Ultimate performance and flexibility

Designed to deliver the

highest performance

available in UV/Vis

detection, the 2489 UV/Vis

Detector offers:

■■ Uniquely designed optics

■■ Low detector noise

(<5 µAU)

■■ High-speed data rates

■■ Patented TaperSlit™

flow cell

■■ Maximum sensitivity

for HPLC and fast

LC separations

2489 UV/Vis DetectorAchieving high UV/Vis sensitivity shouldn’t be complicated

Set t ing t he Standard in hPLC det eCt ion

Whether you are doing routine UV-based applications or pushing

the limits of detection with low-level impurity analyses, Waters®

2489 UV/Visible (UV/Vis) Detector is the best detection choice for

performance, reliability, and usability.

The 2489 UV/Vis Detector is the most sensitive and versatile dual-

wavelength absorbance detector available for HPLC. The design combines

a high energy Deuterium source, a precise optical design, and low noise,

high speed electronics. These advanced capabilities take UV/Vis detection

performance to a new level.

trace impurity analysis

The detector’s extended linear range of 2.5 AU enables the

identification and quantitation of both major and minor components in

the same run – a key benefit for trace impurity or metabolism studies.

The ability to identify and quantitate low-level impurities in the same chromatographic run as the analyte of interest is a good measure of the detector’s sensitivity. The 2489 UV/Vis Detector easily integrates trace impurities down to 0.006% and lower.

2489 UV/Visible Detector.

Conditions:System: Alliance HPLC with 2489 UV/vis DetectorSample: 1.0 mg/ml irbesartan tabletColumn: XSelect™ HSS T3, 4.6 mm x 250 mm, 5 µmInjection volume: 8 µLFlow rate: 1.0 mL/min

Detection: 220 nm, 40 Hz, 0.05 s (normal)

AU

0.0000

0.0009

0.0018

0.0027

0.00 3.60 7.20 Minutes

10.80 14.40 18.00

Impu

rity

1 –

6.36

9

Impu

rity

2 –

9.28

7

Impu

rity

3 –

9.71

9

Impu

rity

4 –

107.

41

RCA

– 1

2629

Irbes

arta

n Ta

blet

– 1

4.90

4

Impurity Area %Signal-to Noise

Impurity 1 0.006 53

Impurity 2 0.011 70

Impurity 2 0.034 213

Impurity 2 0.003 18

RCA 0.018 89

Irbesartan Tablet 99.927 422602

Better analyte information

Dual wavelength capability is standard

on the 2489 UV/Vis Detector. This gives

you the flexibility to simultaneously

monitor absorbance at two user-selectable

wavelengths, resulting in more analyte

information per run and increased

productivity. The two wavelength channels can

be overlaid, ratioed, and/or summarized

within the data system for better

qualitative data.

Sample: Orange drink mix (unsweetened) 4.2 g in 250 mL of waterColumn: XBridge™ C18, 4.6 x 150 mm, 3.5 µm

Dual wavelength capability allows sensitivity and selectivity to be optimized for each analyte in a single run without the need for wavelength switching.

optimal performance

Whether you work in Research and

Development, Analytical Development,

or QA/QC, the 2489 UV/Vis Detector is

designed to meet your needs with its wide

range of flow cell options and integrated

features, while providing optimal

flexibility, usability, and performance for

both routine and complex samples.

Sample: 7 Ginseng std mix ~ 0.075 mg/mLColumn: XBridge Phenyl, 4.6 x 250 mm, 3.5 µm

As shown in this example, compounds found in natural products that exhibit weak UV absorption characteristics, such as ginsenosides found in Panax ginseng, are easily detected due to the superior sensitivity of the 2489 UV/Vis Detector.

0.00

0.01

0.02

0.03

0.04

0.05

0.06

0.07

22.0 24.0 26.0 28.0 30.0 32.0 34.0 36.0 38.0 40.0 42.0

AU

Minutes

Rg -

25.6

88

Rg -

26.5

11

Rg -

26.5

11

R1 -

35.1

91

R2 -

38.0

57

Rb2

- 39.

782

Rd -

41.4

44

0.00

0.20

0.40

0.60

0.80

1.00

yello

w 5

yello

w 5

red

40

red

40

0.00

0.05

0.10

0.15

0.20

0.25

0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00 11.00 12.00 13.00 14.00 15.00 16.00

AU

Minutes

440 nm

525 nm

FiLLed With innoVation – deSigned For USaBiLitY

Patented taperSlit flow cell*■■ Maximizes energy throughput

■■ Eliminates refractive index effects

■■ Maintains good peak shape, ensuring the highest

sensitivity while maintaining optimal performance

Lamp optimization■■ Designed to automatically maximize signal-to-noise in both the

visible and UV spectrums with the use of a single Deuterium lamp

■■ Extends the useful life of the lamp for consistent results over time

thermal wander management■■ Provides maximum baseline stability by managing the

effects of ambient temperature and humidity changes

■■ Minimizes vulnerability to hostile environmental conditions

■■ Reduces long-term baseline wander – especially important

for high-sensitivity analyses

high-speed data rates and independent filter optimization

The 2489 UV/Vis Detector offers a wide variety of sampling rates,

from 1 to 80 Hz, and independent optimization of filter constants.

These features better address the needs of today’s high-speed,

high-efficiency separations, while maintaining the sensitivity

you expect from quality UV detection.

Conventional cell

window

UV light

lens

TaperSlit™ flow cell

window

window

Fast LC separations with Intelligent Speed™ columns – Accurate integration of narrow, sharp peaks is assured with flexible data acquisition speeds.

Sample: 11 component standard, 20 µg/mL of eachColumn: Atlantis® dC18, 3 x 20 mm IS, 3 μmDetection: 254 nm, 80 Hz, 0.0125 s (fast)

AU

0.00

0.02

0.04

0.06

0.08

0.10

0.12

0.14

0.16

0.18

Minutes0.00 0.20 0.40 0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40

2-ac

etyl

fura

n - 0

.451

theo

phyl

ine

- 0.5

47

acet

anal

ide

- 0.6

60

acet

ophe

none

- 0.

914

buty

roph

enon

e - 1

.267

prop

ioph

enon

e - 1

.115 be

nzop

heno

ne -

1.33

1

vale

roph

enon

e - 1

.403

hexa

phen

one

- 1.5

29

hept

anop

heno

ne -

1.64

5

octa

nphe

none

- 1.

753

*Patent Number: 5,883,721 (U.S.)

0.04

0.03

0.02

0.01

0.00

0.04

0.03

0.02

0.01

0.00

0.00 1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00

Minutes

9.00 10.0011.0012.0013.0014.0015.0016.00

Rg -

26.5

11

Rg -

11.9

35

Ace

tam

inop

hen

- 3.2

04

Caffe

ine

- 11.

966

AU

New lamp < 2 hoursNoise = 4 µAU

Old lamp > 2,000 hoursNoise = 4 µAU

Lamp optimization ensures consistent noise performance for high sensitivity applications and reproducible integration whether your lamp is new, right out-of-the-box, or over 2000+ hours.

Sample: Caffeine and acetaminophen, approx. 0.0025 mg/mL Column: Atlantis dC18, 3.0 x 150 mm, 3 μmDetection: 270 nm, 5 Hz, 0.5 s

Waters Corporation 34 Maple Street Milford, MA 01757 U.S.A. T: 1 508 478 2000 F: 1 508 872 1990 www.waters.com

Waters, Alliance, Empower, MassLynx, and Atlantis are registered trademarks of Waters Corporation. Intelligent Speed, SunFire, TaperSlit, XBridge, XSelect, and The Science of What’s Possible are trademarks of Waters Corporation. All other trademarks are the property of their respective owners.

©2013 Waters Corporation. Produced in the U.S.A.February 2013 720002186EN VW-PC

Built-in compliance features■■ Patented** wavelength verification and calibration routine,

via integral erbium filter, enables you to set calibrations to within ±1 nm

ensuring wavelength accuracy.

■■ Integrated cuvette holder, coupled with Waters qualification workbooks

and optional cuvette kits, makes qualification easier and faster.

Simple performance maintenance

With the 2489 UV/Vis Detector, you can count on maximum

performance with minimum maintenance.

■■ Flow cells are easy-to-remove and completely interchangeable.

■■ The lamp is pre-aligned and front mounted for easy replacement.

■■ Lamp serial numbers, hours, and number of ignitions are

easily tracked.

int egrat ed SYSt eM or Stand-aLone oPerat ion

When operated as part of a Waters HPLC system, the 2489 UV/Vis Detector is

fully controlled via Empower® or MassLynx® software data systems, providing

intuitive data acquisition, analysis, reporting, and diagnostics tools.

As a stand-alone module, the 2489 UV/Vis Detector lets you manage both

primary and secondary detector functions using the detector’s intuitive front

panel keypad. Time-based programs can be constructed with full control of

major parameters and the detector’s on-board memory allows for the storage

of methods and/or spectra for easy retrieval.

Alliance® HPLC S ystem with a 2489 UV/Visible Detector.

**Patent Numbers: 6,423,249 and 6,783,705 (U.S.)

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CHooSe WATerS GLoBAL SerViCeS

Waters Global Services

focuses on optimizing

Waters products with

superior service, support,

upgrades, training, and

Waters Quality Parts.®

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to waters.com/services.