Bonna-Agela USA Bonna-Agela China Bonna-Agela India · Anylab Co.,LTD Tel: 02-2027-2628 Email: [email protected] South Korea Purichem Corporation Tel: +82-31-341-5507 Email: [email protected]

MAS-QuEChERS

Bonna-Agela

ABN: ZL-01105E

For Research Use Only. Not for use in diagnostic procedures.

All rights reserved.Bonna-Agela Technologies 2016.5 Rev.1 Official Website

Distributors:

ChileGenesys AnaliticaTel: (562) 2094704 Email: [email protected]

BrasilCMS Equipments for LabortoriesTel: 55-19-3882-2514Email: [email protected]

USAVWR International, LLCTel: (610) 386-1700Email: [email protected]

USAThermo Fisher Scientific Inc.Tel: 781.622.1000 / 800.678.5599 Email: [email protected]

SpainIRIDA IBERICA S.LTel: 34911130824 Email: [email protected]

Bonna-Agela USA2038A Telegraph Rd.Wilmington, DE 19808, USATel: (302) 48 8798Fax: (302) 636 9339Email: [email protected]

Bonna-Agela China179 South Street, Teda West Zone, Tianjin 300462, ChinaTel: +86 (22) 25321032/7023Fax: +86 (22) 25321033

Bonna-Agela India#212, 2nd Floor, Sector-63, Noida, Uttar PradeshTel: 91120-4225466/67Fax: 91120-4225465

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MAS-QuEChERS

BONNA-AGELA TECHNOLOGIES1

Quick, Easy, Cheap, Effective, Rugged and Safe

—— QuEChERS The QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) method was first introduced by USFDA and scientists in 2003 and it becomes increasingly popular in the area of multi-residue pesticide analysis in food and agricultural products.

www.bonnaagela.com 2

MAS-Q (QuEChERS)

MAS-Q (Multi-mechanism Adsorption SPE-QuEChERS) is an application of modified QuEChERS method which is suitable for most of the pesticides residue analysis, drug or antibiotic residue analysis in vegetable, fruit, grain, and animal issues.


Standard Operating Procedure

Transfer the supernatant toa MAS-Q tube

Shake Centrifuge and use thesupernatant as detect sample

Sample+Organic+Solvent+Salt Shake Centrifuge

Features ▲ Single extraction method could be applied to different kinds of sample matrix;▲ High recovery rate for multiple pesticides residue analysis;▲ Universal Clean-up kits are optimized for different application requirement; ▲ The method is simple, fast, reproducible and labor-saving.

● Step 1: ExtractionWeight comminuted sample (10 g or 15 g) into a 50mL centrifuge tube, and then open the extraction kit package, add the salt after adding your solvent to the sample.Extraction salts is helpful to extract the pesticides into the organic layer.Four glass homogeneous balls are suggested to add in to break the MgSO4 caking caused by sample rich in water.

● Step 2: CleanupTake proper supernatant from step one, and add it into a selected clean up tube which is suited to the food matrix also the method you are following.2 mL or 15 mL centrifuge tube containing different kind of SPE sorbent and MgSO4 is available for different sample volume. The sorbent will adsorb the interfering matrix, and the MgSO4 helps remove excess water.


MAS-QuEChERSUniversal Clean-up kits are optimized for different application requirement:▲ Cleanert® Pesticarb removes the pigments, including chlorophyll and carotenoids.▲ Cleanert® PSA removes organic acid.▲ Cleanert® C18 removes non-polar interferences, such as fats.▲ Anhydrous MgSO4 removes the excess water.

• The clean up kits are available in 2 mL, 15 mL for different sample volume requirement, and extraction kits are packed in anhydrous sealed packets; • Superior centrifuge tubes and glass homogeneous ball provide you a simple and convenient experience.

AOAC 2007.01

AOAC 2007.01-AOAC Method6 g MgSO4 (anhydrous) ;1.5 g NaAcP/N: MS-MG5052

General fruitand vegetable

2 mL Centrifuge tube for 1 mL sample50 mg PSA, 150 mg MgSO4, P/N: MS-PA0250

15 mL Centrifuge tube for 8 mL sample400 mg PSA, 1200 mg MgSO4, P/N: MS-PA1012

Fruits andvegetableswith fatsand waxes

2 mL Centrifuge tube for 1 mL sample50 mg PSA, 50 mg C18, 150 mg MgSO4, P/N: MS-9PA0203

15 mL Centrifuge tube for 8 mL sample400 mg PSA, 400 mg C18, 1200mg MgSO4, P/N: MS-9PA1011

Pigmentedfruits andvegetables

2 mL Centrifuge tube for 1 mL sample50 mg PSA, 50 mg PC, 150 mg MgSO4, P/N: MS-PP0250

15 mLCentrifuge tube for 8 mL sample400 mg PSA, 400 mg PC, 1200 mg MgSO4, P/N:MS-PP1550

EN 15662

EN 15662 -Europeanand Mini-MultiresidueMethod 4 g MgSO4

, 1 g NaCl, 1 g NaCitrate, 0.5 g disodium citratesesquihydrateP/N: MS-NMS5050

Original UnbufferedMethod 6 g MgSO4 (anhydrous);1.5 g NaClP/N: MS-MG50514 g MgSO4 (anhydrous);1 g NaClP/N: MS-MG5055

General fruitand vegetable

2 mLCentrifuge tube for 1 mL sample25 mg PSA, 150 mg MgSO4(anhydrous), P/N: MS-PA0251

15 mL Centrifuge tube for 6 mL sample150 mg PSA, 900 mg MgSO4(anhydrous), P/N:MS-PA1011

Fruits andvegetableswith fatsand waxes

2 mL Centrifuge tube for 1 mL sample25 mg PSA, 25 mg C18, 150 mg MgSO4(anhydrous)P/N: MS-9PA0204

15 mL Centrifuge tube for 6mL sample150 mg PSA, 150 mg C18, 900 mg MgSO4(anhydrous)P/N: MS-9PA1210

Pigmentedfruits andvegetables

2 mL Centrifuge tube for 1 mL sample25 mg PSA, 2.5 mg PC, 150 mg MgSO4(anhydrous)P/N: MS-PP0251

15 mL Centrifuge tube for 6 mL sample150 mg PSA, 15 mg PC, 900 mg MgSO4(anhydrous)P/N: MS-PP1511

Extraction Clean up

Production Selection Guide


Centrifuge > 1500 RPM 1 min Centrifuge > 3000 RPM/min

Centrifuge > 1500 RPM 1 minCentrifuge > 3000 U/min

Workflow--- Pesticide Residue Analysis in the Fruits and Vegetables

AOAC 2007.01 EN 15662

Add 6 g Anhydrous MgSO4 and 1.5 g Anyhydrous NaAc, shake vigorously 1 min.

Add 4 g Anhydrous MgSO4, 1 g Anhydrous NaAc, 1 g Sodium Citrate, 0.5 g disodiumcitrate sesquihydrate, shake vigorously.

Transfer supernatant to clean tube with Anhydrous MgSO4, PSA, C18 and GCB, shake.

Transfer supernatant to clean tube with 150 mg Anhydrous MgSO4, 25 mg PSA, 2.5 mg or 7.5 mg GCB (remove pigments, optional) , shake.

GC/MS analysis- dilute the extract with methylbenzene;LC/MS/MS analysis- dilute the extract with 6.7 mM formic acid.

GC/MS or LC/MS/MS analysis- dilute the extract with 5% HAc ( Acetic Acid) in ACH.

15 g homogenize sample + 15 mL 1% HAc ( Acetic Acid) in ACH in 50 mL tube, add internal standard and shake.

10 g homogenize sample + 10 mL MeCH in 50 mL tube, add internal standard and shake.


Sorbent Packet

Glass Homogenizer

MAS-Q Ordering Information

Package Part No.

Glass Homogenizer50/PK HG01

500/PK HG500

Extraction Kit (Without of centrifuge tube)50/PK

Extraction kit with 50 mL centrifuge tube50/PK

Extraction kit with 50mL centrifuge tube and glass homogenizer balls 50/PK

Standard Method

6 g MgSO4 (Anhydrous)1.5 g NaAc(Anhydrous) MS-MG5052-1 MS-MG5052 MS-MG5052-H Buffered

AOAC 2007.01 method

4 g MgSO4 (Anhydrous)1 g NaAc(Anhydrous) MS-MG5057-1 MS-MG5057 MS-MG5057-H Buffered

AOAC 2007.01 method

1 g NaCl 4 g MgSO4 (Anhydrous)0.5 g Disodium citrate sesquihydrate1 g NaCitrate

MS-NMS5050-1 MS-NMS5050 MS-NMS5050-H Buffered EN 15662 method, 10 g samples

4 g MgSO4(Anhydrous)1 g NaCl MS-MG5055-1 MS-MG5055 MS-MG5055-H Original method, 15 g

samples

6 g MgSO4(Anhydrous)1.5 g NaCl MS-MG5051-1 MS-MG5051 MS-MG5051-H Original method, 15 g

samples

6 g MgSO4(Anhydrous)1.5 g NaAc.3H2O

MS-MG5050-1 MS-MG5050 MS-MG5050-H BufferedAOAC 2007.01 method


MAS-QuEChERS Cleanup Kits

Cleanup Kits, Fruits and Vegetables Samples

General fruits and vegetables, such as apple, cabbage, cucumber

50 mg PSA，150 mg MgSO4(Anhydrous) AOAC 2007.01 2 mL centrifuge

tube 100/PK MS-PA0250

400 mg PSA，1.2 g MgSO4(Anhydrous) AOAC 2007.01 15 mL centrifuge


25 mg PSA，150 mg MgSO4(Anhydrous) EN 15662 2 mL centrifuge


150 mg PSA，900 mg MgSO4(Anhydrous) EN 15662 15 mL centrifuge


Fruits and vegetables with fats and protein, such as potato, corn, avocado

50 mg C18, 50 mg PSA，150 mg MgSO4(Anhydrous)

AOAC 2007.01 2 mL centrifuge tube 100/PK MS-9PA0203


AOAC 2007.01 15 mL centrifuge tube 50/PK MS-9PA1011


EN 15662 2 mL centrifuge tube 100/PK MS-9PA0204


EN 15662 15 mL centrifuge tube 50/PK MS-9PA1210

100 mg PSA，100 mg C18，300 mg MgSO4(Anhydrous)

NYT 1380-2007 15 mL centrifuge tube 50/PK MS-9PA1010

Fruits and vegetables rich in pigment, such as carrot, spinach, lettuce

25 mg PSA, 2.5 mg PestiCarb, 150 mg MgSO4(Anhydrous)

EN 15662 2 mL centrifuge tube 100/PK MS-PP0251

150 mg PSA, 15 mg PestiCarb, 900 mg MgSO4(Anhydrous)


25 mg PSA, 7.5 mg PestiCarb, 150 mg MgSO4(Anhydrous)


50 mg PSA，50 mg PC，150 mg MgSO4(Anhydrous)

AOAC 2007.01 2 mL centrifuge tube 100/PK MS-PP0250

150 mg PSA，50 mg PC，900 g MgSO4(Anhydrous)


400 mg PSA，400 mg PC，1200 mg MgSO4(Anhydrous)

AOAC 2007.01 15 mL centrifuge tube 50/PK MS-PP1550

Part No.Contents Method Tube SizeSample Type


Drugs analysis in plasma

Basic drug50 mg PAX 2 mL centrifuge

tube 100/PK MS-AX0250

200mg PAX 15 mL centrifuge tube 50/PK MS-AX1020

Acidic drug50 mg PCX 2 mL centrifuge

tube 100/PK MS-CX0250

200 mg PCX 15 mL centrifuge tube 50/PK MS-CX1020

Neutral drug50 mg PEP 2 mL centrifuge

tube 100/PK MS-PE0250

200 mg PEP 15 mL centrifuge tube 50/PK MS-PE1020

Highly protein content dairy sample, milk

Melamine in complex matrix (fodder, chocolate, flour, fish, meat)

Blend of ion exchange and reversed phase materials

50 mL centrifuge tube 50/PK MS-SPC5001

Melamine in dairy sample 50 mL centrifuge tube 50/PK MS-SPM5001

Dicyandiamide in dairy sample

15 mL离心管50/PK MS-SQA02

Part No.

Part No.

Contents

Contents

Tube Size

Tube Size

Sample Type

Sample Type

Complicated Sample matrix rich in pigment and fatty

50 mg PSA, 50 mg C1850 mg PC150 mg MgSO4(Anhydrous)

AOAC 2007.01 2 mL centrifuge tube 100/PK MS-9PP0250

400 mg PSA, 400 mg C18400 mg PC1200 mg MgSO4(Anhydrous)

AOAC 2007.01 15 mL centrifuge tube 50/PK MS-9PP0253

150 mg PSA, 150 mg PC150 mg C18900 mg MgSO4(Anhydrous)

15 mL centrifuge tube 50/PK MS-9PP0252

Empty centrifuge tube

50 mL 50 mL centrifuge tube 50/PK LXG0050

15 mL 15 mL centrifuge tube 50/PK LXG0015

2 mL 2 mL centrifuge tube 100/PK LXG0002-L

Cleanup Kits for dairy sample

Cleanup Kit for biological sample

Part No.Contents Method Tube SizeSample Type


Analysis of Mult-pesticide Residues in Apple using MAS-QuEChERS Kits (AOAC 2007.01)

● Weight 5 g comminuted apple sample into a 50 mL centrifuge tube● Add 15 mL MeOH with 0.1% HAc into the sample● Open the MAS-QuEChERS Extraction Kits (P/N: MS-MG5050) and transfer the material into the sample also four glass homogenizers● Shake by hand for 1 min

● Take 10 mL supernatant into a 15 mL MAS-QuEChERS Cleanup Tube (P/N: MS-9PA1011)● Shake by hand for 1 min

● Take 5 mL supernatant from step two and concentrate it by N2 evaporator at 40℃ ,reconstitute the residue with 1mL acetone and then for GC/MS analysis● Take another 5 mL supernatant and concentrate it by N2 evaporator at 40℃ , reconstitute the residue with 1 mL ACN and then for HPLC analysis.

Analysis

Extraction

Cleanup

Centrifuge 5 min, 8000 r/min


Ordering InformationProduct Name Specification Package Part No.

MAS-QuEChERS Extraction Kits

6 g MgSO4(Anhydrous), 1.5 g NaAc.3H2O with50 mL centrifuge tube 50/PK MS-MG5050

MAS-QuEChERS Cleanup Tube

400 mg C18, 400 mg PSA, 1200 mg MgSO4(Anhydrous), with 15 mL centrifuge tube

50/PK MS-9PA1011

1.5 mL vials Short thread, 32×11.6 mm 100/pk 1109-0519

Caps and SeptaScrew neck cap, short 9 mm, center hole;red silicone/ white PTFE septa 45° Shore A; 1.0 mm

100/pk 0915-1819

Nylon Syringe Filters 13 mm, 0.22 μm 200/PK AS021320

Disposable Syringe 2 mL, no needle 100/PK LZSQ-2ML


● Weight 10 g comminuted sample in 50 mL centrifuge tube● Add 10 mL ACN into the sample firstly open the MAS-QuEChERS Extraction Kits (P/N: MS- NMS5050) and transfer the material into the sample also four glass homogenizers● Shake by hand for 1 min

● Take out 1 mL supernatant and add it into MAS-QuEChERS cleanup tube (P/N: MS-PA0215)● Shake by hand for 1 min

● Take the supernatant then for LC-MS/MS analysis



Ordering InformationProduct Name Specification Package Part No.

MAS-QuEChERS Extraction Kits1 g NaCl, 4 g MgSO4 (Anhydrous),0.5 g Disodium citrate sesquihydrate,1 g NaCitrate, with 50 mL centrifuge tube

50/PK MS-NMS5050

MAS-QuEChERS Cleanup Kits 25 mg PSA, 150 mg MgSO4(Anhydrous), 2 mL centrifuge tube 100/PK MS-PA0251

Glass homogenizers Diameter: 10 mm 50/PK HG01

1.5 mL vials Short thread, 32×11.6 mm 100/PK 1109-0519

Caps and SeptaScrew neck cap, short 9 mm, center hole;red silicone/ white PTFE septa 45° Shore A; 1.0 mm

100/PK 0915-1819

Nylon Syringe Filters 13 mm, 0.22 μm 200/PK AS021320

Disposable Syringe 2 mL, no needle 100/PK LZSQ-2ML

Analysis

Extraction

Cleanup

Analysis of Multi-pesticide Residues in Cabbage using MAS-QuEChERS Kits Using LC-MS/MS (EN 15562)


Product FormatsOptimized sorbent was packed into a syringe filter format cartridge and make it could be operated with a simple two-Step operation, conditioning, loading sample and collect the eluent.This kind of design make it could be easily compatible with an automated workstation for high throughput requirement.

Application NoteDetection of 112 kinds of pesticide residue in black tea leaves sample using LC-MS/MS method

Cleanert® NANO —— A New Material of Carbon Nanotube

Cleanert® NANO was developed base on carbon nanotube material. After Functionalized process, the nano material has better affinity to remove colorants and fatty acids. And the surface deactivation process could control the over-adsorption within limited area, and ensure the recovery of pesticide with a benzene ring structure. The material which has a layer stacked structure could significantly increase the specific surface area and also the loading capacity.

These characteristics allow much less use of the material and improve specificity and selectivity. The amount of material used per sample is about only 1/10 to 1/5 when comparing to traditional PSA, C18 or GCB SPE material.It could be widely used in the analysis of multiple-pesticide residues in fruit, vegetable and other agro-products. Small amount of the nano material about 10-15mg is enough for most of the sample matrix, and small sorbent bed make it could be packed as a filter format cartridge and show more benefit for fast analysis and small amount of sample analysis requirement.

10 g Sample + 10 mL water and stand for 1h

Shake by hand for 1 mins and then centrifuge at 8000 r/min

Add 15mL ACN firstly into the sample, and then add the extraction salts 4 g MgSO4 +1 g NaCl (P/N: MS-MG5055) into the sample also four glass homogenizer

Take 1 mL Supernatant, and pass the sample through the NANO cartridge containing 150 mg MgSO4 + 10 mg MWCNTs (PN: IC-NN1510-C)

Repeat the last step three times, and collect all the solution, filtered by Nylon filter and then for LC-MS/MS analysis

Sample Preparation Procedure

Structure of the Cleanert® Nano

Syringe Filter Format


Figure 1. LC-MS/MS chromatogram of tea leaves extract. (A) 114 kinds of pesticides standard solution; (B) tea leaves extract spiked with 0.02 μg/mL 114 kinds of pesticides standard solution.

Cleanup performance comparison for leek sample: cleanert nano(left), PSA (middle) and before cleanup (right)

Ordering Information

A

B

The recoveries for 114 kinds of pesticides are from 60 to 110%, and the RSD is lower than 10%.

Product Name Specification Package Part No.

Cleanert® NANONano tube for Complex Samples

10 mg Nano, 150 mg MgSO4 50/PK IC-NN1010-V

MAS-QuEChERS Cleanup Kits 15 mg Nano, 150 mg MgSO4 50/PK IC-NN1510-C

Sample Preparation ProductsBonna-Agela

Official Website


Official Website


Official Website

Bonna-Agela USA2038A Telegraph Rd.Wilmington, DE 19808, USATel: (302) 48 8798Fax: (302) 636 9339Email: [email protected]

Bonna-Agela China179 South Street, Teda West Zone,Tianjin 300462, ChinaTel: +86 (22) 25321032/7023Fax: +86 (22) 25321033

Bonna-Agela India#212, 2nd Floor, Sector-63, Noida,Uttar PradeshTel: 91120-4225466/67Fax: 91120-4225465

UK & IrelandKinesis Ltd

T: +44 (0)1480 212122E: [email protected] W: kinesis.co.uk

Distributed by

GermanyKinesis GmbH

T: +49 (0)2173 89 05-0 E: [email protected] W: kinesisgmbh.de

AustraliaKinesis Australia Pty Ltd

T: +61 (0)7 3829 3996E: [email protected] W: kinesis-australia.com.au

ItalyABreg S.r.l

T: +39 0131 389513E: [email protected] W: abreg.com

Documents

Bonna-Agela USA Bonna-Agela China Bonna-Agela India · Anylab Co.,LTD Tel: 02-2027-2628 Email: [email protected] South Korea Purichem Corporation Tel: +82-31-341-5507 Email: [email protected]