compounds, and high

capacity.

PEP Plus has a novel frit material & design, and it has undergone a pre-activation process step during manufacturing. This eliminates the

need for an activation step prior to use thus reducing working load by ~30%.more than 1/3 work load can be saved.


Polar and spherica l particles: Average particle size: 40μm; Average pore size: 70Å ;Special surface area: 600m2/g



30mg

60mg

100mg

200mg

500mg

30mg/well

50mg/well

10g

100g

Cleanert

PEP Plus

100

50

50

30

30

96-wellplate

96-wellplate

PE0301-P

PE0603-P

PE1003-P

PE2006-P

PE5006-P

PE0302-WP

PE0502-WP

PE0010-P

PE0100-P

1mL

3mL

3mL

6mL

6mL

2mL

2mL

PEP Plus (Pre-conditioned and Clog Resistant) New Product!

Cle

anert

SP

E

085

Description

It is designed to overcome the limitations of traditional silica based mixed-mode SPE sorbents such as C18/SAX. It is a RP/strong anion-

exchange mixed mode polystyrene/divinylbenzene sorbent, stable from pH 0-14.


Based on functioalized polystyrene/divinylbenzene;

Average particle diameter: 40μm; Average pore size: 70Å; Volume of pore: 1.2cm2/g; Specific surface area: 600m2/g.

PAX(RP/Strong Anion Exchange)



30mg

60mg

100mg

200mg

500mg

30mg/well

50mg/well

10g

100g

Cleanert

PAX

100

50

50

30

30

96-wellplate

96-wellplate

AX0301

AX0603

AX1003

AX2006

AX5006

AX0302-W

AX0502-W

AX0010

AX0100

1mL

3mL

3mL

6mL

6mL

2mL

2mL

Description

PCX is a mixed-mode, strong cation exchange sorbent. It has reverse-phase and anion-exchange dual functionality. It high surface area

and a wide usable pH range of 0-14.


Based on Functionalized polystyrene/divinylbenzene;


PCX(RP/Strong Cation Exchange)


Cleanert

PCX


30mg

60mg

100mg

200mg

500mg

30mg/well

50mg/well

10g

100g

100

50

50

30

30

96-wellplate

96-wellplate

CX0301

CX0603

CX1003

CX2006

CX5006

CX0302-W

CX0502-W

CX0010

CX0100

1mL

3mL

3mL

6mL

6mL

2mL

2mL

Best ValueGuaranteed Product Quality



GC

Colu

mns

AgelaTechnologies


Cle

anert

SP

E

086

Description

Cleanert PWCX provides the dual modes of retention, weak cation exchange and reverse phase on a stable polymer sorbent, which

improves the retention for basic analytes.


Based on partially functionalized polystyrene/divinylbenzene;



30mg

60mg

100mg

200mg

500mg

30mg/well

50mg/well

10g

100g

Cleanert

PWCX

100

50

50

30

30

96-wellplate

96-wellplate

WCX0301

WCX0603

WCX1003

WCX2006

WCX5006

WCX0302-W

WCX0502-W

WCX0010

WCX0100

1mL

3mL

3mL

6mL

6mL

2mL

2mL


PWCX(RP/Weak Cation Exchange)

Description

Cleanert PWAX provides the dual modes of retention, weak anion exchange and reverse phase on a stable polymer sorbent , which

improves the retention for acidic analytes.


Based on partially functionalized aminopolystyrene/divinylbenzene;


PWAX(RP/Weak Anion Exchange)



30mg

60mg

100mg

200mg

500mg

30mg/well

50mg/well

10g

100g

Cleanert

PWAX

100

50

50

30

30

96-wellplate

96-wellplate

1mL

3mL

3mL

6mL

6mL

2mL

2mL

WAX0301

WAX0603

WAX1003

WAX2006

WAX5006

WAX0302-W

WAX0502-W

WAX0010

WAX0100

Cle

anert

SP

E

087

Description

PEP-ED is made of the polydivinylbenzene having a surface functionalized with a electron donating group. They can be used in the entire pH

1-14. The surface has a balanced hydrophilic and hydrophobic property and can extract a variety of polar and non-polar compounds, espe-

cially having electron-deficient structures .


Adsorption sorbents; Average particle size: 35μm.Average pore size: 80Å; Surface area: 600m2/g


Cleanert

PEP ED


PEP-ED

30mg

60mg

100mg

200mg

500mg

30mg/well

50mg/well

10g

100g

100

50

50

30

30

96-wellplate

96-wellplate

PE0301-E

PE0603-E

PE1003-E

PE2006-E

PE5006-E

PE0302-WE

PE0502-WE

PE0010-E

PE0100-E

Description

PEP-ER is made of the polydivinylbenzene having a surface functionalized with a electron withdrawing group . They can be used in the

entire pH 1-14. The surface has a balanced hydrophilic and hydrophobic property and can extract a variety of polar and non-polar com-

pounds, especially having a electron-rich structure


Adsorption sorbents; Average particle size: 35μm.Average pore size: 80Å; Surface area: 600m2/g

PEP-ER



30mg

60mg

100mg

200mg

500mg

30mg/well

50mg/well

10g

100g

Cleanert

PEP ER

100

50

50

30

30

96-wellplate

96-wellplate

1mL

3mL

3mL

6mL

6mL

2mL

2mL

PE0301-R

PE0603-R

PE1003-R

PE2006-R

PE5006-R

PE0302-WR

PE0502-WR

PE0010-R

PE0100-R

1mL

3mL

3mL

6mL

6mL

2mL

2mL

Best Value





GC

Col

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AgelaTechnologies


Cle

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E

088

Description

PS is made of non-substituted polydivinylbenzene. It has larger surface areas (>600m2/g.) and thus greater capacity than reverse phase

bonded silica.PS can be used for the extraction of non-polar and polar compounds.


Based on polystyrene/divinylbenzene; Average particle diameter: 40μm;

Average pore size: 70Å; Volume of pore: 1.2cm2/g; Specific surface area: 600m2/g.


30mg

60mg

100mg

200mg

500mg

30mg/well

50mg/well

10g

100g

Cleanert

PS

100

50

50

30

30

96-wellplate

96-wellplate

PS0301

PS0603

PS1003

PS2006

PS5006

PS0302-W

PS0502-W

PS0010

PS0100

1mL

3mL

3mL

6mL

6mL

2mL

2mL


PS

Cle

anert

SP

E

089

This technique utilizes a layer of solid material, which is a type of modified diatomaceous earth, as a support for liquid/liquid extraction.

SLE (Solid Supported Liquid/liquid Extraction)

Technology and Sorbents

Mechanism

A modified form of diatomaceous earth is packed in a cartridge.

Biological fluid can be applied to the cartridge using gravity flow.

The aqueous sample is deposited as a thin film on the hydrophilic surface.

The analytes are extracted with a water immiscible organic solvent.

Best Value





GC

Colu

mns

AgelaTechnologies


Cle

anert

SP

E

090


200mg

500mg

500mg

1g

14g

300mg/well

500mg/well

Special

treated diatomite

100

50

50

30

10

96-wellplate

96-wellplate

SL2003

SL5003

SL5006

SL0006

SL1400

SL3002-W

SL5002-W

3mL

3mL

6mL

6mL

40mL

2mL

2mL


The General Method Includes:

1. Load an aqueous sample into the column by gravity or a soft vacuum

2. Apply one or a multiple organic solvents by gravity or well-controlled vacuum

3. Vacuum the organic solution which contains the analyte out from bottom of the columns or plates, collect the solution

4. Concentrate the collected solution

Description

Solid Supported Liquid/Liquid Extraction columns and plates. They use the specially treated diatomite materials to run liquid/liquid extractions

on the solid supports, which are easily automated in parallel and time saving.

Compared with SLE, the SLE Plus has a special frit design which keeps the columns clogging-free, even for very nasty samples.


200mg

500mg

500mg

1g

14g

300mg/well

500mg/well

Special

treated diatomite

100

50

50

30

10

96-wellplate

96-wellplate

SL2003-P

SL5003-P

SL5006-P

SL0006-P

SL1400-P

SL3002-WP

SL5002-WP

3mL

3mL

3mL

6mL

40mL

2mL

2mL


The general method includes:

1. Load an aqueous sample into the column by gravity or a soft vacuum

2. Apply one or a multiple organic solvents by gravity or well-controlled vacuum

3. Vacuum the organic solution which contains the analyte out from bottom of the columns or plates, collect the solution

4. Concentrate the collected solution

SLE

SLE Plus

Description

Solid supported liquid/liquid Extraction columns and plates use specially treated diatomiteous materials as a solid support for liquid/liquid

extractions. The SPE procedures can be easily automated.

Cle

anert

SP

E

091

Description

ODS C18 products columns and plates are packed with reverse phase, octadecylsilane

bonded silica sorbents. The sorbent is double endcapped and has a high bonding density

(%C>17). These columns can be used as a replacement for BondElute C18 and Super clean

ENVI C18. These products can be used for desalting biomolecules, such as proteins and

DNAs.


Based on silica; C%: 18-19%; Average particle diameter: 50μm; Average pore size: 60Å;

Volume of pore: 0.8cm2/g;Specific surface area: 480m2/g

Bonded Silica SPE


Cleanert ODS

(C18,end-capped)


100mg

200mg

500mg

500mg

1g

50mg/well

100mg/well

10g

100g

100

50

50

30

30

96-wellplate

96-wellplate

181001

182003

185003

185006

180006

180502-W

181002-W

180010

180100

1mL

3mL

3mL

6mL

6mL

2mL

2mL

Si

O

O

Si O

Si

Si (CH3)

3

(CH2)

17CH

3

Description

ODS C18-N products have silica based reverse phase sorbents bonded with octadecylsilane

without endcapping. The extra silanol residue of the sorbent provides additional polar inter-

actions associated with surface silanol groups which enhance the retention of basic com-

pounds. These columns are similar to Agilent AccuBond C18 and BondElute C18 OH.

ODS C18(End-capped)

ODS C18-N(Non-end-capped)





Cleanert ODS-N

(C18,Non-end-capped)


100mg

200mg

500mg

500mg

1g

50mg/well

100mg/well

10g

100g

100

50

50

30

30

96-wellplate

96-wellplate

1mL

3mL

3mL

6mL

6mL

2mL

2mL

181001-N

182003-N

185003-N

185006-N

180006-N

180502-N-W

181002-N-W

180010-N

180100-N

Si

O

O

Si OH

Si (CH2)

17CH

3

Best Value





GC

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AgelaTechnologies


Cle

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SP

E

092

The property of C8 products is similar to ODS C18 products. However, this sorbent is slightly

less retentive than C18, which facilitates the elution of more hydrophobic substance. C8 is

successfully used for the extraction of both water-soluble and fat-soluble vitamins from

serum, as well as the desalting of biological macromolecules.

Description





Cleanert C8


100mg

200mg

500mg

500mg

1g

50mg/well

100mg/well

10g

100g

100

50

50

30

30

96-wellplate

96-wellplate

1mL

3mL

3mL

6mL

6mL

2mL

2mL

081001

082003

085003

085006

080006

080502-W

081002-W

080010

080100

Cyano(CN) SPE have silica based sorbents bonded with cyanopropyl functional group. This

polar sorbent exhibits both polar and non-polar interactions. It can be used for extraction of

both polar and non-polar molecules in either normal phase reverse phase mode.

Description

C8 (Octyl)

CN (Cyanopropyl)





Cleanert CN


100mg

200mg

500mg

500mg

1g

50mg/well

100mg/well

10g

100g

100

50

50

30

30

96-wellplate

96-wellplate

1mL

3mL

3mL

6mL

6mL

2mL

2mL

CN1001

CN2003

CN5003

CN5006

CN0006

CN0502-W

CN1002-W

CN0010

CN0100

Si

O

O

OSi

Si

Si(CH3)

3

(CH2)

7CH

3

Si

O

O

OHSi

Si (CH2)

3CN

Cle

anert

SP

E

093


Based on silica; Average particle diameter: 50μm; Average pore size: 60Å;



Cleanert NH2


100mg

200mg

500mg

500mg

1g

50mg/well

100mg/well

10g

100g

100

50

50

30

30

96-wellplate

96-wellplate

1mL

3mL

3mL

6mL

6mL

2mL

2mL





Cleanert PSA


100mg

200mg

500mg

500mg

1g

50mg/well

100mg/well

10g

100g

100

50

50

30

30

96-wellplate

96-wellplate

1mL

3mL

3mL

6mL

6mL

2mL

2mL

NH2 SPE products have silica based sorbents bonded with aminopropyl funtional group. This

sorbent can be used in either normal phase or reverse phase mode. It retains the analytes

either by a polar adsorption (from non-polar solution) or by weak anion exchange (from

aqueous solution). pKa=9.8.

Description

PSA SPE is similar to Cleanert-NH2. PSA has two amino groups with pKa=10.1 and 10.9

respectively. This sorbent is an anion exchanger slightly stronger than NH2. It can be used

for the extraction of metal ions by chelating interactions. It is also commonly used to remove

organic acids, pigments an metal ions from organic samples such as vegetables and fruits.

Description

NH2 (Aminopropyl)

PSA (N-aminoethyl) Aminopropyl

Si

O

O

Si OH

Si (CH2)

3NH

2

NH1001

NH2003

NH5003

NH5006

NH0006

NH0502-W

NH1002-W

NH0010

NH0100

PA1001

PA2003

PA5003

PA5006

PA0006

PA0502-W

PA1002-W

PA0010

PA0100

Si

Si

O

O

OH

Si (CH2)

3NH

2(CH

2)

2NH

2

Best Value




GC

Colu

mns

AgelaTechnologies


Cle

anert

SP

E

094

SAX SPE products have silica based sorbents bonded with a quaternary amine. This strong

anion exchanger is used to extract compounds capable of carrying a negative charge from

both aqueous and non-aqueous solutions. They are ideally suit to extraction of weak acids

and desalting of biological macromolecules, like BondElute-SAX.

Description

SAX (Strong Anion Exchanger)



Volume of pore: 0.8cm2/g;Specific surface area: 480m2/g The ion exchange degree: 0.5meq/g.



100mg

200mg

500mg

500mg

1g

50mg/well

100mg/well

10g

100g

100

50

50

30

30

96-wellplate

96-wellplate

1mL

3mL

3mL

6mL

6mL

2mL

2mL

COOH SPE products consist of a propane carboxylic acid on the inner silica surface. The

pKa of the carboxylic acid group is approximately 3.8. It is a useful orbent for quaternary

ammonium salt and other strong cations.

Description

COOH (Weak Cation Exchanger)






100mg

200mg

500mg

500mg

1g

50mg/well

100mg/well

10g

100g

100

50

50

30

30

96-wellplate

96-wellplate

1mL

3mL

3mL

6mL

6mL

2mL

2mL

Cleanert SAX

SA1001

SA2003

SA5003

SA5006

SA0006

SA0502-W

SA1002-W

SA0010

SA0100

Si O

O

OHSi

Si (CH2)

3N+(CH

3)

3Cl-

Cleanert COOH

CH1001

CH2003

CH5003

CH5006

CH0006

CH0502-W

CH1002-W

CH0010

CH0100

Si

O

Si

O

OH

Si (CH2)

3COOH

Cle

anert

SP

E

095

PRS (Propane Sulfonic Acid)






100mg

200mg

500mg

500mg

1g

50mg/well

100mg/well

10g

100g

100

50

50

30

30

96-wellplate

96-wellplate

1mL

3mL

3mL

6mL

6mL

2mL

2mL

SCX (Strong Cation Exchanger)






100mg

200mg

500mg

500mg

1g

50mg/well

100mg/well

10g

100g

100

50

50

30

30

96-wellplate

96-wellplate

1mL

3mL

3mL

6mL

6mL

2mL

2mL

PRS SPE sorbent is a silica gel based strong cation exchanger. This sorbent, consisting of a

propane sulfonic acid, has slightly less exchange caoability than SCX. It can be applied to

the extraction of weak cations, such as pyridine, with high recovery.

Description

SCX products are strong cation exchangers based on silica gel, with benzene sulfonic acid.

The sorbent is used to extract positively charged basic compounds or remove the salt from

biological samples. It can also be mixed with C18 sorbent to extract the organic bases.

Description

Si

Si

Si

O

O

OH

(CH2)

3SO

3H

Cleanert PRS

PR1001

PR2003

PR5003

PR5006

PR0006

PR0502-W

PR1002-W

PR0010

PR0100

Si

O

Si

O Si

OH

(CH2)

2SO

3-H+

Cleanert SCX

SC1001

SC2003

SC5003

SC5006

SC0006

SC0502-W

SC1002-W

SC0010

SC0100

Best Value





GC

Colu

mns

AgelaTechnologies


Cle

anert

SP

E

096

Silica SPE product has unbonded, activated irregular silica as the sorbent. This sorbent

exhibits high polar interaction and is used for the extraction of weak-polar or non-polar

compound, such as oil. In addition, the silanol groups are ionzable at intermediate pH, so it

can be used as a weak cation exchanger.

Description






100mg

200mg

500mg

500mg

1g

50mg/well

100mg/well

10g

100g

100

50

50

30

30

96-wellplate

96-wellplate

1mL

3mL

3mL

6mL

6mL

2mL

2mL

Silica based dihydroxy SPE. It is used to extract polar analytes from non-polar solutions. It is

a neutral sorbent and extracts compounds by forming hydrogen bonding or polar-polar

interaction. As an example, it can be used to extract THC.

Description

Silica

Diol






100mg

200mg

500mg

500mg

1g

50mg/well

100mg/well

10g

100g

100

50

50

30

30

96-wellplate

96-wellplate

1mL

3mL

3mL

6mL

6mL

2mL

2mL

Cleanert Silica

SI1001

SI2003

SI5003

SI5006

SI0006

SI0502-W

SI1002-W

SI0010

SI0100

Si

Si

O

OH

OH

Cleanert Diol

DI1001

DI2003

DI5003

DI5006

DI0006

DI0502-W

DI1002-W

DI0010

DI0100

Si CH2CH

2CH

2OCH

2CH CH

2

OHOH

Cle

aner

t SP

E

097

Florisil (Magnesia Silica)


Adsorption sorbents; Average particle diameter: 80-100μm (40-60μm optional);

Average pore size: 80Å; Specific surface area: 291m2/g



100mg

200mg

500mg

500mg

1g

50mg/well

100mg/well

10g

100g

100

50

50

30

30

96-wellplate

96-wellplate

1mL

3mL

3mL

6mL

6mL

2mL

2mL


Adsorption sorbents; Average particle size: 120-400 mesh.



100mg

200mg

500mg

500mg

1g

50mg/well

100mg/well

10g

100g

100

50

50

30

30

96-wellplate

96-wellplate

1mL

3mL

3mL

6mL

6mL

2mL

2mL

PestiCarb is made of graphitized carbon by a distinct surface modification process, and has

been used for sample cleanup in pesticide residues in plant or animal tissues. This sorbent

can effectively reduce the background noise and increase the sensitivity, the functions of

which are similar to Supelco Envicarb.

Description

PestiCarb is made of graphitized carbon by a distinct surface modification process, and has

been used for sample cleanup in pesticide residues in plants or animal tissues. This sorbent

can effectively reduce the background

noise and increase the sensitivity, the functions of which are similar to Supelco Envicarb.

Description

PestiCarb (Graphitized Carbon)

Non-silica Adsorption Sorbent

Florisil

(Magnesia Silica)

Cleanert Florisil

FS1001

FS2003

FS5003

FS5006

FS0006

FS0502-W

FS1002-W

FS0010

FS0100

Graphitized Carbon

Cleanert

PestiCarb

PC1001

PC2003

PC5003

PC5006

PC0006

PC0502-W

PC1002-W

PC0010

PC0100

Best ValueGuaranteed Product Quality

Innovation to Benefit CustomersOrdering Phone No.: (302) 438 8798


GC

Col

umns

AgelaTechnologies


Cle

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t SP

E

098

Alumina N sorbents(pH=7.5) can adsorb molecules by interaction with the aluminum metal

center. The neutralized surface allows interaction with compounds whose heteroatoms are

electronegative(e.g.N,S,P) or with an electron-rich ,highly aromatic structure.

Description


Adsorption sorbents;

Average particle size: 150 mesh; Average pore size: 58Å;



100mg

200mg

500mg

500mg

1g

50mg/well

100mg/well

10g

100g

100

50

50

30

30

96-wellplate

96-wellplate

1mL

3mL

3mL

6mL

6mL

2mL

2mL

Alumina N (Aluminium Oxide;Neutral)

Aluminium Oxide

(Neutral)

Cleanert

Alumina N

AL1001-N

AL2003-N

AL5003-N

AL5006-N

AL0006-N

AL0502-N-W

AL1002-N-W

AL0010-N

AL0100-N

Alumina A sorbents(pH=4.5) can be used as strong polar absorbents or mild cation exchang-

ers. This sorbent is processed with a special deactivation procedure which ensures high

analytes recovery.

Description

Alumina A (Aluminium Oxide;Acidic)



Average particle size: 150 mesh; Average pore size: 58Å;



100mg

200mg

500mg

500mg

1g

50mg/well

100mg/well

10g

100g

100

50

50

30

30

96-wellplate

96-wellplate

1mL

3mL

3mL

6mL

6mL

2mL

2mL

Aluminium Oxide(Acidic)

Cleanert

Alumina A

AL1001-A

AL2003-A

AL5003-A

AL5006-A

AL0006-A

AL0502-A-W

AL1002-A-W

AL0010-A

AL0100-A

Cle

aner

t SP

E

099



Average particle size: 150 mesh; Average pore size: 58Å.



100mg

200mg

500mg

500mg

1g

50mg/well

100mg/well

10g

100g

100

50

50

30

30

96-wellplate

96-wellplate

1mL

3mL

3mL

6mL

6mL

2mL

2mL

PestiCarb/NH2


See Pesticarb and Cleanert NH2



Alumina B products(pH=10) can be used to remove organic acids and phenols in the sample

matrix. They have been pre-treated by special deactivation to ensure high analytes recovery.

Description

PestiCarb/NH2 SPE column is packed of 500mg PestiCarb and 500mg NH2.It has been used

wildly in analysis of pesticides residue, esp. for the JapanesePositive List System. It can be

used in pesticide residue analysis, coloring matter,fatty acid and hydroxybenzene.

Description

Alumina B (Aluminium Oxide; Basic)

Aluminium Oxide

(Basic)

PestiCarb/NH2

Cleanert PestiCarb/NH2 500mg/500mg 6ml 30 PN0006

Cleanert

Alumina B

AL1001-B

AL2003-B

AL5003-B

AL5006-B

AL0006-B

AL0502-B-W

AL1002-B-W

AL0010-B

AL0100-B

Mixed and Layered Phases



GC

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E

100

Mixed mode SPE based on silica of C8 and strong cation-exchange. It is usually used

for the extraction of basic drugs from urine or blood

Description

C8/SCX


See C8 and SCX



50

50

50

30

30

96-wellplate

96-wellplate

3mL

3mL

3mL

6mL

6mL

2mL

2mL

HXN is also made of polydivinylbenzene having surface modified with vinylpyrrol-

idone. This sorbent is specially designed to extract sulfonyl ureas from water and soil

at ppb level. It is less polar than PEP and can also be used to extract, enrich and clean

up samples from mid polar to high polar compounds.

Description

HXN(Mid Polar Polymers Specially for Sulfonyl Urea Samples)


Based on polystyrene/divinylbenzene;Average particle diameter: 40μm;

Average pore size: 70Å; Volume of pore: 1.2cm2/g; Specific surface area: 600m2/g



C8/SCX

HXN(mid polar polymers specially

for sulfonyl urea samples)

Specialized Phases

Cleanert

C8/SCX

CS0503

CS1303

CS3003

CS5006

CS0006

CS0502-W

CS1002-W

CS0010

CS0100

50mg

130mg

300mg

500mg

1g

50mg/well

100mg/well

10g

100g

30mg

60mg

100mg

200mg

500mg

30mg/well

50mg/well

10g

100g

Cleanert HXN

100

50

50

30

30

96-wellplate

96-wellplate

HX0301

HX0603

HX1003

HX2006

HX5006

HX0302-W

HX0502-W

HX0010

HX0100

1mL

3mL

3mL

6mL

6mL

2mL

2mL

Cle

anert

SP

E

101

SUL-5(Specific Columns for Sulfonamides)


Adsorption sorbents.


SUL-5 specific columns for sulfonamides is specially designed for the extraction

of five sulfonamides(SM2,SMM,SMZ,SDM.SQ) in pork.

Description

SUL-5

(specific columns for sulfonamides)


Cleanert SUL-5 2g 10ml 100 SUL-5

Best Value





GC

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AgelaTechnologies


Cle

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SP

E

102

Cleanert Pre-IC:

Sample Clean-up Cartridges for the Ion Chromatography

It is a layered cartridge that contains IC- Ag resin and IC- H resin.

It is a layered cartridge that contains IC-Ba resin, IC- Ag resin and IC- H resin.

Part No.

IC-RP

IC-Ag/H

IC-Ba

IC-Na

IC-P

IC-M

IC-Ba/Ag/H

IC-Ag

IC-H

IC-A

The type of resin

divinylbenzene

resin

styrene-based,

sulfonic acid ,

cation-exchange

resin in the

barium form.

16% cross-linked,

styrene-based,

sulfonic acid ,

cation-exchange

resin in the

sodium form.

polyvinylpyrrolidone

(PVP) polymer resin

iminodiacetate

resin in the

ammonium form

16% cross-linked,

styrene-based,

sulfonic acid,

cation-exchange

resin in the silver

form.

16% cross-linked,

styrene-based,

sulfonic acid ,

cation-exchange

resin in the

hydrogen form

16% cross-linked,

styrene-based,

anion-exchange

resin in the

bicarbonate form.

Average

particle size

40µm

40µm

40µm

40µm

40µm

40µm

40µm

40µm

Sepcification

10 pk

10 pk

10 pk

10 pk

10 pk

10 pk

10 pk

10 pk

10 pk

10 pk

Capacity

300mg/1cc

cartridge

2.0-2.2meq

/1cc cartridge

2.0-2.2meq

/1cc cartridge

350mg/1cc

cartridge

0.4meq/1cc

cartridge

2.0-2.2meq

/1cc cartridge

2.0-2.2meq

/1cc cartridge

0.7meq/1cc

cartridge

Application

To remove substances such as aromatic dyes, some

aromatic carboxylic acids, hydrocarbons, and

surfactants from sample matrices

To remove SO4

2-.For reproducible, quantitative

determinations in low-ionic-strength samples, these

cartridges should be activated with a chloride-based

activating solution. Then the added chloride need to

be removed.

To remove high levels of alkaline earths and transition

metals from sample matrices without acidifying the

sample. This ensures good recovery of acid labile

analytes such as nitrite.

To remove the phenolic fraction of humic acids,

tannic acids, lignins, anthocyanins, and azodyes

from samples.

To remove transition metals and matrix elimination

of alkali and alkaline earth metals.

To remove chloride, bromide, and iodide from sample

matrices. An IC- H cartridge should be used after the

IC- Ag cartridge to remove dissolved Ag.

To remove high levels of alkaline earths and transition

metals from sample matrices and in the neutralization

of highly alkaline samples such as sodium hydroxide

or sodium carbonate.

To remove anion contaminant and neutralize the

strongly acidic sample solution.

Description

The sample preparation is necessary not only for HPLC but also for IC.

The demands for sample preparation of IC led to a development of a new SPE product family, which we named ”Cleanert Pre-IC”, and they

can perform excellently for sample preparation of IC thanks to their sorbent of high capacity and a good flow property

Cle

anert

SP

E

103

There are many factors influenced the establishment of SPE, and the following are the four main ones which must

be considered:

The guide on this page briefly outlines the decision making process required to choose a suitable extraction mechanism.

1) The Selection of Sorbent Retention Mechanism


Best Value





GC

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AgelaTechnologies


Cle

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SP

E

104

The guide on this page briefly outlines the decision making process required to choose a suitable sorbent.

For the normal phase and reversed phase SPE cartridges, the weight of sample can not be exceed the 5% of cartridges. For the ion-exchange

mode, the capacity of the ion-exchange must be considered.

The table below is the capability and eluted parameter of SPE:

2) The Selection of SPE Columns

Sample

Organic Aqueous

Dissolved in

methanol

Dissolved in

hethane

Normal Phase

(adsorption)

Cation Reversed

Phase

Reversed

Phase

Anion Reversed

Phase

Normal phase

C18,C8,CN,

PEP,PS,HXNNH2, Diol

Silica,

Florisil SCX SAX

Ionizable Non-ionizable

Specification Quality of Loading Sample The Minimum Volume of Elution

50mg/1mL

100mg/1mL

200mg/3mL

500mg/6mL

1g/6mL

2.5mg

5mg

10mg

25mg

50mg

125μL

250μL

500μL

1.2mL

2.4mL

C18,C8,PEP,PS,HXNC18,C8,PEP,PS,HXN

The selection of column packaging and the parameter of sample loading and elution

Cle

anert

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105

3) The Selection of Ideal Elution Solvent

The elution strength of reversed solvent

The elution strength of normal solvent

Hexane

Iso-octyl-alcohol

Tetrachloromethane

Chloroform

Dichloromethane

Tetrahydrofuran

Aether

Ethyl acetate

Acetone

Acetonitrile

Isopropanol

Methanol

Water

Best Value





GC

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AgelaTechnologies


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106

SPE Columns

Cleanert PAX, 30mg/1mL

SPE Procedure

1) Condition: 1mL methanol

2) Equilibration: 1mL water

3) Sample loading: 500 μL standard sample(8% NH3•H2O)

4) Washing: 1 mL 8% NH3•H2O

5) Elution: 5% Formic acid in methanol

HPLC Conditions

Applications

Determination of Oleic acid and its Metabolites in Human Plasm

Mobile phase: ACN: 3mmol/L ammonium acetate=85 15

HPLC column: Venusil ASB C18 Column 2.1×50mm

MS Conditions

MS: Qtrap 3200

Ion Source: ESI

Scan Mode: Negative mode

Single ions monitor (SIM): Oleic acid m/z 281.2; Internal standard substances C17 m/z 269.2

Multiple reactive monitor (MRM): Metabolite of oleic acid m/z 315.2 →141.1

O

HO

XIC of+MRM(7 pairs):315.2/127.1 amu from Sample 209(126) of 0712DHSA.wiff(Turbo Spray)

XIC of+MRM(7 pairs):315.2/141.1 amu from Sample 209(126) of 0712DHSA.wiff(Turbo Spray)

Max 2830.0 cps

Max 4660.0 cps

Inte

nsity.

cp

sIn

ten

sity.

cp

s

Time.min

Time.min

7.8e5

7.0e5

6.0e5

5.0e5

4.0e5

3.0e5

2.0e5

1.0e5

0.0e51 2 3 4 5 6 7 8 9 10 11

3.12

7.8e5

4000

3000

2000

1000

01 2 3 4 5 6 7 8 9 10 11

3.12

3.14

3.123.35

Cle

anert

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107

SPE Columns

Cleanert PCX, 100mg/1mL

SPE Procedure

1) Condition: 1mL methanol


3) Sample loading: 500 μL standard sample(8% NH3•H2O)

4) Washing: 1 mL 8% NH3•H2O

5) Elution: 5% Formic acid in methanol

HPLC Conditions

Determination of Pseudoephedrine Hydrochloride and its Metabolites in Plasm

Mobile phase: A:1% Formic acid;B: Methanol

Venusil ASB C18 Column 2.1×50mm


MS Conditions

MS: Qtrap 3200

Ion Source: ESI

Scan Mode: Positive mode

Multiple reactive monitor (MRM): Pseudoephedrine Hydrochloride m/z 166.0 →148.1

Internal standard substances: Lidocaine m/z 235.3→86.1

Table of Gradient Elution Program

Time(min) Mobile phase A Mobile phase B

0.00

2.00

3.00

3.50

80

5

5

80

20

95

95

20

Best Value





Cl

HC N N O

O

OH

+2HCL

XIC of+MRM(5 pairs):235.2/86.1 amu from Sample 4(Sample004) of lhl_20080124_wmhj_grd.wiff(Turbo Spray).Smoothed

XIC of+MRM(5 pairs):166.2/148.1 amu from Sample 4(Sample004) of lhl_20080124_wmhj_grd.wiff(Turbo Spray).Smoothed,Smoothed

Max 2.9e5 cps

Max 4.1e5 cps

Inte

nsity.

cp

sIn

ten

sity.

cp

s

2.122.8e5

4.0e5

3.5e5

3.0e5

2.5e5

2.0e5

1.5e5

1.0e5

0.5e5

0.0e5

2.6e5

2.4e5

2.2e5

2.0e5

1.8e5

1.6e5

1.4e5

1.2e5

1.0e5

0.8e5

0.6e5

0.4e5

0.2e5

0.0e50.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 2.2 2.4 2.6 2.8 3.0 3.2

1.47

0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 2.2 2.4 2.6 2.8 3.0 3.2

GC

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108

Description

Sample: 50ng/mL in human serum (dilute two times by pure water)

SPE column: Cleanert C18, 200mg/3mL

The Recovery of Evodiamine and Rutecarpine in Human Serum

SPE Procedure

1) Column activation: 2mL MeOH; 2mL H2O

2) Sample loading: 2mL (diluted) sample, at flow rate of 0.5mL/min

3) Washing: 1mL × 2H2O; drying using N

2.

4) Elution: 1mL× 2 Ethyl acetate

5) Evaporation: evaporate solvent under N2 at 40

6) Reconstitution: with 1mL MeOH/water (85/15) and filtrated using 0.45μm membrane

LC-MS Conditions

Determination of Evodiamine and Rutecarpine in Human Serum by LC-MS

NH

N

N

O

Me

H

NH

N

N

O

Evodiamine Rutecarpine

Agilent 1100/Trap XCT

Column: Venusil XBP-C18, 4.6 × 150mm, 5μm

Mobile phase: methanol/ammonium formate = 85/15

Flow: 0.5mL/min; Temp: 25 ; Inj: 10μL; MS: ESI

SPE Columns

Cleanert Alumina B; Cleanert SCX

SPE Procedure

1) Extract the ground fish with ACN; evaporate ACN; dissolve the

residue in CH2Cl

2.

2) Put Alumina B column on the top of SCX; wash both with 5mL ACN

3) Load the sample (2-3mL) into Alumina B

4) Wash both columns with 5mL ACN

5) Throw away Alumina B, elute the sample from SCX column using

4mL ACN/ammonium acetate(0.1M, pH 4.5)=1:1

HPLC Conditions

Extraction and Analysis of Malachite Green in Salmon

Column: Venusil XBP-C18, 4.6 × 150mm, 5μm

Mobile phase: ACN/ammonium acetate(0.1M, pH 4.5)=80:20;

Flow rate: 2mL/min; Temperature: 35 ; Detection: UV 588nm

Analyte Concentration (ng/mL) Mean Recovery(%) R.S.D.(%)

Evodiamine

Rutecarpine

98.1

97.1

91.5

90.5

5.24

2.32

2.48

3.90

520

52

510

51

H3C

CH3 CH3

ClCH3

N N

0 0.5 1 1.5 2 2.5 3

mAU

0.25

0.2

0.15

0.1

0.05

0

-0.05

-01

Cle

anert

SP

E

109

SPE Column

Cleanert C18/SCX (500 mg/3 mL, prototype)

Result

Recovery = 80-90% for 2 μg/kg dose, RSD<20%

SPE Method

1) Activate the column with 2 mL MeOH, 2 mL 10 mmol HCl aqueous solution;

2) Load 5 mL sample at the flow rate 1 mL/min

3) Rinse the column with 5 mL water, 5 mL MeOH, dry the column

4) Elute analyte with 5 mL 4% ammonia in MeOH.

5) Evaporate the solvent and reconstitute the sample in 1 mL 1% formic acid/MeOH = 9 : 1

Determination of Clenbuterol in Urine Samples by Mixed- phase SPE and LC-MS

SPE Column

Cleanert Florisil 1000mg/6mL strong cations.

LC-MS Detection

SPE Method

1) Condition: 5ml methanol, 5ml ether.

2) Sample loading: 5mL sample

3) Wash: 5ml ether

4) Elution: methanol/ether=7/3

The Analysis of Trace Chloramphenicol

13.00 13.50 14.00 14.50 15.00 15.50 16.00 16.50 17.00 17.50 18.00 18.50

2000

4000

6000

8000

10000

12000

14000TIC: 03081801.D

13. 98

14. 56

15. 91

17. 55

TAP

FF

CAP

m-CAP

Best Value





GC

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mns

AgelaTechnologies


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110

Materials

1) SPE column: Cleanert PEP, 4-nitrophenol

2) Seven kinds of hydroxybenzene: phenol, Cresol, 4-nitrophenol, 2-Chlorophenol, 2,4-Dichlorophenol, 2,4,6-Trichlorophenol, Pentachloro

phenol

SPE Procedure

1) Column Condition: 5mL Methyl tert-butyl ether/methanol(V/V=10:90) , 5mL/min;


3) Sample loading: 1L sample, 10mL/min

4) Washing: 10mL water, then drying using N2 for 20mins

5) Elution: 2mL Methyl tert-butyl ether

HPLC Conditions

Detection of Seven Hydroxybenzenes in Water (PEP)

Mobile phase: A: 1% acetic acid, B: 1% acetic acid and methanol

HPLC column: AQ C18 (4.6 × 150mm, 5μm); UV Detector

Time Mobile Phase Flow Rate (mL/min) UV (nm)

0-15min

15-30min

1

1.8

275

295

A:B=50:50

A:B=15:85

Chart 1. The chromatogram of seven kinds of hydroxybenzene

1 phenol; 2 4-nitrophenol; 3 Cresol; 4 2-Chlorophenol ; 5 2,4-Dichlorophenol; 6 2,4,6-Trichlorophenol ; 7 Pentachlorophenol

2.5 5.0 7.5 10.0 12.5 15.0 17.5 20.0 22.5 25.0 27.5 30.0

0.004

0.002

0.000

-0.002

1

2

3

4

5 6

7

Cle

anert

SP

E

111

SPE Method

Result of Recoveries of Five Kinds of Sulfonamides in Pork

1) Condition: 5ml 95% acetonitrile

2) Load: 3mL sample which is dissolved by 95% acetonitrile

3) Wash: 5mL 95% acetonitrile

4) Elute:10mL 70% acetonitrile

The Extraction of Five Kinds of Sulfonamides in Pork

Sample

2-nitroaniline and 4-nitroaniline in 0.1% ammonia aqueous solution.

SPE Procedure

1) Activation, 3mL methanol, 3mL DI water

2) Loading, 3mL sample

3) Washing: 1mL 0.1% ammonia aqueous solution

4) Elution: 3mL methanol/1%formic acid = 95/5.

Drying with N2 and reconstitute the residue using 1mL methanol.

HPLC conditions

Venusil AQ C18 HPLC column, 4.6x250mm; Mobile phase: Methanol/1%formic acid = 10/90

Cleanert PEP Application Testing Examples

Results

0

20

40

60

80

100

SM2 SMM SMZ SDM SQ

Concentration (100μg/kg)

0

20

40

60

80

100

SM2 SMM SMZ SDM SQ

Concentration(200μg/kg)

Nitroanilines

Sample 4-nitroaniline Concentr. (ppm) Peak Area(avg) Std Peak Area(avg) PEP Recovery (%) PEP

a

b

c

a

b

c

296740.5

28374.5

2830.5

464657.5

45113.5

3489.5

270652

31517

2947

456445

46537

4234

91

111

104

98

103

121

20

2

0.2

20

2

0.2

Best Value





GC

Colu

mns

AgelaTechnologies


Cle

anert

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E

112

Sample

Aniline and N, N-dimethylaniline in DI water solution.

SPE Procedure

1) Activation, 3mL methanol, 3mL DI water

2) Loading, 3mL sample

3) Washing: 1mL water

4) Elution: 3mL methanol/water = 95/5

Drying with N2 and reconstitute the sample using 1mL methanol.

HPLC conditions

Venusil AQ-C18 column, 4.6 × 250mm, 5μm Mobile phase: methanol/water = 50/50

Results

Aniline and N, N-dimethylaniline

Sample 4-nitroaniline Concentr. (ppm) Peak Area(avg) Std Peak Area(avg) PEP Recovery (%) PEP

a

b

c

a

b

c

d

360251

36686

3563

1418380

174334

19483

2032

213975

20672

2870

1398427

159897

18386

1841

60

56

81

98

92

94

91

200

20

2

200

20

2

0.2

Cle

anert

SP

E

113

SPE Procedure

1) Condition and equilibrate: 2mL methanol, 2mL water

2) Load: 4mL sample(diluted by 0.2% acetic acid)

3) Wash: 1mL water

4) Elute: 4mL methanol

HPLC conditions

Praziquantel st

methanol: water=70 : 30, UV=263nm, HPLC column: Venusil AQ C18, 4.6 × 150mm, 5μm

Praziquantel

min0 1 2 3 4 5 6 7

mAU

0

0.5

1

1.5

2

5.7

32

SPE Procedure

1) Condition and equilibrate: 1mL dichloromethane, 1mL water

2) Load: 4mL sample (diluted by 5mL 50% ACN and water and 10mM K2HPO

4)

3) Elute: 4mL methanol

HPLC conditions

Promosil C18, 20μL, ACN: H2O=60/40, UV=235nm

Gliclazide

0 1 2 3 4 5

350

300

250

200

150

100

50

0

mAU

Best Value





GC

Colu

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AgelaTechnologies


Cle

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114


Cleanert

C18

ENVI-18

LC-C18

Bond Elut

C18

AccuBOND

C18

Sep-pak

C18

Cleanert

C18-N

Bond Elut

C18-OH

AccuBOND

C8

Cleanert

C8ENVI-8

Bond Elut

C8

Sep-pak

C8

AccuBOND

CN

Cleanert

CNLC-CN

Bond Elut

CN

Sep-pak

CN

AccuBOND

NH2

Cleanert

NH2

LC-NH2Bond Elut

NH2

Sep-pak

NH2

AccuBOND

Silica

Cleanert

SilicaLC-Silica

Bond Elut

Silica

Sep-pak

Silica

Sep-pak

Diol

Oasis

HLB

Cleanert

COOHLC-WCX

Bond Elut

CBA

Cleanert

PRS

Bond Elut

PRS

Cleanert

PEP

Bond Elut

Plexa

Oasis

MAX

Cleanert

PAX

Oasis

MCX

Cleanert

PCX

Cleanert

HXN

AccuBOND

SAX

Cleanert

SAXLC-SAX

Bond Elut

SAX

AccuBOND

SCX

Cleanert

SCXLC-SCX

Bond Elut

SCX

AccuBOND

Diol

Cleanert

DiolLC-Diol

Bond Elut

Diol

Cleanert

PSA

Bond Elut

PSA

Agela Waters Supelco Aglient Varian

AccuBOND

ENV PS-DVB

Cleanert

PS

ODS C18

(Endcapped)

ODS C18-N

(Non-endcapped)

C8

CN

Cyanopropyl

NH2

Aminopropyl

Silica

COOH

(Weak cation exchanger)

PRS

(Propane sulfonic acid)

PEP

(Polar polymers)

PAX

PCX

HXN

SAX

(Strong anion exchanger)

SCX

(Strong cation exchanger)

Diol

PSA

(N-aminoethyl Aminopropyl)

PS ENVI-Chrom P

Purification Products


Features




116

116

118

120

125

AgelaAgelaTechnologies


Purific

atio

n

116

0 2 4 6 8 10 12 min

4.6mm 0.7mL / min 2 μL

0 2 4 6 8 10 12 min

20.0mm 14.87mL / min 42.4 μL

0 2 4 6 8 10 12 min

30.0mm 29.77mL / min 170 μL

0 2 4 6 8 10 12 min

50.8mm 85.37mL / min 488 μL

Features


Agela Technologies offers a full line of preparative HPLC columns to meet a variety of application needs.

Our preparative columns feature:

1.Great scalability

2.Excellent bed stability

3.High loading capacity

4.Broad solvent compatibility,from 100% aqueous to 100% organic solvents (Unisol C18, Unisol Amide, and Venusil ASB-C18)

5.Broad pH range,1-12 (Venusil Durashell)

6.Unique selectivity (Unisol Amide and Venusil ASB-C18)

Unisol-Amide: an unique phase from Agela Technologies that offers a good alternative selectivity to reverse phase columns and an excellent

solution for purifying highly polar compounds.

Durashell RP: High pH tolerance enables the use of high pH mobile phase, which results in great improvement of peak shape and loadability

of basic compounds.

Column Unisol Amiide , 4.6 x 250 mm, 5μm

Mobile phase ACN / Water = 85/15~40/60 in 30 min

Detection UV 210nm

Scalability

Best Value




Purific

atio

n

Excellent Bed Stability

Venusil XBP-C18, 20 x 150 mm, 10 μm >1000 injection

Efficiency vs. Number of lnjections

Pla

te C

ou

nt

0 125 250 375 500 625 750 875 1000

Plate Count

9000

8000

7000

6000

5000

Number of lnjectionsUracil Phenol 4CINB Toluene

XXXXXX

X

X

X

X

Mass Load Comparison

Column: C18, 20 x 150 mm, 5μm

Sample: Diphenhydramine, 100 mg/mL in DMSO

Mobile phase: 10 mM ammonia: ACN=65: 35

Injection: 800μL

0 1 2 3 4 5 6 7 8 9 n

500

400

300

200

100

0

mAU

Brand 1

0 1 2 3 4 5 6 7 8 9 n

500

400

300

200

100

0

mAU

Brand 2

0 1 2 3 4 5 6 7 8 9 n

500

400

300

200

100

0

mAU

Brand 3

0 1 2 3 4 5 6 7 8 9 n

500

400

300

200

100

0

mAUVenusil XBP-C18

117



Pur

ifica

tion

118

Analytical Preparative

Purpose Purpose

Information about the composition Obtain pure components

Interested in most or all components Interested in one or a few components

Characteristics Characteristics

Small injection Large injection

Reverse phase preferred Normal phase may be preferred

1~5mm internal diameter 1~10cm internal diameter

2-5μm packing materials >5μm packing materials

HPLC pump <10mL/min HPLC pump ≥10mL/min

Injection usually trouble free May have difficulties for sample injection

Maximize detection sensitivity Minimize detection sensitivity

Sample solubility less important Sample solubility very important

Mobile phase volatility less important Mobile phase volatility very important; no-volatile additiveis prohibited.

Analytical/Preparative HPLC Comparison


Pore Size: 100Å

Dimension

(mm)

Particle

(μm)

Venusil

XBP-C18

Venusil

XBP-C8

Venusil

Silica

Durashell RP Venusil

ASB-C18

Venusil

ASB-C8Type

Semi-preparative

Semi-preparative

Preparative

Preparative

Preparative

Preparative

Preparative

Preparative

Guard cartridge

Semi-preparative

Semi-preparative

Preparative

Preparative

Preparative

Preparative

Preparative

Preparative

Preparative

Preparative

Guard cartridge

10 150

10 250

20 50

20 150

20 250

30 100

30 150

30 250

20 10

10 150

10 250

20 50

20 150

20 250

30 100

30 150

30 250

50 150

50 250

20 10

5

5

5

5

5

5

5

5

5

10

10

10

10

10

10

10

10

10

10

10

VX951510-0

VX952510-0

VX950520-0

VX951520-0

VX952520-0

VX951030-0

VX951530-0

VX952530-0

VX950120-0

VX901510-0

VX902510-0

VX900520-0

VX901520-0

VX902520-0

VX901030-0

VX901530-0

VX902530-0

VX901550-0

VX902550-0

VX900120-0

VX851510-0

VX852510-0

VX850520-0

VX851520-0

VX852520-0

VX851030-0

VX851530-0

VX852530-0

VX850120-0

VX801510-0

VX802510-0

VX800520-0

VX801520-0

VX802520-0

VX801030-0

VX801530-0

VX802530-0

VX801550-0

VX802550-0

VX800120-0

VSi951510-0

VSi952510-0

VSi950520-0

VSi951520-0

VSi952520-0

VSi951030-0

VSi951530-0

VSi952530-0

VSi950120-0

VSi901510-0

VSi902510-0

VSi900520-0

VSi901520-0

VSi902520-0

VSi901030-0

VSi901530-0

VSi902530-0

VSi901550-0

VSi902550-0

VSi900120-0

VS951510-0

VS952510-0

VS950520-0

VS951520-0

VS952520-0

VS951030-0

VS951530-0

VS952530-0

VS950120-0

VS901510-0

VS902510-0

VS900520-0

VS901520-0

VS902520-0

VS901030-0

VS901530-0

VS902530-0

VS901550-0

VS902550-0

VS900120-0

VS851510-0

VS852510-0

VS850520-0

VS851520-0

VS852520-0

VS851030-0

VS851530-0

VS852530-0

VS850120-0

VS801510-0

VS802510-0

VS800520-0

VS801520-0

VS802520-0

VS801030-0

VS801530-0

VS802530-0

VS801550-0

VS802550-0

VS800120-0

DS951510-0

DS952510-0

DS950520-0

DS951520-0

DS952520-0

DS951030-0

DS951530-0

DS952530-0

DS950120-0

DS901510-0

DS902510-0

DS900520-0

DS901520-0

DS902520-0

DS901030-0

DS901530-0

DS902530-0

DS901550-0

DS902550-0

DS900120-0



Pur

ifica

tion

119

Pore Size: 100Å

Unisol

C18

Unisol

AmideType

Semi-preparative

Semi-preparative

Preparative

Preparative

Preparative

Preparative

Preparative

Preparative

Guard cartridge

Semi-preparative

Semi-preparative

Preparative

Preparative

Preparative

Preparative

Preparative

Preparative

Preparative

Preparative

Guard cartridge

Dimension

(mm)

10 150

10 250

20 50

20 150

20 250

30 100

30 150

30 250

20 10

10 150

10 250

20 50

20 150

20 250

30 100

30 150

30 250

50 150

50 250

20 10

Particle

(μm)

5

5

5

5

5

5

5

5

5

10

10

10

10

10

10

10

10

10

10

10

UO951510-0

UO952510-0

UO950520-0

UO951520-0

UO952520-0

UO951030-0

UO951530-0

UO952530-0

UO950120-0

UO901510-0

UO902510-0

UO900520-0

UO901520-0

UO902520-0

UO901030-0

UO901530-0

UO902530-0

UO901550-0

UO902550-0

UO900120-0

UA951510-0

UA952510-0

UA950520-0

UA951520-0

UA952520-0

UA951030-0

UA951530-0

UA952530-0

UA950120-0

UA901510-0

UA902510-0

UA900520-0

UA901520-0

UA902520-0

UA901030-0

UA901530-0

UA902530-0

UA901550-0

UA902550-0

UA900120-0



Purific

atio

n

120

Column Vol.(mL)Flash Column Size (g)

120 2000 176

80 1800 108

40 1100 54.6

20 900 30.5

12 700 17.9

4 500 8

Efficiency (phenylacetone)


Column chromatography has been and will be continuously used as a major purification method for synthetic and natural products. Traditional

silica-based flash chromatography columns have many drawbacks: 1) high surface activity which causes instability of certain compounds, 2)

bad tailing or overly-long retention of basic compounds due to acid/base interaction or metal chelating effect, and 3) poor reproducibility.

Agela Technologies Inc developed a technology that can specially deactivate the silica media. Our columns offer significantly improved perfor-

mance and much less undesirable silica surface activities than the regular flash columns. Our columns may also provide alternative selectivity.

Flash CS Silica ColumnsUltra pure silica (specially washed with acid and DI water, narrow particle size control and water content control)

Column Specification

Surface Area: 460- 520m2/g ;Average Pore Size: 52- 68Å ;Surface pH: 6.3-7.2;Water content: 3.0-5.0% ;Average Particle Size: 40-60μm

Aniline Flash Column Tests Report

Column: Agela Cleanert CS (40 g), Brand I (40 g Si)

Mobile phase: Dichloromethane : methanol = 99 : 1

Detection: 254 nm


Flow rate: 20 mL/min

Rt: Brand I: 4.373 min; Agela: 4.090 min

Sample: Phenyl acetone , 4-aminobenzoicacid

Mobile phase: Hexane/ethylacetate (gradient)

Detection: 254 nm


Chromatography Comparison

Agela Cleanert CS Brand I

Pur

ifica

tion

121

HILIC Flash Chromatography

Following the success of Agela’s Venusil HILIC HPLC columns, we are now introducing HILIC phases for the Flash Chromatography applica-

tions. Agela’s HILIC columns are compatible with polar and aqueous solvents, such methanol, acetonitrile, and water, which makes the solvent

handling much easier compared to that for the normal phase separations. Significantly, because of the easy operation principle in using Agela’s

HILIC columns, these columns are designed to last. You will get a great return of the column investment at an already truly affordable price.

1. Solution for compounds that re not soluble in non- or low polar solvents (hexane, IPA, toluene, dichloromethane).

2. Solution for highly polar compounds which have too much retention on silica columns.

3. Solution for a mixture of compounds with a broad range of polarity (non-polar, semi-polar, and high polar) with alternative selectivity.

Flash CM Silica Columns

Chromatogram Comparison of Catechol on Different Silica

The following figures present a comparison of column performance for an Agela’s deactivated silica column vs an unmodified silica flash

column.

Test:

The silica to be tested was packed into a stainless steel column of

4.6 × 150mm and tested on a HPLC system

Conditions:

Sample: Catechol 100μg/mL in mobile phase

Mobile phase: dichloromethane/methanol = 98/2

Temperature: 30oC

Detection: 254nm

Injection: 5μL

Silica Characteristics:

Average Particle Size: 50μm

Average Pore Size: 60Å

Shape: Irregular

Surface Area: 500m2/g

Unmodifiedsilica

Generallydesactivated

silica (CM)

0 1 2 3 4 5 6 7 8 9

240220200180

160140120100

80

604020

0-20-40

4.4

07

5.1

49

3.0

90

0 1 2 3 4 5 6 7 8 9 10

400

350

300

250

200

150

100

50

0




All HILIC phases are available for TLC, HPLC and Flash columns

Applications for HILIC columns are listed below:



Purific

atio

n

122

More important, you will get all the benefits at a truly affordable price, and may even lower the cost by multiple uses of the columns!

Column: 20g HILIC column

Sample: 10 mg of uridine in 3 mL hot water

Mobile phase: water

Detection: 254 nm


Sample: 100mg VC and 70mg VB2, direct solid loading

Mobile phase: 0-2min EtOAc; 2-22min MeOH(0-100%); 22-52min MeOH

Detection: 280 nm


Sample: 100mg VC and 70mg VB2, direct solid loading

Mobile phase: 0.1% TFA : ACN = 99 : 1

Detection: 280 nm


20g silica column 20g HILIC column

Quick Work-up CartridgeThey are specially designed to replace the conventional aqueous work up /liquid-liquid extraction process as a part of organic synthesis and

purification.They are the through-columns packed with wet resin and/or diatomite.

How to use :

1 a synthetic mixture is injected directly injected onto a column.

2 chose a solvent which is not immiscible with water to rinse the desired compounds out of column.

3 concentrate the collected solution

4 add the concentrated mixture to a flash column or a HPLC column for further separation.

Phase Size Type Cat. No.

Celite

C18 (RP)

HILIC

SCX (retaining amines)

SAX (retaining acids)

PEP (RP)

Catalyst(metal) remover

1mL

3mL

6mL

1mL

3mL

6mL

1mL

3mL

6mL

1mL

3mL

6mL

1mL

3mL

6mL

1mL

3mL

6mL

1mL

3mL

6mL

I

II

II

I

II

II

I

II

II

I

II

II

I

II

II

I

II

II

I

II

II

QWC001

QWC003

QWC006

QW9001

QW9003

QW9006

QWH001

QWH003

QWH006

QWS001

QWS003

QWS006

QWA001

QWA003

QWA006

QWP001

QWP003

QWP006

QWM001

QWM003

QWM006

Purific

atio

n

123

Ordering Information(Compatible with ISCO, Buchi and Biotage flash chromatography instruments)

CS: regular silica; CM: general deactivated, low metal and less retentive; NH2: aminopropyl; ODS: C18 reverse phase; HILIC: Hydrophilic

interaction column.

Quantity

(pk)Surface Type

Silica (CS)

Silica (CM)

Silica NH2

Silica ODS

HILIC

Silica Amount

(g)

4

12

20

40

80

120

330

800

1500

2500

4

12

20

40

80

120

330

800

4

12

20

40

80

120

330

800

4

12

20

40

80

120

330

800

4

12

20

40

80

120

330

800

Cat.

Number

CS140004-0

CS140012-0

CS140020-0

CS140040-0

CS140080-0

CS140120-0

CS140330-0

CS140800-0

CS141500-0

CS142500-0

CM140004-0

CM140012-0

CM140020-0

CM140040-0

CM140080-0

CM140120-0

CM140330-0

CM140800-0

NH140004-0

NH140012-0

NH140020-0

NH140040-0

NH140080-0

NH140120-0

NH140330-0

NH140800-0

OD140004-0

OD140012-0

OD140020-0

OD140040-0

OD140080-0

OD140120-0

OD140330-0

OD140800-0

CH140004-0

CH140012-0

CH140020-0

CH140040-0

CH140080-0

CH140120-0

CH140330-0

CS140800-0

20

20

20

10

5

5

1

1

1

1

20

20

20

10

5

5

1

1

20

20

20

10

5

5

1

1

20

20

20

10

5

5

1

1

20

20

10

10

5

5

1

1

Best Value






Purific

atio

n

124

Bulk Silica

Cat. No.

CS605005-P

CS605025-P

CS605005-G

CS605025-G

CS605001-P

CS605002-P

CS605001-G

CS605002-G

CS608005-P

CS608025-P

CS608005-G

CS608025-G

CS608001-P

CS608002-P

CS608001-G

CS608002-G

Grade

P

P

G

G

P

P

G

G

P

P

G

G

P

P

G

G

Particle Size

40-60µm

40-60µm

40-60µm

40-60µm

40-60µm

40-60µm

40-60µm

40-60µm

70-90µm

70-90µm

70-90µm

70-90µm

70-90µm

70-90µm

70-90µm

70-90µm

Pack

5kg

25kg

5kg

25kg

1kg

2.5kg

1kg

2.5kg

5kg

25kg

5kg

25kg

1kg

2.5kg

1kg

2.5kg

Fritted Empty Cartridges for Filtration or Solid Sample Loading with Screw Caps

Volume (mL)

3

6

10

30

60

120

Cat. No.

SLC 0003

SLC 0006

SLC 0008

SLC 0024

SLC 0040

SLC 0120

Quantity (pk)

50

50

50

30

20

20

P: Reagent Grade; G: Tech.Grade

Piston Seals, Cartridge

10 mL

30 mL

50 mL

SLS 0008

SLS 0024

SLS 0040

Volume (mL) Cat. No. Quantity (pk)

1

1

1

Purific

atio

n

125


Flash chromatography, is a rapid form of preparative column chromatography, is especially useful in separation and purification of large scale of

complex organic intermixture, combinatorial chemistry, drug discovery and natural product research and development.

Agela FS-2100 "Chemistry-centric" design for all purpose liquid chromatography applications

Windows interface wizard method development software with touch screen makes purification a snap,we can adjust the distance between the

touch screen and the manager

Compact Profile design for limited fume hood space

Column accepts any compression module/cartridge combination

Pump Patented valveless metering pump, maintenance free, low pulse. Suitable for all organic solvent, good resistance for acid and alkali.

Automatic pressure monitoring & alarm for over/under-pressure conditions

Exposed accessible fluid path for no leaky surprises. Easily maintain all components

Detector adjustable wavelength 190 to 600nm

Fraction High volume fraction collection to correlate fractions with chromatogram display

Split fraction rack design allows pause-free extended runs and offline tube changes

Peak software generates an optimized purification run. Flow rates are automatically maximized whenever possible to speed purification.

Optimized conditioning with pause for sample injection

Modify the method Pause and resume the run as necessary.

Collect mode Peak, volume, windows

Minimize Method Development Time and Maximize Results!

complete most purification within 20 minutes

Features & Capabilities

Best Value




-0.1

0.0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

1.0

1.1

1.2

0 2 4 6 8 10 12 14 16 18 20

5

10

20

30

40

50

60

70

80

90

105

Time(min)



Purific

atio

n

126

Specification

Column System

Flow Pump

UV Detector

Fraction Collector

Peak Software

General

Feature/Specification

Purification Channels

Cartridge Sizes

Solid Sample Capacity

Liquid Sample Injection

Solvent Delivery

Flow rate

Gradient (# of solvents)

System Max Pressure

UV Detection

Fraction Tube Sizes

Rack Design

Architecture

Input

Video

Ports

Chromatogram display

Off-line Run Review

Auto Configuration

Sample ID

File system

Power

Dimensions HxWxD (cm)

Warranty

Description

1

4-330g

5mg-33g using prepacked SPE-type barrels or self-loaded cartridges

5-35mL, luer taper syringe port

Patented valveless metering pump

5-100 mL/min. (±1% accuracy), 50-500 mL/min optional

Binary (2) standard (one optional)

100 psi (7bar) included software low / high cut-off alarm

Field changeable filters for 190-600nm wavelength

13, 15, 18, 24mm tube racks, scintillation vials, funnels

Two rack design for extended run without pause and offline tube changes

Touch Screen

Touch Screen

10.4" TFT, solvent resistant

USB,RS-485

Real-time color , with historical pan/zoom/scroll by time & AU

Open saved run information for off-line analysis on the touch screen

"Wizard" guided setup with manual override for advanced users

Sample ID identifies samples in saved run data

Standard Windows with extended file and folder names

100-240V 50/60 Hz

60 x 48 x 48cm( 24" x 19" x 19")

One year limited warranty (ask sales representative for details)

Agela FS-2100 Flash Chromatography Workstation

GC ColumnsDA-1

DA-1MS

DA-5

DA-5MS

DA-35MS

DA-1701

DA-CarBoWax 20M

DA-FFAP

DA-INOWAX

DA-50+MS

DA-1301

DA-624

DA-PLOT Al2O

3 “KCl”

DA-PLOT Al2O

3 “S”

DA-PLOT Al2O

3 “M”

DA-PLOT MoleSieve

DA-PLOT GasPro

DA-PLOT Q

DA-PLOT U

DA-Blood Alcohol Column

128

129

130

131

132

132

133

133

134

135

136

137

138

139

140

141

142

143

144

144

AgelaTechnologies


GC

Colu

mns

128

DA-1

100% bonded and crosslinked dimethylpolysiloxane

Non-polar, equivalent to USP phase G2

Solvent rinseable and high temperature limit

Wide range of column dimensions and stationary phase film thicknesses

Low column bleed, inert surface and high efficiency

Most polular for general applications

Similar Phases: DB-1, HP-1, Ultra-1, SPB-1, RTX-1, CP-Si 5CB, BP-1, ZB-1, AT-1, MDN-1, OV-1, OV101

Length(m)

15

15

15

30

30

30

60

60

100

15

15

25

25

25

30

30

30

30

30

30

50

50

50

60

60

60

5

5

5

7.5

10

10

15

15

15

15

30

30

30

30

30

60

Cat.No.

0125-1501

0125-1502

0125-1510

0125-3001

0125-3002

0125-3010

0125-6002

0125-6010

0125-0010

0132-1502

0132-1510

0132-2501

0132-2505

0132-2510

0132-3001

0132-3002

0132-3010

0132-3030

0132-3040

0132-3050

0132-5001

0132-5005

0132-5010

0132-6002

0132-6010

0132-6050

0153-0501

0153-0508

0153-0526

0153-0750

0153-1008

0153-1026

0153-1501

0153-1515

0153-1530

0153-1550

0153-3008

0153-3015

0153-3026

0153-3030

0153-3050

0153-6050

Film(μm)

0.1

0.25

1

0.1

0.25

1

0.25

1

0.5

0.25

1

0.17

0.52

1.05

0.1

0.25

1

3

4

5

0.17

0.52

1.05

0.25

1

5

0.15

0.88

2.65

5

0.88

2.65

0.15

1.5

3

5

0.88

1.5

2.65

3

5

5

Temperature Limit(OC)

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/280

60 to 325/280

60 to 325/280

60 to 325/350

60 to 325/350

60 to 300/320

60 to 325/350

60 to 300/320

60 to 260/280

60 to 325/360

60 to 325/360

60 to 260/280

60 to 260/280

60 to 300/320

60 to 260/280

60 to 320/360

60 to 300/320

60 to 260/280

60 to 260/280

60 to 300/320

60 to 300/320

60 to 260/280

60 to 260/280

60 to 260/280

60 to 260/280

ID(mm)

0.25

0.25

0.25

0.25

0.25

0.25

0.25

0.25

0.25

0.32

0.32

0.32

0.32

0.32

0.32

0.32

0.32

0.32

0.32

0.32

0.32

0.32

0.32

0.32

0.32

0.32

0.53

0.53

0.53

0.53

0.53

0.53

0.53

0.53

0.53

0.53

0.53

0.53

0.53

0.53

0.53

0.53


Best Value





GC

Colu

mns

129

DA-1MS

Identical selectivity to DA-1,but with much lower column bleed

Ideal for GC-MS analysis and first choice of new method development

Similar phases: HP 1MS,DB 1MS,BPX-1,AT 1MS,ZB-1,Rtx-1,CP-Sil 5CB

Length(m)

15

30

30

30

30

60

15

25

30

30

30

60

Cat.No.

1125-1502

1125-3001

1125-3002

1125-3005

1125-3010

1125-6002

1132-1502

1132-2505

1132-3001

1132-3002

1132-3010

1132-6002

Film(μm)

0.25

0.1

0.25

0.5

1

0.25

0.25

0.52

0.1

0.25

1

0.25


60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

ID(mm)

0.25

0.25

0.25

0.25

0.25

0.25

0.32

0.32

0.32

0.32

0.32

0.32


AgelaTechnologies


GC

Colu

mns

130

DA-5

5% phenyl, 95% methylpolysiloxane, bonded and crosslinked

Non-polar, equivalent to USP phase G27

Solvent rinseable and high temperature limit

Wide range of column dimensions and stationary phase film thicknesses

Low column bleed, inert surface with neutral surface and high efficiency

Most popular for general applications

Similar Phases: DB-5, HP-5, Ultra-5, SPB-5, RTX-5, CP-Sil 8CB, BP-5, ZB-5, AT-5, MDN-5, OV-5, SE-52, SE-54

Length(m)

15

15

30

30

30

60

60

15

15

25

25

25

30

30

30

30

50

50

50

60

60

10

15

15

30

30

30

30

Cat.No.

0525-1502

0525-1510

0525-3001

0525-3002

0525-3010

0525-6002

0525-6010

0532-1502

0532-1510

0532-2501

0532-2505

0532-2510

0532-3001

0532-3002

0532-3005

0532-3010

0532-5001

0532-5005

0532-5010

0532-6002

0532-6010

0553-1026

0553-1515

0553-1550

0553-3008

0553-3015

0553-3026

0553-3050

Film(μm)

0.25

1

0.1

0.25

1

0.25

1

0.25

1

0.17

0.52

1.05

0.1

0.25

0.5

1

0.17

0.52

1.05

0.25

1

2.65

1.5

5

0.88

1.5

2.65

5


60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 260/280

60 to 260/280

60 to 300/320

60 to 260/280

60 to 300/320

60 to 300/325

60 to 260/280

60 to 260/280

ID(mm)

0.25

0.25

0.25

0.25

0.25

0.25

0.25

0.32

0.32

0.32

0.32

0.32

0.32

0.32

0.32

0.32

0.32

0.32

0.32

0.32

0.32

0.53

0.53

0.53

0.53

0.53

0.53

0.53


GC

Colu

mns

131

Identical selectivity to DB-5, but with much lower column bleed

Best surface inertness for wide range of compounds

Ideal for GC-MS analysis or new method development

Similar Phases: HP-5MS, DB-5MS, BPX-5MS, AT-5MS, Rtx-5MS, CP-Sil 8 CB

Length(m)

15

15

15

15

30

30

30

30

60

60

60

60

15

15

15

15

25

30

30

30

30

60

60

60

60

Cat.No.

1525-1501

1525-1502

1525-1505

1525-1510

1525-3001

1525-3002

1525-3005

1525-3010

1525-6001

1525-6002

1525-6005

1525-6010

1532-1501

1532-1502

1532-1505

1532-1510

1532-2505

1532-3001

1532-3002

1532-3005

1532-3010

1532-6001

1532-6002

1532-6005

1532-6010

Film(μm)

0.1

0.25

0.5

1

0.1

0.25

0.5

1

0.1

0.25

0.5

1

0.1

0.25

0.5

1

0.52

0.1

0.25

0.5

1

0.1

0.25

0.5

1


60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

60 to 325/350

ID(mm)

0.25

0.25

0.25

0.25

0.25

0.25

0.25

0.25

0.25

0.25

0.25

0.25

0.32

0.32

0.32

0.32

0.32

0.32

0.32

0.32

0.32

0.32

0.32

0.32

0.32


DA-5MS

Best Value





AgelaTechnologies


GC

Colu

mns

132

DA-35MS

Bonded and 35% phenyl backbone 65% dimethylsiloxane phase

Equivalent to USP phase G-42

Inert to many active compounds

Low column bleed to improve detection sensitivity and quantitation

Applications: Acroclors, PCBs, amines, pesticides, pharmaceutical molecules.

Similar Phases: HP-35MS, DB-35, BPX-35, AT-35, Rtx-35, SPB-35

Length(m)

15

30

30

60

15

30

60

15

30

30

Cat.No.

3525-1502

3525-3001

3525-3002

3525-6002

3532-1502

3532-3002

3532-6002

3553-1510

3553-3005

3553-3010

Film(μm)

0.25

0.15

0.25

0.25

0.25

0.25

0.25

1

0.5

1


40 to 320/340

40 to 320/340

40 to 320/340

40 to 320/340

40 to 320/340

40 to 320/340

40 to 320/340

40 to 300/320

40 to 300/320

40 to 300/320

ID(mm)

0.25

0.25

0.25

0.25

0.32

0.32

0.32

0.53

0.53

0.53


Bonded and crosslingked 14% Cyanopropyl-phenyl 86% dimethylsiloxane phase

Low to mid polarity


Applications: pesticides, herbiciders, halogenates. Good confirmation of analysis of active compounds on DA 5MS columns.

Similar phases: DB-1701, ZB-1701, BP-10, Rtx-1701, CP-Sil 19 CB

Length(m)

15

15

15

30

30

30

60

60

60

15

Cat.No.

6125-1501

6125-1502

6125-1510

6125-3001

6125-3002

6125-3010

6125-6001

6125-6002

6125-6010

6125-1501

Film(μm)

0.15

0.25

1

0.15

0.25

1

0.15

0.25

1

0.15


20 to 280/300

20 to 280/300

20 to 280/300

20 to 280/300

20 to 280/300

20 to 280/300

20 to 280/300

20 to 280/300

20 to 280/300

20 to 280/300

ID(mm)

0.25

0.25

0.25

0.25

0.25

0.25

0.25

0.25

0.25

0.32


DA-1701

GC

Colu

mns

133

DA-FFAP

Nitroterephthalic acid modified Polyethylene glycol (PEG)

Bonded and crosslinked

Non-alcohol/aqueous solvent rinseable

High temperature limit

Polar phase equivalent to USP phase G35

Designed for the analysis of volatitle fatty acids and phenols

Simlar Phases: HP-FFAP, DB-FFAP, CB-WAX58 (FFAP) CB, Stabilwax-DA, BP-21, 007-CW, AT-100

Length(m)

15

30

15

25

30

50

10

15

30

Cat.No.

2125-1502

2125-3002

2132-1502

2132-2505

2132-3002

2132-5005

2153-1010

2153-1510

2153-3010

Film(μm)

0.25

0.25

0.25

0.5

0.25

0.5

1

1

1


60 to 240/250

60 to 240/250

60 to 240/250

60 to 240/250

60 to 240/250

60 to 240/250

60 to 240

60 to 240

60 to 240

ID(mm)

0.25

0.25

0.32

0.32

0.32

0.32

0.53

0.53

0.53


DA-CarBoWax 20M

Polyethylene glycol (PEG), MW 20,000

Non-Bonded non-crosslinked phase

Polar phase, equivalent to USP phase G16

Replacement of packed columns with Carbowax 20M modified

Length(m)

25

50

30

25

50

10

30

Cat.No.

2220-2501

2220-5001

2225-3002

2232-2503

2232-5003

2253-1013

2253-3013

Film(μm)

0.1

0.1

0.25

0.3

0.3

1.33

1.33

Temperature Limit(OC)ID(mm)

0.2

0.2

0.25

0.32

0.32

0.53

0.53


Best Value





AgelaTechnologies


GC

Colu

mns

134

DA-INOWAX

Bonded and crosslinked Polyethylene glycol (PEG)

Solvent washable

Highest temperature limits of bonded PEG phases

Polar phase, equivalent to USP phase G16

Wide applications of polar solvent analysis in food, pharma., cosmetics, petrochemical industries

Similar Phases: HP-INNOWax, DB-WAX, DB-WAXetr, SupelcoWAX 10, CP-WAX 52 CB, CB-WAX, Stablilwax, BP-20, 007-CW, AT-Wax,

Rtx-Wax, ZB-Wax, HP-20M

Length(m)

25

25

50

50

15

15

30

30

30

60

60

60

15

15

30

30

30

60

60

60

15

30

60

Cat.No.

2020-2502

2020-2504

2020-5002

2020-5004

2025-1502

2025-1505

2025-3001

2025-3002

2025-3005

2025-6001

2025-6002

2025-6005

2032-1502

2032-1505

2032-3001

2032-3002

2032-3005

2032-6001

2032-6002

2032-6005

2053-1510

2053-3010

2053-6010

Film(μm)

0.2

0.4

0.2

0.4

0.25

0.5

0.15

0.25

0.5

0.15

0.25

0.5

0.25

0.5

0.15

0.25

0.5

0.15

0.25

0.5

1

1

1


0.2

0.2

0.2

0.2

0.25

0.25

0.25

0.25

0.25

0.25

0.25

0.25

0.32

0.32

0.32

0.32

0.32

0.32

0.32

0.32

0.53

0.53

0.53


GC

Colu

mns

135

Length(m)

12

12

25

25

50

15

15

15

30

30

30

60

15

15

15

30

30

30

60

60

15

30

30

Cat.No.

5020-1201

5020-1203

5020-2501

5020-2503

5020-5003

5025-1501

5025-1502

5025-1505

5025-3001

5025-3002

5025-3005

5025-6002

5032-1501

5032-1502

5032-1505

5032-3001

5032-3002

5032-3005

5032-6002

5032-6005

5053-1510

5053-3005

5053-3010

Film(μm)

0.16

0.31

0.16

0.31

0.31

0.15

0.25

0.5

0.15

0.25

0.5

0.25

0.15

0.25

0.5

0.15

0.25

0.5

0.25

0.5

1

0.5

1


0.2

0.2

0.2

0.2

0.2

0.25

0.25

0.25

0.25

0.25

0.25

0.25

0.32

0.32

0.32

0.32

0.32

0.32

0.32

0.32

0.53

0.53

0.53

DA-50+MS

Bonded and crosslinked 50% phenyl backbone 50% dimethylsiloxane phase

Equivalent to USP phase G-3


Low column bleed to improve detection sensitivity and quantization

Applications: Common drug screens, phenols, PAHs, free steroids, pesticides, herbicides. Good confirmation of the analysis of active

compounds on AB-5MS or AB-35MS columns

Similar Phases: HP-50+, DB -7, DB-17MS, BPX-50, AT-50, Rtx-50, SPB-50


Best Value





AgelaTechnologies


GC

Colu

mns

136

DA-1301

Bonded and crosslinked 6% Cyanopropyl-phenyl 94% dimethylsiloxane phase

Equivalent to USP phase G-43, low to mid polarity


Applications: pesticides, herbiciders, halogenates. Good confirmation of the analysis of active compounds on DA-5MS or DA-35MS columns

Similar Phases: HP-1301, DB-1301, BP-10, Rtx-1301

Length(m)

15

15

30

30

60

60

15

15

30

30

60

60

15

30

30

Cat.No.

6025-1502

6025-1510

6025-3002

6025-3010

6025-6002

6025-6010

6032-1502

6032-1510

6032-3002

6032-3010

6032-6002

6032-6010

6053-1510

6053-3010

6053-3015

Film(μm)

0.25

1

0.25

1

0.25

1

0.25

1

0.25

1

0.25

1

1

1

1.5


0.25

0.25

0.25

0.25

0.25

0.25

0.32

0.32

0.32

0.32

0.32

0.32

0.53

0.53

0.53


GC

Colu

mns

137

DA-624

Bonded and crosslinked specially designed stationary phase for EPA methods

Mid-polarity


Applications: EPA methods: 501.3, 502.2, 503.1, 524.2, 601, 602, 8010, 8015, 8020, 8240,8260

Similar Phases: DB-624, ZB-624, AT-624, Rtx-624, CP-624

Length(m)

25

30

60

30

60

30

60

75

Cat.No.

6220-2511

6225-3014

6225-6014

6232-3018

6232-6018

6253-3030

6253-6030

6253-7530

Film(μm)

1.12

1.4

1.4

1.8

1.8

3

3

3


0.2

0.25

0.25

0.32

0.32

0.53

0.53

0.53


Best Value





AgelaTechnologies


GC

Colu

mns

138

DA-PLOT Al2O

3 “KCl”

Least selective ("polar") aluminum oxide phase

Deactivated with KCl

Good baseline separation of most C1 to C10 hydrocarbons

Olefins may co-elute with corresponding paraffin over C5

Minimum surface adsorption of dienes and halocarbons

ASTM recommended phase

Preferred choice of hydrocarbon separation

Similar Phases: HP-PLOT Al2O3/KCL, GS-Alumina/KCL, CP-Al2O3/KCl PLOT, ZB-Alumina/KCl

Length(m)

25

30

15

30

50

15

30

50

60

Cat.No.

8125-2505

8125-3005

8132-1508

8132-3008

8132-5008

8153-1515

8153-3015

8153-5015

8153-6015

Film(μm)

5

5

8

8

8

15

15

15

15


0.25

0.25

0.32

0.32

0.32

0.53

0.53

0.53

0.53


GC

Colu

mns

139

Length(m)

25

30

15

30

50

15

30

50

60

25

30

15

30

50

15

30

50

60

Cat.No.

8225-2505

8225-3005

8232-1508

8232-3008

8232-5008

8253-1515

8253-3015

8253-5015

8253-6015

8325-2505

8325-3005

8332-1508

8332-3008

8332-5008

8353-1515

8353-3015

8353-5015

8353-6015

Film(μm)

5

5

8

8

8

15

15

15

15

5

5

8

8

8

15

15

15

15


0.25

0.25

0.32

0.32

0.32

0.53

0.53

0.53

0.53

0.25

0.25

0.32

0.32

0.32

0.53

0.53

0.53

0.53


DA-PLOT Al2O3 “S”

Good selective (mid "polar") aluminum oxide phase

Deactivated with Na2SO4 salt

Excellent baseline separation of C1 to C10 hydrocarbons, excellent of C3, C4 isomer baseline separations

Less accurate quantitations of dienes and halocarbons



Similar Phases: HP-PLOT Al2O3/"S", GS-Alumina, CP-Al2O3/Na2SO4 PLOT, ZB-Alumina, RT-Alumina

Best Value





AgelaTechnologies


GC

Colu

mns

140

DA-PLOT Al2O3 “M”

Strong selective (most "polar") aluminum oxide phase

Deactivated with Na2MoO4 salt

Mostly baseline separation of C1 to C10 hydrocarbons, good baseline separation of cyclopropane from propylene

Accurate quantitations of dienes and halocarbons



Similar Phases: HP-PLOT Al2O3/"M", GS-Alumina, ZB-Alumina

Length(m)

25

30

15

30

50

15

30

50

60

Cat.No.

8325-2505

8325-3005

8332-1508

8332-3008

8332-5008

8353-1515

8353-3015

8353-5015

8353-6015

Film(μm)

5

5

8

8

8

15

15

15

15


0.25

0.25

0.32

0.32

0.32

0.53

0.53

0.53

0.53


GC

Colu

mns

141

Length(m)

5

15

30

15

30

5

15

30

15

30

Cat.No.

8432-0512

8432-1512

8432-3012

8432-1525

8432-3025

8453-0525

8453-1525

8453-3025

8453-1550

8453-3050

Film(μm)

12

12

12

25

25

25

25

25

50

50


0.32

0.32

0.32

0.32

0.32

0.53

0.53

0.53

0.53

0.53


DA-PLOT MoleSieve

Molecular Sieve, Zeolite, 5A

Thin and thick coatings for push limit separations, e.g. Ar/O2 separation at 35C, O2/N2 with 15sec

Truly immobilized coating and smooth baseline workable for valve switching

Preferred column to analyze fixed gases from hydrocarbon streams

Elution order, H2, He, Ne, O2, N2, CH4, CO - Regeneration of performance from water/CO2 adsorptions at 250 for 3hr


Noble gas, air from methane, fuel cell, etc

Often used with other PLOT columns for fixed gas separation from sample matrix

Similar Phases: HP-PLOT Molesieve, CP-PLOT MoleSieve.



Best Value



AgelaTechnologies


GC

Colu

mns

142

DA-PLOT GasPro

Proprietary Silica base phase

Very inert to wide range of gases including sulfur gases and Freons, symmetric peak shapes

Truly immobilized coating and smooth baseline workable for valve switching

Preferred column to analyze sulfur gases from hydrocarbon streams and air

Separation of CO and CO2

No effect of water on retention time

Regeneration of performance from water adsorptions at 250 for 3hr


GC-MS applicable

Replacement: GS-GasPro, CP-Silica PLOT

Length(m)

5

15

30

Cat.No.

8532-0505

8532-1505

8532-3005

Film(μm)

5

5

5


0.32

0.32

0.32


GC

Colu

mns

143

Length(m)

15

30

15

30

Cat.No.

8632-1515

8632-3015

8653-1530

8653-3030

Film(μm)

15

15

30

30


0.32

0.32

0.53

0.53


DA-PLOT Q

Divinylbenzene polymer particle, non-polar, close to traditional PoraPak Q

Truly immobilized coating and smooth baseline workable for valve switching and liquid sample introduction at low temperatures

Highest temperature limit, up to 280/300

Baseline separation of Air, CO2, water, sulfur gases, hydrogen chlorine, ammonia/light amines from hydrocarbons such as refinery

stream, natural gas, and engine exhaust

Excellent separation of C1 to C3 isomers, good separations of C3 to C12, halocarbons

Excellent inertness and long lifetime of water/alcohol sample, a good secondary choice of wax column for alcohol analysis

Excellent tolerance to strong acid/base sample without affecting retention time

Good anti-oxidation at 280 and fast regeneration of performance

Observed slight sulfur gas, hydrogen chlorine, and amine absorption

Water peak is tailing, but no effect on retention

Good choice for analyzing low level solvent residues from wide samples, such as headspace, aqueous, or organic solutions

Preferred column for residue analysis in petroleum industry, pharmaceutical, environmental, chemical weapon detection

Preferred column to analyze polar compounds including oxygenates from hydrocarbon streams: natural gas, refinery gas, dienes,

ethylene, propylene

Replacement: HP-PLOT Q, GS-Q, CP-PoraPLOT Q HT, Supel PLOT Q, Rt-Q, ZB-PLOT Q

Best Value





AgelaTechnologies


GC

Colu

mns

144

DA-PLOT U

Divinylbenzene and Ethylene glycol dimethacrylate copolymer particle, polar, close to traditional PoraPack U

Truly immobilized coating and smooth baseline workable for valve switching and liquid sample introduction at low temperatures

Improved separation and selectivity of Air, CO2, water, sulfur gases, hydrogen chlorine, ammonia/light amines from hydrocarbons

Symmetric water peak shape, water elute after C3

Excellent separation of C1 to C3 isomers, good separations of C3 to C12, halocarbons

Excellent inertness and long lifetime of water/alcohol sample, a good secondary choice of wax column for alcohol analysis

Excellent tolerance to strong acid/base sample without affecting retention time

Observed slight sulfur gas, hydrogen chlorine, and amine absorption

Good choice for analyzing low level solvent residues from wide samples, such as headspace, aqueous, or organic solutions

Preferred column for residue analysis in petroleum industry, pharmaceutical, environmental, chemical weapon detection

Preferred column to analyze polar compounds including oxygenates from hydrocarbon streams: natural gas, refinery gas, dienes,

ethylene, propylene

Replacement: HP-PLOT U, CP-PoraPLOT U

Length(m)

7.5

Cat.No.

8932-0710

Film(μm)

10


0.32


DA-Blood Alcohol Column

Proprietary PLOT phase

Reliable blood alcohol analysis

Tolerance of direct sample injection

Excellent confirmation column to other blood alcohol columns using t-butanol as internal standard

Length(m)

15

30

30

15

30

Cat.No.

8732-1510

8732-3010

8732-3020

8753-1520

8753-3020

Film(μm)

10

10

20

20

20


0.32

0.32

0.32

0.53

0.53



Column Savor –Online Filter

HPLC Mixer

HPLC Fittings and Tubing

HPLC Injector and Sample Loop

Automated Switching Valve

Automatic Column Selector

HPLC Column Heater

UV Lamps


SPE Vacuum Manifolds

Multipurpose SPE Work-station

SPE-1 Automated Processing Station

Positive Pressure SPE Work-station

Robotic SPE Work-station

Solvent Evaporator

Empty Column and Accessories

Pipettes and Accessories


Septa

Injection Port Liners

Ferrules

Column Scribe

1/32” Ultra Low Mass External Unions

Filtration Products

Syringe Filters

96 well filtration plates and collection plates

Membrane Filters

Glass Filtration System

Chemical Resistance Guide for Membranes

Vials and Caps

Screw Neck Vials and Caps

Crimp Neck Vials and Caps

Plastic Vials (Micro-vials for LC-MS Autosamplers)

146

146

146

147

149

149

150

150

151

153

153

153

154

154

154

154

155

156

157

157

157

158

161

161

162

162

164

164

164

165

166

166

166

166

Equipments, Accessories and

SuppliesAccessories andAccessories andAccessories andAccessories andAccessories andAccessories andAccessories andAccessories andAccessories andAccessories andAccessories andAccessories andAccessories andAccessories andAccessories andAccessories andAccessories andAccessories andAccessories andAccessories andAccessories and





Supplies


Supplies


Supplies


Supplies


Supplies


Supplies


Supplies


Supplies


Supplies


Supplies


Supplies


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Supplies


Supplies


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Supplies


Supplies


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AgelaTechnologies


Accessories/S

upplie

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146

Column Savor –Online Filter


Online Filters

Description

PEEK, ZDV

Cat.No.

OFP-001


Bottom-of-the-Bottle Solvent Filters

10um Filter Assembly¹,for 1/8˝ OD tubing with A•520 replaceable filter cup

2um Filter Assembly¹,for 1/8˝ OD tubing with A•522 replaceable filter cup

10um Filter Assembly²,for 3/16˝ OD tubing with A•520 replaceable filter cup

2um Filter Assembly²,for 3/16˝ OD tubing with A•522 replaceable filter cup

Replacement Filter Cups (For AA•550-AA•553 Filters)

10um Stainless Steel Cups,10-pk

2um Stainless Steel Cups,10-pk

Inlet Solvent Filters

Totally PEEK polymer wetted surfaces

Biocompatible and inert

For analytical systems

High-pressure applications

HPLC Mixer

Static Bed Mixers

Mixer cartridge Vol. Applicable Flowrate(µL) Cat.No.

50

150

250

20-150

150-500

500

SMX050

SMX150

SMX250


Cat.No.

AA•550

AA•551

AA•552

AA•553

AA•520x

AA•522x

Accessories/S

upplie

s

147

Cat.No. Tubing Size /Nut Threads QtyMaterial

Kel-F

PEEK

PEEK

PEEK

PEEK/Kel F

Kel F

AF 100

AF 120

AF 127

AF 130

AM 645

AP 100

1/16”, 10 • 32

1/16”, 10 • 32

1/16”, 10 • 32

1/16”, 10 • 32

1/32”, 6 • 40

1/8”, 1/4 • 28

10-pk

10-pk

10-pk

10-pk

10-pk

each

Cat.No. Tubing Size /Nut Threads QtyMaterial

Delrin

PEEK

Long PEEK

Short PEEK

Delrin

PEEK

Long PEEK

Short PEEK

AF 200

AF 300

AF 330

AF 331

AF 200n

AF 300n

AF 330n

AF 331n

1/16”OD, 10 • 32

1/16”OD, 10 • 32

1/16”OD, 10 • 32

1/16”OD, 10 • 32

1/32”OD, 10 • 32

1/32”OD, 10 • 32

1/32”OD, 10 • 32

1/32”OD, 10 • 32

10-pk

10-pk

10-pk

10-pk

ea

ea

ea

ea

Two-piece Fingertight Fittings

AF-120 AF-130 AF-127

AF-300 AF-330 AF-331 AF-200

One-piece Fingertight Fittings

Unions

Material

PEEK ZDV

Stainless Steel ZDV

Material

PEEK

Stainless Steel

Type

High Pressure, webbed

High Pressure, webbed

Type

High Pressure

High Pressure

OD

1/16"

1/16"

OD

1/16"

1/16"

Cat.No.

Cat.No.

TEP-001

TES-001

Tees

Connections (Unions and Tees)

HPLC Fittings and Tubing

All of One-piece Fingertight Fittings are designed to be used with 1/16” OD tubing, except the AM-645(1/32”) and AP-100(1/8”)

UNP-001

UNS-001

Best Value





AgelaTechnologies


Accessories/S

upplie

s

148

PEEK Tubing

PEEK polymer tubing is a good replacement of stainless steel tubing used commonly in HPLC systems. It has many advantages over the

stainless steel tubing, which includes better chemical inertness, better biocompatibility, and convenience to cut. Additionally, PEEK tubing has

a very smooth internal surface, which cause less turbulence than metal tubing. Unlike other polymer tubing, PEEK tubing can tolerate high

pressure (up to 7000 psi) and high temperature (up to 100 oC).

1/16” OD x 5’ L

1/8” OD x 5’ L

1.8 mm OD x 5’ L

1/32” OD x 5’ L

Cat.No.

AT1560

AT1561

AT1535

AT1562

AT1536

AT1531

AT1565

AT1532

AT1533

AT1538

AT1537

AT1534

AT1544

AT1545

AT1546

AT1547

AT1540

AT1539

AT1579

AT1578

AT1576

AT1577

AT1575

AT1580

AT1581

AT1568

AT1569

I D

0.0025˝( 65 μm )

0.004˝ (100 μm )

0.005˝ (125 μm )

0.006˝ (150 μm )

0.007˝ (175 μm )

0.010˝ (0.25mm)

0.015˝ (0.40mm)

0.020˝ (0.50mm)

0.030˝ (0.75mm)

0.040˝ (1.00mm)

0.055˝ (1.40mm)

0.062˝ (1.60mm)

0.080˝ (2.00mm)

0.010˝ (0.25mm)

0.020˝ (0.50mm)

0.030˝ (0.75mm)

0.042˝ (1.05mm)

0.055˝ (1.40mm)

0.025˝ ( 65μm )

0.035˝ ( 90μm )

0.050˝ ( 125μm )

0.070˝ ( 175μm )

0.080˝ ( 200μm )

0.090˝ ( 230μm )

0.100˝ (0.25mm)

0.150˝ (0.40mm)

0.200˝ (0.50mm)

Pressure Rating

7,000psi (483bar)*

7,000psi (483bar)*

7,000psi (483bar)*

7,000psi (483bar)*

7,000psi (483bar)*

7,000psi (483bar)*

7,000psi (483bar)*

7,000psi (483bar)*

7,000psi (483bar)*

5,000psi (345bar)*

500psi ( 34bar)*

4,000psi ( 276bar)*

3,000psi ( 207bar)*

7,000psi (483bar)*

7,000psi (483bar)*

7,000psi (483bar)*

5,000psi (345bar)*

500psi ( 34bar)*

5,000psi (345bar)*

5,000psi (345bar)*

5,000psi (345bar)*

5,000psi (345bar)*

5,000psi (345bar)*

5,000psi (345bar)*

5,000psi (345bar)*

5,000psi (345bar)*

5,000psi (345bar)*

Tubing Holders

Tubing Holders For Polymer Tubing

AP•740

AP•741

AP•815

90°Tubing Holder for 1/16˝ OD tubing, polystyrene

180°Tubing Holder for 1/16˝ OD tubing, polystyrene

90°Tubing Holder for 1/8˝ - 5/16˝ OD tubing, PVT

Cat.No.

Accessories/S

upplie

s

149

Tubing Cutters

Tubing Cutters For Polymer Tubing

AA•327

AA•329

AA•350

AA•328

AF•262x

AF•264x

AF•267Bx

AFS•315

AFS•315•02

Standard Polymer Tubing Cutter* for 1/16˝ and 1/8˝ OD tubing

Large Bore Polymer Tubing Cutter* for 3/16˝ - 5/16˝ OD tubing

Capillary Polymer Tubing Cutter* for 360μm- 1/32˝ OD tubing Includes(1)F•262x10-pack of sleeves

Replacement Blades for A •327, A •329and A •350 5-pk

Replacement Sleeves for A •350,0.0155˝ID,Green, for cutting 360μm OD tubing 10-pk

Alternate Sleeves for A •350,0.021˝ID,Natural, for cutting 510μm OD tubing 10-pk

Alternate Sleeves for A •350,0.033˝ID,Blue, for cutting 1/32˝ OD tubing 10-pk

Precision Fused Silica Tubing Cutter

Maintenance Kit for FS•315

HPLC Injector and Sample Loop

Sample Injector

Type Description Cat.No.

Rheodyne 7725 Injector



Standard Analytical Sample Injector; Stainless steel

Standard Analytical Sample Injector; Peek

Preparative Sample Injector

INJ7725

INJ9725

INJ3725

Sample Loop

Type Cat.No.

5µL Stainless Steel

5µL Peek


20µL Peek


50µL Peek


100µL Peek

1mL Stainless Steel

1mL Peek

5mL Stainless Steel

10mL Stainless Steel

LPS0005SS

LPS0005PK

LPS0020SS

LPS0020PK

LPS0050SS

LPS0050PK

LPS0100SS

LPS0100PK

LPS1000SS

LPS1000PK

LPS5000SS

LPS0000SS

Automated Switching Valve

Used for 2D-LC or on-line SPE/LC

Editable valve diagram.

Flexible in choosing valve number and valve type.

The valve types can be 2-position/6-port; 2-position/8-port; 2-position/10- port; 6-position

or 10 position.

One module can be installed with 1 or 2 valves. Two modules can be used together to

make a 4-valve platform or be sued as two independent modules.


Type Cat.No.

LC-04SP Valve System ASV0002-0

Cat.No.

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HPLC Column Heater

Cat.No.

Dimensions (H×W×D)

Operating Temperature

Temperature Accuracy

Compatible Column Quantity and Length

Power

Weight

CC-100

510mm×110mm×65mm

Above 5 100

±0.1

2×300mm

220V/50Hz/1A

4Kg

Technical specification

Automatic Column Selector

Connect up to 6 HPLC columns

Controllable by both panel keys and computer

Easy key operation for setting method and sequence

Compact design and easy access to columns

Compatible with any HPLC

Compatible with other LC-04SP valves

Cat.No.: AST-006

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UV LampsAgela Technologies offers UV lamps of the same or better quality as a low cost replacement for many of those manufactured by original HPLC

manufacturers, such as Waters, Agilent, Varian, Shimazu and Perkin-Elmer.

Original Instrument Part Number Agela Lamps Part Number Type of Lamps

AL 610

ALH 10

AL 890

AL 610

AL 295

ALH 10

ALH l0

ALH l6

ALH 855

ALH 11

ALH 12

ALH 15

ALH 13X2

AL 850

ALH 10

ALH 14

ALH 13

ALH 13X2

AL 850

ALH l4

ALH 10

W

D2

P/Xe

W

D2

D2

D2

D2

W

D2

D2

D2

D2

mf

D2

D2

D2

D2

W

P/Xe

W

D2

D2

l037

l040A,1040M

1046

l047A

79853A

79853C,79854A

79875A

79880A,7988lA

8450A

8450A

8451A

8452A

8453A,8453E

8453A

8453A

G1306A

G13l4A

Gl315A&B,Gl365A&B

Gl315A&B,Gl365A&B

Gl3l5A&B,Gl365A&B

Gl32l A

Gl362

HP3D/CE

Series I&II

Agilent


AL 305

ALH 80

AL 830

ALH 81

AL 935

AL 830

ALH 80

AL 835

D2

D2

W

D2

W

W

D2

W

SPD-7A, 7AV

SPD-10A, 10 AV, 10AVP

SPD-10 AV, 10AVP

SPD-M1A

SPD-M6A, M10A, M10AV

SPD-M6A, M10A

SPD-M10AV

SPD-M10AVP

SPD-M10AVP

Shimadzu

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AL 230

AL 230

AL 230

AL 230

AL 230

ALH 910

ALH 80

ALH 80

ALH 950

AL 305

ALH 950

ALH 53

ALH 40

ALH 910

AL 800

AL 230

AL 305

ALH 950

AL 305

AL 305

D2

D2

D2

D2

D2

P/Xe

P/Xe

D2

D2

W

P/Xe

D2

P/Xe

D2

D2

P/Xc

Xe

P/Xe

D2

D2

P/Xe

D2

D2

AA200,275,300

AA400,600,640

AA775,800,875

AA975,l275,l475

AA3000

DMS90

Eclipse

FL-l,2

LC-5000,5500

PDA,1

PDA-2

PDA-2

Pro Star

pro Star 310

Pro Star 320

Pro Star 330

Pro Star 340,345

Pro Star 360

Pro Star 363

Pro Star 364

Spectra A10,20,30,40

Star 9050

1JV•1,lA

UV50,100,200

VIsta 5500

Varian


AL 770

AL 820

AL 720

AL 7l5

ALHA l

ALH l

AL 800

AL 205

ALH 3O

ALH 3l

ALHA 34

ALH 34

ALH 33

ALH 925

ALH 36X2

ALH 33

ALH 3l

Hg

Hg

Cd

Zn

D2 Lamp Ass’y

D2 Replacement

Xe

D2

D2

D2

D2 Lamp Ass’y

D2 Replacement

D2

Xe

D2

D2

D2

40l,403,404

420

440,44l

441

441

450

450

470,474

480,48l

484

486

990,99l,994

990,991,994

996

2475

2487,2488

2996

LC Module l/3455l System

Waters

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SPE Vacuum Manifolds


Type

12 port Model

24 port Model

96well plate Model

Cat.No.

VM0012

VM0024

VM0096


Multipurpose SPE Work-station

SPE Vaccum Manifold for SPE sample preparation, filtration and elution are available in 12

and 24 port configurations. These manifolds permit consistent extraction and filtration results.

Multiple sample processing with these manifolds consist of a clear glass chamber and lid, to

which a vacuum is applied to draw solvents and sample through an SPE column, cartridge,

or disk. The lid is CNC machined, solvent resistant, low extractable virgin polypropylene. The

lid is autoclavable, and will not wrap. The female Lure inlets and male Lure outlets are

molded of pure polypropylene. Adjustable racks placed in the glass chamber will accommo-

date a variety of sample collection vessels, including test tubes, autosampler vials, volumet-

ric flasks, and Erlenmeyer flasks. Eluants are deposited directly into the collection vessel of

choice via polypropylene, or optional stailness steel or Teflon needles.

Ease for operation: easier to change the collection tubes

Flexibility: can be used for vacuum or positive pressure mode;

more choices for tube size

Multifunction: SPE processing and solvent evaporation

Solid: made of aluminum alloy instead of fragile glass

Compatible with automated liquid handling systems

Cat.No.: MST-001

SPE-1 Automated Processing Station

Designed for sample preparation in trace analysis of food and environmental samples.

Process up to 6 samples per batch without attendance.

Up to 5 solvents can be used for column conditioning and multi step elution.

Two fractions can be collected for each sample.

Accommodate SPE cartridges and traditional cleanup columns.

Exhaustive sample injection.

Easy operation with only 7 buttons.

Small footprint and computer-free operation

Cat.No.: APT-001

This is specially designed to replace conventional SPE manifolds

and solvent evaporators by an integrated and versatile workstation.

It features:

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Cat.No.

APSP-48

APSP-22

APSP-23

APSP-24

APSP-19

APSP-20

APSP-21

APSP-25

APSP-26

APSP-27

APSP-28

Description

Positive Pressure Processor-48 Position

Rack for 1 ml SPE Columns



Collection Tube Rack, 12 x 75 mm Tubes



Collection Vial Rack, 12 x 32 mm Auto-Sampler

Waste Bin

48-Column Sealing Gasket

Gas Supply Adapter

Qty/Pk

1

1

1

1

1

1

1

1

1

1

1


Positive Pressure SPE Work-station

48 position

96 position

Robotic SPE Work-station

Cat.No.: RST-0096

24 well to 96 well reformatting

96 and 384 well dispensing

Automated Tip changing

96 well and 384 well liquid level sensing

12 decks for large sample processing

Product Features

Solvent Evaporator

Combination of heating and vacuum for speedy evaporation of a variety of solvents at relative lower temperature (safe, faster and better for

unstable compounds)

Simple operation, no programming set-up needed

Adjustable gas pressure and flow

Multi-size block interchangeable, accepts tubes OD from 11 to 29mm

Optional automatic shutoff, for safe and fast parallel enrichment of various samples

Small footprint, only 18(W)x16(D)inch bench area

Broad customization configurations

Economic and reliable

1.Height post

2.Vacuum vent

3.Needle piercing gas manifold

4.Sample tubes

5.Temperature controller

6.System flow controller

Multi-Needle manifold with individually

controlled flow

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Dimension

Weight

Power

Mount

Working environment

Well plate dimension

Number of Well Plate

Tube OD

Tube length

Temperature

Temperature accuracy

Gas pressure

Gas flow

Needle

Gas/vacuum connection fitting

Auxiliary vacuum

18(W)x20(H)x16(D) inch

45(W)x50(H)x40(D)cm

20lb/10kg

90-120VAC, 50-60Hz, 500VA

Bench stand

4 to 40°C, Humidity <80%

5(W)x4.5(D)x2(H)inch127(W)x110(D)x50(H)mm

12, 15, 30, 48

11-29mm

125-200(mm)

RT-150°C

+-1°C

30psi (2bar) max

0-5liter/min

Stainless steel, 1.6(OD)x150(L)mm

Hose barb 1/8 flow port

5-12psi, 200-700mmHg

Technical Specifications:

Empty Columns and Accessories

Description

1mL, 100/pk

3mL, 100/pk

6mL, 100/pk

1mL, 3mL, 6mL column,12/pk

Tube Part No.

050901

050903

050906

050909

Frit Part No.

050911

050913

050916


Description

Solvent evaporator, including a multi-needle manifold, inert gas flow module,

heater & controller, 120VAC, 1year limited warranty.

Order multi-well blocks and manifold plates separately!

Solvent evaporator, including individual flow controlled needle manifold,

heater & controller, 120VAC, 1year limited warranty

Order multi-well blocks and manifold plates separately!

Solvent evaporator, 48 or 96well plate format, including purge manifold,

inert gas heating & controller, 120VAC, 1year limited warranty

Cat.No.

GD-101(regular system)

GD-102-xx, xx is number of wells of block

GD-103-48

GD-103-96


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Pipettes and Accessories

Cat No.

A722001

A722004

A722010

A722015

A722020

A722025

A722030

A722035

A722040

A722045

A722050

A722055

Volume (μL)

5

10

20

25

50

100

200

250

500

1000

2000

5000

Mechanical Pipette (Fixed Volume)

Cat No.

AD781348

AD781349

AD780006

AD780007

AD780019

AD780020

AD780046

Qty

FT10μL

FT10μL

FT300μL

FT300μL

FT1000μL

FT1000μL

FT1200μL

Pipette Tips

Cat No.

8 - Channel Pipette

A720210

A720220

A720240

Volume (μL)

0.5-10

5-50

50-300

Increment (μL)

0.1

0.5

5

Cat No.

12 - Channel Pipette

A720310

A720320

A720340

Volume (μL)

0.5-10

5-50

50-300

Increment (μL)

0.1

0.5

5

Cat No.

Single Channel Pipette

A720005

A720000

A720020

A720080

A720050

A720070

A720030

A720060

A720040

A720110

Volume (μL)

0.1-2.5

0.5-10

5-50

2-20

10-100

20-200

50-200

100-1000

200-1000

1000-5000

Increment (μL)

0.05

0.1

0.5

0.5

1

1

1

5

5

50

Mechanical Pipette (AdjustableVolume)

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Septa


Septa BTO

Cat.No.

B02-1002

B02-1040

B02-1031

B02-1001

B02-1032

Description

Septa BTO 5mm Center guide (50/pk)

Septa BTO 3/8" (9.5mm) (50/pk)

Septa BTO 11.5mm Center guide (50/pk)

Septa BTO 11mm (7/16") Center guide (50/pk)

septa BOT 17mm,Center guide

Maximun temperature 400

Virtually eliminates injection-port sticking

Preconditioned packaged in glass to prevent contamination

Advanced Green 3

Cat.No.

B02-1006

B02-1005

B02-1041

Description

Septa AG3 5mm Center guide (50/pk)

Septa AG3 11mm (7/16") Center guide (50/pk)

Septa AG3 3/8" (9.5mm) (50/pk)

True low-bleed, high temperature green septum.

Each batch GC-FID tested.

Ideal for use with low-bleed, “Mass Sepc” capillary columns

For Agilent Capillary GC Instruments

Cat.No.

B02-3004

B02-3005

B02-3007

B02-3008

B02-3009

B02-3011

B02-3012

B02-3013

B02-3015

B02-3019

B02-3020

B02-3022

B02-3030

B02-3031

B02-3032

B02-3033

B02-3034

B02-3036

Description

Liner, 4 mm,79mm length, Single Taper w/ Sil Wool



Liner, 4 mm,79mm length, Double Taper



Liner, 4 mm,79mm length, Single Taper



Liner, 4mm,79mm length, with Silanized Wool



Liner, 2 mm,79mm length, Splitless, Glass



Liner, 2 mm,79mm length Splitless, Quartz



Qty.

1/pk

5/pk

10/pk

1/pk

5/pk

10/pk

1/pk

5/pk

10/pk

1/pk

5/pk

10/pk

1/pk

5/pk

10/pk

1/pk

5/pk

10/pk

Injection Port Liners

Organosilane deactivated borosilicate glass (quartz where notes). Guaranteed inertness for phenols, organic acids and bases. Glass wool is

also deactivated. These capillary injection port liners provide a clean, non-adsorptive surface for your samples. Regular replacement of the liner

protects your capillary columns from potentially harmful non-volatiles that might otherwise deposit on your columns.

Maximum temperature 400

Extended temperature range, low-bleed

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For Perkin-Elmer Capillary GCs

Cat.No.

B02-3201

B02-3202

B02-3203

B02-3204

B02-3205

B02-3206

B02-3207

B02-3208

B02-3209

B02-3210

B02-3211

B02-3212

Description

Liner, 4 mm Split, 92mm length ,Autosystem for PerkinElmer GC



Liner, 4 mm Split,92mm length Autosystem w/ Wool for PerkinElmer (ea)



Liner,2mm Splitless,92mm length, Autosystem for PerkinElmer GC



Liner,100mm, Dimple Spliter for PerkinElmer GC

SLiner,100mm, Dimple pliter for PerkinElmer GC

Liner,100mm, Dimple Spliter for PerkinElmer GC

Qty.

1/pk

5/pk

10/pk

1/pk

5/pk

10/pk

1/pk

5/pk

10/pk

1/pk

5/pk

10/pk

For Varian Capillary GCs

Cat.No.

B02-3103

B02-3104

B02-3105

B02-3112

B02-3113

B02-3114

B02-3115

B02-3116

B02-3117

B02-3118

B02-3119

B02-3120

Description

4 mm Open Split w/ Sil. Wool for Varian 1075/1077



Liner, Frit Split for Varian 1075/1077



Liner, 4 mm Open Split, for Varian 1075/1077



Liner, 74mm length, Splitless, Glass for Varian 1075/1077 (ea)



Qty.

1/pk

5/pk

10/pk

1/pk

5/pk

10/pk

1/PK

5/PK

10/PK

1/pk

5/pk

10/pk

Vespel Ferrules-Sold in Packages of 10

Cat.No.

1/16" ferrules

1/8" ferrules

1/4" ferrules

1/16" to 0.3mm ferrules






1/16" to 0.4mm -Agilent style



1/8" to 0.4mm Vespel



1/8" to 1/16" Vespel

1/4" to 1/16" ferrules

1/4" To 1/8" ferrules

Ferrules

Upper temperature limit : 350

100% high-temperature DuPont polyimide

Fairly hard; tends to seal

permanently to the column

B02-2125

B02-2133

B02-2141

B02-2126

B02-2127

B02-2128

B02-2129

B02-2130

B02-2131

B02-2012

B02-2013

B02-2014

B02-2135

B02-2136

B02-2137

B02-2138

B02-2143

B02-2144

Reducing Ferrules

Capillary Column Ferrules

Straight Ferrules-for tubing with ODs as listed

Agilent style—Short ferrule, especially for Agilent inlet systems : 4890,5890 and 6890

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Graphite Ferrules—Sold 10/pack Except as Noted

Cat.No.


B02-2101

B02-2109

B02-2116

Short Ferrules for Agilent Inlets

B02-2001

B02-2002

B02-2003

B02-2004


B02-2102

B02-2103

B02-2104

B02-2105

B02-2106

Reducing Ferrules

B02-2110

B02-2111

B02-2112

B02-2113

B02-2117

B02-2118

B02-2119

B02-2120

B02-2121

B02-2122

B02-2123

B02-2124

Description

1/16" Graphite Ferrule



1/16"to 0.4mm Graphite Ferrule









1/8" to 0.4 mm Graphite Ferrule



1/8" to 1/16" Graphite Ferrule










High-purity Graphite; Preferred for capillary GC use

Ideal for glass-to-metal connections; doesn't stick to glass

Reusable if not over-tightened

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Vespel/Graphite Ferrules –Sold in Packages of 10

Cat.No.


B02-2145

B02-2153

B02-2160

Short Ferrules for Agilent Inlet

B02-2005

B02-2006

B02-2007


B02-2146

B02-2147

B02-2148

B02-2149

B02-2150

Reducing Ferrules

B02-2154

B02-2155

B02-2156

B02-2157

B02-2161

B02-2162

B02-2163

B02-2164

B02-2166

B02-2167

Description

1/16" V/G Ferrules

1/8" V/G Ferrules

1/4" V/G

1/16" to 0.4mm V/G Ferrules


1/16" to 0.8mm V/G



1/16" to 0.8mm V/G


1/16" to 1.2mm V/G



1/8" to 0.8mm V/G

1/8" to 1/16" V/G Ferrules

1/4" to 0.4mm V/G

1/4" to 0.5mm V/G

1/4" to 6.0 mm V/G Ferrules


1/4" to 1/16" V/G Ferrules

1/4" to 1/8" V/G


Made from quality DuPont polyimide/graphite blend

Recommended for GC/MS interface applications

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Teflon Ferrules –Sold in Packages of 10

Cat.No.


B02-2169

B02-2176

B02-2182


B02-2170

B02-2171

B02-2172

B02-2173

B02-2174

B02-2175

Reducing Ferrules

B02-2177

B02-2178

B02-2179

B02-2180

B02-2183

B02-2185

B02-2186

B02-2187

Description

1/16" Teflon

1/8" Teflon

1/4" Teflon

1/16" to 0.3mm Teflon









1/8" to 1/16" Teflon

1/4" to 6mm Teflon


1/4" To 1/8" Teflon



100% Teflon ;completely inert


Cat.No.

B02-1006

Description

Column Scribe,10/pk

Column Scribe

Used to cut capillary tubing. Simply pull one smooth edge of the hard ceramic scribe (tilted about 45 degrees) across the column to lightly score

the fused silica .Bend the column opposite the score and the column separate cleanly.


Cat.No.

B02-4001

B02-4002

B02-4003

B02-4004

B02-4005

B02-4006

Description

1/32" Union,Bore 0.25mm

1/32" Union,Bore 0.50mm

1/32" Union,Bore

1/32" 1/32" Fused Silica Tubing Adapter Tubing o.d.<0.25mm

1/32" Fused Silica Tubing Adapter Tubing o.d. 0.25-0.4mm

1/32" Fused Silica Tubing Adapter Tubing o.d.0.5mm

1/32” Ultra Low Mass External Unions

For capillary tubing 0.1 to 0.32mm I.D. It has very low mass and can be hand-tightened to seal .Use only with 1/32” fused silica adapters; metal

ferrules cannot be used.

Best Value





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Syringe Filters

Filtration Products


Mixed cellulose (acetate and nitrate) syringe filters

Nylon syringe filters

13mm Syringe Filters 0.2μm 100 AS011320

0.45μm 100 AS011345


0.45μm 100 AS012545

13mm Syringe Filters with glass prefilter 0.2μm 100 AS011320-G

0.45μm 100 AS011345-G


0.45μm 100 AS012545-G


0.45μm 100 AS021345


0.45μm 100 AS022545

13mm Syringe Filters with PP prefilter 0.2μm 100 AS021320-P

0.45μm 100 AS021345-P


0.45μm 100 AS022545-P

PVDF syringe filters


0.45μm 100 AS031345


0.45μm 100 AS032545


0.45μm 100 AS031345-P


0.45μm 100 AS032545-P

PTFE syringe filters


0.45μm 100 AS041345


0.45μm 100 AS042545

1μm 100 AS042501

5μm 100 AS042505


0.45μm 100 AS041345-P


0.45μm 100 AS042545-P

1μm 100 AS042501-P

5μm 100 AS042505-P

High Quality – All of our products are manufactured by ISO 9001 registered manufacturers and are guaranteed to meet our

published specification.

Best Value – Our list prices are significantly lower than most brand name prices in the market.

Qty. Cat No.

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Polyethersulfone (PES) syringe filters

Regenerated cellulose (RC) syringe filters


0.45μm 100 AS051345


0.45μm 100 AS052545


0.45μm 100 AS051345-G


0.45μm 100 AS052545-G


0.45μm 100 AS061345


0.45μm 100 AS062545


0.45μm 100 AS061345-G


0.45μm 100 AS062545-G

The Syringe Filters’ Specifications

Description

MCM

Nylon

PVDF

PTFE

13

13

25

25

13

13

25

25

13

13

25

25

13

13

25

25

0.22

0.45

0.22

0.45

0.22

0.45

0.22

0.45

0.22

0.45

0.22

0.45

0.22

0.45

0.22

0.45

7

7

5

5

7

7

5

5

7

7

5

5

7

7

5

5

3.52

2.20

3.52

2.20

2.80

2.50

2.80

2.50

1.20

0.56

1.20

0.56

1.00

0.63

1.00

0.63

18

60

18

60

25

75

25

75

15

35

15

35

24

60

24

60

0.65

0.65

3.90

3.90

0.65

0.65

3.90

3.90

0.65

0.65

3.90

3.90

0.65

0.65

3.90

3.90

16

20

16

20

5

8

5

8

Diameter of

the filter(mm)

Pore size

(μm)

Pressure

rating(bar)

Bubble Point

rating(bar)

Water speed

(ml/min/cm2)

Air speed

(L/min/cm2)

Effective

filter area(cm2)

Best Value





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Membrane Filters

Material: cellulose acetate, nylon

Pore size: 0.45μm

Filter size: 13mm, 25mm, 50mm

Customized sizes available upon

request

Material Diameter(mm) Qty Cat. No.

13

25

50

13

25

50

Cellulose Acetate

Nylon

100/pk

100/pk

100/pk

100/pk

100/pk

100/pk

AM011345

AM012545

AM015045

AM021345

AM022545

AM025045

Glass Filtration System

Funnel Volume (mL) Flask Volume (mL) Qty Cat. No.

Ordering InformationFunnel volume: 300mL, 500mL

Pore size of the glass support: 10μm

Flask volume: 1000ml, 2000mL1000

2000

1000

2000

300

300

500

500

ea

ea

ea

ea

AM3100

AM3200

AM5100

AM5200

The system includes a borosilicate glass funnel, a sintered borosilicate glass membrane support base on neck, aluminum clamp and borosili-

cate glass filter flask. It can be used with or without membrane depending on the application requirement to filtrate organic or aqueous solu-

tion. It is also used for liquid degassing.


Type Vol Qty Cat.No.

Filtration plate

PP frits 20µm

Filtration plate

PTFE membrane 0.45µm

Filtration plate packed with

earth diatomaceous 100mg

Protein precipitation plates

and collection

Collection plate, round bottom

96 well cap mats

2mL

1mL

2mL

1mL

2mL

1mL

2mL

1mL

2mL

1mL

—

10

10

10

10

10

10

10

10

10

10

10

FTPP2002-01

FTPP2001-01

FTPF4502-01

FTPF4501-01

FTFT1002-01

FTFT1001-01

PPPC1002-01

PPPC1001-01

CLCM0002-01

CLCM0001-01

CLCM9600-0

96 Well Filtration Plates and Collection Plates

Accessories/S

upplie

s

165

Chemical Mixed Cellulose Acetate/nitrate Regenerate Cellulose Nylon PTEE

1-hexanol

Acetic acid,10%&25%

Acetone

Acetonitrile

Aliphatic hydrocarbon

Ammonia,1M

Aromatic hydrocarbon

Benzene

Boric acid

CTC

Carboxylic acid

Chloroacetic acid

Chloroform

Cyclohexane

Cyclohexanol

Diethyl ether

Dimethyl formamide

DMSO

Alcohol 98%

Acetic ether

Vinyl chloride

Ethylene glycol

Formic acid, 25%

N-hexane

Hydrochloric acid,25%

Isopropyl alcohol

Methanol

Nitrous acid,25%

Pentane

Phosphoric acid,25%

Phosphoric acid,45%

KOH,1M

Water solution of inorganic salt

NaOH,1M

Acetylene tetrachloride

THF

Toluene

TCA,10%

Trichloro ethane

Trichloroethylene

Dimethyl benzene

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

0

N/A

0

N/A

0

0

0

0

0

0

0

Chemical Resistance Guide for Membranes

The mark represent: “ ” = chemical resistance; “0” = limited chemical resisitant; “ ” = no chemical resistance ; N/A= no data.

Best Value





AgelaTechnologies


Accessories/S

upplie

s

166

2 mL 12 x 32 mm Screw Neck Vials and Caps

Vials and Caps

Vials

Agela Technologies Inc offers a number of high quality vials, caps, seals and crimping tools. Most of them are manufactured in Europe and

meet the high standard of the chromatographic applications. The difference of our offering from many other brand name venders is the cost to

you. We offer lower price for the same products as many brand name’ s due to our lean cost structure and we provide personal assist to you to

select right products

Description Thread Color Cat. No.(100/pk) Cat. No.(1000/pk)

Std

Std

Std

Std

short

short

short

Wide mouth 10/425

Wide mouth 10/425

Wide mouth 10/425

Clear

Amber

Clear

Amber

Clear

Clear

Amber

Clear

Clear

Amber

AV1000-0

AV1010-0

AV1001-0

AV1011-0

AV1100-0

AV1101-0

AV1111-0

AV1200-0

AV1201-0

AV1211-0

AV1000-1

AV1010-1

AV1001-1

AV1011-1

AV1100-1

AV1101-1

AV1111-1

AV1200-1

AV1201-1

AV1211-1

Screw neck vials

Screw neck vials

Screw neck vials, graduated


Screw neck vials



Screw neck vials



Description Thread Cat. No.(100/pk) Cat. No.(1000/pk)

Caps and Septa

Std

short

Wide mouth (10/425)

AV2000-0

AV2100-0

AV3100-0

AV2000-1

AV2100-1

AV3100-1

Screw cap

Screw cap

Screw cap (wide mouth)

2 mL 12 x 32 mm Crimp Neck Vials and Caps

Vials

Description Thread Color Cat. No.(100/pk) Cat. No.(1000/pk)

Description Thread Cat. No.(100/pk) Cat. No.(1000/pk)

Caps and Septa

Narrow mouth

Narrow mouth

Wide mouth

Narrow mouth

Narrow mouth

Wide mouth

Wide mouth

Clear

Amber

Clear

Clear

Amber

Clear

Amber

AV5000-0

AV5010-0

AV5001-0

AV5100-0

AV5101-0

AV5111-0

AV5200-0

AV5000-1

AV5010-1

AV5001-1

AV5100-1

AV5101-1

AV5111-1

AV5200-1

Crimp neck vials

Crimp neck vials

Crimp neck vials

Crimp neck vials, graduated



Crimp neck vials

Narrow mouth

Wide mouth

AV6000-0

AV6100-0

AV6000-1

AV6100-1

Crimp cap

Crimp cap

Plastic Vials (micro-vials for LC-MS autosamplers)

Description Material Vol. Cat. No.

PP

TPX

PP

Silicone/PTFE

300µL

300µL

—

—

AV0003-P

AV0003-T

AV0003-C

AV0003-S

Snap ring micro-vial32×11.6mm

Snap ring micro-vial32×11.6mm

Snap Cap

Septa

Documents

Agela