李玉军

Experiment 3

化脓菌分离鉴定过程（三）

化脓菌分离鉴定过程

葡萄球菌• 甘露醇发酵试验• 血浆凝固酶试验• 耐热核酸酶试验• 动物试验

甘露醇发酵试验

Inoculate the bacteria into a mannitol micro-tube

Incubate at 37 for 18h. ℃S.aureus will ferment mannitol to produc

e acid, which causes the medium to turn yellow.

血浆凝固酶试验（ The Coagulase Test ） Coagulase

• 结合凝固酶• 游离凝固酶• 凝固酶 凝血酶样物质 纤维蛋白

It has been speculated that this enzyme might be a virulence factor. However, coagulase-negative strains are often as pathogenic as coagulase-positive strains.

There are two ways of performing this test • The Slide Coagulase Test• The Tube coagulase Test

协同因子 纤维蛋白原

The Slide Coagulase Test

细菌的药物敏感性试验

纸片扩散法（ K-B 法）

肠道菌分离鉴定过程（三）

Antigens of salmonella

O antigen polysaccharide of LPS used to divide Salmonella to 42 groups （ A － Z ， O51 － 63 ， O65 － 67 ） A-E groups are pathogenic

H antigen flagella antigen divide Salmonella tow phase: special no special

Vi antigen related to the virulence of Salmonella sensitive to heat ( lose activity at 60 )℃

Serological Identification of Salmonella

Select the specimen

React with A-Z polyvalent antiserum

React with several individual types of anti-O and anti-H

antiserum

React with anti-Vi antiserum

Agglutination reaction

( ＋ ) ( － )

Identify its group and phage

系统实验报告肠道菌化脓菌

目的策略（原理设计）方法步骤结果（判定方法）结论 讨论