104 PATENT ABSTRACI~

may carry an ice nucleation gene and the altera- tion of phenotype may be detected using an ice nucleation assay. By employing a panel of phage, unknown bacteria nmay be typed based on the pattern of reactivity observed. The transducing particles may be prepared by introducing a syn- thetic transposable element carrying the hetero- logous gene to a host carrying a prophage having the desired host range. After transposition, the host may be induced to a lytic cycle to release the transducing particles carrying the heterologous gene.

5187073

P R O C E S S F O R T R A N S F O R M I N G G R A M I N E A E A N D T H E P R O D U C T S T H E R E O F

5187075

C L O N I N G A N D E X P R E S S I O N O F A V A R I A N T G E N E O F P L A T E L E T F A C T O R 4 A N D C O M P O S I T I O N S

T H E R E O F T O M O D U L A T E I M M U N E R E S P O N S E S

Christopher Green, Paul H Johnson assigned to SRI International

The present invention provides for the purifica- tion from native sources or the cloning and ex- pression of a variant form ofplatelet factor 4 and also provides recombinant DNA vectors and methods for the expression and recovery of the platelet factor 4 variant. Also provided are com- positions and methods for modulating immune responses in mammals comprising immuno- modulating effective amounts of platelet factor 4 variant.

Stephen L Goldman, Anne C F Graves assigned to The University of Toledo

A method of producing transformed Gramineae comprising making a wound in a seedling in an area of the seedling containing rapidly dividing cells and inoculating the wound with vir + Agro- bacterium tumefaciens. Also, this same method wherein the ,dr+ A. tumefaciens contains a vec- tor comprising genetically-engineered T-DNA. There are further provided a transformed pollen grain of a Gramineae, a pollen grain of a Gramineae produced by a plant grown from a seedling infected with vir+ A. tumefaciens, a pollen grain o fa Gramineae produced by a plant grown from a seedling infected with v i r+A. tumefaciens containing a vector comprising genetically-engineered T-DNA, a pollen grain of a Gramineae whose cells contain a segment ofT- DNA, and Gramineae derived from each of these pollen grains. There are also provided a transformed Gramineae plant, a transformed Gramineae plant derived from a seedling in- fected with vir+ Agrobacterium tumefaciens, a transformed Gramineae plant derived from a seedling infected with vir+ A. tumefaciens con- taining a vector comprising genetically- engineered T-DNA and a Gramineae plant whose cells contain a segment of T-DNA. Finally, there are provided transformed Gramineae derived from seedlings infected with vir+ Agrobacterium tumefaciens and trans- formed Gramineae derived from seedlings in- fected with vir+ A. tumefaciens containing a vector comprising genetically-engincered T- DNA.

5187076

D N A S E Q U E N C E S E N C O D I N G B M P - 6 P R O T E I N S

John M Wozney, Elizabeth A Wang, Vicki A Rosen, Anthony J Celeste assigned to Genetics Institute Inc

Purified BMP-6 proteins and processes for pro- ducing them are disclosed. The proteins may be used in the treatment of bone and/or cartilage defects and in wound healing and related tissue repair.

5187078

P L A S M A - T Y P E G L U T A T H I O N E P E R O X I D A S E G E N E A N D

A P P L I C A T I O N O F T H E S A M E

Masami Ohya, Junz Mizoguchi, Takash Onozawa, Tomakomai, Japan assigned to Toyo Jozo Co Ltd

DNA having genetic information of a human plasma-type glutathione peroxidase h- p*GSHPx was identified and disclosed. The en- zyme, h-p*GSHPx, is produced by the