A 3-week Biotech Unitfor Any Biology Class

Ellyn DaughertySMBCP, San Mateo CA

Educator/Authorwww.BiotechEd.comwww.SMBiotech.com

www.emcp.com/biotechwww.sargentwelch.com/biotech

Biotech in Biology3 weeks (15 hours)

> What is Biotechnology? Computer Search Collage

> Micropipeting Activity

> DNA Spooling Activity

> DNA Fingerprinting Using RFLPs Activity

> Recombinant DNA Technology

> Bacterial Transformation (GMO) Activity

> Bioethics of Engineering Organisms

> Careers in Biotechnology

Materials, Supplies, Funds Needed for Biotech in Biology Unit

Class set of:

•4 micropipets/tips each (P-1000, P-200, P-20)

•4 gel boxes, 2 power supplies

•DNA Spooling Activity Kit

•DNA Fingerprinting Activity Kit

•Bacteria Transformation Activity Kit

•Other supplies and materials (96-well plates, food coloring, 37°C incubation oven, water bath

= about $300 in kits/ class= about $3000 for school set of micropipets and gel apparatus

What Is Biotechnology?

Micropipeting Activity

Show me:

Pipet 135 µL

or 235 µL

into a tube

What Does DNA Look Like?

(Edvotek #S-10)

= one 50 min class period (30 min activity, 20 min discussion

$39 / 10 lab groups

DNA Spooling Activity

Do Onions Have DNA (or other fruits

or veggies) ?

(Edvotek #S-75)

= one 50 min class period (30 min activity, 20 min discussion

$49 / 5 lab groups… or use my Lab 4b Materials from SW List

DNA Fingerprinting Using RFLPs Activity

Ward’s DNA Detectives Lab Activity #109)

= four 50 min class periods

$115 / 8 lab groups

Edvotek’s

DNA Fingerprinting by Restriction Fragmentation Patterns #109)

= four 50 min class periods

$79 / 6 lab groups

Recombinant DNA

Technologyuse

http://www.dnalc.org/resources/animations/

Use the Restriction Digestion animation to discuss:

• Restriction enzymes

• DNA ligase

• Recombinant DNA (rDNA)

Use the Transformation animation to discuss:

• Insertion of rDNA vector

• Selection and Expression of newly insert genes

• Applications of rDNA technology

Bacterial Transformation (GMO) Activity

Ward’s Improved Bacterial Transformation Using GFP #36 V 6359

= four 50 min class periods

$85/ 8 lab groups

Edvotek’s Transformation with Green Fluorescent Protein

#223 and #969

= four 50 min class periods

$110/ 10 lab groups

Biotechnology Career Research e-Project

Bioethics of Engineered Organisms

Materials Lists

www.sargentwelch.com/biotech

Find your Sales Rep for materials/equipment/

support

Biotechnology: Science for the New Millennium

Ellyn Daughertyellyn@BiotechEd.com

•Text with Encore CD•Lab Manual•Instructor’s Guide •Course Planner (Lesson

Plans)•Instructor Resource Center

on web•Student Notebook, Test

Generator, etc. available

www.BiotechEd.com for tour through the entire curriculum, ancillaries, websites, and professional development opportunities