PATENT ABSTRACTS

which bind an epitope on human CENP-B which corresponds to an epitope to which auto- antibodies to human CENP-B bind.

5196308

M E T H O D S F O R I D E N T I F Y I N G T H E D Q W 3 . 2 A L L E L E

A S S O C I A T E D W I T H I N C R E A S E D R I S K O F I N S U L I N - D E P E N D E N T

D I A B E T E S M E L L I T U S

Gerald Nepom, Barbara S Nepom, Tone, as- signed to Genetic Systems Corporation

Methods for identifying individuals at increased risk of diabetes are disclosed. The methods dis- closed utilize the discovery of the DQw3.2 variant, which identifies a specific allelic poly- morphism at a sinle gene locus. One preferred method utilizes a labeled probe to detect the DQw3.2 allele. This method involves estimating the size of the hybridizable DNA fragment gene- rated by a specific restriction endonuclease and therefrom determining the presence of the allele. A second preferred method involves the serologic detection of the DQw3.2 allele. Within this method, immunocomplexes formed between two different MAb's and separate portions of a cell collection are detected and the presence or absence of the allele determined.

119

5196318

P R E C I S E L Y R E G U L A T E D E X P R E S S I O N O F D E L E T E R I O U S

G E N E S

Thomas O Baldwin, Jerry Devine, Gerald S Shadel, Takarazuka, assigned to The Texas A&M University System

The invention relates to an expression vector system based on the regulation of bacterial luminescence (the lux gene system). The inven- tion further relates to the construction of a pre- cisely regulatable expression vector system which comprises a complete luxR gene in com- bination with an inactivated luxI gene. If the sys- tem is turned off, no significant transcription occurs of any cloned gene product when used in combination with the regulatory scheme of the invention as is demonstrated by using the bac- teriophage lambda lysis genes. The induction of transcription relies on the addition of exogenous autoinducer which is both inexpensive and easy- to-use and which is required in only minute amounts.

5196316

E N Z Y M E A N D D N A C O D I N G T H E R E F O R

Yasuno Iwasaki, Yoshik Nishikawa, Takarazuka, Japan assigned to Japat Ltd

The invention concerns a peptidylhydroxygly- cine N-C lyase (PHL) catalyzing the reaction See Patent for Tabular Presentation PS wherein R represents a peptide residue, and GIyOH represents an alpha-hydroxygiycine residue linked to the C-terminus of said peptide R by an amide bond, a recombinant DNA molecule coding for a PILL, a method for the preparation of such a recombinant DNA molecule, processes for the preparation of PHL from a natural source or by means of the said recombinant DNA molecule, and the use of PIlL for the pre- paration of an amidated peptide R-NH2.

5196319

P R I M A R Y B I L I A R Y C I R R H O S I S A U T O A N T I G E N

Ross Coppel, Merrill E Gershwin, Victoria, Austrafia assigned to Amrad Corporation Limited

ADNA molecule is provided which comprises a nucleotide sequence substantially corresponding to all or a portion of the base sequence coding for the mitochondrial autoantigen of primary bi- liary cirrhosis. This DNA molecule may be linked to an expression control sequence. A syn- thetic peptide or polypeptide is provided which includes at least an amino acid sequence substan- tially as shown in FIG. 6 or FIG. 8. This syn- thetic peptide or polypeptide may be prepared by expression of a host cell which has been trans- formed with a recombinant DNA molecule as described above, or by chemical synthesis.