E-beam lithography gold nanoparticle arrays for nano-biosensor development

Maher Z. AhmedPhD research project

Health care Environment safety

Why biosensor? High performance bio and chemical sensor

What is a biosensor?

Enhance selectivity

very high selective chemistry. DNA strains or specialist enzymeThe advanced new sensors ResearchesEnhance sensitivity

improving coupling between the chemical detection and transducer

improving transducer

Physics of nanoparticles offers a promising future in that field. Top-Down approachBottom-up approach

Enhance sensitivity Signal Acquisition

The advanced new sensors Researchesadvanced technology in optical spectroscopy instrumentations

Aucatcher or probe oligonucleotides biotin-coupling group Glass substrate complementary oligonucleotide targetLightMy Target biosensor?

HealthyPatientHaes AJ, Chang L, Klein WL, Van Duyne RP. 2005. Detection of a biomarker for Alzheimers disease from synthetic and clinical samples using a nanoscale optical biosensor. J. Am. Chem. Soc. 127:226471

Songbo Xu PhD. thesis Surface Plasmon Spectroscopy (SPS) to monitor the bio chemistry reaction

Songbo Xu PhD. thesis W. Knoll et al. : Colloids and Surfaces A: Physicochem. Eng. Aspects 161 (2000)

1- e-beam lithography system is a LEO 1530 field emission scanning electron microscope (FE-SEM)

2- Solitec 5110 Spinner/Developer

3-Custom e-beam Deposition (Hoser)

4- Zeiss Axioskop2 Mat MicroscopeE-beam lithography Nano gold particle arraysFabrication and characterization (NanoFab)

E-beam lithography Nano gold particle arrays

e_Glass substrataPMMAE-beam lithographyDevelopingChromiumChromiumGoldLift Off

Signal AcquisitionOld sample cuvette Needs for New design Minimum Volume target

Who Wants to Be a Millionaire?Future Applications for our optimum system

Cooperation with Dr. Bulent Mutus, Biochemistry Research Group, U. Windsor AuAUtype 2 diabetes indications enzymeCutting the double S-S bondType 2, where the body makes insulin but cannot use it properly

Chart1

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