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Experimental Design and Observations
10. BIBLIOGRAPHY
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clxxvi
Experimental Design and Observations
APPENDIX – I
Composition of media
(A) SOLID MEDIA
Sr. No. Name of Solid Media Components Quantity (g/l)1) Yeast Extract Agar Yeast Extract
Malt Extract
Dextrose
Agar
DW
4.0
10.0
4.0
5.0
1 l2) Wheat Grain Agar Crushed wheat grain
Agar
DW
40.0
20.0
l l3) Coriander Agar Coriander seeds
Agar
DW
10.0
20.0
1 l4) Potato Dextrose Agar Peeled potatoes
Dextrose
Agar
DW
200.0
20.0
20.0
1 l5) Malt Extract Agar Malt extract
Agar
DW
25.0
20.0
1 l
6) Wood Extract Agar Wood chips
Dextrose
250.0
20.0
clxxvii
Experimental Design and Observations
Agar
DW
10.0
1 l7) Yeast Glucose Agar Yeast extract
Glucose
Agar
DW
4.0
20.0
15.0
1 l8) Carrot Agar Grated carrot
Agar
DW
20.0
20.0
1 l9) Czapek Dox Agar NaNO3
K2HPO4
MgSO4. 7H2O
KCl
FeSO4 . 7H2O
Sucrose
Agar
DW
2.0
1.0
0.5
0.5
0.01
30.0
15.0
1 l10
)
Maize Extract Agar Crushed maize grains
Agar
DW
40.0
20.0
1 l11
)
Dimmick Medium Glucose
K2HPO4
NaCl
MgSO4.7H2O
NaNO3
Agar
DW
10.0
0.5
0.5
0.2
2.0
15.0
1 l12 Pea Extract Agar Crushed pea seeds 40.0
clxxviii
Experimental Design and Observations
Agar
DW
20.0
1 l13
)
Yeast Potato Dextrose Peeled potatoes
Yeast extract
Dextrose
Agar
DW
250.0
1.0
20.0
20.0
1 l14
)
Potato Carrot Agar Potato
Carrot
Agar
DW
20.0
20.0
20.0
1 l15
)
Gram Extract Agar Gram
Agar
DW
40.0
20.0
1 l
(B) LIQUID MEDIA
Sr. No. Name of Liquid Media Components Quantity (g/l)
1) Richard's Solution KNO3
K2HPO4
MgSO4.7H2O
FeCl3
10.0
5.0
2.5
0.02
clxxix
Experimental Design and Observations
Sr. No. Name of Liquid Media Components Quantity (g/l)
Sucrose
DW
50.0
1l2) Glucose Asparagine Medium Glucose
Asparagine
MgSO4.7H2O
KH2PO4
DW
30.0
1.0
0.5
1.5
1 l3) Maltose Peptone Broth Maltose
Peptone
DW
3.0
1.0
1 l4) Glucose Peptone Medium Glucose
Peptone
DW
5.0
20.0
1 l5) Peptone water Peptone
NaCl
DW
10.0
5.0
1 l
6) Asthana & Hawker Medium KNO3
KH2PO4
MgSO4.7H2O
Glucose
DW
3.5
1.75
0.75
5.0
1 l7) Koser Citrate Medium NaCl
MgSO4.7H2O
5.0
0.2
clxxx
Experimental Design and Observations
NH4H2PO4
K2HPO4
Citric Acid
DW
1.0
1.0
2.0
1 l8) Dimmick Medium K2HPO4
NaCl
MgSO4.7H2O
NaNO3
Glucose
DW
0.5
0.5
0.2
2.0
10.0
1 l9) Czapek Dox Medium NaNO3
K2HPO4
KCl
MgSO4.7H2O
FeSO4
Sucrose
DW
3.0
1.0
0.5
0.5
0.01
30.0
1 l10) Yeast Glucose Medium Yeast extract
Glucose
K2HPO4
MgSO4.7H2O
DW
4.0
20.0
1.0
0.5
1 l11) Potato Dextrose Broth Peeled potatoes
Dextrose
NaCl
DW
500.0
10.0
1.0
1 l12) Pea Broth Dried Peas 150.0
clxxxi
Experimental Design and Observations
DW 1 l13) Rye Seed Medium Rye Seed
Dextrose
DW
200.0
10.0
1 l14) Malt Broth Malt Extract
Dextrose
Peptone
KH2PO4
MgSO4
DW
5.0
15.0
1.0
1.0
0.5
1 l15) Will Mineral Salts Medium KCl
KH2PO4
MgSO4.7H2O
K2CO3
DW
0.05
0.10
0.05
0.30
1 lAPPENDIX – II
Citrate Buffer
1) 0.1 M solution of Citric acid 21.06 gm Citric acid in 1000 ml of distilled water
2) 0.2 M solution of Sodium citrate 29.41 gm Sodium citrate in 1000 ml of distilled water
Prepare 100 ml by adding double distilled water
Sr. No.
pH Citric acid (ml) Sodium citrate (ml)
1 3.0 46.5 3.5
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Experimental Design and Observations
2 3.5 40.0 10.03 4.0 33.0 17.04 4.5 28.0 22.05 5.0 20.5 29.56 5.5 16.0 34.07 6.0 9.5 41.5
APPENDIX – III
Phosphate Buffer
1) 0.2 M solution of Monobasic Sodium phosphate
27.8 gm in 1000 ml of distilled water
2) 0.2 M solution of Dibasic Sodium phosphate
53.65 gm in 1000 ml of distilled water
2) Na2HPO4.7H2O or Na2HPO4.12H2O in 1000 ml
Prepare 100 ml by adding double distilled water
Sr.
No.
pH Na2H2PO4 (ml) Na2HPO4 (ml)
1 6.5 34.25 15.752 7.0 19.75 30.53 7.5 8.0 42.04 8.0 2.65 47.355 8.5 48.25 1.75
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Experimental Design and Observations
6 9.0 44.25 5.757 9.5 39.5 10.58 10.0 31.5 18.5
APPENDIX – IV
Glycine and NaOH Buffer
1) 0.1 M solution of Glycine 7.5 gm of Glycine in 1000 ml of distilled water
2) 0.2 M solution of NaOH 4.0 gm NaOH in 1000 ml of distilled water
clxxxiv
Experimental Design and Observations
APPENDIX - V
LIST OF PUBLICATIONS AND CONFERENCES ATTENDED
PAPERS PUBLISHED
♦ Atri N.S., Upadhyay R.C., Singh Rajinder and Saini M.K. 2007 Effect of culture
media and physical factors on vegetative growth of Lentinus squarrosulus (Mont.)
Singer. Journal Punjab Academy of Sciences 4 (1&2 ) : 91 – 94.
♦ Atri N.S., Upadhyay R.C., Singh Rajinder and Saini M.K. 2009. Effect of carbon
and nitrogen sources on the vegetative growth of Lentinus squarrosulus (Mont.) Singer.
Indian Journal of Mushrooms 27 (1&2):29 – 32.
CONFERENCES ATTENDED
♦ National Symposium on "Researches in Fungal Biology - Emerging Trends" organized by
the Department of Botany, Punjabi University Patiala on January
30 – 31 , 2007.
♦ International Conference on "Mushroom Biology and Biotechnology" at Directorate of
Mushroom Research, Chambaghat, Solan on February 10-11, 2007.
♦ National Conference on “Botanical Researches – Present scenario” organized by
Department of Botany, Punjabi University, Patiala on February 18-19, 2010.
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