3M Clean-Trace SystemMore reliable ATP tests
Why are sensitivity and repeatability important? When an adenosine triphosphate (ATP) hygiene monitoring system has poor
sensitivity or repeatability, there is a substantial risk that the result provided does NOT truly represent the hygienic status of the
location tested and therefore provides false positives (leading to unnecessary chemical and labor costs and production delays) or
false negatives (leading to releasing a contaminated piece of equipment).
A system that is sensitive to low level contamination of a surface by microorganisms and/or food residues allows you to more
accurately understand the status of a test point. A test that will detect low level of contaminants will help ensure that you can
take the appropriate action i.e., positive release following a pass result, or a re-clean following a fail result. The ability of a
system to repeat results gives you peace of mind that the result is reliable and the actions taken are appropriate.
Greater sensitivity provides better information to help make the right decisions
An independent study conducted by Cara Technology Limited, Protocol for assessing the sensitivity of hygiene test systems for
live microorganisms and food residue1, compared the 3M Clean-Trace NG Luminometer and 3M Clean-Trace Surface ATP
Test System (formerly Biotrace Uni-Lite NG/Clean-Trace System) and Hygiena System SURE II ATP System. The study
tested each systems ability to detect a variety of microbes and food substances over a range of concentrations.
The study concluded, the Biotrace System has better sensitivity and repeatability for residues of food and live microorganisms,
and goes on to say, compared to the Biotrace equipment, the Hygiena System produced almost 60% false negatives based on
the samples tested. In the study, serial dilutions of microbial cultures, yeast extract, and yogurt were tested with both the 3M
Clean-Trace System and the Hygiena System using the manufacturers recommended pass/caution/fail limits. The average RLU
value and its interpretation, based on the manufacturers recommended pass/fail limits, for each dilution level is shown in the
chart below. The 3M Clean-Trace System showed much greater sensitivity to low-level contamination. As the chart below
indicates, in many of the dilutions, a FAIL result was obtained with the Clean-Trace System when a CAUTION or PASS
result was obtained by the Hygiena System.
Staphylococcus Citrobacter Zygosaccharomyces Yeast Yogurt aureus freundii bailii extract drink
Dilution 1 47282 197 51879 586 27556 467 1213 1 8162 17
Dilution 2 7969 22 8188 106 3401 41 272 2 3609 11
Dilution 3 880 4 999 21 448 8 69 2 596 19
Dilution 4 133 2 143 3 77 3 46 1 120 4
pass caution fail
3M 3M 3M 3M 3MClean-Trace Hygenia Clean-Trace Hygenia Clean-Trace Hygenia Clean-Trace Hygenia Clean-Trace Hygenia
Sensitivity Results of 3M Clean-Trace System compared to Hygiena System Sure II*
* Criteria for pass/fail limits were set at Pass: 150 RLU/Fail: 300 RLU for the 3M Clean-Trace System and Pass: 10 RLU/Fail: 30 RLU for the Hygiena System as per the respective manufacturers recommended pass/fail limits.Note the 300 RLU for the Clean-Trace FAIL level.
Not All Hygiene Monitoring Systems are Equal
03M Clean-Trace BioControl MVP NovaLUM/PocketSwab AccuPoint System System System System
Repeatability Score (%CV = Coefficent of variation)A lower score means superior repeatability
Repeatability Score (%CV = Coefficient of variation)
A lower score means a higher degree of repeatability/less variation in test results.
The 3M Clean-Trace NG Luminometer and 3M Clean-Trace Surface ATP Test
was the most repeatable
References: 1. Protocol for assessing the sensitivity of hygiene test systems for live microorganisms and food residue. W.J. Simpson, J.L Archibald,C.J. Giles. Cara Technology Limited, Leatherhead Enterprise Centre, Randalls Road, Leatherhead, Surrey, KT22 7Ry, UK Report 120906, 27 July 2006 2. The repeatability of hygiene test systems in measurement of low levels of ATP. W.J. Simpson, C.J. Giles, H.A. Flockhart. Cara Technology Limited, Leatherhead Enterprise Centre, Randalls Road, Leatherhead, Surrey, KT22 7Ry, UK Report 30606, 27 July 2006
3M, Clean-Trace, Biotrace and Uni-Lite are trademarks of 3M. Hygiena and Hygiena System SURE II are trademarks of Hygiena Inc. BioControl MVP is a trademark of BioControl Systems Inc. NovaLum and PocketSwab are trademarks of Charm Sciences Inc. AccuPoint is a trademark of Neogen Corporation.
3M Clean-Trace Luminometer and 3M Clean-Trace Data Trending Software
Please recycle. Printed in USA. 3M 2007. All rights reserved.MIC020 - 100770-2009-9345-2
3M Microbiology3M Center Bldg 275-5W-05St. Paul, MN 55144-1000USA1-800-328-6553
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Superior repeatability over other commercially available systems tested
In another study conducted by Cara Technology Limited,
The repeatability of hygiene test systems in measurement
of low levels of ATP 2, four commercially available ATP
bioluminescence systems were tested for repeatability of test
In the study, 30 tests were performed on the four commercially
available systems in accordance to the manufacturers directions.
To measure repeatability, the swabs were spiked with identical
amounts of ATP for each test reading. The study concluded the
3M Clean-Trace NG Luminometer and 3M Clean-Trace Surface
ATP Test was the most repeatable. This means you can be
confident the 3M Clean-Trace System is providing you with
reliable information to help you make the correct decisions for
your plant, whereas other systems may leave you guessing.
Your ATP testing MaximizedBy using the highly sensitive and repeatable system from 3M
Microbiology, you can be confident that the results you obtain
provide a clear picture of cleaning performance and the actions
taken on the basis of the results are sound ones that can save
your company time & money.
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Goldn Plump Poultry is the largest, fully integrated producer of chicken products in
the upper Midwest region of the United States. With distribution to grocery stores, delis
and restaurants as well as international exports, maintaining high quality standards is
essential to protecting their reputation and remaining successful in a competitive global
marketplace. Routine sanitation verification using an ATP testing system has been a
key component in Goldn Plumps quality assurance program over the last six years.
Sanitation is a key part of our total system. It affects product shelf life and
performance, says Renju Simon, Quality Services Manager at the Goldn Plump
Poultry facility in Cold Spring, MN. Approximately 500 pieces of manufacturing
equipment are utilized in our three plants. It is critical for us to have real-time data
about equipment cleanliness before we begin daily manufacturing. Previously,
when we sampled for microorganisms, we had to wait 24 to 48 hours for test results.
We need responses in real time so we switched to surface ATP testing to obtain
Despite the satisfaction that Goldn Plump had with ATP as a method for sanitation
verification, there were some drawbacks to the ATP testing system they were using.
The first ATP luminometer we used required manual calibration. We wanted
a more convenient instrument that could perform a self-check and quickly
be ready for use, said Simon. Our original ATP system also
categorized results as pass, caution or fail. We needed
numerical values for surface cleanliness to improve
our sanitation effectiveness..
The Goldn Plump Poultry team conducted background
research on three systems and viewed presentations and
demonstrations by each manufacturer.
We focused on features that went beyond numerical results,
said Simon. After narrowing our search to one system, we
tested it in our facilities. We evaluated the system for repeatability of
measurements as well as software compatibility with other Goldn Plump
Poultry systems. Ultimately, we chose the 3M Clean-Trace Surface ATP
System because it met the greatest number of our requirements and preferences.
The driving factor in our decision to choose the Clean-Trace system was the
3M Clean-Trace Data Trending Software, explained Simon. Our quality engineer
found it was easy to use and offered the most flexibility. He liked the open architecture
that enabled us to generate statistically sound sampling plans, randomly generate testing
sites and add extra sites on a daily basis that are not on the random schedule. It gave us
Not All Hygiene Monitoring Systems are Equal
Quality and Effi