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A New Era in High Speed Analysis with Dynamic MRM and UHPLC

Tim Schlabach, Marketing

Anabel Fandino, Ph.D., R&D

PeteStone, Ph.D., Marketing

ASMS TrainingPage 2

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Agilent LC/MS, GC/MS, and ICP-MS Products

ASMS TrainingPage 3

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Agilent LC/MS, GC/MS, and ICP-MS Products

Agilent 1290 Infinity LC Attributes for MS - Infinitely Better for LC/MS

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1200bar @ 2mL/min for highest resolutionReduced Ion & Matrix Suppression

HT-Solution for up to 2,000 samples/dayComplete System Integration MassHunter

Less than 100 uL delay volumesFirst peaks can elute in < 6 seconds

High Pressures

LowDelay Vol.

High Throughput

Agilent 6000 Series LC/MS: Speed for UHPLC

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Fast MRMsfor UHPLC

40 MS Scans/sec10 MS/MS scans/sec

BestData

QualityFast Scans for UHPLC

Agilent 6460

Agilent 6530

1 ms dwell for MRM30 ms Pos/Neg Switching> 200 MRMs/ sec

1290 Infinity LC means fast peaks, < 3 seconds

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Fast MRMs with 6400 Series QQQ – metabolism polarity switching in 30 millisecs plus 6 transitions (2 ISTDs)

Avg W1/2 = 1.3 sec 35 points across RSD [%] = 1.8

Avg W1/2 = 0.71 sec 15 points across RSD [%] = 2.9

Avg W1/2 = 1.3 sec 35 points across RSD [%] = 2.7

Avg W1/2 = 0.52 sec 11 points across RSD [%] = 6.5

Verapamil, 0.75 min

Buspirone, 0.40 min

Dextromethorphan, 0.53 min

Diclofenac, 1.04 min

Peaks widths with UHPLC only 2 seconds or less > ten uniform measurements across peak REQUIRED

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W = 2.1 sec

Cycle time < 115 ms

Avg. W1/2 = 0.72 sec

Verapamil, peak RSD 5%

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Peak Capacities are very high with UHPLC 40 Extracted Ion Transitions in 1 minute

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Dynamic MRM for 6400 Series Triple Quads monitors ion transitions only when compounds elute

With DMRM the number of concurrent MRMs are fewer than with Time Segments

Cycle Time:Concurrent MRMsDwell Time:

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Agilent 1290 Infinity LC + 6460 Triple Quad

9 replicates analyzed at 200 pg levelMean area %RSD = 3.2

Analysis of 600 Pesticide DMRM Transitions in 15 min.

17 min.

1290/6230 Accurate Mass TOF Pesticide Screening250 pesticides, 50 pg on column

3 min.

1.5 min.

Most peaks 1-2 seconds wide!

The Need for Speed in UHPLC/MS/MS

Cycle Times (MRM): <150 milliseconds

Dwell Times (MRM or DMRM): <= 2 millliseconds

Polarity Switching (MRM): < 50 milliseconds

Spectral scans: at 5 Hz or faster

PPM Mass Accuracy: at 5 Hz

20 K Resolution: at 5 Hz

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Agilent LC/MS can meet your most demandingapplication needs.

1290 Infinity LC and 6460 Triple Quad MS

1290 Infinity LC and 6530 Accurate Mass Q-TOF MS

The ultimate Qual solution

The ultimate Quant solution