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Additional File 2: Linear range of ERCC detection (A) microarray (B) RT-qPCR platforms: ERCC-42. A. B. Additional File 2: Linear range of ERCC detection (A) microarray (B) RT-qPCR platforms: ERCC-81. A. B. - PowerPoint PPT Presentation
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Additional File 2: Linear range of ERCC detection (A) microarray (B) RT-qPCR platforms: ERCC-42
y = 1.0047x - 9.5772
R2 = 0.9675
-8
-6
-4
-2
0
2
4
6
8
10
12
0 5 10 15 20 25
Log2[RNA copies/ng total RNA]
Lo
g2(
No
rma
lise
d s
ign
al)
y = -1.0205x + 42.617
R2 = 0.9988
20
22
24
26
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30
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34
36
38
40
0 5 10 15 20 25
LogPCR Efficiency[RNA copies/ng total RNA]
Ct
A
B
Additional File 2: Linear range of ERCC detection (A) microarray (B) RT-qPCR platforms: ERCC-81
y = 1.0389x - 10.155
R2 = 0.9671
-10
-8
-6
-4
-2
0
2
4
6
8
10
0 5 10 15 20 25
Log2[RNA copies/ng total RNA]
Lo
g2(N
orm
alis
ed s
ign
al)
y = -0.9946x + 40.515
R2 = 0.9992
18
20
22
24
26
28
30
32
34
36
38
40
42
0 5 10 15 20 25
LogPCR Efficiency[RNA copies/ng total RNA]
Ct
A
B
Additional File 2: Linear range of ERCC detection (A) microarray (B) RT-qPCR platforms: ERCC-84
y = 0.9772x - 5.8678
R2 = 0.9639
-6
-4
-2
0
2
4
6
8
10
12
14
16
0 5 10 15 20 25
Log2[RNA copies/ng total RNA]
Lo
g2(N
orm
alis
ed s
ign
al)
y = -0.9693x + 41.49
R2 = 0.9986
20
22
24
26
28
30
32
34
36
38
40
0 5 10 15 20 25
LogPCR Efficiency[RNA copies/ng total RNA]
Ct
A
B
Additional File 2: Linear range of ERCC detection (A) microarray (B) RT-qPCR platforms: ERCC-95
y = 0.957x - 8.8564
R2 = 0.9712
-8
-6
-4
-2
0
2
4
6
8
10
12
0 5 10 15 20 25
Log2[RNA copies/ng total RNA]
Lo
g2(N
orm
alis
ed s
ign
al)
y = -1.0022x + 44.939
R2 = 0.9992
22
24
26
28
30
32
34
36
38
40
42
0 5 10 15 20 25
LogPCR Efficiency[RNA copies/ng total RNA]
Ct
A
B
Additional File 2: Linear range of ERCC detection (A) microarray (B) RT-qPCR platforms: ERCC-99
y = 1.0095x - 6.6213
R2 = 0.9626
-8
-6
-4
-2
0
2
4
6
8
10
12
14
0 5 10 15 20 25
Log2[RNA copies/ng total RNA]
Lo
g2(N
orm
alis
ed s
ign
al)
y = -1.0052x + 41.88
R2 = 0.9984
20
22
24
26
28
30
32
34
36
38
40
0 5 10 15 20 25
LogPCR Efficiency[RNA copies/ng total RNA]
Ct
A
B
Additional File 2: Linear range of ERCC detection (A) microarray (B) RT-qPCR platforms: ERCC-113
y = 1.0248x - 6.6933
R2 = 0.9993
-6
-4
-2
0
2
4
6
8
10
12
14
0 5 10 15 20 25
Log2[RNA copies/ng total RNA]
Lo
g2(N
orm
alis
ed s
ign
al)
y = -1.02x + 42.406
R2 = 0.9974
20
22
24
26
28
30
32
34
36
38
40
0 5 10 15 20 25
LogPCR Efficiency[RNA copies/ng total RNA]
Ct
A
B
Additional File 2: Linear range of ERCC detection (A) microarray (B) RT-qPCR platforms: ERCC-171
y = 1.0696x - 10.718
R2 = 0.9993
-10
-8
-6
-4
-2
0
2
4
6
8
10
12
0 5 10 15 20 25
Log2[RNA copies/ng total RNA]
Lo
g2(N
orm
alis
ed s
ign
al)
y = -0.9714x + 44.299
R2 = 0.9981
22
24
26
28
30
32
34
36
38
40
42
44
0 5 10 15 20 25
LogPCR Efficiency[RNA copies/ng total RNA]
Ct
A
B
