8 International Workshop on Antigen Processing & Presentation...proteins govern their sampling for HLA class I presentation 21. Gosia Garstka -The Netherlands Cancer InstituteThe specificity

8th International Workshop on Antigen

Processing & Presentation

10-13 June 2014 The Academy of Natural Sciences of Drexel University

Philadelphia, PA, US

Organizers: Laurence Eisenlohr – Thomas Jefferson University Lisa Denzin – Rutgers Child Health Institute of NJ

Tim Elliot - University of Southampton Paul Roche - NCI/National Institutes of Health

8th International Workshop on Antigen Processing & Presentation

Tuesday, June 10, 2014

Tuesday 2

5:00 Registration

7:00 Welcome – Laurence Eisenlohr, Thomas Jefferson University

7:10 KEYNOTE 1 Nilabh Shastri, University of California, Berkeley On the origins and anatomy of the peptide repertoire displayed by MHC class I molecules


Wednesday, June 11, 2014

Wednesday 3

SESSION 1: PROTEOLYSIS AND PROCESSING CO-CHAIRS: Jonathan Yewdell - NIAID/National Institutes of Health Jack Bennink - NIAID/National Institutes of Health

9:00 Organizational Comments and Chair’s Introduction

9:05 Michel Desjardins - University of Montreal - Selective capture of antigens derived from subcellular compartments by autophagy for MHC class I presentation

9:25 Robin Fårhaeus – INSERM - Pioneer translation products as the source of peptides for the MHC class I pathway

9:40 Margarita Del Val - Centro de Biología Molecular Severo Ochoa (CSIC-UAM) - N-ras couples antigen receptor signaling to Eomesodermin and to functional CD8+ T cell memory but not to effector differentiation

9:50 Sylvie Le Gall - The Ragon Institute of MGH, MIT and Harvard - Differential cross-presentation of HIV epitopes by dendritic cells and macrophages identifies variable intracellular degradation rates of HIV peptides

10:00 Frédéric Ebstein - Charité Universitätsmedizin Berlin - An antagonistic role for the structure and the activity of immunoproteasomes in the generation of a new spliced MHC class I melanoma tumour epitope formed through a condensation reaction

10:10 BREAK –light refreshments served

10:30 David Tscharke – The Australian National University - Towards a full viral MHC class I immunopeptidome: composition, kinetics and immunogenicity

10:45 Edward James – University of Southampton - Functionally distinct ERAP1 haplotype combinations distinguish individuals with Ankylosing Spondylitis

11:00 Irini Evnouchidou – INSERM - ERAP1-ERAP2 dimerization increases peptide trimming efficiency

11:15 Laura Santambrogio – Albert Einstein College of Medicine - Contribution of the Lymph Antigens to the MHC II self-peptidome

11:30 Scheherazade Sadegh-Nasseri – Johns Hopkins University School of Medicine - Multiple Paths to Immunodominance: dominant epitopes are captured by MHC II prior, or post antigen proteolysis, and are enriched by HLA-DM

11:45 Janice Blum – Indiana University School of Medicine - Altered Antigen Presentation and BCR Trafficking with Nutrient Deprivation

12:00 LUNCH - Academy Commons (1st Floor)



Wednesday 4

SESSION 2: ASSEMBLY AND LOADING CO-CHAIRS: William Hildebrand - University of Oklahoma Health Sciences Center Lawrence Stern - University of Massachusetts Medical School

1:00 Chair’s introduction

1:05 Anthony Purcell - Monash University - Redefining Antigen Binding specificity through small molecule-HLA interactions: Drug hypersensitivity and beyond!

1:25 Yuri Sykulev – Thomas Jefferson University - Nanolipoprotein discoidal bilayers as membrane mimetics of MHC clustering

1:40 Peter Jensen – University of Utah - Rate saturation in HLA-DM catalyzed peptide exchange

1:55 Jacques Thibodeau – University of Montreal - The human invariant chain isoform p35 promotes formation of nonameric complexes with MHC class II molecules and is needed for copII-mediated ER egress

2:05 Malini Raghavan – University of Michigan – Distinct assembly profiles of HLA-B molecules

2:15 Clemens Hermann – University of Cambridge - Tapasin-related protein restricts the MHC class I peptide repertoire by enhancing peptide exchange

2:30 BREAK – light refreshments served

3:00 David Margulies – NIAID/National Institutes of Health - Tapasin-related protein TAPBP-R interacts directly with peptide-free MHC-I/b2-microglobulin complexes and is released by high affinity peptides

3:15 Richard Birkinshaw – Monash University - T cell activation by transitory neo-antigens derived from distinct microbial pathways

3:35 Luc Teyton – The Scripps Research Institute - The identification of the endogenous ligands of Natural Killer T cells reveals the existence of mammalian alpha-linked glycosylceramides

3:55 Tim Elliott – University of Southampton - MHC class I function is determined by protein plasticity

4:15 Jörn Werner– University of Southampton - Structural plasticity and antigen selection by MHC 1



Wednesday 5

4:30 – 5:00 SPECIAL SESSION Timothy Gondre-Lewis - National Institutes of Health/NIAID Applying for a Grant at the NIH/NIAID 4:30 POSTER SESSION 1 – North American Hall

1. Annegret Bitzer - University of Konstanz - Antigen Fusion To Ubiquitin-like Modifier FAT10 Enhances Direct Presentation On MHC Class I

2. Julie Boucau – The Ragon Institute of MGH, MIT and Harvard - Effect of HIV infection on the antigen processing machinery of primary CD4 T cells

3. Emilie Duvallet – Université Paris Sud - Pioneer translation products in the direct and the cross-presented MHC class I pathways

4. Irini Evnouchidou - INSERM U1013 - ERAP1-ERAP2 dimerization increases peptide trimming efficiency

5. Asha Ganesan and Michael Miller – Thomas Jefferson University - Endogenously-processed influenza epitopes intersect with the classical MHCII presentation pathway via the GILT endosomal reductase

6. William Hildebrand - University of Oklahoma Health Science Center - Identification of Mtb Peptides Presented by the HLA-Ib Molecule HLA-E

7. Georgio Kourjian - The Ragon Institute of MGH, MIT and Harvard - Sequence-specific alterations of epitope production and presentation by HIV Protease Inhibitors

8. Sylvie Le Gall - The Ragon Institute of MGH, MIT and Harvard - Differential cross-presentation of HIV epitopes by dendritic cells and macrophages identifies variable intracellular degradation rates of HIV peptides

9. Zachary Maben - University of Massachusetts Medical School - Novel and specific small-molecule inhibitors of ERAP1

10. Sarah Mundt - University of Konstanz - Selective inhibition of the immunoproteasome and T helper cell differentiation in systemic Candida albicans infection

11. Emma Reeves - University of Southampton - The role of ERAP1 in HPV associated cancer 12. Efstratios Stratikos - National Center for Scientific Research Demokritos - Allele-dependent

activation of ERAP1 by a small molecule 13. Thorbald van Hall - Leiden University Medical Center - Involvement of several alternative antigen

processing routes for the MHC-I presentation of TAP-independent peptides 14. Sander van Kasteren - Leiden University - Tracking long-term antigenic fate using bio-orthogonal

chemical tags 15. Xiaoli Wang - Washington University School of Medicine - Modulation of antigen presentation by

rodent herpesvirus Peru 16. Jiajie Wei – NIAID/National Institutes of Health - Illuminating DRiPs with GFP 17. Emmanuel Wiertz - University Medical Center Utrecht - A genome-wide high-complexity shRNA

library screen identifies novel factors in MHC class I trafficking and degradation



Wednesday 6

POSTER SESSION 1 – North American Hall (continuned) 18. Najla Arshad - HHMI, Yale University School of Medicine - Studies on the peptide-loading

machinery of MHC class I molecules 19. Alistair Bailey - University of Southampton - MHC class I function is determined by protein

plasticity 20. Michal Bassani-Sternberg - Max-Planck Institute of Biochemistry - Expression and turnover of

proteins govern their sampling for HLA class I presentation 21. Gosia Garstka - The Netherlands Cancer Institute - The specificity of the first peptide selection

step by MHC class I molecules

22. Wilmon Grant - Oregon Health and Science University - HLA-E Dependent Antigen Processing at the Mycobacterium tuberculosis Phagosome

23. Anne Halenius - University Medical Center Freiburg - A novel MHC class I allele-dependent function of the HCMV immunoevasin US11 manipulates the ligandome of HLA-B molecules

24. Xiao He - University of Utah - Inhibition of HLA-DM function by a family of structurally related small molecule compounds

25. Clemens Hermann - University of Cambridge - Tapasin-related protein restricts the MHC class I peptide repertoire by enhancing peptide exchange

26. Wei Jiang - Stanford University - HLA-DM and HLA-DO: bi-directional regulation of function, tuned by pH

27. Sascha Kretschmann - University Hospital Erlangen - Indirect presentation of Y-chromosome antigen DBY requires protein structures outside of the T-cell epitope

28. Wenbin Ma - Ludwig Institute for Cancer Research, Brussels - Long-peptide cross-presentation by human dendritic cells occurs in vacuoles by peptide exchange on nascent HLA-I molecules that follow an alternative secretion pathway

29. David Margulies - NIAID/National Institutes of Health - Tapasin-related protein TAPBP-R interacts directly with peptide-free MHC-I/b2-microglobulin complexes and is released by high affinity peptides

30. Sebastian Springer - Jacobs University - Dipeptides exchange peptides on MHC class I molecules 31. Jacques Thibodeau - University of Montreal - The human invariant chain isoform p35 promotes

formation of nonameric complexes with MHC class II molecules and is needed for copII-mediated ER egress

6:00 DINNER - Academy Commons (1st Floor)

7:30 KENOTE 2 - Sponsored by The British Society for Immunology Alain Townsend, University of Oxford Active and passive immunity to pandemic influenza

8:30 BREAK (no refreshments)



Wednesday 7

SESSION 3: IMMUNOMICS CHAIR: Peter Kloetzel - Charité Universitätsmedizin Berlin


8:50 Emmanuel Wiertz - University Medical Center Utrecht - A genome-wide high-complexity shRNA library screen identifies novel factors in MHC class I trafficking and degradation

9:05 Joshua Elias– Stanford University - Unbiased proteomic – genomic investigation of MHC class I peptide ligands reveals unconventional translation origins

9:15 Arie Admon - Israel Institute of Technology - Defining the MHC peptidome production pipeline by stable isotope labeling and mass spectrometry based immunopeptidomics

9:25 Robbert Spaapen – Netherlands Cancer Institute - Identification of Novel Essential Regulators in the MHC class I Network

9:40 Kenneth Rock – University of Massachusetts Medical Center - Role of specialized proteasomes in thymic selection


Thursday, June 12, 2014

Thursday 8

SESSION 4: TRAFFICKING CO-CHAIRS: James Drake - Albany Medical College Anne Halenius - University Medical Center Freiburg

9:00 Organizational comments and Chair’s introduction

9:05 Jacques Neefjes – Netherlands Cancer Institute - Can we understand the motility of MHC class II compartments?

9:25 Paul Roche – NCI/National Institutes of Health - Ubiquitination by March-I Prevents pMHC-II Recycling

9:40 Willem Stoorvogel – Utrecht University - Whether MHCII-peptide complexes are expressed at the DC plasma membrane is determined at endosomes

10:00 Oddmund Bakke – University of Oslo - Invariant chain (Ii), a multifunctional molecule, its role in sorting and Ii as a vehicle for antigen loading onto MHC I and MHCII

10:15 Kai Wucherfennig – Dana Faber Cancer Institute/Harvard University- Mechanism of HLA-DM action in peptide loading and editing


11:00 Bénédicte Manoury – INSERM, U1013 Hôpital Necker - The MHC class II-associated invariant chain regulates TLR7 trafficking and signaling in B cells

11:10 Ana-Maria Lennon-Duménil - Institut Curie - Developmental Regulation of actin nucleation and dendritic cell migration

11:20 Jean Pieters - University of Basel - Activation of Macrophage and T cells—A Question of Survival

11:35 Satoshi Ishido – Showa Pharmaceutical University - Loss of MHC II ubiquitination induces negative consequence to dendritic cells

11:45 Nicolas Blanchard – INSERM, Université de Toulouse - Intracellular transport of Toxoplasma gondii antigens critically influences MHC class I presentation

11:55 Paul Lehner – University of Cambridge - Forward genetic screens in haploid human cells identify novel E3 ubiquitin ligases required for dislocation of MHC class I

12:15 -5:30 FREE TIME – ENJOY PHILADELPHIA



Thursday 9

5:30 POSTER SESSION 2 - North American Hall

1. Andrew Van Hateren - University of Southampton - The tapasin-related protein (TAPBPR) binds MHC class I molecules (MHC I) with high affinity and functions in a similar fashion to tapasin in vitro, suggesting TAPBPR is an additional MHC I peptide editor

2. Liusong Yin - University of Massachusetts Medical School - Susceptibility to HLA-DM is determined by a dynamic MHCII-peptide conformation constrained by interactions throughout the peptide-binding groove

3. Arie Admon - Israel Institute of Technology - Defining the MHC peptidome production pipeline by stable isotope labeling and mass spectrometry based immunopeptidomics

4. Teresa Ciudad - Universitat Autonoma de Barcelona - In vitro study of thyroglobulin peptides associated to HLA-DR3 under thyroid antigen presentation conditions

5. Joshua Elias - Stanford University - Unbiased proteomic–genomic investigation of MHC class I peptide ligands reveals unconventional translation origins

6. Sukrut Karandikar - University of Connecticut School of Medicine - Kb-restricted epitopes of ovalbumin

7. Elena Merino-Rodriguez - University of Massachusetts Medical School - A genome wide screen identifies a new peptidase involved in class I antigen processing and presentation

8. Robbert Spaapen - The Netherlands Cancer Institute - Identification of Novel Essential Regulators in the MHC class I Network

9. Philippe Benaroch - Institut Curie - Use of a novel synchronized protein trafficking system (RUSH) reveals a tight regulation of TLR3 exit from the ER

10. William Comrie - University of Pennsylvania - The dendritic cell cytoskeleton regulates ICAM-1 clustering and lateral mobility to promote T cell activation

11. Melanie Harriff - Portland VA Medical Center - Identification of key determinants of MR1 vesicular trafficking and antigen presentation

12. Satoshi Ishido - Showa Pharmaceutical University - Loss of MHC II ubiquitination induces negative consequence to dendritic cells

13. Ana-Maria Lennon-Duménil – Institut Curie - Developmental Regulation of actin nucleation and dendritic cell migration

14. Bénédicte Manoury - INSERM, U1013 Hôpital Necker - The MHC class II-associated invariant chain regulates TLR7 trafficking and signaling in B cells

15. Adriana Mantegazza - Children’s Hospital of Philadelphia - TLR-dependent phagosome tabulation in dendritic cells promotes phagosome cross-talk but is dispensable for MHC-II antigen presentation

16. Pam Wearsch - Case Western Reserve University - Bacterial membrane vesicles: New players in immune evasion and antigen processing pathways

17. Jeff Colbert - University of Massachusetts Medical School - Novel genes involved in MHC I antigen presentation in Dendritic Cells

18. Jens Dinter - The Ragon Institute of MGH, MIT and Harvard - Differential cross-presentation of HIV epitopes by dendritic cells and macrophages identifies variable intracellular degradation rates of HIV peptides

19. Nataschja Ho – Leiden University Medical Center - Differential and sustained cross-presentation by DC subsets in vivo



Thursday 10

POSTER SESSION 2 – North American Hall (continuned)

20. Anne Hosmalin - INSERM U1016 - Cross-presentation of antigens from live cells by dendritic cells isolated ex vivo

21. Jose Villadangos - The University of Melbourne - Impaired Antigen Presentation Following Systemic Inflammatory Response Syndrome (SIRS) is Caused by the Local Induction of a Paralysis Program in Developing Dendritic Cells

22. Matthias Zehner - University of Bonn - Antigen translocation into the cytosol for cross-presentation is mediated by Sec61

23. Margarita Del Val - Centro de Biología Molecular Severo Ochoa (CSIC-UAM) - The efficiency of antigen presentation in vitro correlates with the capacity to induce protection in vivo

24. Karen Hastings - University of Arizona College of Medicine Phoenix - GILT expression in medullary thymic epithelial cells is required for presentation of tissue-specific self antigens and deletion of autoreactive T cells

25. Valerie Herrmann - University of Konstanz - Immunotherapy of hormone resistant prostate carcinoma by therapeutic vaccination with PLGA-microspheres

26. José López de Castro - Centro de Biología Molecular Severo Ochoa - Peptide handling by HLA-B27 subtypes influences their biological behavior, association with ankylosing spondylitis and susceptibility to ERAP1

27. Sanda Remakus - The Research Institute at Fox Chase Cancer Center - Protection by memory CD8+ T cells is strongly affected by antigen expression levels

28. Rachel Roper - East Carolina University - Poxvirus A35 Protein Regulation of MHC Class II Antigen Presentation

29. Alejandro Sanz-Bravo - Centro de Biología Molecular Severo Ocho - Dominant effect of the R528K mutation over other ankylosing spondylitis-associated ERAP1 polymorphisms on promoting the selective destruction of HLA-B27 ligands

30. Weiming Yuan - Keck School of Medicine, USC - A unique human-like CD8αβ+ iNKT cells in a humanized mouse model

7:00 BANQUET DINNER – Dinosaur Hall


Friday, June 13, 2014

Friday 11

SESSION 5: CROSS PRESENTATION CO-CHAIRS: Sebastian Springer – Jacobs University Anne Hosmalin - Institute Cochin, INSERM U1016

9:00 Organizational comments and Chair’s introduction

9:05 Peter Cresswell – HHMI, Yale University School of Medicine - Mechanisms regulating MHC class I-restricted cross-presentation

9:25 Matthias Zehner – University of Bonn - Antigen translocation into the cytosol for cross-presentation is mediated by Sec61

9:40 Peter van Endert - INSERM U103 - Rab14+ Endosomes Regulate Phagosome Maturation and MHC class I Cross-presentation by Dendritic Cells

10:00 Brian DeHaven – Thomas Jefferson University - CD4+ T Cell Resposes to Poxvirus Infection are Driven Principally by MHC Class II Cross-Presented Antigen

10:10 Marianne Boes - University Medical Center Utrecht - Antigen-specific CD4+ T cells are required for efficient cross-priming of human CMV-specific CD8+ T cells in stem cell transplantation patients


10:55 Edda Fiebiger – Boston Children’s Hospital, Harvard Medical School - Dendritic cells use IgE-mediated cross-presentation to generate cytotoxic T cells in response to low dose soluble antigen

11:05 Jose Villadangos – The University of Melbourne - Impaired Antigen Presentation Following Systemic Inflammatory Response Syndrome (SIRS) is Caused by the Local Induction of a Paralysis Program in Developing Dendritic Cells

11:20 Benoit Van den Eynde – Ludwig Institute for Cancer Research, Brussels Branch - Long-peptide cross-presentation by human dendritic cells occurs in vacuoles by peptide exchange on nascent HLA-I molecules that follow an alternative secretion pathway

11:40 Hidde Ploegh – Whitehead Institute for Biomedical Research - New tools to study antigen processing and presentation

12:00 LUNCH - Academy Commons (1st Floor)


Friday, June 13, 2014

Friday 12

SESSION 6: RECOGNITION CO-CHAIRS: Lisa Dezin - Rutgers Robert Wood Johnson Medical School Pamela Wearsch – Case Western Reserve


1:05 Elizabeth Mellins - Stanford University - Crossreactivity between influenza hemagglutinin and hypocretin (HCRT) epitopes: a candidate trigger for immune dysfunction in narcolepsy

1:25 Victor Engelhard – University of Virginia - Induction of peripheral tolerance in CD8 and CD4 T cells by lymphatic endothelial cells

1:40 Andrea Sant – University of Rochester - Selection of immunodominant CD4 T cell epitopes in responses to peptide vaccination

1:55 Karen Hastings – University of Arizona College of Medicine - GILT expression in medullary thymic epithelial cells is required for presentation of tissue-specific self antigens and deletion of autoreactive T cells

2:05 David Lewinsohn – Oregon Health & Sciences University - MR1-restricted MAIT cells display ligand discrimination and pathogen selectivity through distinct T-Cell Receptor usage


2:40 Raúl Castaño – Universidad de Barcelona - CD1d restricted nonglycosidic iNKT agonists are potent stimulators of antitumor response: functional and structural characterization

2:50 Weiming Yuan – Keck School of Medicine, USC - A unique human-like CD8αβ+ iNKT cells in a humanized mouse model

3:00 Marcus Groettrup – University of Konstanz - The Ubiquitin-like modifier FAT10 Shapes the T lymphocyte Repertoire

3:15 Luis Sigal – The Research Institute at Fox Chase Cancer Center - Protection by memory CD8+ T cells is strongly affected by antigen expression levels

3:25 Emil Unanue – Washington University - Importance of endoplasmic reticulum stress in the generation of autoreactive peptides in NOD diabetes


4:00 KENOTE 3 Sebastian Amigorena – Institut Curie INSERM U932 How phagosome functions and cross presentation are controlled by innate sensing in dendritic cells

5:00 Closing Remarks – Laurence Eisenlohr, Thomas Jefferson University

Documents

8 International Workshop on Antigen Processing & Presentation...proteins govern their sampling for HLA class I presentation 21. Gosia Garstka -The Netherlands Cancer InstituteThe specificity