2.0 About Electrolab Biotech Ltd - ERT o cliente... · · 2015-11-162.0 About Electrolab Biotech Ltd ... design means that components can be easily substituted, ... it is important

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2.0 About Electrolab Biotech Ltd

Electrolab Biotech Ltd is a specialist manufacturer of bioreactor and associated equipment.

Established in 1989, the company has grown over the years to be one of the prime suppliers to the UK

and world academic markets.

As well as academia, Electrolab has also supplied into industry. Until the recent closure of the

department, Electrolab had what was probably the largest single installation of fermenters in a single

laboratory at an industrial site in the West, comprising of over 200 research systems at Pfizer,

Sandwich, UK (most of the equipment has subsequently been dispersed over a number of other Pfizer

sites in Europe and the US).

Electrolab has built on its reputation year on year, and has continually looked to improve and update

its product line. Starting with simple modular systems (where each element of the control was

performed by a separate unit), Electrolab has built up a line of reliable, industrial and rugged control

systems. We are continually looking to build and improve on these.

Supplying initially to the UK market only, export increased and now, on average, accounts for over half

of annual production.

Being a manufacturing company, Electrolab naturally understands the need for good support for its

customers. Electrolab has always been based in Gloucestershire in the UK and with close links to the

motorway network (we are just off junction 9 of the M5), we are able to travel to most of our UK

clients within a few hours. We have a dedicated team of service engineers, able to look after your

needs whether it is to repair your equipment or service and maintain it. Non-UK clients are supported

by a network of distributors as well as the UK team.


3.0 Electrolab Bioreactor System

3.1 Electrolab Bioreactor System, FerMac 320, Autoclavable

System overview description

The FerMac 320 is a modular bioreactor system, which can easily be adapted for the majority of research or production needs. Vessels are available as standard in 1L, 2L, 5L and 10L working volume, though we can produce some other sizes on request. The system is designed to take up the minimum of bench space (little over a metre), or can be mounted on an optional stand/trolley if so desired. The geometry of the vessels are designed as to be suitable for either cell or microbial culture, but the design means that components can be easily substituted, ensuring suitability for virtually all stir-tank batch, fed-batch and continuous cultures. All product contact materials are either electropolished 316L stainless steel (with a surface finish of 0.4 RA), borosilicate glass (the vessel itself) or Viton. The outside of the bioreactor headplate (top-plate) is also highly polished. The vessel is mounted in a tripod assembly. The vessel provided for microbial culture features a flat bottom. Each fermenter vessel is run by its own dedicated controller. This enables the systems to be run totally independently, and enabled them to be moved to different laboratories in the future if required. If a controller were to develop a fault, only one vessel is affected. The proposed system is configured to measure and control pH, dissolved oxygen, temperature and agitation, and foam/level control. Off-gas CO2/O2 analysis is available as an option. Control loops If pH falls beneath the desired setpoint, the controller activates a reagent pump to introduce a liquid base. If the process value is above the setpoint, the controller activates a reagent pump to introduce a liquid acid. A programmable dead band prevents over sensitivity and continued activation of the pumps. Alternatively, the PID terms can also be changed by the operator. When the measured dissolved oxygen value falls below setpoint, the first action of the controller can be configured to either add more air/oxygen, or increase the agitation speed (known as ‘stirrer in cascade’). If increasing agitation speed is not sufficient, a solenoid valve is opened to introduce more air into the vessel, until the desired value is achieved. This control loop also has operator-tuneable PID terms. Temperature is sensed by means of a Pt100 temperature sensor, located within a pocket and submerged in the process. When heating is required the controller activates the low-voltage wrap-around heater jacket. To maximise safety in the laboratory, the standard specification of heater jacket will not heat the vessel above 50oC, reducing the chance of accidental burns. Higher temperatures can be accommodated (up to 80oC) by use of a special heater jacket. If the desired temperature is exceeded, a solenoid valve is automatically opened to enable cooling water to circulate through the cooling coil. Again, the PID terms can be adjusted by the operator.


The agitation can be controlled by one of 2 ways – either by entering a manual agitation speed (with automatic control to set-point) or as part of the dissolved oxygen control loop. In this setting, the controller has the ability to ramp up the stirrer speed (up to an operator-set maximum), if it so requires. Foam is detected by a simple conductivity sensor. When foaming is detected, the integral antifoam pump is activated. You can adjust the on/off cycle of the pump, thus ensuring that there is sufficient time for the anti-foam to become properly mixed into the media. This prevents ‘over-dosing’ of anti-foam into the process, which can be detrimental to down-stream processing, and in severe cases affect sensor performance. Included in the package is Electrolab’s Fermentation Manager Software. This allows up to 8 Electrolab fermenters to log measured parameters, setpoints etc to a separate PC. Automatic time-based control aspects (ie pump activation) can also be conducted by the software. Runs from different fermenters can be logged independently, so they can be started at different times if required. Easy comparisons can be made with previous runs, and all data is exportable to MS Excel.

The System Components

The photo below is for representation only. The proposed vessel is an appropriately scaled version.

The separate gas service plate (rotameter plate) is not shown.


3.2 Fermenter Controller

Electrolab fermenter controller, model 360

The model 360 is a proven, versatile controller developed for use with

autoclavable bioreactors.

It is very user friendly and easy to operate. Operators can tell at a glance

the status of the various control loops. Any alarms are indicated by a

flashing warning light and audible beeper (which can be configured to be

silent if required).

The display provides clarity and ease of reading in most lighting

conditions – whether there is low light or sunlight falling across the

screen.

Main parameter control is via the rugged key pad interface. This enables

quick and easy access to all the main control parameters, as well as for

entering information such as set-points, starting/stopping control loops

etc.

For this proposal, the main control loops for each of the bioreactors are:

pH

Temperature

Dissolved oxygen

Foam/level control

Stirrer speed

Feed addition can be activated manually from the controller, or can be activated by the Fermentation

manager software if desired.

The control algorithm for pH, dissolved oxygen and temperature operate under PID terms. These can

either be left as per the factory settings or changed by the user.

The controller has password capability to prevent unauthorised access. The controller also

communicates directly with the Fermentation Manager Software (see section 4).

The installed input amplifiers have the following specifications: pH amplifier: Range: 0 - 14 pH Accuracy: ± 0.02 pH Temperature amplifier: Type: Pt-100 Range: 0 – 150oC Accuracy: ± 0.1oC


dO2 amplifier: Type: Polarographic Range: 0 - 150 % (air) Accuracy: ± 0.1 % Foam/Level amplifier: Type: On/Off signal Sensitivity Firmware selectable

View of the 360 interface

Connections on the 360 controller Connection of I/O and mains is at the rear of the controller Side connections:

sensor connections

pH : BNC

Temp : BNC

DO2 : 5-pin audio

foam/level : banana

RS232/RS422 connection to Fermentation Manager PC (if used)

Main power : 115V - 230V (UK standard 13 amp)

Expandability : space for additional 3 paramater cards on board

Pumps

As standard, a total of four 4 rpm pumps are mounted to the 360 controller. For the proposed application, their intended use would be as follows:

Acid addition (controlled by pH loop)

Base addition (controlled by pH loop)

Anti-foam addition (controlled by foam control loop)

Spare feed pump


The pumphead is a Watson-Marlow type common to many laboratory applications. To the right of each pumphead is a 3 function rocker switch, which has the following functions:

Auto Changes the pump mode so it is automatically controlled

Off Prevents the pump from activating, so ideal when changing tubing, addition bottles etc.

Prime Activates the pump manually, to enable feed lines to be primed. Alternatively the operator can use this function over and above the control screen to drive the pump for manual liquid additions.

Pump timer function If a pump is not allocated to a dedicated control loop (such as pH), then a timer function can be used. This enables the pump to be automatically stopped and started (the user programs the stop and run times) to introduce a feed at a pre-calculated addition rate. Please note that as the pumps are fixed speed, the flow is not continuous but pulsed. The stop/run times depend on the flow rate required and the bore size of the tubing used. If the Fermentation Manager Software is used (see Section 4), the feeds can be programmed to be initiated or stopped at user-defined times into the process run.


3.3 Gas Service Plate The gas service plate is provided with 1 rotameter for the addition of air (or oxygen enriched air), or for anaerobic processes it can be used for nitrogen. It enables a clear and simple visual overview of the gas flow at any given time. Flow rate is adjusted by means of a needle valve on the rotameter itself. When the process values of dO falls beneath setpoint, the controller activates a gas solenoid on the service plate, allowing the flow of gas into the vessel. A mass flow controller is available as an option – details on request. The service plate also handles the water connections to and from the condenser and heat exchanger. A cold water solenoid is also mounted on the service plate to regulate the flow of water to the heat exchanger on demand.

Please see Section 5.2 for details of optional Mass Flow Controller

Gas ‘Service Plate’ connections: Gas connections

Air or Air/O2 (2 barg) : 6 mm Festo quick connector

Water connections

Water inlet (max 2 barg) : 1x 6 mm Festo quick connector

Drain : 1x 6 mm serrated hose barb


Gas flow Rotameters

Gas flow is regulated by rotameters, which are mounted on the service plate. These assemblies are used to measure and adjust the gas flow rates. The gas enters the tube from the bottom, causing the float to move upwards to a point on the scale which corresponds with the flow rate. It then exits the tube at the top and is sent off to the bioreactor. The rotameters used have an easy to read long scale 140mm and are in direct read format. As rotameters are designed to work at certain pressures, it is important to note that any major deviation in the input pressure may result in a less accurate reading. Flow specification can be specified by you, but the following is recommended: Air rotameter ranges provided depend on bioreactor size:

1-13 L/min (for 10L bioreactors).

Other flow rates are available.

Please note: Other flow rates and additional rotameters are available as an option.


3.4 The Bioreactor 3.4.1 The Vessel The bioreactor vessel is fabricated in the UK from 316L stainless steel and borosilicate glass, and all metalwork has an internal surface finish of 0.4 RA. Headplate o-ring is Viton.

The vessel is designed with a height: depth ratio of approx. 2:1 at maximum working volume, making it ideal for microbial culturing. However, it can also easily be adapted for use with other cell lines (including mammalian) if required. The headplate is equipped with a number of threaded ports, enabling the vessel to be customised to the operator’s needs. However, the design of the ports means that there are no threads on the underside of the headplate that could prove difficult to clean. The vessel is supported in a tripod assembly made from anodised aluminium.

3.4.2 Bioreactor Headplate The bioreactor headplate is secured to the vessel by 6 headplate bolts. This ensures tight and even sealing, ensuring the risk of contamination is minimised. The headplate contains a number of ports to house the various fittings. There is 1 port on the headplate that has a fixed, pre-defined usage - the central port is taken up by the stirrer shaft. The other ports are either 6.35mm or 12mm diameter, and enable various configurations to be made using different fittings.


Headplate configuration, 10L microbial

Please note: a foam probe is included, and will be fitted to one of the ports above


3.4.3 Agitation Driveshaft and impellers The driveshaft is mounted in the central port of the headplate. The sealing is via mechanical seals. Shaft diameter is 10mm. The top of the shaft is fitted with a collar (not shown), which locates with the bayonet fitting on the motor. This facilitates self-aligning, enabling easy fitting of the motor.

Motor Motors used are high torque, but low voltage (24V) for safety. Feedback to the controller ensures that an accurate speed is maintained, even at low rpms. Speed can be adjusted from 50-1200 rpm (<10L) or 50-900 rpm (10L or above). The motors are fitted with a bayonet cap to ensure easy location on the driveshaft. Cooling loop and baffle, combined

For microbial applications a cooling loop with 2 integrated baffles is provided. The loop is connected to the service plate, and when the process temperature exceeds set-point, the controller automatically opens a solenoid valve, allowing water to circulate through the cooling loop.

Heating A low voltage (24V) wrap-around heating blanket is provided to ensure even, gentle heating for the process. This is automatically powered by the controller when the process value falls below setpoint. To reduce the risk of accidental burns, the maximum vessel temperature achievable is 50OC. For higher temperatures, and optional high-temperature upgrade is available.

Two 6-bladed Rushton-type impellers are supplied (other types are

available) for microbial culture. Impeller diameter ~ 1/3 vessel

diameter.


3.4.4 Aeration

Sparger and headspace The Gas Service Plate directs the gasses to the bioreactor. Configuration of the gas flow. The sparger is an L-type design, which enters the vessel via the headplate and continues down the inside of the vessel. Different designs of sparger are provided, depending on the process type. The end bends under the impeller shaft and for microbial applications the standard sparger has a series of 1mm diameter holes drilled on the underside of the sparger. Alternatively, a version is available which accepts a multi-hole tip (5x 1mm diameter holes), or various poral (sintered) tips. Offgas condenser Process gas is first cooled by means of a condenser before it leaves the bioreactor, via the offgas filter. Cooling water is circulated continually around the jacket to the condenser. The condenser contains a spiral baffle to maximise the surface area for any vapour to condense on. A PTFE hydrophobic 0.2 micron filter is fitted to prevent contamination of the bioreactor. An optional off-gas analyser for CO2/O2 (Electrolab Fermac 368 analyser) can be fitted to the exhaust line if desired.

3.4.5 Additions and dip tubes 3-way inlet Additions can be made to the bioreactor via two different methods. The first is via a tubing connection into a suitable port fitting (such as the 3-way adapter) This method is also employed for reagent addition. The reagents in their respective bottles are first autoclaved, having been prior connected to the feed line (tubing) and septum needle. The tubing is clamped off and the needle covered in foil. The bottle can vent in the autoclave by means of a 0.2 micron lab filter. Once the vessel has also been sterilised, connection can be made.

Inoculation port The second method is to use a needle through a septum, as with the inoculation port. A protective cap is fitted to protect the septum when not in use.

Dip tubes

The dip tubes have a 4mm diameter bore, and are height adjustable. A short (can

be used for additions) and a long dip tube (used as a sample pipe) are supplied.


3.4.6 Sample device for universal bottle Samples can be taken at any point during the process run. A sample system is provided, which accepts universal bottles. The sample device is connected to a sample pipe, which is fitted via the headplate. The sample pipe has a 4mm diameter bore. The sample device is designed to accept universal bottles – though we also produce versions for use with Falcon tubes and bijou bottles. A syringe is used to facilitate the withdrawal of the sample. The sample device comes with all necessary tubing, clamps, bottles and filters to enable samples to be taken with minimal risk of contamination of the bioreactor.


3.4.7 Sensors pH

A gel-filled pH sensor is provided for use with the system. As insertion is via the headplate, the length of the sensor used depends on the bioreactor size. The sensor is 12mm diameter, able to be sterilised in-situ or by autoclaving. The pH sensor sends a mV reading to the controller, corresponding to the pH value measured. The standard pH sensor offered is the Broadley James F695 series, which features the industry standard ‘’K9’’ style connector cap.

Specifications: • 0 - 13 pH range • Steam sterilizable to 135° C • 150 psig max. pressure • Double junction, Ag-AgCl reference system • Metric Pg13.5 threaded disconnect cap

Dissolved oxygen

A polarographic dO sensor is available as an option for use with the system. As insertion is via the headplate, the length of the sensor used depends on the bioreactor size. The sensor is 12mm diameter, able to be sterilised in-situ or by autoclaving. The dO sensor sends a mA reading to the controller, corresponding to the dO value measured. The OxyProbe 12 mm DO sensor has the same rugged performance as the 19 mm and 25 mm sensors. This polarographic sensor utilizes the longer silver anode and small cathode which has become the industry standard. It also uses the same semi-permeable, Silicon/Teflon composite membrane, reinforced with stainless steel mesh.

The standard dO sensor offered is the Broadley James D340 series, which features the industry standard ‘’K9’’ style connector cap. Temperature

The Pt100 temperature sensor fits into a thermopocket, via the headplate. Connection is directly to the fermenter controller.

Foam/Level Sensor A conductivity foam/level sensor and cable is supplied, which is height adjustable. The sensor is normally set approximately 1/3 into the available headspace, and should foam make contact with the sensor a circuit is completed. This in turn activates a pump on the 360 controller to dose in anti-foam agent as required. When running in level mode, the same pump will continue to add fresh media until the sensor detects the liquid level.


4.0 Fermentation Manager Software

Electrolab Fermentation Manager software is a Labview based program, developed exclusively for use with the Electrolab FerMac 320 and FerMac 360 range of bioreactor systems. Each copy of software can be connected with up to 8 bioreactor systems, but also enables the operator to retain independent control of each individual system. It is not a requirement to use this software, as the bioreactor systems can be operated without it. The software can be used either as a datalogger (with all data capable of being exported in .CSV format into programs such as MS Excel), or it can also be used to pre-program time based actions, such as changes to setpoints, activation and speed-control of peristaltic pumps. The front-page allocated to each system provides a simple overview, and plots a trend chart of all measured (and user-selected) parameters. Each parameter is individually colour coded to enable clear and easy identification on the graphs. Individual parameter alarms can be set, acknowledged and automatically recorded in the log. Programming of time-based actions is achieved by simple selection from drop-down menus, eliminating the need for entering complex commands manually. Off-line notes can also be added by the operator at any point during the fermentation run, with each one being automatically time-stamped.

Importantly, this software is license-free. There are no software or hardware keys required to operate this software, so users are free to load the software on their office or home computers to view data and compare previous runs. Within the software graphing package, you can call up the plots of multiple previous runs and overlay them on the same graph, enabling quick and easy comparison. The graph screen-shot facility enables easy integration of presentation data into reports.

The program is simple to understand and operate. It runs on Windows XP or 7. All communications cables, plus the appropriate USB converter, are supplied with the software. Should the software disc become lost, it can be downloaded from the internet (e-mail [email protected] for the link).

mailto:[email protected]


5.0 Options

5.1 Start-up kit The Start-up kit helps to provide the other basics required for a successful fermentation run. The individual components can also be purchased from Electrolab Biotech Ltd as spares. The start-up kit contains:

3x feed bottles

2x inlet (sparger) filters

2x exhaust (condenser) filters

2x tubing thumb clamps

Pump tubing, 3.2mm bore

Exhaust filter tubing

Water connection tubing (for condenser and heat exchanger)

5.2 Mass Flow Controller A thermal mass flow controller (MFC) is available as an option. It is operated by the dO control loop function of the fermenter controller. The MFC is set using the fermenter controller key-pad interface, and is mounted to the back of the service plate. As the desired maximum flow range is factory set, this must be determined before the equipment is delivered. The MFC is from the MediFlo range, manufactured by Pneucleus.


6.0 Basic Components List

FERMAC 360 ELECTRONIC MEASUREMENT & CONTROLLER Microprocessor monitoring and control system fitted with pre-amplifiers for measurement and control of temperature, pH, dissolved oxygen and agitation, and two additional feed pumps. The unit is supplied complete with agitation motor, all cables, 4 peristaltic pumps and RS232/485 port. A Pt100 temperature sensor and wrap-around heater jacket is also provided. A service plate with 1 rotameter, 1 gas solenoid valve and 1 cold-water solenoid valve is also provided. FERMAC VESSEL FOR MICROBIAL CULTURE 10 Litre working volume (total volume 12.3 Litres), flat bottomed borosilicate glass vessel with a stainless steel top plate.

The vessel is complete with: Interchangeable stirrer shaft with two Rushton-type impellers Gas sparge tube, l-type Long & short dip tubes Thermowell Cooling coil and baffles, combined Spare port blanking plugs Off-gas condenser 3-way reagent inlet Inoculation port, septum type pH sensor, sensor holder and cable dO sensor, sensor holder and cable Wrap-around heater jacket, 24V Sample device for universal bottles Foam sensor & cable

FERMENTATION MANAGER SOFTWARE (see section 4) For use on Windows XP or 7. OPTIONAL PARTS (see section 5)

5.1 Start-up kit 5.2 Mass Flow Controller


7.0 Vessel Dimensions

These should be taken into consideration to ensure your autoclave is large enough to accommodate the

required vessel. Dimensions for common vessel sizes are listed on the table below.

If a flexible condenser coupling (optional extra) is used, the overall height can be reduced by 100mm.

Vessel volume Diameter/mm Height/mm

0.8L/1L 205 307 2L 205 377

3L 246 475 5L 246 575

10L 290 650

15L/16L 310 672 18L 310 687

8.0 Utility Requirements

The following are required for the operation of each fermenter system.

1x domestic power supply (230 VAC / 110 VAC as appropriate)

All gases clean and dry, regulated to 0.5 barg

Water supply, regulated to 1 bar, and suitable drain (or a recirculating chiller).

Associated equipmentWith our in-depth knowledge of fermentation processes, we have developed the following associated products:-

The FerMac 368 Gas Analyser measures oxygen and carbondioxide in exit gas, two important extra parameters which givethe best indication of growth within a fermenter vessel.The Gas Analyser connects to the FerMac 360 Controllerso that measurement canbe logged and controlledby our software.

The Electrolab Low Flow Rate Pump is specifically designedfor bioprocess applications, providing a smooth flow of mediawith excellent reproducibility over extended periods of time.

The Fermac 366 Pump Module makes two extra pumps availablefrom the Fermac 360 controller. They can be used as level or feed

pumps and can be controlled via oursoftware, allowing complex feedsto be programmed.

Electrolab Biotech LtdUnit E2, Northway Trading Estate,Tewkesbury, Glos GL20 8JH, UKtel: +44 (0) 1684 291007fax: +44 (0) 1684 291006email: [email protected]

OUTLINE SPECIFICATION

To discuss your specific requirements, arrange a demonstration or obtain furtherinformation and pricing, contact us:

Vessels Bacteriological Cell Culture Air LiftWorking volume(Litres) 2 5 10 2 5 10 8

Total volume(Litres) 2.7 6.4 12.4 2.6 6.3 12.3 10

6.3mm Ports 6 5 7 8 7 9 612mm Ports 5 7 7 5 7 7 725mm Ports N/A N/A N/A N/A N/A N/A 4Impellers 2 x 55mm 2 x 55mm 2 x 55mm 1 x 50mm 1 x 62mm 1 x 74mm N/A

AgitationSpeed Range(rpm) 0-1000 0-1000 0-1000 0-800 0-800 0-800 N/A

Low Speed Option N/A N/A N/A 0-200 0-200 0-200 N/A

Temperature ControlHeating Pt100 sensor to measure vessel temperature & low voltage

(24V) wrap-around heating system

Cooling Integrated cooling coil & baffle Optional cold finger heat exchange

Range From 5°C above cooling water temperature to 50°C

pH Control Using autoclavable pHelectrode, controlled by additionof acid or base using 2 WatsonMarlow peristaltic pumps

Using autoclavable pHelectrode, controlled by CO2gas flow

Asbacterio-logical

Range 0-14 pH with on-screen calibration and electrode monitoring

DO Control Using autoclavablepolarographic DO electrode,controlled by either stirrer speed,air flow or a combination of both

Using a gas flow controllerwith air, O2 , N2 and CO2

Byair flow

Range 0-120% with on-screen calibration and electrode monitoring

Foam Control Using either conductivity probe or timer with a variable sensitivity.

Feed Pump Fully adjustable, using on and off timers. Maximum flow 6.4 mls/min

Power 230 volts, 50 Hz OR 115 volts, 60 Hz - Typically 1 kW

Software SCADA type software able to control and data-log up to 8 fermenters witheither RS232 or RS485 for multiple installations

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ERT Morada

FerMac 320The FerMac 320 is a medium-priced bioreactorfrom Electrolab introducing a new range of vesselscombined with a variation of our established 360control system.

This unique combination results in a trulyoutstanding value-for-money packagewhich offers a very compact, effective unitwith proven reliability.

With an expanding range of vessels anda control system pre-configured to

allow extra parameters to be addedat a later date, the FerMac 320

is the obvious choice to meet your laboratory's needsboth now and for the future.

www.electrolabtech.co.uk

FerMac 360 ControllerThe FerMac 360 has an intuitive easy-to-use interface where all your keyparameters can be displayed on a single screen and the set-up& calibration for each parameter is available with a single key stroke.

Spare parameter channels allow easy upgrades for redox, opticaldensity, CO2 etc - almost any measurable probe can be added at a laterdate. In addition, the FerMac 360 has links to our purpose designeddata-logging and control fermentation management software.

The FerMac 360 has bbuuiilltt--iinn motor-drive eliminating the need for an externalmotor-drive module and resulting in an extremely compact, efficient unit.

As standard, the unit is supplied with 4 Watson Marlow easy-to-loadpumps, all with manual over-ride facility. However, with the addition of ourFerMac 366 Two-pump Module, this can be increased to a total of 6. The FerMac 360 is pre-configured to take this and other modules such as our FerMac 368 Gas Analyser.

FerMac 320 AgitationThe FerMac 320 agitation system is equipped with a powerful direct drivemotor carefully designed with a locking system to ensure that the motorcannot be accidentally removed from the vessel whilst being easy toremove with a simple twist.

Uniquely, the locking system also tightens as speed is increased to give a positive, vibration-free drive at all speeds.

For cell culture work, the option of a very low speed motor gearbox is available.

FerMac vesselsThe FerMac 320 range of vessels allincorporate the same high quality materialsand design as other FerMac vessels. The full thickness top plate of 316L stainless steel hasall ports machined within it so that O-ring seals aremade on the sterile side of the vessel to reducecrevices or cross-over contamination. The ports areavailable in two sizes to take all standard probes andfittings for sampling, inoculation, addition etc and,within each size, are completely interchangeable.

An integrated twin baffle and cooling coil attached to thetop plate allows quick removal for easy cleaning.

Bacteriological VesselsThe aspect ratio of the FerMac 320 vessel is optimised forbacteriological use. In addition, maximum oxygen transferis achieved by combining two Rushton Turbine impellerswith flat-bottomed glassware.

Cell Culture VesselsSpecially designed for both mammalian and insect cellculture, the FerMac 320 glassware has a dish-shapedbottom for use with our low shear cell culture impellers.These are available in a wide range of sizes in bothup-draft and down-draft configuration. We also have a range of variable pitch cell culture impellers whichcan be useful for experimenting when dealing withdifficult mixing situations.

Our sparge tube is the newly designed cylindricalstainless steel type with porous tip to give maximumgas transfer.

Air Lift VesselsThe Electrolab Air Lift bioreactorprovides an excellent alternativeto the standard stirred vesselsystem. Agitation by air liftensures minimum shear stresswhilst allowing very high oxygentransfer rates. It can used forboth bacteriological work and cell culture as well as manyspecialist applications.

FerMac 320 Services PlateThe FerMac 320 is supplied with a separate free-standing services plate which ensuresthat water and gases are safely segregated from the electronics.

It comes complete with a precision flow meter for air and, (for cell culture work), O2 , N2and CO2 . Each flow meter has "easy-change" flow tubes to allow the gas flow range to be altered to suit your individual requirements.

The FerMac 320AIn addition to our existing extensive range of vessels, we cannow offer the FerMac 320A. This vessel was designed to offera real alternative to users of Applikon® systems who may feelthey are locked-in to one supplier when it comes to adding to their capacity.

The FerMac 320A offers the same vessel characteristics asoriginal Applikon® vessels with full interchangeability of partscombined with the high quality of all Electrolab vessels.

SoftwareEvery FerMac 320 system is complete with our easy-to-use SCADAsoftware. This versatile package, based on National Instruments Labview®,allows both data-logging and control of up to 8 bioreactors at one time andhas the ability to compare past and present fermentation runs.

Supplied licence-free, allowing data to be viewed on other computers eitherat home or in the office, it also has a built-in simulator - a combinationwhich is ideal for training purposes.

The data is Microsoft® compatible ensuring easy export into Excel or Word.

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2.0 About Electrolab Biotech Ltd - ERT o cliente... · · 2015-11-162.0 About Electrolab Biotech Ltd ... design means that components can be easily substituted, ... it is important