11
! / - ! !"#$%&'()*+ !" Scott A. Schlueter and James D. Hutchison, Jr Montana Department of Justice, Division of Forensic Science E N F!"#$%&'() !"#$%&'(#$%)*+!"# !" SJ !" !"#$%&'() !"# !"#$%&'(J!" = !"#$%&' xNz !"#$%&'() !"# !"#$%&'()*+,-+./0123 !"# !"#$%&'() !"# !"# J !"#$%&'()*+, !"#$%&'() !"# !"# !"#$%&'() !"#$%&'( !"#$%&'()!"#$%&'( !"#$%&'()*+,-./!" !"#$% J !"#$%&'() !"#$%&#'()*+ J ! ! !" ^ÖáäÉåí=NNMM ! L !"# !"#$%& !"# !"# !"#$ !"#$%& NNMMsi !"# !"#$EbpfF! =! !"#$%&'()*+!" ^ÖáäÉåí J !"#$%&'()*+,-

5988-4805(11p) · Scott A. Schlueter and James D. Hutchison, Jr Montana Department of Justice, Division of Forensic Science E N F !"#$%&'() !"#$%&'(#$%)*+ !"#!" SJ !"

  • Upload
    lehuong

  • View
    214

  • Download
    0

Embed Size (px)

Citation preview

�� ! /�� -�� !�� !"#$%&'()*+

��� !"

Scott A. Schlueter and James D. Hutchison, Jr

Montana Department of Justice, Division of Forensic Science

��

�� E�� N��F�� !"#$%&'()��

�� !"#$%&'(#$%)*+�� !"#

�� !" SJ�� !"�� !"#$%&'()

�� !"#�� !"#$%&'(J�� !"=�

�� !"#$%&'xNz��� !"#$%&'()

�� !"#

�� !"#$%&'()*+,-+./0123

�� !"#�� !"#$%&'()�� !"#

�� !"# J�� !"#$%&'()*+,

�� !"#$%&'()�� !"#�� !"#

�� !"# $%&'()�� !"#$%&'(

�� !"#$%&'()�� !"#$%&'(

�� !"#$%&'()*+,-./�� !"

�� !"#$%�� J�� !"#$%&'()

�� !"#$%&#'()*+J�� !

�� !

�� !"^ÖáäÉåí=NNMM�� ! L�� !"#�

�� !"#$%&�� !"#�� !"#��

�� !"#$�� !"#$%&�� NNMMsi�

�� !"#�� !"#$EbpfF�� !=�� !

�� !"#$%&'()*+�� !"^ÖáäÉåí

�J�� !"#$%&'()*+,-

�� !"#$% `Éêáä ä á~åí ��E�� o~Çá~å

^å~äóíáÅ~ä=mêçÇìÅíëF�� !�� !"#$%#&

�� !"#"$%&'()*+,-./0123

���� !"#$%&'()*+,-./012

�J�� !"#$

�� !"#$%&'()*+,-./�� !!

���� �PT�� ! NO�� �� �EN��F�

�� EN=ãÖLãäF���I��PM�� �� !"#$

�� O�� ée=V� NM=ãj�� !"# K�� !

�� I� PMMM=êéã�� NM��

�� !"E`b`NUN R S I = rå á í É Ç = `ÜÉã á Å ~ ä

qÉÅÜåçäçÖáÉëF�� O�� !"O�� !"#$�

�� O�� !"#$%&'(�� !"#$%&

�� !"#$%&'(�� !"#$%�� !

�� !

� 1. �� !"#$%&'()

�� ! 6-�� ! ��

�� �� ! �� !"#

�� !(��) ��

2

�� !"#$%&'( R�� �� P�� !"

�� !"#$�� !"#QM�� �� !"#�

��RM�� !"#$I=�� !N�� !"#$�

NRMMMêéã�� O�� NM�� !"#$J��

��

�� !"�� !"#$%&'��JPJ�� !

�� !JSJ�� !"#$%&'()�� ��

�� !"#$%&'()*+,-./0�� ��

�� !"#$%&'N�� !"#NMM�� !"

NMMM��L�� !EéeZOKRF�βJ�� !"#E�

�m~íÉää~=îìäÖ~í~�� !F�� !"�� !"#

�� !�� !"#$%&'()*+,-

EÄÉåòçÇá~òÉéáåÉFI�� !"#$%&'()*+,

��

�� !"#$�� !"#$%&'()'*+!

�� I�� L�� !"�� L�� !"#FI

�� !"#$%&'()*+,-.�� !"#

�� !"#$%&'()*+,-./0�� !

�� !"#$%&'(�� !"�� !"#$%

�� !"#$%&'(

�� !"#$%&'(�� !"#�� !"#$

�� !"#

�� !"

�� !"#$%&'(� SJ�� !" URB��

��NMMB��O�� !"#$%&'()*+,-

��

�� !

�� !

�� : Supelco Discovery HSC18;3 µm, 4.6 mm x 150 mm

�� : A=0.1%�� !"B= ��

�� : 0�� 5% B;2�� 5% B;10�� 90% B;20 �� 90% B

�� : 0.5 ml/min�� : 50��� !: 10 ��

�� !"#$%: �� ! 214 nm, 8;�� !: 360 nm, 100;

�� :�� (ESI); �� !"

�� !": 3000 V�� : �� , 13 L/min, 3500C�� : �� , 50 psi�� !: 120 V�� !: 100-650 amu��(stepsize): 0.1��(peak width): 0.12 ��

�� !(time filter): on�� !"#$%&': �� !(��): m/z 312 �� , �� !: m/z 286 �� /�� !"#: m/z 300 6-�� !: m/z 328 �� : m/z 298

3

�P�� !"#$%&'(ENMMM=åÖLãäF�� !"

�� �Q�� !"#$%&'$EORM=åÖLãäF��

�� !"#

�� !"#$%&'()�� !"MKMR=ãÖLãä�

MKTR=ãÖLãä��� !"#$I�� !"!#$%&

� 2. �� !"#$%&'()*+, .

�� !"#$%&'()*+'+,-.,EêOF��

MKVVK�� !=EåZNMF�� !"#$%&'()*+

�� MKOR=ãÖLi��� !N�� �� !"#$

� RB��� !"#$% RB��E�� Q�� !"

� NBF�

�� ! (��)

�� !��

��

�� !"#

6-�� !

��

4

� 3. �� !"#$%(1000 ng/ml)�� !"#$

�� !(��)

5

� 4. �� !"#$!(250 ng/ml)�� !"#

�� ! (�� )

�� !��

��

�� !"#

6-�� !

��

6

�R�� !"#$%&' (')*+,-�� !

�� !"# QU�� !"#$%�&�� !"#

�� !�� !"#$%&'()* aáä~çÇáÇ��

���� !"#$�� !"#$%&' MKUQ=ãÖL

i�� !"#$%&'MKMU=ãÖLi�

�� !"#$E��R��F�� !"#$%&'(

�� ! QM�� !"# I�� !"#$%&'(

�� !�� !"#�� !"#$%&"'()*

�� !�� !"#�� !"#$MKOP=ãÖLi��

�� !"#$%&'�� !"#$%&'()*

�EMKMR=ãÖLiF�

�� !"W=��QN�� !"#$%&'()*+,

�� !"#$%&'( I�� !"#$%&'(

���� !"��J�� !"#�� !"#MKMR

ãÖLiI�� !"SJ�� !"#$%&

�� !"#$!%"&'()*+,�� !"#

�� !"#MKMN=ãÖLiENMåÖLãäF�

� 1. �� !"#$%&'$% . �� !"#$%&' 0.25 mg/L

morphine hydromorphone codeine hydrocodone 6mam oxycodone

0.248 0.254 0.251 0.239 0.253 0.247

0.247 0.245 0.239 0.242 0.249 0.254

0.250 0.254 0.269 0.252 0.260 0.275

0.267 0.249 0.246 0.245 0.230 0.264

0.254 0.242 0.252 0.245 0.244 0.257

0.251 0.246 0.249 0.241 0.248 0.267

0.247 0.251 0.245 0.237 0.255 0.252

0.258 0.259 0.256 0.250 0.258 0.263

0.249 0.253 0.244 0.246 0.252 0.263

0.254 0.256 0.261 0.249 0.259 0.287

�� 0.253 0.251 0.251 0.245 0.251 0.263

�� ! 0.00942 0.00436 0.0128 0.00557 0.0128 0.0122

�� ! 3.729 1.737 5.102 2.276 5.117 4.638

�� !" 1.00% 0.36% 0.48% �2.16% 0.32% 5.16%1 Coefficient of variation = (standard deviation/mean) x 100;

2 percent error = (mean-target)/target x 100

7

� 5. �� !"#$%"#&'())*'+,-./

�� !(��)

�� !

��

8

� 6. �� !"#$%�� !"##$!%&

��

��

��

�� !

9

� 7. �� !"# , �� !6-�� !"#$$%"&'()*

�� !

��

��

6-�� !

10

www.agilent.com/chem

�� !"#$%&'()*+,-./01234�� !"#$%&'()*+,-.

�� !"#$%&'%(

�� !"#$%�� !"�� !"#$

�� !"#$%&'( ©�2001

2002 � 6 � 5�� !"

�� !5988-4805 CHCN

��

�� !"#$%��L�� J�� !"#$%&

�� !"#$%&'()�� !"#$%&'(

�MKMRJMKTR=ãÖLiI�� !"# !$%&'()*

�� !E�J��F�� �� !"#$%&'()

�� !"#$%&'()* pmb�� !"#$%

�� !"#�� !"J�� !"�J�� !"

�� �� !"i`Ljpa=si�� !"#$%&

�� !"�� !"#$%&#$'()I�� !

�� !"#$%&'() !*+,-./012

��

�� !

1. L.A. Broussard, L.C.Presley, T.Pittman, R.

Clouette, and G.H. Wimbish.Simultaneous

identification and quantitation of codeine,

morphine, hydrocodone,and hydromorphone

in urine as trimethylsilyl and oxime derivatives

by gas chromatography-mass spectrometry.

Clin.Chem.43,1029 –1032 (1997 )

2. Matthew H. Slawson, Dennis J. Crouch, David

M. Andrenyak, Douglas E. Rollins, Jeffry K.Lu,

and Peter L. Bailey, Determination of Mor-

phine, Morphine-3-glucuronide, and Morphine-

6-glucuronide in Plasma after Intravenous and

Intrathecal Morphine Administration Using

HPLC with Electrospray Ionization and Tan-

dem Mass Spectrometry. Journal of Analytical

Toxicology, 23, 468 –473 (1999 ).