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LAMPIRAN

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Lampiran 1 Sekuens DNA Gen FSH sub-unit beta

LOCUS BOVFSHBA 6610 bp DNA linear MAM 27-APR-1993 DEFINITION Bovine follicle stimulating hormone-beta subunit gene, complete cds. ACCESSION M83753 M20185 VERSION M83753.1 GI:163063 KEYWORDS follicle stimulating hormone (FSH); follicle-stimulating hormone beta-subunit. SOURCE Bos taurus (cattle) ORGANISM Bos taurus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia; Pecora; Bovidae; Bovinae; Bos. REFERENCE 1 (bases 1 to 6610) AUTHORS Kim,K.E., Gordon,D.F. and Maurer,R.A. TITLE Nucleotide sequence of the bovine gene for follicle-stimulating hormone beta-subunit JOURNAL DNA 7 (4), 227-233 (1988) PUBMED 2840246 FEATURES Location/Qualifiers source 1..6610 /organism="Bos taurus" /mol_type="genomic DNA" /db_xref="taxon:9913" precursor_RNA 1878..5828 mRNA join(1878..1940,2573..2737,4306..5828) exon 1878..1940 /note="putative" /number=1 intron 1941..2572 /number=1 exon 2573..2737 /number=2 CDS join(2579..2737,4306..4536) /codon_start=1 /product="follicle stimulating hormone beta-subunit" /protein_id="AAA30528.1" /db_xref="GI:163064" /translation="MKSVQFCFLFCCWRAICCRSCELTNITITVEKEECGFCISINTT WCAGYCYTRDLVYRDPARPNIQKTCTFKELVYETVKVPGCAHHADSLYTYPVATECHC SKCDSDSTDCTVRGLGPSYCSFREIKE" sig_peptide 2579..2635 mat_peptide join(2636..2737,4306..4533) /product="follicle stimulating hormone beta-subunit" intron 2738..4305 /number=2 exon 4306..5828 /number=3

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ORIGIN 1547 bp upstream of EcoRI site. 1 cctggcggct acagtccacg gagtcacaaa gagtcggaca cggactgaag caacagcaca 61 cgtgacattc atctcatttt tattcatctc ttgccaatca ctaaaacaca caccagtcag 121 gattttgctc ccctaactcc aaaggaactt ctctagccag gggtattagt gatctttaca 181 actgccacat tattgtgcca tttcccctaa cttaacctgt gagcagtgtc gggtacagtt 241 gtcacccact cctcctgata cacacacttt acttgacttc tgggacacca cattctcttc 301 attcctttct ttcctcaatg acccttctgt ttcctttgga taaaagaaga acaaaacttc 361 atcttatcct tgacctctta gtgctggaat acaccaggct ctacccttgg tccttttaac 421 ttctctattt acatacaccc aatccctcgg taatcttttc aaggcttatg tcaatgactc 481 ccagattttt acctcagaca gtgtatttct ccccaaaccc agtttcagca ttctacatgg 541 atgtcttaaa gatacctcaa gttcaacatg tccacactaa attccttatc tttccaaaaa 601 acttactgta tctctagcca tcccatttca gctgatggca tgtttatcct tcaagtttct 661 caggccataa tccttaaagt catccttggc tcttcctctc acactcaaca ttcatccatc 721 aaaaaattct gtttgctcta ctttcacaat acatccacag ccaaattatg tcttgttcat 781 tgctcccaca ctagtctgaa taaatatcat ttcttaccca ggttgatgca atagcatcta 841 atgggacttc ctgtttctac cttttcaccc ctacattagc aacacggcag tcaaagagat 901 cccttcagac ctaaatcaga tgatgtcact cctctactca aaaccctgca gttggtcccc 961 aatcaacagt aaaaatcaaa gtttatattg gcctaaaatg tcttacatga cttggcttct 1021 gttacatctc tgacatcata tcctaatatt ttcaccattg ttcctttgct agagccacac 1081 tgcttaaata gtaaatattt gttaaacaac tcagtcccag tctccatgca gctcttagtc 1141 tactggggaa acaaagataa aggattaaaa agaagttgat aattaaagct ctcctcttct 1201 tcccattcta aatgctatca aatgaatgtt gaaggttgag gatttgcctg gggtggcagt 1261 actttaggag atgtaggggg taagcaagga catttcaggc aagatgaaca ttgcatatgc 1321 aaagagctag agaaatgaat cagcagaaca tttaaagaat tacaagcaaa gcatcaaaga 1381 ataacatttg aaattagagg atctggatgt aaagaggaat acagtctata ttttagacag 1441 gagtcagatc atgaaagact ttctatttgt tttttccctg ctagttttca aactaccttc 1501 agagtaattt attaaccata tctttttgat acacctcaac agagccaagg caatacttga 1561 aaccaattct gaattcccca agttaaaggt acaaaagaaa aacccaaagt caaatttaat 1621 ttgacaaggt aaaggagtgg gtgttctact atatcaaatt taatctgtac aaaatcatct 1681 ctggtaacat tatttgtccc gatctactgc gtttagacta ctttagtaag gcttgatctc 1741 cctgtctatc caaacactga ttcacttaca gcaagcttca agctaacatt gctcttacta 1801 atatttaaca aatccacaag gtttagttta gtttcacaca attttgtata aaaggtgaac 1861 tgagactaga ctcagctaca gctttcccca gacaaggcag ctgtctacgg aaaagtctca 1921 gcatctacag ttatcaagtg gtgagttact tcaattcgca gatttttaag gacctagaga 1981 acaaatttag agctttttaa attctattgt atagggaatc tgtgcttgtc aacctngctt 2041 cttaaataca ctgtaatcaa tgttgcaagc aattgacttt atatagatca attcactggc 2101 ctctgaaata gtctcatttt aaacaaaggg gggcaaaaac aaatgatatt tatgagatgc 2161 taaaaatgat gaactaattt tgtaatacag aagtttcttt ttcgtctttt aaatggtaat 2221 aaagggacaa atacaatggt catgattgct tgcttcagag taatcactct aaaatagaca 2281 gcagtaatgt tctaaaccaa acactgctct gcttaaatca ttatattgga gttttgatct 2341 gtaaatatgt ttctacttta acaacaacaa caaaaaattg aggagcataa ttagaaatgc 2401 ttgttcaaga aacaagtaaa gcaaaggaaa aggaaggaaa aaacattgct ggagcaaaat 2461 atgataagag acgatggata gaaaaattat ttttggtttg gtcagaatat ataaacgatt 2521 ataattcttt ggaaactcag tttctcacaa gccttaattg tttgtttctc agcccaggat 2581 gaagtctgtc cagttctgtt tccttttctg ttgctggaga gcaatctgct gcagaagctg 2641 cgagctgacc aacatcacca tcacggtgga gaaagaggaa tgtggcttct gcataagcat 2701 caacaccacg tggtgtgcag gctactgcta cacccgggta ggcactttgc tttgctggaa 2761 gtgagggtgc tgagggtctg gacaaggcag gcttcattga ttcccacttg tcaatatttt 2821 aagtttccca acgaatagcc atgagacctt tagccaatac tgttttatga ctggcttttt 2881 taccatgata taaactaaat gtttgggctc agatttgatt acagtaaatg taaagaagag 2941 tcaggtttga cctgaccaat ttagtaatat gctactctat tttaaaaatt tttaattatt 3001 tcaaaaaata gttctatgat agattttaca tatagagaga ttaaaaacat aaacatacat 3061 acatttaata atagaacagc tacaatagtc cccaaaagcc aatttctgta attgaggcct 3121 ttacaacaga gacagtacca aaatatgttt gcaagccaga gaaaagcaca gccacattat 3181 aaaaattgtc ttattggcct actgaatgca aataccaaca agcagcctga gaacgcactc 3241 aaaccgtgta ggactgatat aaccaaaatg aatctgtaaa cccacacagt tctttatact 3301 gttgagaaaa tcaaggcatg gaggcaaaaa acagtttgta ttcacacagc ttctttgaat 3361 taatcccagg ggcagtttct actttctctt ttttccccta taacacctgg ctatctatgc 3421 agaatttcat tcaataagct gaaattgaaa ataccaacct tttaatgaaa tatactttat

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3481 ggggtggtaa atgagtttga ccaaaatcca cttatcacat cttcttggga tatagactta 3541 gtggcatgat attgaaataa cttaaacaga agtaacaact tttccctcag tgcaaatata 3601 tatatatata tatatatata tatatatata tatatttttt ttttttttac tagaaagtgc 3661 tacaaaaaaa cctattctac aaggattaaa gttcttatgt acaaaccatg tagccttaga 3721 aactgtgact tgtgcaaaat atataggctt caaggtggag agatgtctac aagacagcta 3781 aatagttaaa atcttatata agctttcata gtcaggctaa tctaagcatt atcattcccc 3841 attatatact ttttgactct ttataaacac tcagggacaa tgcatttgga aaaaatttct 3901 gaggaattaa acttcactga ttcgtggttc aagttgtcat taatacagct ttcaactaag 3961 taaaaaagtc aaattaaaat ttgtcaattt ctcttgtaat cattttcctc tattgaaatt 4021 caaatttctt gatagtcgaa aaaataatta acattttgac aaagacacaa ggagtaataa 4081 aagaacttat attttctgaa acatctgtag aataatcagg aaacatggta tagcgggtca 4141 tttatgagat atgtttcctt gattaggaat ttcataaaag aattttaaag tgtaaacatt 4201 agagcgagca gtattcaatc cctgtctcac tttgattaag ctaaacagaa acttccagac 4261 tactgtaact catctgtctc tctctctgtc tcctaaacca ctcaggactt ggtatacagg 4321 gacccagcaa ggcccaatat ccagaaaacg tgtaccttca aggagctggt ctacgagacg 4381 gtgaaagtgc ctggctgtgc tcaccatgca gactccctgt acacgtaccc agtagccact 4441 gaatgtcact gcagcaagtg cgacagcgac agcactgact gcaccgtgag aggcctgggg 4501 cccagctact gctccttcag ggaaatcaaa gaataaagag cagcggatgc tttgagctgc 4561 ctacccttat cctaaaggac caaaacatcc aagatgtctg tgtgtacatg tgcgtaggct 4621 gcagaccacc acgggagacc ctactgatct ctgctctcct gagaagggga gctccaggaa 4681 tggagagggc tggggcctgt caccactcag acctgtattc tacatctggt tccataagtc 4741 ttattcactc tcacttaact tacagacacg agggtgcttt cccgtttaat aatcttagaa 4801 atcctctcag gcaatccctt cctctaaaag ctagggatac ggtcccccgg gaaggaaatc 4861 agctaaaagt aggtgaaagt cctaaatgca gcattctcct accagactca agagccctca 4921 agcgcagggc gaacctcttc ttcatgctta tagcctcaaa gcctaacact ccacatagaa 4981 tttagtaaga aactcaataa tggcttcctt aaagaaagta agaccagaaa gttagggatc 5041 ggagaacact gagagaggat gttagaggct atctgatgag gctgcgctcc acagaagcgg 5101 ggcatcagct tcagttctgt ttcgcctctc ctcctctggc acatggagga tggagacagg 5161 ataaaactct ctgaatcatt caggcaggcg gcagttacca agctcaggat ttccaagcta 5221 ttactgccga gtcctttagt taagggcaaa agcaagaaat gttaatatag acttatagaa 5281 actagccgcg tctgttccat catttaaata aatggaacaa atgctgtcag ggtcctgcaa 5341 aactcccttt ggaaagtaca gagaactata tcaccctgcc cttgaatgta gggtctactc 5401 ctttggaaag aggtggattt gagagcaggt ttaaaagcaa ttttgacaac ttaataagtg 5461 catttatctt atcttcaaat acatttatgt aaagaaaatg ctcttttaga aagattagcc 5521 ataacaaaaa aaaactcact gctgttttct aaagttctct ctttgtcagt actctttttt 5581 gtctatccat gttctcccag acctgtttct ttcaagagga taaatattga gactatttca 5641 tgagttgatt tcgagatgtt acctctcagt tatgtacttg tttcatactc atattaactg 5701 gtttatttag gtcctatttt tttaaataaa gatgctagcc accagagtca taggtttgga 5761 tttttttcac atgtaagcag atgactaaaa tgtctgtatg tttatgatgg taataaaata 5821 aatcttacaa aaaaatccct agtgcttcta caatatttac ttgtactgac ctttggaaat 5881 attaatgttg ttacatttct aaaagtattt cctcgtttaa attttgggtc actttttgaa 5941 ctctttttct gagacagtgg gggtgggggg aaaccaaagg gagaacacag agtggatggg 6001 gacgctggaa agtccaagaa agctcattct ttggattttt gagatgaaaa attgaaggcc 6061 aaggatgtca agtgacccaa cttaggtcac aaagactgta tatgcaggca ggataagaac 6121 cctggatccc gatctcttag cccaactctc ctatgataat ctgtcatatt atgtcatgtt 6181 ggcaaggact gatgtaaaat tgcaggtact ggtaatcagc tatgtgcatt ccaatctggt 6241 actgagtgtg ccttatagct atattctcta caatattttg acaggttgaa attagggcta 6301 caaggtctaa taagatgctt taaattataa cctctgttcc ctcagtggcc tcttatcagc 6361 aaagaacagc ctaatgctgt tttcttgaat aagggagtag tgaaaaatca attgaatctg 6421 gaaggggaag ttgaacatgg aaggcacatt gactgctaca tctcaaataa atatttatct 6481 aaagtcagac ccaacaattt taatgacatt gaaaagctct aagctgttgg cctgtgtttg 6541 ccaaaagaaa tatatatata tgtgtgggtg tgggtgtgtg tgtgtatgtg tatatatata 6601 tatatatata

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Lampiran 2 Sekuens Gen FSH Reseptor

LOCUS BOVFSHR 2375 bp mRNA linear MAM 13-OCT-1994 DEFINITION Bovine follicle stimulating hormone receptor (FSHR) mRNA, complete cds. ACCESSION L22319 VERSION L22319.1 GI:404671 KEYWORDS follicle stimulating hormone (FSH) receptor. SOURCE Bos taurus (cattle) ORGANISM Bos taurus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia; Pecora; Bovidae; Bovinae; Bos. REFERENCE 1 (bases 1 to 2375) AUTHORS Houde,A., Lambert,A., Saumande,J., Silversides,D.W. and Lussier,J.G. TITLE Structure of the bovine follicle-stimulating hormone receptor complementary DNA and expression in bovine tissues JOURNAL Mol. Reprod. Dev. 39 (2), 127-135 (1994) PUBMED 7826612 REFERENCE 2 (bases 1 to 2375) AUTHORS Lussier,J.G. TITLE Direct Submission JOURNAL Submitted (30-SEP-1993) Jacques G. Lussier, CRRA, Faculty of Veterinary Medicine, University of Montreal, P.O. Box 5,000, 3,200 Sicotte, St-Hyacinthe, Quebec, J2S 7C6, Canada COMMENT Original source text: Bos taurus (strain Holstein) cDNA to mRNA. FEATURES Location/Qualifiers source 1..2375 /organism="Bos taurus" /mol_type="mRNA" /strain="Holstein" /db_xref="taxon:9913" /tissue_type="ovaries/testes" gene 1..2375 /gene="FSHR" 5'UTR 1..64 /gene="FSHR" CDS 65..2152 /gene="FSHR" /codon_start=1 /product="follicle stimulating hormone receptor" /protein_id="AAC37324.1" /db_xref="GI:404672" /translation="MALLLVALLAFLSLGSGCHHRLCHCSNGVFLCQESKVTEIPSDL PRDAVELRFVLTKLRVIPKGAFSGFGDLEKIEISQNDVLEVIEANVFSNLPKLHEIRI

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EKANNLLYIDPDAFQNLPNLRYLLISNTGIKHLPAVHKIQSLQKVLLDIQDNINIHTV ERNSFMGLSFESMTVWLSKNGIQEIHNCAFNGTQLDELNLSDNSNLEELPNDVFQGAS GPVILDISRTRIRSLPSYGLENLKKLRAKSTYRLKKLPSLEKFVTLVEASLTYPSHCC AFANWRRQTSDLHPICNKSILRQEVDDMTQARGQRVSLAEDDEPSYAKGFDVMYSEFD YDLCNEVVDVTCSPEPDAFNPCEDIMGDDILRVLIWFISILAITGNILVLVILITSQY KLTVPRFLMCNLAFADLCIGIYLLLIASVDVHTKTEYHNYAIDWQTGAGCDAAGFFTV FASELSVYTLTAITLERWHTITHAMQLECKVQLRHAASIMLVGWIFAFAVALFPIFGI SSYMKVSICLPMDIDSPLSQLYVMSLLVLNVLAFVVICGCYTHIYLTVRNPNITSSSS DTKIAKRMAMLIFTDFLCMAPISFFAISASLKVPLITVSKSKILLVLFYPINSCANPF LYAIFTKNFRRDFFILLSKFGCYEVQAQTYRSETSSTAHNFHPRNGHCPPAPRVTNGS NYTLIPLRHLAKN" sig_peptide 65..118 /gene="FSHR" mat_peptide 119..2149 /gene="FSHR" /product="follicle stimulating hormone receptor" 3'UTR 2150..2375 /gene="FSHR" ORIGIN 1 tctgaaatct gtgcaggatt ttctctgcag aggcagaaga aagcaggtgg atggataagt 61 aaacatggcc ttgctcctgg tggccttgct cgcattcctg agcctgggct caggatgcca 121 tcatcgactc tgtcactgct ctaacggagt tttcctctgc caggagagca aggtgacaga 181 gattccctcc gacctcccca gggatgcagt cgaactgagg tttgttctca ccaagcttcg 241 agtcatccca aaaggagcct tttcaggatt tggagacctg gagaaaatag agatctcaca 301 gaatgatgtc ttggaagtga tagaggcaaa cgtgttctcc aacctgccca aactacatga 361 aattagaatt gaaaaggcca acaacctgct atacatcgac cctgatgcct tccagaacct 421 tcccaacctc cgatatctgt taatatccaa cacaggtatt aagcacttgc cagctgttca 481 caagattcag tctctccaaa aggttttact agatattcaa gataatataa acatccacac 541 agttgaaaga aattctttca tggggctaag ttttgaaagt atgactgtat ggctgagtaa 601 gaatgggatt caagaaatac acaactgtgc attcaatgga acccaactcg atgagctgaa 661 tctaagtgat aacagtaatt tggaagaact gcctaatgat gttttccagg gagcctctgg 721 accggtcatt ctagatattt caagaaccag gatccgttcc ctgcctagct atggcttaga 781 aaatcttaag aagctgcggg ccaagtcaac ttaccgctta aaaaagcttc ccagtctgga 841 aaaatttgtc acactcgtgg aggccagcct cacctacccc agccactgct gtgcctttgc 901 aaactggagg cggcaaacct ctgaccttca tccaatttgc aacaaatcta ttttaaggca 961 agaagttgat gacatgactc aggctagggg tcagagagtc tctttggcag aagatgatga 1021 gcccagctat gccaaaggat ttgacgtgat gtacagtgaa tttgactatg acttatgcaa 1081 tgaagtggtt gatgtgactt gctcccctga gccagacgca tttaatccat gtgaagatat 1141 catgggggat gatattctca gagtcttgat atggtttatt agcatcctgg ccatcactgg 1201 gaacatccta gtgctggtga tcctgatcac cagccagtat aaactcacag tcccccggtt 1261 cctcatgtgc aatctggcct ttgccgatct ctgcattgga atctacctgc tgctcatagc 1321 ctcagttgat gtccacacca aaacggagta ccacaactat gccatcgact ggcaaactgg 1381 agcaggctgt gatgctgccg gctttttcac tgtctttgcc agtgagctct cagtctacac 1441 tctgaccgcc atcacgctgg aaagatggca taccatcacc catgccatgc agctcgaatg 1501 caaagtgcag ctccgccatg ctgccagcat catgctggtg ggctggatct ttgcttttgc 1561 agttgccctt tttcccatct ttggcatcag cagctacatg aaggtgagca tctgcctgcc 1621 catggacatt gacagcccct tgtcacaact ctatgtcatg tccctccttg tgctcaatgt 1681 cctggccttt gtggtcatct gtggctgcta cactcacatc tacctcacgg tgaggaaccc

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1741 caacatcaca tcctcttcta gtgacaccaa gatagccaag cgcatggcca tgctcatctt 1801 caccgacttc ctctgcatgg cacccatctc cttctttgcc atctctgcct ccctcaaggt 1861 gcccctcatc actgtgtcca agtcaaagat cctcctggtc ctgttctacc ccatcaactc 1921 ctgtgccaac cccttcctct atgccatctt caccaagaac ttccgcaggg atttcttcat 1981 tctgctgagc aagtttggct gctatgaagt gcaagcccag acctataggt cagaaacctc 2041 atccactgcc cacaactttc atccaaggaa cggccactgc cccccagctc ccagggttac 2101 taatggttcc aattacacac ttatccccct aagacattta gccaagaact aaaacacaat 2161 gtacaaatgt ttctgagtgt tgaatgacag ataagtcttg cctttgaaga atatgccatg 2221 gaagagctga cagagcactt ccacttactt catctaattt aatctccctg gcacatccat 2281 aaggtaaatt ggtcagaaac tattaacttc acatgattca ttaggaaact gaagacttca 2341 taacaacatt aataattaaa ataatgtagt actac

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Lampiran 3 Sekuens DNA Gen GH

LOCUS BOVGHGH 2856 bp DNA linear MAM 27-APR-1993 DEFINITION Bovine growth hormone gene, complete cds. ACCESSION M57764 M28453 VERSION M57764.1 GI:163091 KEYWORDS growth hormone. SOURCE Bos taurus (cattle) ORGANISM Bos taurus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia; Pecora; Bovidae; Bovinae; Bos. REFERENCE 1 (bases 1 to 2856) AUTHORS Gordon,D.F., Quick,D.P., Erwin,C.R., Donelson,J.E. and Maurer,R.A. TITLE Nucleotide sequence of the bovine growth hormone chromosomal gene JOURNAL Mol. Cell. Endocrinol. 33 (1), 81-95 (1983) PUBMED 6357899 COMMENT Original source text: Bovine liver DNA. FEATURES Location/Qualifiers source 1..2856 /organism="Bos taurus" /mol_type="genomic DNA" /db_xref="taxon:9913" /tissue_type="liver" gene join(649..723,971..1131,1359..1475,1703..1864,2138..2439) /gene="GH1" mRNA join(649..723,971..1131,1359..1475,1703..1864,2138..2439) /gene="GH1" /product="growth hormone" exon 649..723 /gene="GH1" /number=1 CDS join(711..723,971..1131,1359..1475,1703..1864,2138..2338) /gene="GH1" /codon_start=1 /product="growth hormone" /protein_id="AAA30544.1" /db_xref="GI:163092" /translation="MMAAGPRTSLLLAFALLCLPWTQVVGAFPAMSLSGLFANAVLRA QHLHQLAADTFKEFERTYIPEGQRYSIQNTQVAFCFSETIPAPTGKNEAQQKSDLELL RISLLLIQSWLGPLQFLSRVFTNSLVFGTSDRVYEKLKDLEEGILALMRELEDGTPRA GQILKQTYDKFDTNMRSDDALLKNYGLLSCFRKDLHKTETYLRVMKCRRFGEASCAF" intron 724..970 /gene="GH1" /number=1 exon 971..1131 /gene="GH1"

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/number=2 intron 1132..1358 /gene="GH1" /number=2 exon 1359..1475 /gene="GH1" /number=3 intron 1476..1702 /gene="GH1" /number=3 exon 1703..1864 /gene="GH1" /number=4 intron 1865..2137 /gene="GH1" /number=4 exon 2138..2439 /gene="GH1" /number=5 ORIGIN 1 gtactggggt gggttgcctt tctcttctcc aggggattta tctgacccag ggattgaacc 61 tgagtctcct gcatttgcag ctagattctt tacggctgag ccacctggga agcccattcg 121 cttctgctgc tgctgctgct gctaagttgc ttcagtcgtg tccgacctgt gcgacgccat 181 agacagcagc ccaccaggtc cccgtccctg ggattctcca ggcaagaaca ttggagtggg 241 ttgccatttc ctcctccaat gcatgaaagt gaaaagtgaa agtgaagtca ctcagttgtg 301 tccgaccctc agcgacccca tggactgcag ccttccagaa tggggtgcca ttgccttctc 361 ctcgcttctg ctacctcccc tttaaaaaga aaacctatgg ggtgggctct caagctgaga 421 ccctgtgtgc acagccctct ggctggtggc agtggagacg ggatgatgac aagcctgggg 481 gacatgaccc cagagaagga acgggaacag gatgagtgag aggaggttct aaattatcca 541 ttagcacagg ctgccagtgg tccttgcata aatgtataga gcacacaggt ggggggaaag 601 ggagagagag aagaagccag ggtataaaaa tggcccagca gggaccaatt ccaggatccc 661 aggacccagt tcaccagacg actcagggtc ctgtggacag ctcaccagct atgatggctg 721 caggtaagct cgctaaaatc ccctccattc gcgtgtccta aaggggtaat gcggggggcc 781 ctgccgatgg atgtgttcag agctttgggc tttagggctt ccgaatgtga acataggtat 841 ctacacccag acatttggcc aagtttgaaa tgttctcagt ccctggaggg aagggtaggt 901 ggggctggca ggagatcagg cgtctagctc cctggggccc tccgtcgcgg ccctcctggt 961 ctctccctag gcccccggac ctccctgctc ctggctttcg ccctgctctg cctgccctgg 1021 actcaggtgg tgggcgcctt cccagccatg tccttgtccg gcctgtttgc caacgctgtg 1081 ctccgggctc agcacctgca tcagctggct gctgacacct tcaaagagtt tgtaagctcc 1141 cgagggatgc gtcctagggg tggggaggca ggaaggggtg aatccacacc ccctccacac 1201 agtgggagga aactgaggag ttcagccgta ttttatccaa gtagggatgt ggttagggga 1261 gcagaaacgg gggtgtgtgg ggtggggagg gttccgaata aggcggggag gggaaccgcg 1321 caccagctta gacctgggtg ggtgtgttct tcccccagga gcgcacctac atcccggagg 1381 gacagagata ctccatccag aacacccagg ttgccttctg cttctctgaa accatcccgg 1441 cccccacggg caagaatgag gcccagcaga aatcagtgag tggcaacctc ggaccgagga 1501 gcaggggacc tccttcatcc taagtaggct gccccagctc ccgcaccggc ctggggcggc 1561 cttctccccg aggtggcgga ggttgttgga tggcagtgga ggatgatggt gggcggtggt 1621 ggcaggaggt cctcgggcag aggccgacct tgcagggctg ccccagaccc gcggcaccca 1681 ccgaccaccc acctgccagc aggacttgga gctgcttcgc atctcactgc tcctcatcca 1741 gtcgtggctt gggcccctgc agttcctcag cagagtcttc accaacagct tggtgtttgg 1801 cacctcggac cgtgtctatg agaagctgaa ggacctggag gaaggcatcc tggccctgat 1861 gcgggtgggg atggcgttgt gggtcccttc catgtggggg ccatgcccgc cctctcctgg 1921 cttagccagg agaatgcacg tgggcttggg gagacagatc cctgctctct ccctctttct 1981 agcagtccag ccttgaccca ggggaaacct tttccccttt tgaaacctcc ttcctcgccc 2041 ttctccaagc ctgtagggga gggtggaaaa tggagcgggc aggagggagc tgctcctgag 2101 ggcccttcgg cctctctgtc tctccctccc ttggcaggag ctggaagatg gcaccccccg 2161 ggctgggcag atcctcaagc agacctatga caaatttgac acaaacatgc gcagtgacga 2221 cgcgctgctc aagaactacg gtctgctctc ctgcttccgg aaggacctgc ataagacgga 2281 gacgtacctg agggtcatga agtgccgccg cttcggggag gccagctgtg ccttctagtt

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2341 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc 2401 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt 2461 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca 2521 ggcatgctgg ggatgcggtg ggctctatgg gtacccaggt gctgaagaat tgacccggtt 2581 cctcctgggc cagaaagaag caggcacatc cccttctctg tgacacaccc tgtccacgcc 2641 cctggttctt agttccagcc ccactcatag gacactcata gctcaggagg gctccgcctt 2701 caatcccacc cgctaaagta cttggagcgg tctctccctc cctcatcagc ccaccaaacc 2761 aaacctagcc tccaagagtg ggaagaaatt aaagcaagat aggctattaa gtgcagaggg 2821 agagaaaatg cctccaacat gtgaggaagt aatgag

Lampiran 4 Hasil Analisa Data Gen FSH Sub-unit Beta PCR-RFLP

Breed

Genotipe

Σ

Freq Genotipe Freq Alel

AA AB BB AA AB BB A B

Bali 0 0 225 225 0.000 0.000 1.000 0.000 1.000

Brahman 6 12 51 69 0.087 0.174 0.739 0.174 0.826

FH 81 2 9 92 0.880 0.022 0.098 0.891 0.109

Simmental 24 8 8 40 0.600 0.200 0.200 0.700 0.300

Limousin 29 14 1 44 0.659 0.318 0.023 0.818 0.182

Breed

Nilai Harapan H0 He X2 PIC

AA AB BB

Bali 0.000 0.000 225 0.000 0.000 - -

Brahman 2.087 47.087 47.087 0.174 0.287 10.751* 0.246

FH 73.087 1.087 1.087 0.022 0.194 72.514* 0.175

Simmental 19.600 3.600 3.600 0.200 0.420 10.975* 0.332

Limousin 29.455 1.455 1.455 0.318 0.298 0.212tn 0.253

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Lampiran 5 Hasil Analisa Data Gen FSH Sub-unit Beta PCR-SSCP

Breed

Genotipe

Σ

Freq Genotipe Freq Alel

AA AB BB AA AB BB A B

Bali 0 0 225 225 0.000 0.000 1.000 0.000 1.000

Brahman 20 15 39 74 0.089 0.067 0.173 0.372 0.628

FH 44 19 20 83 0.196 0.084 0.089 0.645 0.355

Simmental 10 16 17 43 0.044 0.071 0.076 0.419 0.581

Limousin 14 13 17 44 0.062 0.058 0.076 0.466 0.534

Breed

Nilai Harapan

H0 He X2 PIC

AA AB BB

Bali 0.000 0.000 225.000 0.000 0.000 - 0.000

Brahman 10.220 34.561 29.220 0.203 0.467 23.705 0.841

FH 34.485 38.561 10.485 0.229 0.458 20.783 0.469

Simmental 7.535 20.930 14.535 0.372 0.487 2.386 0.424

Limousin 9.551 21.551 12.551 0.295 0.498 7.265 0.509

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Lampiran 6 Hasil Analisa Data Gen FSH Reseptor PCR-RFLP

Breed

Genotipe

Σ

Freq Genotipe Freq Alel

AA AB BB AA AB BB A B

Bali 7 0 176 183 0.038 0.000 0.962 0.038 0.962

Brahman 23 26 24 73 0.126 0.142 0.131 0.493 0.507

FH 81 2 9 92 0.443 0.011 0.049 0.891 0.109

Simmental 17 10 17 44 0.093 0.055 0.093 0.500 0.500

Limousin 28 10 10 48 0.153 0.055 0.055 0.688 0.313

Breed

Nilai Harapan

H0 He X2 PIC

AA AB BB

Bali 0.268 13.464 169.268 0.000 0.074 - 0.000

Brahman 17.753 36.493 18.753 0.356 0.500 6.035 0.337

FH 73.753 17.826 1.087 0.022 0.194 72.514 0.462

Simmental 11.000 22.000 11.000 0.227 0.500 13.091 0.287

Limousin 22.688 20.625 4.688 0.208 0.430 12.738 0.352

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Lampiran 7 Hasil Analisa Data Gen GH PCR-RFLP

Breed

Genotipe

Σ

Freq Genotipe Freq Alel

AA AB BB AA AB BB A B

Bali 0 0 225 225 0.000 0.000 1.000 0.000 1.000

Brahman 41 21 7 69 0.182 0.093 0.031 0.746 0.254

FH 22 30 40 69 0.098 0.133 0.178 0.402 0.598

Simmental 20 20 8 48 0.089 0.089 0.089 0.625 0.375

Limousin 12 16 4 32 0.053 0.071 0.071 0.625 0.375

Breed

Nilai Harapan

H0 He X2 PIC

AA AB BB

Bali 0.000 0.000 225.000 0.000 0.000 - 0.000

Brahman 38.438 26.123 4.438 0.304 0.379 2.654 0.246

FH 14.880 44.239 32.880 0.326 0.481 9.531 0.175

Simmental 18.750 22.500 6.750 0.417 0.469 0.593 0.593

Limousin 12.500 15.000 4.500 0.500 0.469 0.142 0.142

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Lampiran 8 Foto abnormalitas sperma

Abnormalitas tipe abaxial

Abnormalitas tipe Knobbed acrosome defect

ン ´ﾏ

ン ´ﾏ

92

1 : Abnormalitas tipe Macrocephalus

2 : Abnormalitas tipe Knobbed acrosome defect

Abnormalitas tipe Microcephalus

1

2

ン ´ﾏ

ン ´ﾏ

93

Abnormalitas tipe Round head

Abnormalitas tipe Round head

ン ´ﾏ

ン ´ﾏ

94

Abnormalitas tipe Round head

Abnormalitas tipe Knobbed acrosome defect

ン ´ﾏ

3 ´ﾏ

95

Lampiran 9 Foto sapi Bali

Sumber : Dokumentasi pribadi

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