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Results and discussion
°
°
Conclusions
REFERENCES:
Human cardiac troponin T is cleaved
by thrombin in serum samples
4th Joint EFLM-UEMS Congress "Laboratory medicine at the clinical interface“, Warsaw, September 21-24th 2016, Poster 0193
Introduction
µ
µ
µ
µ
Methods
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1 MSDIEEVVEE YEEEEQEEAA VEEQEEAAEE DAEAEAETEE TRAEEDEEEE
51 EAKEAEDGPM EESKPKPRSF MPNLVPPKIP DGERVDFDDI HRKRMEKDLN
101 ELQALIEAHF ENRKKEEEEL VSLKDRIERR RAERAEQQRI RNEREKERQN
151 RLAEERARRE EEENRRKAED EARKKKALSN MMHFGGYIQK QAQTERKSGK
201 RQTEREKKKK ILAERRKVLA IDHLNEDQLR EKAKELWQSI YNLEAEKFDL
251 QEKFKQQKYE INVLRNRIND NQKVSKTRGK AKVTGRWK
TnT103 TnT175 TnT8
TnT199
TnT199BА
26797 Da
(69-288 aar fragment)
13384 Da
(doubly charged ion)
8926 Da
(triply charged ion)
7704 Da
(2-68 aar fragment)
3852 Da
(doubly charged
ion)
cTnT “-“ Pat1 Pat2 Pat3
(st) S P S P S P S P
66
45
30
20.1
14.4
kDa
Full-sized cTnT
29 kDa fragment
16-19 kDa
fragments
1 2 3 4 5 6 7 8 9
1 2 3 4 5 6 7 8 9 10 11
66
45
30
20.1
14.4
kDa
Full-sized cTnT
29 kDa fragment
16-19 kDa
fragments
TnT103 TnT175 TnT8 TnT313 TnT199
(55-64) (73-80) (85-95) (119-138) (275-288)
1 2 3 4 5 6 7 8 9 10
30
20.1
14.4
kDa
Full-sized cTnT
29 kDa fragment
