EFEK DEKOKTA PELEPAH DAUN NIPAH (Nypa fruticans Wurmb.) TERHADAP
KADAR TUMOR NECROSIS FACTOR ALPHA (TNF-) HEPAR TIKUS MODEL DIABETES
MELLITUS TIPE-2M. Karunia Fadillah1, Helmin Elyani2, Arif Yahya21
Mahasiswa Fakultas Kedokteran Universitas Islam Malang
2 Staf Pengajar Fakultas Kedokteran Universitas Islam Malang
Email: [email protected] :
Komplikasi DMT-2 berkaitan dengan penurunan fungsi hepar akibat
kematian sel hepar. Hiperglikemia kronis mampu mengaktivasi
mediator inflamasi seperti IL-6, IL-18, IL-1 dan TNF- pada hepar.
Hal tersebut dapat secara langsung menginduksi jalur kematian sel
berupa apoptosis dan meningkatkan formasi dari advanced glycated
end products (AGEs), autooksidasi glukosa, dan oksidasi fosforilasi
yang dapat meningkatkan reactive oxygen species (ROS) sehingga
menimbulkan kondisi stres oksidatif. Paparan ROS dapat menimbulkan
peroksidasi lipid dan mengaktivasi sel kupffer sehingga
meningkatkan pelepasan mediator inflamasi, diantaranya TNF-.
Pelepah daun nipah (Nypa fruticans Wurmb.) memiliki potensi sebagai
antidiabetik, antioksidan, dan antiinflamasi. Tujuan penelitian ini
adalah untuk membuktikan efek dekokta pelepah daun nipah terhadap
kadar TNF-( hepar pada tikus model DMT-2.
Metode: Penelitian in vivo ini menggunakan desain control group
post test only menggunakan tikus Wistar jantan. Tikus model DMT-2
diinduksi menggunakan High Fructose Diet (HFD) dan Streptozotocin
(STZ) single dose 35 mg/kgBB. Pemberian dekokta pelepah daun nipah
dosis 200, 400 dan 800 mg/kgBB diberikan personde selama 30 hari.
Pengukuran kadar TNF-( hepar tikus menggunakan metode ELISA
indirect. Data dianalisa dengan uji one way ANOVA, kemudian
dilanjutkan dengan LSD. Hasil dikatakan bermakna bila p0.05.Hasil:
Pemberian dekokta pelepah daun nipah dosis 200 mg/kgBB, 400
mg/kgBB, 800 mg/kgBB mampu menurunkan kadar TNF-( hepar tikus
berturut-turut sekitar 10%, 29%, dan 32% dibandingkan dengan
kelompok kontrol positif (p0.05).Kesimpulan: Dekokta pelepah daun
nipah dosis 200 mg/kgBB, 400 mg/kgBB, 800 mg/kgBB mampu menurunkan
kadar TNF-( hepar pada tikus model DMT-2.Kata Kunci: Nypa fruticans
Wurmb., Diabetes Melitus Tipe-2 (DMT-2), kadar TNF-( hepar.DECOCTED
MIDRIB OF NIPAH LEAF (Nypa fruticans Wurmb.) EFFECT TO THE HEPATIC
TUMOR NECROSIS FACTOR ALPHA (TNF-) ON TYPE 2 DIABETES MELLITUS RAT
MODELM. Karunia Fadillah1, Helmin Elyani2, Arif Yahya2
1 Undergraduate Student of Medical Faculty Islamic University of
Malang2 Lecturer at Medical Faculty Islamic University of
MalangEmail: [email protected]: DMT-2
complications usually connected to reduced hepatic function due to
cell death mechanism. Chronic hyperglycemia can induce hepatic
mediators of inflammation such as IL-6, IL18, IL-1 and TNF-. Thus,
it will directly induce cell death pathway mechanism like apoptosis
and also increase formation of advanced glycated end products
(AGEs), glucose auto oxidation and Oxidative phosphorylation that
can increase the amount of reactive oxygen species (ROS) which
resulted in oxidative stress. Exposure with ROS can induce lipid
peroxidation and activation of kupffer cell that will also increase
the amount of released inflammatory mediators such as TNF-. The
midrib part of nipah leaf (Nypa fruticans Wurmb.) was known to be
potentially having anti-diabetic, anti-oxidant and
anti-inflammation effect. This research aims to prove the decocted
nipah leaf effect to the hepatic TNF- level of DMT-2 rat
models.
Methods: This in vivo research was using control group post-test
only design on male wistar rats. DMT-2 rat model were induced with
High Fructose Diet (HFD) and 35 mg/Kgbw of Streptozotocin (STZ)
single dose. Supplementation of decocted nipah leaf with the dose
of 200, 400 and 800 mg/kgbw was given orally for 30 days. TNF-(
level were measured using indirect ELISA. The data were analized
with one way ANOVA test continued with LSD. The result will be
significant if p0.05Result: Oral administration of Nipah leaf with
the dose of 200 mg/kgbw, 400 mg/kgbw, 800 mg/kgbw was able to
reduce hepatic TNF-( level respectively by 10%, 29%, dan 32%
compared to control positive group (p0.05).Conclusion: Decocted
midrib part of nipah leaf with the dose of 200 mg/kgBB, 400
mg/kgBB, 800 mg/kgBB were able to reduce hepatic TNF-( level on
DMT-2 rat model.Keywords: Nypa fruticans Wurmb., Type-2 Diabetes
Melitus (DMT-2), hepatic TNF-( level.
