1 Cover LT 2004 - Official Portal of UKM Report... · 2014-12-04 · Professor Dr. Ramelah Mohamed Suhaili Abu Bakar Principal Fellow VK7 Scientific Officer C41 (IRPA) [email protected]

CONTENTS

Message from Vice Chancellor UKM 1 Message from the Director 2 Directory 3 Introduction 4 Organisation chart 5 Research programme at UMBI 6 HUKM-UMBI Genomics Unit 7 Profile of Fellows 8 Current infrastructure and key systems 15 Events in 2004 16 Other activities of researchers 19 Post graduate research students in 2004 21 Publications 23 Awards won in 2004 by members of UMBI 30 Networking & collaboration 32

VISION

To be the leading institution in molecular medicine by the year 2006 and to achieve world-class status by 2013

MISSION Be competitive and achieve excellence in molecular medicine

by utilizing all available and expertise, by adopting and developing latest technologies, and by conducting

cutting edge world class research

UKM MEDICAL MOLECULAR BIOLOGY INSTITUTE (UMBI)

Annual Report UMBI 2004

1

MESSAGE FROM VICE CHANCELLOR UKM Professor Dato’ Dr. Mohd Salleh Mohd Yasin

Vice-Chancellor Universiti Kebangsaan Malaysia Drs (ITB), PhD (Bath)

It gives me great pleasure to witness the progress

and and development of the UKM Medical Molecular

Biology Institute (UMBI). I am certainly proud of what

UMBI has achieved in the year 2004 as presented in

this annual report and is certainly on track to become

the leading national institution in molecular medicine

in the future years. I am certainly pleased to see that

UMBI has grown in terms of the number of fellows as

well as the amount of research grants acquired. The

fact that UMBI has successfully brought together 6

research fellows to the institute within a short period

of time is already an achievement. UKM celebrates it 35th anniversary this year and

certainly has consolidated its position as the

national university as well as a leading institution in

the country. Research as always been an important

agenda for UKM and this is evident in the numerous

successes of our researcher acquiring grants,

publishing in high impact factor journals UKM

celebrates it 35th anniversary this year and certainly

has consolidated its position as the national university

as well as a leading institution in the country.

Research as always been an important agenda for

UKM and this is evident in the numerous successes

of our researchers in acquiring grants, publishing in

high impact factor journals as well as winning medals

at expositions and exhibitions at both local and

international levels. UKM currently has 7 research

institutes, each focusing on a niche area based on the

strength and resources available. We are certain that

UKM would soon achieve the status of a research-

intensive university in the near future.

The management will continue to provide the

necessary support especially in terms of infrastructure

as well as personnel. In return, we would like to see

more lecturers involved in research, more

postgraduate students trained in various fields, more

publications in peer-reviewed journals and products

which are commercialisable.


Annual Report UMBI 2004 2

MESSAGE FROM THE DIRECTOR

Professor Dr. A Rahman A Jamal

Director

UKM Medical Molecular Biology Institute

MD (UKM), MRCP (Ireland), PhD (London)

The UKM Medical Molecular Biology Institute (UMBI)

was launched in July 2003 and is already making its

mark as a leading research center in molecular

medicine locally. The researchers at UMBI are

focusing on key research areas in tandem with

advances in the scientific world. Research grants

continue to be sought for either at the local or

international level. Our laboratories are well equipped

with the latest automated systems in genomics and

gene expression analysis. Our smart-partnership with

the industry is highlighted by the acquisition of the

Affymetrix microarray system from Research

Instruments. UMBI would soon be the referral center

for gene expression analysis in Malaysia. UMBI has

also set up a genomics unit with the Hospital UKM

mainly focusing on molecular diagnostics. We would

soon be offering genetic screening tests including

oncogenes, tumour suppressor genes as well as

conditions such as Fragile X, congenital myopathies

as well as inborn errors of metabolism. We have also

initiated our own BIOBANK, with the assistance of

HUKM, which would systematically store

biospecimens including tumour tissues and blood

specimens, with prior informed consent taken from all

patients. The BIOBANK which has been approved by

the institution’s ethics committee, is the first

comprehensive model of a biobank in the country.

Researchers at UMBI collaborate not only with

researchers from the Faculty of Medicine but also

from other faculties and other local institutions. UMBI

also practices an open-door policy aimed at

strengthening ties and establishing new

collaborations. UMBI is also undertaking a research

collaboration with the King’s College School of

Medicine in London UK. Our researchers will be

spearheading a top-down project on stem cell

involving 4 other institutions in the country. UMBI

would focus on gene therapy as well as differentiation

of stem cells into specific tissues. UMBI also trains

graduate and post-graduate students in the field of

molecular medicine to help build the capacity of

researchers in this field.

As the nation moves forward with the National

Biotechnology Programme, we certainly hope that

UMBI will play a major role in the component of

Medical Biotechnology. The future looks very bright

for UMBI.


Annual Report UMBI 2004

3

DIRECTORY

Professor Dr. A Rahman A Jamal Bahiyah Tuah Director VK7 Scientific Officer C41 (Administration)

[email protected] [email protected] ext: 2189, 2454 ext: 3771 Professor Dr. Wan Zurinah Wan Ngah Roszita Ibrahim Principal Fellow VK7 Scientific Officer C41 (HUKM) [email protected] [email protected] 03-4040 5222 ext: 2057 Professor Dr. Ramelah Mohamed Suhaili Abu Bakar Principal Fellow VK7 Scientific Officer C41 (IRPA) [email protected] [email protected] ext: 2209 ext: 2057 (Appointment ended on 1 July 2005)

Associate Professor Dr. Roslan Harun Rosniza Mohamed Hussain Senior Fellow DU54 Medical Laboratory Technologist U29 [email protected] [email protected] ext: 2396 ext: 2454 Associate Professor Dr. Ruszymah Hj. Idrus Narimah Khaliani Senior Fellow DU54 Administrative Assistant (Secretarialship) N17 [email protected] [email protected]

ext: 2449 / 4040 5424 ext: 2454 (Appointment ended on 18 January 2005) Associate Professor Dr. Mohd Zaki Salleh Nurul Izza Umar Senior Fellow DS53 Administrative Assistant (P & O) N17 [email protected] [email protected] ext: 2032 ext: 2454 Associate Professor Dr. Syed Zulkifli Syed Zakaria Zuraini Abd. Razak Senior Fellow DU54 Laboratory Assistant C17 [email protected] [email protected] ext: 2198 ext: 2454 Nor Azian Abd. Murad Suriyenty Abdullah Tutor DA41 Laboratory Assistant C17 [email protected] ext: 2454 ext: 2030 (Appointment ended on 25 April 2005) Siti Azma Yusof Hazli Hapiz Tutor DA41 Junior Administrative Assistant N1 [email protected] [email protected] ext: 2031 ext: 2454



INTRODUCTION It has been an eventful year for UMBI in 2004. We started off the year by appointing 2 new principal fellows. Professor Dr. Wan Zurinah Wan Ngah, a senior professor and researcher from the Department of Biochemistry, Faculty of Medicine joined us as a principal fellow in January bringing with her the expertise in the field of antioxidants and metabolism. Shortly after, Professor Ramelah Mohamed, a senior professor in microbiology and a leading authority in Helicobacter pylori in the region, was appointed as a principal fellow of UMBI. Later in the year two more fellows joined the team. Associate Professor Dr. Mohd Zaki Salleh, formerly the Deputy Director of the Institute for Research in Molecular Medicine (INFORMM) USM, joined the Faculty of Medicine and was seconded to UMBI. Prof. Zaki’s expertise is in microbial genomics as well as molecular diagnostics of enteric pathogens. In the second half of 2004, we were joined by Associate Professor Dr. Syed Zulkifli Syed Zakaria who was appointed as a senior research fellow. Prof. Syed’s research forte is in molecular epidemiology as well as the gene-environment interaction in diseases. The addition of the four new research fellows has certainly consolidated UMBI’s strength in terms of research capabilities as well as expertise. UMBI has a total of RM11.7 million in research grants from sources like IRPA as well as under the National Biotechnology Directorate. UKM also allocated RM150,000 for short-term grants in fundamental research for UMBI fellows. In fact, two projects have been approved under this scheme: one project looking at molecular mechanisms of glucocorticoid resistance in leukaemias and another project looking at single nucleotide polymorphisms in diabetes mellitus. Although each fellow leads his/her own group of researchers, there are various platforms where each group can interact. Each research fellow updates the others during monthly management meetings plus other informal discussions. UMBI also has regular brainstorming sessions to put forward ideas for new research projects. In fact, this session is open to members from the faculty as well to facilitate research in molecular medicine. The relations of UMBI with the Faculty of Medicine and Hospital UKM have flourished over the past year and the fact that the dean and the directors of the HUKM and UMBI sits together at a joint management committee to discuss common issues as well as helping each other to consolidate and achieve excellence for UKM. In a nutshell, UMBI should be treated like a central laboratory for research in molecular medicine for the faculty and for molecular diagnostics for the hospital. In fact, the equipments at UMBI are open to be used by all members of the faculty and hospital. In addition, the HUKM-UMBI Genomics Unit was formalized to facilitate molecular diagnostics as well as the implementation of the HUKM-UMBI Biobank. The molecular diagnostic services will focus on cancer predisposition genes, rare genetic diseases and inborn error of metabolism, as well as genetic tests, which are not provided elsewhere in the country. UMBI had an eventful year in terms of continuing medical education programmes as well as providing training in molecular medicine. Our monthly molecular research updates are very much well received and are even attended by clinicians as well as participants outside UKM. UMBI also organized several workshops including the SPSS workshop as well as the microarray technique. Our research fellows have also published articles in peer-reviewed journals as well as numerous abstracts and posters. We have also won awards at local as well as international conventions and conferences. This annual report serves as documentation on UMBI at both the organisational and operational levels as well as the people that make UMBI what it is today. Everyone, including the research fellows, the scientific officers, the technologists, the laboratory assistants and others inside and outside UMBI has contributed significantly to UMBI’s successes in the year 2004. Entrance to UMBI Monthly management meeting UMBI’s temporary office



ORGANISATION CHART

Vice Chancellor

Administration Office and Support Staff Administrative Officer Scientific Officers Medical Technologists Administrative Assistants Laboratory Assistants

Director

Council of Scientific

Advisers

Assoc. Prof. Dr. Roslan Harun

Molecular Basis of

Disease

Prof. A Rahman A Jamal

Cancer Research Stem cell research

Prof. Dr. Wan Zurinah Wan Ngah

Metabolism and Anti-

Oxidants

Assoc. Prof. Dr. Zaki Salleh

Infectious diseases Pharmacogenomics

Assoc. Prof. Dr. Syed Zulkifli Syed Zakaria

Molecular Epidemiology

Prof. Dr. Ramelah Mohamed

Infectious diseases

Assoc. Prof. Dr. Ruszymah Idrus

Tissue Engineering



RESEARCH PROGRAMME AT UMBI Our research in molecular medicine includes fundamental, applied and experimental/development research. There are

seven thrust areas of research activities at UMBI, based on the strength and expertise of the research fellows and that

available at the faculty. Niche areas which are operational Themes Researchers Position Cancer Research Professor Dr. A Rahman A Jamal Director and Principal Fellow Molecular basis of disease Assoc. Prof. Dr. Roslan Harun Senior Fellow Tissue Engineering Assoc. Prof. Dr. Ruszymah Hj. Idrus Senior Fellow Metabolisme and antioxidants Professor Dr. Wan Zurinah Wan Ngah Principal Fellow Stem cell and gene therapy Professor Dr. A Rahman A Jamal Director and Principal Fellow Molecular epidemiology Assoc. Prof. Dr. Syed Zulkifli Syed Zakaria Senior Fellow Infectious Diseases Professor Dr. Ramelah Mohamed Principal Fellow Assoc. Prof. Dr. Mohd Zaki Salleh Senior Fellow

Niche areas which are being set up Themes Pharmacogenomics



HUKM-UMBI GENOMICS UNIT The HUKM-UMBI Genomics Unit was set up to enhance collaboration between the hospital and UMBI. This smart

partnership allows UMBI to tap into the vast resources available at HUKM as well as providing facilities which would not

only consolidate diagnostic services but also research activities amongst members of the medical complex. The unit

manages the HUKM-UMBI Biobank and the Molecular Diagnostic Services and receives funding from HUKM.

BioBank HUKM-UMBI The BioBank HUKM-UMBI was initiated by UMBI with the collaboration of Hospital UKM, as a visionary initiative to

complement research in molecular medicine. The main objective of the BioBank is to systematically collect and archive

clinical specimens (including cancer tissue samples, blood samples, etc) from patients undergoing surgeries and

diagnostic procedures. This would provide an invaluable resource for researchers in this field. Informed consent will be

taken from all patients upfront especially pertaining to the permission to store excess tissues removed during surgery and

for the specimens to be used for teaching and research. The implementation of the BioBank has been approved by the

institution Research and Ethics Committee at the Faculty of Medicine UKM. The BioBank will potentially become a

valuable resource centre for researchers within and outside UKM and a ideal model for a National BioBank in future.

Molecular Diagnostic Services UMBI aims to be one of the leading centres for molecular diagnostics in Malaysia. Although genetic testing for some

diseases like thalassaemia and G6PD deficiency are available at certain centres, many other tests are not available

locally. Clinicians send samples overseas and patients have to pay a hefty sum for these diagnostic services. UMBI

would soon be offering an array of diagnostic tests under its Molecular Diagnostic Services. We would initially focus on

important genetic diseases where the molecular diagnostic tests are not offered elsewhere in the country. Amongst the

tests being optimised are as follows:

BRCA1 and BRCA2 genes for breast cancer

P53, K-ras, APC genes

RB1 gene for retinoblastoma

LDL receptor gene for familial hypercholesterolaemia (FH)

Fragile-X Syndrome

Thalassaemia

Huntington Disease



PROFILE OF FELLOWS Professor Dr. A Rahman A Jamal Qualifications: MD (UKM), MRCP (Ireland), PhD (London) PhD thesis in Haematology (awarded by University of London in 1996) Research interests

Molecular biology of cancers Our research mainly focuses on the role of oncogenes, tumour suppressor genes, DNA repair genes in several cancers including colorectal cancer, bladder cancer and breast cancer. We also have a strong childhood leukaemia study group looking at gene arrangements, multi-drug resistance pathways as well as minimal residual disease.

Thalassaemia Our team focuses on morbidity and complications as well as the epidemiological aspects of the disease.

Clinical oncology Our team focuses on survival studies, clinical trials for febrile neutropenia and late effects of therapy.

Selected publications

Rahman Jamal, Baizura Jamaluddin, Idris Nor, Hamidah Alias, Jeffrey Abu Hassan, Roslan Harun. Abnormalities in lung function among multiply transfused thalassaemia patients: Results from a thalassaemia daycare centre in Malaysia. South East Asian Journal of Tropical Medicine and Public Health, 2005;36;1-5.

Rahman Jamal,Fazlina N, Maha A, Zarina AL, Hamidah A, Zulkifli SZ, Cheong SK, Ainoon O, Hamidah NH..

Multidrug resistance proteins in acute leukaemia cases in a typical hematology-oncology unit in Malaysia. Pediatric Blood and Cancer, 2004;43:S1-394.

Balraj P, Concannon P, Beghini A, Rahman Jamal, Hoe TS, Khoo A, Volpi L. An unusual intronic mutation in RECQ4 gene leading to Rothmund-Thomson syndrome. Mutation Research, 2002;508:99-103

Rahman Jamal, Taketani T, Taki T, Bessho F, Hongo T, Hamaguchi H, Horiike S, Taniwaki M, Hanada R, Nakamura H, Hayashi Y. Co-duplication of the MLL and FLT3 genes in patients with acute myeloid leukaemia. Genes, Chromosomes and Cancer, 2001;31:187-190.

Rahman Jamal, NSB Thomas, RE Gale, DC Linch. Variable expression of p16 protein in patients with acute myeloid leukaemia without gross rearrangements at the DNA level. Leukemia, 1996;10:629-636.

Research Grants (ongoing research grants) Project : Molecular studies on disease biomarkers and pathogenesis of cancer: Colorectal cancer as a model Source : National Biotecnology Directorate (06-05-01-003 BTK/ER 018) Amount : RM715,000 Project : Mechanisms that control glucocorticoid resistance in childhood acute lymphoblastic leukaemia Source : Fundamental Grant UKM (JJ-2004-01) Amount : RM200,000 Supervision of postgraduate students PhD 2 MSc 3 Professional activities (selected)

Interim Project Leader, Malaysian Cohort Study (MOSTI) Chairman, National Committee on Guidelines for Stem Cell Research Chairman, Expert Panel, National Thalassaemia Registry Member, Medical Qualifications Board, Malaysian Medical Council Adviser, Federation of Malaysian Thalassaemia Societies Treasurer, Malaysian Society of Paediatric Oncology



Professor Dr. Wan Zurinah Wan Ngah Qualifications BSc/Hons (UK), PhD (New Zealand)

PhD thesis in Biochemistry (awarded by Victoria University of Wellington, New Zealand, 1982)

Research interests

Anti-oxidants in ageing and Down syndrome as a model for accelerated ageing Palm oil vitamin E specifically gamma-tocotrienol in immune response, as a signalling molecule. Human, animal

and in vitro studies using gamma-tocotrienol are ongoing Studies into natural and synthetic compounds and their effect on cells in culture and in induced

hepatocarcinogenesis Selected publications

Then SM, Musalmah M, Sanfeliu C, Abdul Gapor MT and Wan Ngah WZ. Neuroprotective effect of -tocotrienol against hydrogen peroxide-induced oxidative stress in astrocytes culture. Mal J Biochem Mol Biol 2004; 9:22-27

Shamaan NA, Jarien Z, Khalid BAK, Top AGM and Wan Ngah WZ. Effect of Lingzhi and vitamin E supplementation on enzyme activities in the liver and plasma of normal and chemically induced hepatocarcinogenic rats. Intl J Medicinal Mushroom 2000; 2: 131-139

Shamaan NA, Wan Ngah WZ, Jarien Z, Abdullah Sani H, Jantan I, Khalid BAK. Danazol and clomiphene reduced the severity of hepatocarcinogenesis in the rat in vivo and cancer cells in vitro. Asia Pacific J Pharmacol 2000; 14: 67-72

Shamaan NA, Khalid BAK, Rahmat A and Wan Ngah WZ. Vitamin C and Aloe vera supplementation protects from induced chemical hepatocarcinogenesis in the rat. Nutrition 1998; 14: 846-852

Makpol S, Shamaan NA, Jarien Z, Md. Top AG, Khalid BAK and Wan Ngah WZ. Different starting times of vitamin E supplementation and tumour marker enzyme activities in the rat chemically induced with cancer. Gen. Pharmacol 1997; 28:589-592.

Research Grants (Selected ongoing research grants)

Project : Role of antioxidants in preventing degenerative damage in ageing and Down syndrome Source : Ministry of Science, Technology and Innovation (IRPA Top down PR Programme Leader) Amount: RM3,364,000 Project : HNF4 alpha gene polymorphisms in Type 2 diabetes mellitus (Project Leader) Source : Fundamental Grant UKM (JJ-2004-02) Amount : RM164,000 Project : The molecular mechanism of gamma-tocotrienol in hepatocarcinogenesis involving prenyl

pyrophosphatase and farnesol signal transduction pathway (Co-researcher) Source : IRPA 06-02-02-1035EAR Amount: RM102,000 Supervision of postgraduate students PhD 8 MSc 6 Professional activities (selected)

Editor Malaysian Journal of Biochemistry and Molecular Biology Member Technical Committee of National Biotechnology Directorate Member New York Academy of Science Member of Ministry of Education Nobel Laureate path committee



Professor Dr. Ramelah Mohamed Qualifications: B.Sc.; M.Sc. (ITB); Dip. Med. Micro. (Philippines); Ph.D (London)

PhD thesis in Medical Microbiology (awarded by University of London in 1984) Research interests

Infectious Diseases Our group has been interested in immunology and molecular biology of several infectious diseases.

Helicobacter pylori: Our group interest in H. pylori focuses on characterization of genes and their association with severe gastroduodenal diseases in different ethnic groups. It is interesting to note that genes associated with severe gastroduodenal diseases present in H. pylori strains varies with geographical regions and population. We have discovered three different variants of the cagA gene present among our Malaysian H. pylori strains, that is Type A (621-651 bp) AY769770, Type B (732-735 bp) AY769771 and Type C (525 bp) AY769772. In addition to genotyping, we are engaged in a study on the mechanisms of antibiotic resistance of H. pylori by looking at amplification or deletion of antibiotic resistance genes and expression of protein responsible for antibiotic resistance. Our group is also looking into the T-cell and antibody responses in patients infected with H. pylori.

Vibrio cholerae: During the last three years we collaborate with research groups from five different institutions (USM, UPM, UM, IMU & IMR) focusing on the development of rapid diagnostic tests for several diarrhoeal diseases particularly relying on PCR-based assays. Our group (UKM) has developed a Multiplex PCR for identification of V. cholerae in clinical specimens.

Hospital Acquired Infections

Our area of interest is looking into the mechanisms of carbapenam resistant in Pseudomonas aeruginosa isolated from various clinical specimens.

Selected publications (not more than 5)

Ramelah Mohamed, Aminuddin A, Alfizah H, Isa MR, Jasmi AY, Tan HJ, A.Rahman AJ, Rizal AM & Mazlam MZ. cagA gene variants in Malaysian Helicobacter pylori strains isolated from patients of different ethnic groups. Federation of European Microbiological Societies Immunology and Medical Microbiology 2005 (in press)

R Mohamed, A Hanafiah, A Ahmad, I Mohamed Rose, I Sagap, S Nadesan, AR A Jamal, S A Abdullah, M R Abdul Manaf, & J Ali Yaakob. Diversity of Helicobacter pylori cagA and vacA genes among strains isolated from multiracial patients in Malaysia. Helicobacter 2004;9 (5):512

H Alfizah, A Aminuddin, MR Isa, I Sagap, AY Jasmi, AM Rizal & M Ramelah. Clinical Relevance of the babA2-Positive Helicobacter pylori Isolates in Malaysia. Med. J. Malaysia 2004;59 (Supp C) :39.

Ramelah M, Alfizah H, Tan HJ, Jasmi AY, Isa MR & Aminuddin A. Genomics of Helicobacter pylori: An Indicator For Severity Of Gastroduodenal Diseases. 37thJoint Conferece of US-Japan on Cholera and Other Bacterial Enteric Infections Panel. Okinawa, Japan, 17-19 December 2002 : pp 138-144.

Ramelah M, M Azreen, MZ Noraziah & Rohani MY. 2002. Toxin Genes of Vibrio cholerae in Malaysia. 37thJoint Conference of US-Japan on Cholera and Other Bacterial Enteric Infections Panel. Okinawa, Japan; 17-19 December 2002 : pp 153-156.

Research Grants (ongoing research grants) Project : Helicobacter pylori Infection and Host Response: Genomics and Molecular Approach to Pathogenesis and Host Response Source : Ministry of Science, Technology and Innovation (IRPA Top down PR Programme Leader - 06-02-02-0000 PR0073/5 Amount : RM2,634,520 Project : Development of Diagnostic Tests for Vibrio cholerae : A Genomic Approach Source : Ministry of Science, Technology and Innovation (IRPA - 06-02-09-1001-PR00) Amount : RM 203,132 Project : Mechanism of Carbapenam Resistant Pseudomonas aeruginosa in Hospital Acquired Infection Source : Ministry of Science, Technology and Innovation (IRPA - 06-02-02-0047-EA232) Amount : RM 236,800 Supervision of postgraduate students Ph.D 2 M.Sc 4 Professional activities (selected)

Treasurer, MINDS UKM Chapter Life Member, Malaysian Society of Infectious Diseases and Chemotherapy Member, Academy of Medicine Malaysia. Member, Malaysian Graduate Society Member, Persatuan Perubatan Islam Malaysia



Associate Professor Dr. Roslan Harun Qualifications: MD (UKM), MRCP (UK), PhD (Leeds), FRCP (Glasgow), AM (Malaysia)

PhD thesis in Molecular Biology (awarded by the University of Leeds in 1998)

Research interests

Gene expression analysis in asthma Asthma is a chronic inflammatory disease of the airways with ongoing repair process that constitute airway wall remodeling. In this project, whole genome analysis using DNA microarrays is used to determine altered gene expression in various types of inflammatory and structural cells involved in the chronic airway inflammation and airway remodeling in asthma. These differentially expressed genes will be used to construct a specialized microarray, ‘Asthma chip’, which will be used to (a) identify gene expression changes underlying asthma; (b) generate molecular portraits for distinct types of asthma; and (c) correlate distinct gene expression profiling with various clinical manifestations of asthma.

Functional genomic of lung cancer Lung cancer is one of the leading causes of cancer death in worldwide and non-small cell lung cancer (NSCLC) accounts for nearly 80% of the disease. Understanding molecular changes in these major tumour types will allow identification of novel tumour markers or potential therapeutic targets. Using differential display and DNA microarray methods, we identified many differentially expressed genes in lung cancers isolated from bronchial biopsies. The expressions of some of these genes were subsequently confirmed by semi-quantitative or real-time RT-PCR. We are currently looking at the protein expression, determining the functions of these genes using RNA interference (RNAi) and studying the effects of these genes inhibition on the expression of the down-stream genes.

Selected publications Rahman Jamal, Baizura Jamaluddin, Idris Nor, Hamidah Alias, Jeffrey Abu Hassan, Roslan Harun. Abnormalities in

lung function among multiply transfused thalassaemia patients: Results from a thalassaemia daycare centre in Malaysia. South East Asian Journal of Tropical Medicine and Public Health 2005;36:1-5.

Fadilah SAW, Cheong SK, Roslan Harun, Rozie HM, Goh KY. GATA-1 and GATA-2 gene expression is related to the severity of dysplasia in myelodysplastic syndrome. Leukemia 2002; 16: 1563-1564.

Jeffrey AH, Saadiah S, Roslan Harun, Zainuddin MZ. Bronchodilator response to inhaled beta-2-agonist and anticholinergic drugs in patients with bronchiectasis. Respirology 1999; 4: 423-426.

Roslan Harun, Smith KK, Leek JP, Markham AF, Norris A, Morrison JFJ. Characterization of human SHC p66 cDNA and its processed pseudogene mapping to Xq12-q13.1. Genomics 1997;42: 349-352.

Roslan Harun, Markham AF, Morrison JFJ. Identification of differentially expressed genes in CD 19+ve B lymphocytes in allergic asthma. Advances in Experimental Medicine and Biology 1996; 409: 375-380.

Research Grants (ongoing)

Project: Identification and analysis of genes underlying allergic diseases using DNA microarrays Source: National Biotecnology Directorate (06-02-02-007 BTK/ER/32) Amount: RM 1,698,240.00 Project: Functional analysis of novel lung carcinoma genes using RNA interference and microarray analysis. Source: IRPA 06-02-02-0064-EA305 Amount: RM227,080

Supervision of postgraduate students

PhD 1 MSc 1 Professional activities (selected)

Member, Malaysian Thoracic Society (MTS) Member, Islamic Medical Association Malaysia (PPIM) Committee Member, Malaysia Drug Control Authority (DCA) Committee Member, Malaysian Society for the Advancement of Pharmacogenomics (MSAP) Life Member, Malaysia Medical Relief Society (MERCY Malaysia)



Associate Professor Dr. Ruszymah Hj. Idrus Qualifications MD (UKM), PhD (UKM)

PhD thesis in Physiology (awarded by Universiti Kebangsaan Malaysia in 1996)

Research interests Molecular genetics of deafness ● Tissue engineering of Cartilage, Bone and Skin Selected publications B H I Ruszymah. Autologous human fibrin as the biomaterial for tissue engineering. Medical Journal of Malaysia

2004; 59 : 30-31 (Suppl. B). K H Chua, B S Aminuddin, N H Fuzina , B H I Ruszymah. Interaction between Insulin-like growth factor-1 with other

growth factors in serum depleted culture medium for human cartilage engineering. Medical Journal of Malaysia 2004; 59 : 7-8 (Suppl. B)

M Y Phang, M H Angela Ng, K K Tan, B S Aminuddin, B H I Ruszymah, O Fauziah. Evaluation of Suitable Biodegradable Scaffolds for Engineered Bone Tissue. Medical Journal of Malaysia 2004; 59 : 198-199 (Suppl. B)

Aminuddin BS, Chua KH, Fuzina NH, Farah Wahida I, Mazlyzam AL, Norazlinda MD, Munirah S, Angela Ng MH, Lokman BS and Ruszymah BHI. Formation of Tissue Engineered Human Cartilage from Nasal Septum. KPJ Medical Journal 2003; 1(1): 3-7.

Ruszymah BHI, Chua KH, Mazlyzam AL, Fuzina NH and Aminuddin BS. Human serum verses animal serum in human cartilage tissue engineering. International Medical Journal 2003; 2(2)

Research Grants (ongoing) Project: Tissue Engineering of Human Tissues for Future Clinical Applications Source: 06-02-02-003/BTK/ER 022 Amount: RM2,200,000 Project: Formation of Human Cartilage via Tissue Engineering Method for Replacing Tissue Lost Source: 06-02-02-0037-EA189 Amount: RM223,384 Supervision of postgraduate students PhD 3 MSc 2 Professional activities 1. President - Tissue Engineering Society of Malaysia (TESMA) 2. Member - Tissue Engineering Society International (TESi) 3. Member - Electron Microscopy of Malaysia



Associate Professor Dr. Mohd Zaki Salleh Qualifications: BSc/Hons (UKM), MSc (London), PhD (USM) PhD thesis in Medical Microbiology (awarded by Universiti Sains Malaysia in 1994) Research interests

Molecular diagnosis of infectious diseases Specific proteins expressed by Shigella spp. and Campylobacter jejuni have been identified. Amino acid and nucleic acid sequences of the proteins were determined. The genes encoding the proteins were cloned to produce recombinant proteins. Protein-based and DNA-based diagnostic tests are being developed to detect both bacteria directly in clinical specimens. Similar approach is being used for nosocomial pathogens such as Staphylococcus spp. and Klebsiella spp.

Pharmacogenomics

Development of cheaper methods (PCR based) to determine genetic variants of P450 drug metabolizing enzymes (including CYP2C8, CYP2C9, CYP2C19, CYP3A4, CYP2D6) and some drug receptors (Beta2-adrenagic,dopamine-2 and mu-opiod) as well as drug transporter P-glycoprotein encoded by MDR1. We also focus on establishing genetic data bank for local population because of the large interethnic and intergeographical differences in genetic background which may implicate pharmacotherapeutic approach such as drug dosing and adverse effect.


Muthiah YD, Lee WL, Teh LK, Ong CE, Salleh MZ, Ismail R. A simple multiplex PCR method for the concurrent detection of three CYP2C8 variants. Clin Chim Acta 2004;349:191-198

Romaino SM, Teh LK, Zilfalil BA, Thong CP, Ismail AA, Amir J, Salleh MZ, Noor AP, Ismail R. A simple and rapid genotyping method for beta-2 receptor (β2AR) gene using allele specific multiplex PCR. J Clin Pharm Ther, 2004;29(1):47-52

MD Kamaliah, Y Azril Haris, S Mohd Zaki. Role of topical mupirocin as a prophylaxis in catheter related infections in haemodialysis patients. International Medical Journal, 2004; 11(1):41-46

Zainuddin Z, Teh LK, Suhaimi AW, Salleh MZ, Ismail R. A simple method for detection of CYP2C9 polymorphisms:nested allele specific-multiplex polymerase chain reaction. Clin Chim Acta 2003;336:97-102

N Jackson, M Zaki, AR Rahman, M Nazim, WN Win, S Osman. Fatal Campylobacter jejuni infection in a patient splectomised for thalassaemia. J Clin Pathol 1997; 50:436-437 Research Grants (ongoing research grants)

Project : Development of rapid diagnostic test(s) for Campylobacter jejuni Source : IRPA (06-02-05-1003/PR0016/06-01) Amount : RM262,000

Project : Development of rapid diagnostic test(s) for Shigella spp. Source : IRPA (06-02-05-1003/PR0016/06-02)

Amount : RM238,000 Project : Development of diagnostic tests for enteric pathogens : a genomic approach Source : National Biotechnology Directorate (304-UINOMED.6150046)

Amount : RM 500,500 Supervision of postgraduate students PhD 1 MSc 4 Professional activities

1. Vice-President, Malaysian Society for the Advancement of Pharmacogenomics 2. Member, Malaysian Society of Infectious Diseases and Chemotherapy 3. Member, Malaysian Society of Microbiology



Associate Professor Dr. Syed Zulkifli Syed Zakaria Qualifications: MD (UKM), M.Med. Paeds. (UKM), PhD

PhD thesis in Clinical & Molecular Epidemiology (awarded by University of Newcastle, Australia in 2003)

Research Interest a. Molecular Epidemiology:

Epidemiology of biomarkers including drug metabolizing enzymes, MTHFR genes and DNA repair genes. Mutation of the RET gene in Hirchprung’s disease

b. Oncology Genetic markers in cancer including bladder, lung and leukemias.

c. Clinical Focusing on quality of life of thalassemics and leukemia survivors.

Current research activities

1. Evaluation of the knowledge, attitude and practice of Malaysian paediatricians in withdrawing or limitation of treatment of children.

2. Comparison study of the health related quality of life(HRQOL) between paediatirc leukemia population on maintenance therapy and those post chemotherapy: a cross sectional study.

3. Parental stress index and CISS score in parents of patients with thalassemia. 4. Characterisation of RET gene mutations in Malaysian children with Hirschsprung’s disease.


Rahman Jamal, Fazlina N, Maha A., Zarina A.L., Hamidah A, Zulkifli SZ, Cheong S.K. Ainoon O., Hamidah

N.H., Multidrug resistance proteins (MDR) in acute leukemia cases in a typical hematology-oncology unit in Malaysia. Pediatric Blood and Cancer 2004;43:S1-394

Zulkifli SZ, Ong LC, Boo NY, Lu AIC, Zairul Azwan MA, Suryani MZ, Roshidah R, Krishnalatha B, Afizah S, Siti Aishah AM, Azlina AR, Nurul Hidayati Z, Nanitha K, Arizal TA. An audit of hand washing practices among health-care providers in a neonatal intensive care. Asian Oceania Journal of Pediatrics and Child Health 2003;2(1)

Zarina Latiff, Zulkifli SZ, Rahman Jamal. Risk assessment and microbiological profile of infections in paediatric cancer patients with febrile neutropenia. Malaysian Journal of Paediatrics and Child Health 2000

N.Y.Boo, N.C. Wong, Zulkifli SS, M.S.Lye. 1999. Risk factors associated with umbilical vascular catheter-associated thrombosis in newborn infants. J Paediatric Child Health 1999;35:460-465.

Rahman Jamal, Fadzillah G, Zulkifli SZ, Yasmin M. Seroprevalence of hepatitis B, hepatitis C, CMV and HIV in multiply transfused thalassaemia patients : Results from a thalassaemia day care centre in Malaysia. South East Asean Journal of Tropical Medicine and Public Health 1998;29:64-69

Supervision of postgraduate students

PhD 1 M.Med (Paeds) 5

Professional activities

Member, Medical Faculty Research and Ethics Committee Member, Malaysian Association for the Protection of Children



CURRENT INFRASTRUCTURE AND KEY SYSTEMS UMBI management office and laboratory are located at 7th floor, Clinical Block, Hospital Universiti Kebangsaan Malaysia (HUKM), Cheras, Kuala Lumpur. A well equipped genomic laboratory, tissue engineering laboratory, a cancer genetic laboratory and Biobank HUKM-UMBI has been set up for researchers in UMBI and Faculty of Medicine to conduct research in molecular medicine. Current infrastructure at UMBI includes:

Varian System (Denaturing High Performance Liquid Chromatography for mutational analysis)

The DHPLC machine is an automated and high throughput system which can analyse 96 samples in 6.5 hours time. The technique is easy to perform, not labour intensive and time consuming.

ABI 3100 Genetic Analyzer (DNA sequencing)

The ABI 3100 Genetic Analyzer with 16 capillary electrophoresis system can separate, detect and analysed 16 samples in 2.5 hours with a simple and easier method. Besides DNA sequencing, resequencing technique and linkage analysis can also be performed by this machine.

iCycler Real-Time Polymerase Chain Reaction Machine

iCycler Real Time – PCR machine is a real time PCR thermocycler with optical module and provide a real time measurement of the PCR proses in 2 – 2.5 hours time. Real time and on line PCR monitoring also reduces opportunities and speeds time-to results because traditional post PCR steps are no more necessary.

MVE Cryosystem (BioBank)

The MVE cryosystem consists of a liquid nitrogen freezer with storage capacities of 2000 vials and a liquid nitrogen tank.

Microarray System (Microarray)

The affymetrix microarray system provide RNA expression profiling using GeneChip Human Genome arrays. The system includes a hydridization system, scanner and operating system with GCOS software for data analysis and mining. GeneChip Human Genome Arrays provide gene expression data for a multitude of applications, eg: uncovering new regulatory pathways and validating drug targets

All researchers within UKM are allowed to use the facilities at UMBI with prior approval from UMBI.



EVENTS IN 2004 Molecular Research Update Date Presenter Title 19 January Zulhabri Osman Telomerase activity In human colorectal cancer.

Rosmadi Mohd Yusoff Identification of differentially expressed genes in non-small cell lung cancer by mRNA differential display and microarray analysis.

16 February Farah Wahida Ibrahim Genetic linkage analysis in families with non-syndromic hereditary hearing

impairment. Azian Abd. Murad Low density lipoprotein (Ldl) receptor gene mutation in patients with familial

hypercholesterolaemia (FH): Molecular and clinical characterization. Farah Wahida Ibrahim Technique of the month – Genetic linkage analysis. 15 March Fazlina Nordin Multidrug resistance (MDR) proteins in acute leukaemia cases in Hospital

UKM. Chua Kien Hui Developing human chondrocyte growth medium for cartilage tissue

engineering. 19 April Mazlyzam Abdul Latif Evaluation analysis of tissue engineering human skin. Suhana Mamat Genotyping of detoxification enzyme polymorphism as an indicator to cancer

risk. 17 May Farizan Arif, UiTM Upregulated genes in acute myeloid leukemia. Rosmadi Yusof Technology review - RNA interference. 14 June Munirah Saa'ban Tissue Engineering & Human Genetics Laboratory, HUKM Chua Kien Hui Tissue Engineering & Human Genetics Laboratory, HUKM 16 August Farzin Farzaneh Gene therapy and immunotherapy for cancer : hype or hope? Functional analysis of the genome - the post genomic challenge. 20 September Khairul Bariah Ahmad Transfection of p53 wild type gene into various HCC cell Amin Noordin (IMR) lines. Alfizah Hanafiah Helicobacter pylori cagA and vacA genes variants among strains isolated

from multiracial patients in Malaysia 25 October Angela Ng Min Hwei Comparison of the different cell sources for bone tissue engineering. Melati Khalid Mutation detection using dHPLC. 6 December Norazril Shamsul Evaluation of chitosan-based scaffold as a biomaterial for tissue

engineering. Erica Choong Pei Feng In vitro differentiation of human bone marrow mesenchymal stem cells into

neuron-like cells.

Dinner in honour of Prof. Farzin Farzaneh Molecular update held monthly



Lecture Series Date Presenter Title 13 March Assoc. Prof Dr. Roslan Harun Basics of Molecular Biology Assoc. Prof Dr Ruszymah Hj Idrus PCR, Sequencing, DNA Extraction

Update on new technologies organized by UMBI Date Programme 30 March Workshop on Laser Microdissection (BioSynTech Sdn. Bhd) 27 April Workshop on Automated Processing and Analysis of Microarrays using the TECAN LC

Scanner (Siber Hegner (M) Sdn. Bhd) 7 May Microarray Technology (Applied Biosystem) 19 May Workshop on RNA Interference (BD Biosciences Clontech) 27 May Novel Microarray Technology : Production scale genotyping and gene expression analysis

using the Illumina BeadStation® System 9 December Beckman Coulter CEQ8000 DNA Analysis System 14 December Biosafety for Level II & Level III (Interscience (M) Sdn Bhd) 30 December Microarray System (Perkin Elmer Sdn Bhd)

Workshop on Microarray Technology

SPSS (Statistical package and training) Workshop Date Programme 27-28 March Basic & Advanced Session 9-10 October Basic & Advanced Session



Conferences, Workshops, Exhibitions, Collaborations Etc. Date Programme 16-18 June Seminar and workshop on ‘Update on Helicobacter pylori’ 19 August Visit by Innobiologics Sdn. Bhd. 16-17 August Visit by Prof. Dr. Farzin Farzaneh, Scientific Advisor of UMBI 15-18 August UKM Exposition on Research and Innovation 2004 9 December Hari Raya Aidilfitri celebration, Kompleks Perubatan UKM 2004 12-13 December Seminar Pendedahan Kursus Kesihatan Kebangsaan 14-16 December Pameran Sempena Konvensyen Perdana - Organised by Biro Tatanegara, Jabatan Perdana

Menteri

UMBI’s participation at the UKM Research & Innovation Expo, 15-18 July 2004, Dewan Canselor Tun Abdul Razak, UKM



OTHER ACTIVITIES OF RESEARCHERS Activity The Malaysian-Swedish Dialogue in Medical Research organized by the Academy of Science Malaysia Date 5-6th February Venue: Legend Hotel, Kuala Lumpur Attended by: Prof. Dr. A Rahman A Jamal, Prof. Dr. Wan Zurinah Wan Ngah, Prof. Dr. Ramelah Mohamad,

Assoc. Prof. Dr. Roslan Harun

Activity Consolidation of teamwork and creativity for leaders Date 13-15th February Venua Guoman Resort and Hotel, Port Dickson, Negeri Sembilan Attended by: Professor Dr. A Rahman A Jamal Activity Retreat on ‘UKM as a Research University’ Date 27-29th February Venue Regency Hotel, Port Dickson, Negeri Sembilan Attended by: Professor Dr. A Rahman A Jamal Paper Generation of research funds Activity Seminar on ethics, medicine and the law: theories and contemporary issues Date 13th Mac 2004 Venue Renaissance Hotel, Kuala Lumpur Attended by Professor Dr. A Rahman A Jamal Activity 1st National Workshop and Colloquium on Pharmacogenetics and visit to INFORMM USM Date 1-2nd April Venue School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian, Kelantan Attended by Professor Dr. A Rahman A Jamal and Assoc. Prof. Dr. Syed Zulkifli Syed Zakaria Activity Retreat for members of the Faculty of Medicine, UKM Date 13-15th April 2004 Venue Guoman Resort and Hotel, Port Dickson, Negeri Sembilan Attended by Professor Dr. A Rahman A Jamal Paper ‘Towards excellence in research in the faculty’ Activity Workshop on post-graduate supervision Date 11-12th May 2004 Venue Equatorial Hotel, Bangi Attended by Professor Dr. A Rahman A Jamal Paper ‘Optimising supervision of post-graduates’ Activity International Public Lectures: USM-Cuba International Scientific Colloquium in Conjuction with Cuban

Week Date 31st May 2004 Venue USM Kubang Kerian Kelantan Attended by Associate Professor Dr Mohd Zaki Salleh Activity Visit by delegation from the Ministry of Research and Technology, Indonesia to UKM Date 21st Jun 2004 Venue UKM, Bangi Attended by Professor Dr. A Rahman A Jamal Discussion Focus on Bioethics Activity Workshop on curricullum review Date 4-7th July 2004 Venue Corus Paradise Hotel, Port Dickson Attended by Professor Dr. A Rahman A Jamal and Professor Dr. Wan Zurinah Wan Ngah Activity Convention for deputy vice-chancellors, deans and directors of institutions of higher learning Date 21-22nd July 2004 Venue Pan Pacific Hotel, KLIA Attended by Professor Dr. A Rahman A Jamal



Activity Workshop on documentation for ISO 9001:2000 Date 27-28th July 2004 Venue Marriot Hotel, Putrajaya Attended by Professor Dr. A Rahman A Jamal Activity Workshop on scientific writing for young lecturers in the Faculty of Medicine, UKM Date 24th August 2004 Venue Crown Princess Hotel, Kuala Lumpur Attended by Professor Dr. A Rahman A Jamal Paper How to transform research into papers Activity Seminar on ‘Molecular Genetics’ Date 24-26th September 2004 Venue School of Medical Sciences, Universiti Sains Malaysia, Kubang, Kerian, Kelantan Attended by Professor Dr. A Rahman A Jamal Paper Cancer genetics Activity ESGH XVII International Workshop Gastrointestinal Pathology and Helicobacter Date 22-24th September 2004 Venue Vienna, Austria Attended by Professor Dr. Ramelah Mohamed Activity Research dialogue between researchers from the faculties of Engineering and Medicine and UMBI Date 25th September 2004 Venue Faculty of Medicine UKM Attended by Assoc. Professor Dr. Roslan Harun Activity National Diagnostic Conference and Workshop Date 4-7th October 2004 Venue INFORMM, Universiti Sains Malaysia, Kubang, Kerian, Kelantan Attended by Assoc. Professor Dr. Mohd Zaki Salleh Activity Visit by delegation from Ministry of Science, Technology and Innovation, Innobio and Burrill and Co. Date 19th October 2004 Venue UMBI Attended by Director & Fellows Activity Presentation of the final draft of the National Policy on Biotechnology Date 6th Disember 2004 Venue Marriot Hotel, Kuala Lumpur Attended by Professor Dr. A Rahman A Jamal Activity INFORMM Postgraduate Workshop Series (3): Beyond R & D Date 12th December 2004 Venue USM Kubang Kerian, Kelantan Attended by Associate Professor Dr. Mohd Zaki Salleh Discussion Career opportunities in biomedicine and medical biotechnology Activity Visit to the Fred Hutchison Cancer Research Centre in Seattle, Washington, USA Date 19-25th December 2004 Venue Fred Hutchison Cancer Research Centre in Seattle, Washington, USA Attended by Professor Dr. A Rahman A Jamal



POST GRADUATE RESEARCH STUDENTS IN 2004 Doctor of Philosopy

Name Project Title Supervisors from UMBI

Year of registration

Aini Suzana Signal transduction mechanisms of the antiproliferative effect of �-tocotrienol of liver cancer cell lines


1998

Khairul Bariyah Ahmad Amin Nordin

Detection of p53 mutations hepatocellular cancer patients using SSCP


1999

Norwahidah Abdul Karim Apoptosis and effects of �-tocotrienol and �-tocopherol on liver cancer cells (HepG2)

Prof. Dr. Wan Zurinah Wan Ngah 2000

Zulhabri Othman Detection of p53 and K-Ras2 gene mutations and measurement of aflatoxin B1-albumin adduct level and telomerase activity in human hepatocellular carcinoma and colorectal cancer

Prof. Dr. Wan Zurinah Wan Ngah Prof. Dr. A Rahman A Jamal

2000

Rosmadi Yusof Cloning and characterization of novel lung cancer genes by combining the techniques of mRNA differential display, oligonucleotide microarray and RNA interference

Assoc. Prof Dr. Roslan Harun Assoc. Prof Dr. Ruszymah Hj. Idrus

2001

Mazlyzam Abdul Latiff Formation of tissue engineered human skin for clinical application and future commercialization

Assoc. Prof Dr. Ruszymah Hj. Idrus

2002

Alfizah Hanafiah Genomic characterization and functional analysis of the tyrosine phosphorylation motif in Cag A protein in Helicobacter pylori strain

Prof. Dr Ramelah Mohamed Prof. Dr. A Rahman A Jamal

2002

Wan Noraini Wan Yusuf Carbapenam resistant Psedumonas aeruginosa in hospital acquired infections

Prof. Dr. Ramelah Mohamed 2002

Kirnpal Kaur Banga Singh

Development of rapid diagnostic tests for Shigella flexneri : proteomic and genomic approach

Assoc. Prof Mohd Zaki Salleh,

2002

Angela Ng Min Hwei Formation of tissue engineered human bone in the presence of various growth factors and the cloning of the telomerase gene


2003

Haliansyah The influence of cytokines, liver tumour promoter and expression of selenoproteins


Mazatulikhma Mat Zain Neuroprotective effect of synthetic pyrollidone derivsatives and their mechanism of action


D. Maryama Ag Daud Effect of exercise duration and intensity on the oxidative status and antioxidant enzyme activities among sedentary adults


Riyadh Saif Ali Al Hammadi

HNF4 alpha gene polypmorphisms in Type 2 diabetes mellitus


Goon Jo Ann Effect of exercise(tai chi) on oxidative status in the ageing population


Mohd Syahril Mohd Zin

Mechanism that controls the glucocorticoid resistance in childhood acute lymphoblastic leukemia

Prof. Dr. A Rahman A Jamal 2004



Masters of Science

Name Project Title Supervisors from

UMBI Year of

registration Nor Azian Abdul Murad Molecular, biochemical and clinical characteristics of

the low density lipoprotein (LDL) receptor gene in patients with familial hypercholesterolaemia (FH) in the Malaysian population.

Prof. Dr. A Rahman A Jamal 2001 (Graduated in

2004)

Jeenath Banu Jamaluddin

Identification of differentially expressed gene in cord blood CD4+ T-helper lymphocyte in asthma

Assoc. Prof Dr. Roslan Harun 2002

Norazlinda Md Daud Identification of deafness gene locus in a family via genetic linkage analysis


2002

Munirah Sha’ban Effect of growth factors on the proliferation of animal and human articular chondrocyte and reconstruction of articular tissue via tissue engineering method for clinical application


2002

Fazlina Nordin Multidrug Resistance (MDR) proteins and in-vitro cytotoxicity of anti-leukemic drugs.

Prof. Dr. A Rahman A Jamal

2003

Melati Khalid APC gene mutation and methylation status in colorectal cancer

Prof. Dr. A Rahman A Jamal

2003

Maridah Mohd Amin Effect of ageing on antioxidant enzymes, oxidative stress, protein degradation and sister chromatid exchange


2003

Mohd Shahrul Azreen Mustaffa

Development of a multiple PCR for rapid diagnosis of Vibrio cholerae infections


Azni Amir Musa Cloning and expression of the 24.1 kDA protein for Campylobacter jejuni

Assoc. Prof. Dr. Mohd Zaki Salleh

2003

Suhaniza Sulaiman The effect of Chlorella vulgaris on hepatocarcinogenesis in the rat


2003

Suhana Md Saad The anticarcinogenic characteristics of chlorella vulgaris algae and its nutrient content


2003

Suhana Mamat Genetic polymorphisms of p450 and the effect of vitamin E on lipid peroxidation in cells expressing CYP2E1 enzyme


2003

Mohammad Johari Ibrahim

Effect of vitamin E supplementation on the oxidative status and immune response in ageing in the Malaysian population


2003

Lim Lay Cheng To study the oxidative stress products and antioxidant status in the ageing process


2004

Saadiah Abd. Malek PCR-based method for rapid diagnosis of systemic candidiasis

Prof. Dr Ramelah Mohamed Prof. Dr. A Rahman A Jamal

2004

Norhayati Kamaruddin Association of Helicobacter pylori with p53 protein expression and pepsinogen levels in gastric precancerous and cancerous lesions.


Wan Rasinah Zakariah Pencirian gen kerintangan antibiotik pada Helicobacter pylori yang dipencilkan daripada pesakit di Malaysia.


Norzie Azlin Pudzi Development of DNA-based diagnostic test for the detection C. jejuni and C. coli


2004

Nik Noorul Shakira Mohamed Shakrin

Development of DNA-based diagnostic test for the detection of Shigella spp.


2004

Mohd Khairi Johari Navigating the CYP2C8 allelic variations using denaturing high performance liquid chromatography (DHPLC)


2004



PUBLICATIONS Journal Publications

1. Rahman Jamal. Thalassaemia in Malaysia: Confronting the issues and mapping the strategies. Asian-Oceanian Journal of Pediatrics and Child Health 2004; 3(1): 7-15

2. Rahman AJ. Molecular medicine: the way forward. Med J Malaysia 2004; 59 (4): 441-442 3. Then SM, Musalmah M, Sanfeliu C, Abdul Gapor MT and Wan Ngah WZ. Neuroprotective effect of -tocotrienol

against hydrogen peroxide-induced oxidative stress in astrocytes culture. Mal J Biochem Mol Biol 2004; 9: 22-27

4. R Mohamed, A Hanafiah, A Ahmad, I Mohamed Rose, I Sagap, S Nadesan, AR A Jamal, S A Abdullah, M R

Abdul Manaf, & J Ali Yaakob. Diversity of Helicobacter pylori cagA and vacA genes among strains isolated from multiracial patients in Malaysia. Helicobacter 2004; 9 (5): 512

5. Muthiah YD, Lee WL, Teh LK, Ong CE, Salleh MZ, Ismail R. A simple multiplex PCR method for the concurrent

detection of three CYP2C8 variants. Clin Chim Acta 2004; 349:191-198 6. Romaino SM, Teh LK, Zilfalil BA, Thong CP, Ismail AA, Amir J, Salleh MZ, Noor AP, Ismail R. A simple and

rapid genotyping method for beta-2 receptor (β2AR) gene using allele specific multiplex PCR. J Clin Pharm Ther 2004; 29(1): 47-52

7. MD Kamaliah, Y Azril Haris, S Mohd Zaki. Role of topical mupirocin as a prophylaxis in catheter related

infections in haemodialysis patients. International Medical Journal 2004;11(1): 41-46 8. Noor Aini Abdul Hamid, Goon Jo Ann, Illyana Ismail, Wan Zurinah Wan Ngah, Abdul Gapor Md Top and

Musalmah Mazlan. Changes in oxidative status in rat’s heart with age as compared to the blood and effect of palm vitee supplementation. Malaysian Journal of Clinical Biochemistry 2004; 4:27-34

9. Noor Aini Abdul Hamid, Goon Jo Ann, Aminuddin Abdul Hamid Karim, Azian Abdul Latif, Nabishah Mohamad,

Zaiton Zakaria, Jamilah Baharom, Roshidah Hasan, Musalmah Mazlan and Wan Zurinah Wan Ngah. Effect of exercise (Tai Chi) on oxidative status among population in retirement age: A preliminary report Mal J Biochem Mol Biol 2004; 9: 31-34 10. Then Sue Mian, Musalmah Mazlan, Coral Sanfeliu, Abdul gapor Md Top and Wan Zurinah Wan Ngah.

Neuroprotective effect of -tocotrienol against hydrogen peroxide-induced oxidative stress in astrocytes culture. Mal J Biochem Mol Biol 2004; 9: 22-27

11. Ramelah M, Aminuddin A, Alfizah H, Isa MR, Jasmi AY, Tan HJ, Jamal R, Rizal AM, Mazlam MZ. cagA gene

variants in Malaysian Helicobacter pylori Strains Isolated from patients of different ethnic groups. FEMS Medical Microbiology and Immunology Journal (in press).

NOVEL FINDINGS Novel Gene/Variants Sequences Deposited in GenBank NCBI, USA

1. Ramelah M, Alfizah H, Aminuddin A (2004). HP 167 type A cag A, Accession no: AY769770

2. Ramelah M & Alfizah H, Aminuddin A (2004). HP 594 type B cag A, Accession no: AY769771 3. Ramelah M & Alfizah H, Aminuddin A (2004). HP 280 type C cag A, Accession no: AY769772

4. Nik Noorul Shakira, MS, Kirnpal-Kaur, BS, Ravichandran M, Asma I, Mohd Zaki, S (2004). Shigella boydii outer

membrane protein A precursor (ompA) gene, Accession no: AY590298



Abstracts in Journals

1. Rahman Jamal ,Fazlina N, Maha A, Zarina AL, Hamidah A, Zulkifli SZ, Cheong SK, Ainoon O, Hamidah NH..

Multidrug resistance proteins in acute leukaemia cases in a typical hematology-oncology unit in Malaysia. Pediatric Blood and Cancer 2004;43: S1-394.

2. H Alfizah, A Aminuddin, MR Isa, I Sagap, AY Jasmi, AM Rizal & M Ramelah. 2004. Clinical relevance of the

babA2- positive Helicobacter pylori isolates in Malaysia. Med. J. Malaysia 2004; 59 (Supp C): 39. 3. Y A Mohd Yusof, A H Harliansyah, M Noor Azian and W Z Wan Ngah. Effects of Zinziber officinale on

superoxide dismutase, glutathione peroxidase, catalase and glutathione content in HepG2 cell line. Free Radical Biology and Medicine 2004; 36 (Suppl 1): S133

4. S Makpol, Z A Bakkar, M Mazlan and W Z Wan Ngah. Effect of hydrogen peroxide and �tocotrienol on human lymphocyte proliferation with aging. Free Radical Biology and Medicine 2004; 36 (Suppl 1): S125 5. S M Then, M Musalmah M T Gapor and W Z Wan Ngah. Neuroprotective effects of � tocotrienol and �-

tocopherol against H2O2 induced oxidative stress in astrocytes culture. Free Radical Biology and Medicine 2004; 36 (Suppl 1): S89

6. I Nor Isnida, M Musalmah and W Z Wan Ngah. Effect of gamma-tocotrienol on DNA damage by Comet assay.

Free Radical Biology and Medicine 2004; 36 (Suppl )1: S83 7. A H Noor Aini, J A Goon, A H K Aminuddin, A L Azian, M Nabishah, Z Zaiton, B Jamilah, H Rosyida, M

Musalmah and Wan Ngah W Z. Effect of exercise (Tai Chi) on lipid peroxidation and antioxidant enzymes activity among population in retirement age group. Free Radical Biology and Medicine 2004; 36 (Suppl 1): S57

8. Zulhabri O, Wan Ngah WZ, Sagap I, Sukumar M, Melati K, Isa MR, Jamal AR. Telomerase activity in human

colorectal cancer. Med J Malaysia ; 59 (Suppl C): 81. 9. Philip R, Dinsuhaimi S, Rosdan S, Samsudin AR, Shamsuria O, Mohd Zaki S, Jamalulail SM. In vitro evaluation

of the growth enhancing or cytotoxic effect of Sticophus species (Gamat) on established human fibroblast cell lines and antimicrobial activity. Med J Malaysia 2004; 59 (Supp B): 95-96

10. B H I Ruszymah. Autologous human fibrin as the biomaterial for tissue engineering. Med J Malaysia. 2004;59

(Suppl. B): 30-31 11. A H M Y Badrul, B S Aminuddin, I Sharaf, O C Samsudin, S Munirah, B H I Ruszymah. The effects of

autologous human serum on the growth of tissue engineered human articular cartilage. Med J of Malaysia 2004; 59 (Suppl. B): 11-12

12. B Azmi, B S Aminuddin, I Sharaf, O C Samsudin, S Munirah, K H Chua, B H I Ruszymah. The significance of

using pooled human serum in human articular cartilage tissue engineering. Med J Malaysia 2004; 59 (Suppl. B) : 13-14

13. K H Chua, B S Aminuddin, N H Fuzina , B H I Ruszymah. Interaction between insulin-like growth factor-1 with

other growth factors in serum depleted culture medium for human cartilage engineering. Med J Malaysia 2004; 59 (Suppl. B) : 7-8

14. M H Angela Ng, B S Aminuddin, K K Tan, G H Tan, B H I Ruszymah. The use of bone marrow stem cells for

bone tissue engineering. Med J Malaysia 2004; 59 (Suppl. B) : 41-42 15. S Munirah, B S Aminuddin, K H Chua, N H Fuzina, M R Isa, B H I Ruszymah. Tissue engineered human

articular neocartilage using serial expanded chondrocytes. Med J Malaysia 2004;59 (Suppl. B) : 9-10 16. A L Mazlyzam, B S Aminuddin, B S Lokman, M R Isa, H Fuzina, O Fauziah, B H I Ruszymah. Quality

evaluation analysis of bioengineered human skin. Med J Malaysia 2004; 59 (Suppl. B): 39-40. 17. OC Samsudin, B S Aminuddin, S Munirah, K H Chua, N H Fuzina, M R Isa, B Azmi, A H M Y Badrul, B H I

Ruszymah. In vitro development of autologous tissue engineered human articular neocartilage for orthopaedic surgery. Med J Malaysia 2004;59 (Suppl. B) : 15-16



18. A N Nur Adelina, B S Aminuddin, S Munirah, K H Chua, N H Fuzina, L Saim, B H I Ruszymah. Phenotypic

expression of collagen type II and collagen type I gene in monolayer culture of human auricular chondrocytes. Med J Malaysia. 2004;59 (Suppl. B): 188-189

19. B H Muhd Fakhruddin, B S Aminuddin, A L Mazlyzam, B H I Ruszymah. The effects of age on monolayer

culture of human keratinocytes for future use in skin engineering. Med J Malaysia 2004; 59 (Suppl. B): 182-183 20. K K Tan , B S Aminuddin, G H Tan, M Sabarul Afian, M H Angela Ng, O Fauziah, B H I Ruszymah. Strategy for

generating tissue-engineered human bone construct. Med J Malaysia 2004;59 (Suppl. B) : 43-44 21. K H Chua, B S Aminuddin, N H Fuzina, B H I Ruszymah. Human serum provided additional values in growth

factors supplemented medium for human chondrocytes monolayer expansion and engineered cartilage construction. Med J Malaysia 2004; 59 (Suppl. B): 194-195

22. M M Norhayati, A L Mazlyzam, R Asmah , H Fuzina, B S Aminuddin, B H I Ruszymah, O Fauziah. Collagen

fibers an important entity in skin tissues remodeling. Med J Malaysia 2004; 59 (Suppl. B): 184-185 23. B S Shamsul, B S Aminuddin, M H Angela Ng, B H I Ruszymah. Age and gender effect on the growth of bone

marrow stromal cells in vitro. Med J Malaysia 2004; 59 (Suppl. B): 196-197 24. I Farah Wahida, B S Aminuddin, S Munirah, K H Chua, N H Fuzina, M R Isa, L Saim, B H I Ruszymah. Gene

expression characteristic in human auricular cartilage tissue engineering. Med J Malaysia 2004; 59 (Suppl. B) : 190-191

25. L Saim, B S Aminuddin, S Munirah, K H Chua, A Izuddin Fahmy , N H Fuzina, M R Isa, B H I Ruszymah.

Formation of tissue-engineered human auricular cartilage via tissue engineering technique for future use in ear surgery. Med J Malaysia 2004; 59 (Suppl. B) : 192-193

26. S A H Norazril, B S Aminuddin, M M Norhayati, A L Mazlyzam, O Fauziah, B H I Ruszymah. Comparison of

chitosan scaffold and chitosan-collagen scaffold: A preliminary study. Med J Malaysia 2004;59 (Suppl. B): 186-187

27. M Y Phang, M H Angela Ng, K K Tan, B S Aminuddin, B H I Ruszymah, O Fauziah. Evaluation of suitable

biodegradable scaffolds for engineered bone tissue. Med J Malaysia 2004; 59 (Suppl. B): 198-199

Proceedings

1. Wan Zurinah Wan Ngah. Peranan antioksidan dalam menghalang kerosakan degeneratif terhadap Sindrom Down dan penuaan. Prosiding Seminar IRPA RMK-8 2004;1:177-185

2. Wan Zurinah Wan Ngah. Kajian kemointervensi menggunakan antioksidan vitamin E untuk mengurangkan

stres oksidatif pada proses penuaan. Prosiding Seminar IRPA RMK-8 2004; 1: 86-193

3. Lim L. C., Azian A. L., Nor Aini A.H., Musalmah M., Yasmin A., Zaiton Z., Nabishah M., Aminudin A.K., Normiza R., Yusfairuz M. P., Noor Laili M.T., Rosyida H., Jamilah B., Norhana Y., Fatimah M. & Wan Ngah W.Z. Kesan umur ke atas aktiviti enzim katalase di kalangan penduduk Malaysia. Prosiding Seminar IRPA RMK-8 2004; 2:46-51

4. Ibahim M.J., Shahnaz M., Azian A.L., Noor Aini A.H., Noor Laili T., Zaiton Z., Aminudin A.K., Nabisha M., Yasmin

A., Musalmah M., Chin S.F., Jamilah B., Rosyida H. & Wan Ngah W.Z. Perbandingan tahap respon imun dengan perubahan peringkat umur : Laporan Prelim. Prosiding Seminar IRPA RMK-8 2004; 2: 52-55

5. Normiza R., Azian A.L., Noor Laili M.T., Noor Aini A.H., Norhana Y., Fatimah M., Yasmin A., Musalmah M.,

Zaiton Z., Aminudin A.K., Nabishah M., Jamilah B., Rosyida H., Yusfairuz M.P., Ibahim M.J., Lim L.C., Chin S.F. & Wan Ngah W.Z. Penentuan ujian fungsi hepar, profil lipid dan renal serta kiraan sel darah dalam kajian proses penuaan penduduk di Malaysia. Prosiding Seminar IRPA RMK-8 2004; 2:56-60

6. Yusfairuz M. P., Normiza R., Azian A.L., Noor Laili M.T., Noor Aini A.H., Norhana Y., Fatimah M., Yasmin A.,

Musalmah M., Zaiton Z., Aminudin A.K., Nabishah M., Jamilah B., Rosyida H., Ibahim M..J., Lim L.C., Chin S.F. & Wan Ngah W.Z. Perkaitan antara profil lipid dengan indeks jisim tubuh dan umur dalam kajian proses penuaan di kalangan penduduk Malaysia. Prosiding Seminar IRPA RMK-8 2004; 2:61-66



7. Chin S.F., Azian A.L., Noor Aini A.H., Noor Laili M.T., Zaiton Z., Aminudin A.K., Nabishah M., Yasmin A.,

Musalmah M., Ibahim M.J., Shahnaz M.J., Jamilah B., Rosyida H. & Wan Ngah W.Z. Kesan umur dan kerosakan DNA di kalangan masyarakat di sekitar Lembah Klang. Prosiding Seminar IRPA RMK-8 2004; 2:67-70

8. Noor Aini A.H., Goon J.A., Aminudin A.H.K., Azian A.L., Nabishah M., Zaiton Z., Musalmah M. & Wan Zurinah

W.Z. Kesan senaman (Tai Chi) ke atas status oksidatif di kalangan populasi dalam umur persaraan. Prosiding Seminar IRPA RMK-8 2004; 2:71-75

9. Noor Aini A.H., Nafeesa Najwa M.K., Aminudin A.H.K., Azian A.L., Nabishah M., Zaiton Z., Musalmah M. & Wan

Zurinah W.N. Mengkaji kesan senaman Tai Chi ke atas respon imun dan melihat korelasinya dengan stres oksidatif di kalangan populasi dalam umur persaraan. Prosiding Seminar IRPA RMK-8 2004; 2:76-79

10. Aishah Adam, Laila Mehat, Maziana Mahamood, Mizaton H. Hasan, Noor Aini A. Hamid & Wan Zurinah Wan

Ngah. Kerosakan DNA pada Trisomi 2. Prosiding Seminar IRPA RMK-8 2004; 2:80-83

11. Aishah Adam, Laila Mehat, Maziana Mahamood, Mizaton H. Hasan, Caroline Sunggip, Mohd Alimukhti Mansor, Alini Marzuki, Noor Aini A. Hamid & Wan Zurinah Wan Ngah. Status stres oksidatif di kalangan Trisomi 21. Prosiding Seminar IRPA RMK-8 2004; 2: 84-88

12. Alini Marzuki, Abdul Mu’izz Mohamed, Aishah Adam, Yasmin Anum Mohd Yusof, Low Kiat Cheong, & Wan

Zurinah Wan Ngah. Pembiakan mencit Ts65Dn. Prosiding Seminar IRPA RMK-8 2004; 2:94-97 13. Suzana Makpol, Azrina Zainal Abidin, Musalmah Mazlan, Gapor Md. Top & Wan Zurinah Wan Ngah. Kesan γ-

tokotrienol terhadap tekanan oksidatif aruhan H2O2 dalam sel selanjar fibroblas kulit normal. Prosiding Seminar IRPA RMK-8 2004; 2: 98-103

14. Suzana Makpol, Zainie Aboo Bakkar, Musalmah Mazlan, Gapor Md. Top & Wan Zurinah Wan Ngah. Kesan

hidrogen peroksid dan γ-tokotrienol ke atas proliferasi limfosit manusia semasa penuaan. Prosiding Seminar IRPA RMK-8 2004;2: 104-109

15. Then Sue Mian, Musalmah Mazlan, Gapor Mat Top & Wan Zurinah Wan Ngah. Kesan neuroprotektif γ-

tokotrienol dan α-tokoferol terhadap tekanan oksidatif aruhan H2O2 pada kultur sel sistem saraf pusat yang berbeza. Prosiding Seminar IRPA RMK-8 2004; 2: 110-113

16. Nor Isnida I, Musalmah M, Gapor MT & Wan Ngah WZ. Kesan γ-tokotrienol ke atas kerosakan DNA pada

individu normal dan Sindrom Down. Prosiding Seminar IRPA RMK-8 2004; 2: 114-117

17. Rehanna M, Musalmah M, Gapor MT & Wan Ngah WZ. Kajian kesan gamma-tokotrienol terhadap pengambilan glukosa oleh sel L6 miotubul. Prosiding Seminar IRPA RMK-8 2004; 2: 118-120

18. Suhaniza Sulaiman, Wan Zurinah Wan Ngah & Yasmin Anum Mohd Yusof. Kesan kemopreventif Chlorella

vulgaris ke atas tikus teraruh kanser hepar. Prosiding Seminar IRPA RMK-8 2004; 2: 121-125

19. Suhana Md. Saad, Yasmin Anum Mohd Yusof, Wan Zurinah Wan Ngah, Asmah Rahmat & Nor Aripin Shamaan. Perbandingan kesan hasilan alga Chlorella Vulgaris tempatan dan dari Jepun ke atas proliferasi sel ampaian hepar normal dan kanser hepar. Prosiding Seminar IRPA RMK-8 2004; 2: 126-129

20. Roslan Harun. Identification and analysis of genes underlying asthma using cDNA microarray, Seminar IRPA

RMK8 Kategori PR/SR/BTK 2004, Prosiding Seminar IRPA RMK-8 2004; 2: 270-274

21. Roslan Harun, Jeenath Banu Jamaludin, Roohaida Othman, Mohd Saifulaman Mohd Said. Identifikasi gen yang terlibat dalam perkembangan CD4+ve T limfosit dalam asma dan atopi dengan menggunakan mikroatur. Seminar IRPA RMK 8 Kategori PR/SR/BTK 2004. Prosiding Seminar IRPA RMK-8 2004; 2: 161-164.

22. Aminuddin Ahmad, Nursuhadah Yusof, Roslan Harun, Ramelah Mohamed. Corak persembahan klinikal dan

penyakit gastroduodenal terhadap jangkitan Helicobacter pylori serta hubungkait dengan immuniti perumah dan faktor kevirulenan bakteria. Seminar IRPA RMK8 Kategori PR/SR/BTK 2004, Prosiding Seminar IRPA RMK-8 2004; 2: 133-135

23. Munirah S., Aminuddin B.S., Chua K.H., Fuzina N.H., Isa M.R., Izuddin Fahmy A, Azmi B., Badrul A.H.M.Y.,

Saim L., Ruszymah B.H.I. Evaluation of tissue-engineered human articular neocartilage by immunohistochemistry (IHC) technique. 2nd Life Sciences Post-graduate Conference 2004;196-200 [Best Poster Award]



24. Norazlinda MD, Zakinah Y, Farah Wahida I, Lokman BS, Aminuddin BS and Ruszymah BHI. Connexin-26

compound heterozygous mutation results in deafness. 2nd Life Sciences Post-graduate Conference 2004; 285-8

Abstracts in conferences, seminars, workshops

1. Fazlina N, Hamidah NH, Maha A, Zarina AL, Cheong SK, Ainoon O, Leong CF, Jamal R. Multidrug resistance

(MDR) proteins and in vitro cytotoxicity of anti-leukaemic drugs. 5th Malaysian National Haematology Scientific Meeting. 9 -11 April 2004, Marriott Putrajaya, Malaysia, First prize.

2. Zulhabri O, Wan Ngah WZ, Sagap I, Sukumar M, Melati K, Isa MR, Jamal AR. Telomerase activity in human

colorectal cancer. Annual Scientific Meeting of Gastroenterology and Hepatology. 24-27 June 2004, Shangri La Rasa Sayang Hotel, Penang.

3. Fazlina N, Hamidah NH, Maha A, Zarina AL, Cheong SK, Ainoon O, Hamidah A, Zulkifli SZ, Jamal R. Protein

rintang pelbagai dadah (MDR) dan sitotoksisiti dadah anti-leukemia secara in vitro. Ekspo Penyelidikan & Inovasi UKM 2004, 15 –18 July 2004, Dewan Canselor Tun Abdul Razak, UKM, Malaysia, Gold medal.

4. Ramelah M, Alfizah H, Aminuddin A, Isa MR, Nadesan S, Jamal R, Sheikh Anwar A, Rizal AM, Ali Yaakub J.

Helicobacter pylori cagA and vacA genes variants among strains isolated from multiracial patients in Malaysia. Ekspo Penyelidikan & Inovasi UKM 2004, 15 - 18 July 2004, Dewan Canselor Tun Abdul Razak, UKM, Malaysia (Award: Gold Medal for Scientific Poster Competition – Medical Category).

5. Zulhabri O, Wan Ngah WZ, Sagap I, Sukumar M, Isa MR, Azian MD, Melati K, and Jamal AR. Pengesanan

mutasi ‘Hotspot’ gen K-ras2 pada kanser kolorektal. Ekspo Penyelidikan & Inovasi UKM 2004, 15-18 July 2004, Dewan Canselor Tun Abdul Razak, UKM, Malaysia (Award: Silver Medal for Scientific Poster Competition – Medical Category).

6. Fazlina N, Hamidah NH, Maha A, Zarina AL, Cheong SK, Ainoon O, Hamidah A, Zulkifli SZ, Jamal R. Multidrug resistance (MDR) proteins and in vitro cytotoxicity of anti-leukaemic drugs. Minggu Penyelidikan ke-VI Fakulti Perubatan UKM 19 - 24 July 2004, Kompleks Perubatan Tuanku Jaafar HUKM Cheras. First prize & Overall Best Poster.

7. Zulhabri O, Wan Ngah WZ, Sagap I, Sukumar M, Isa MR, Azian M, Melati K, and Jamal AR. The hotspot

mutations of K-ras2 gene in human colorectal cancer. Minggu Penyelidikan Ke-VI, Fakulti Perubatan UKM. 19-24 July 2004, Kompleks Perubatan Tuanku Jaafar HUKM Cheras.

8. Azian Murad, Zuraini R., Melati K., Zulhabri O., Sagap I., Sukumar N., Isa M.R., Jamal R. Use of denaturing

gradient high performance liquid chromatography (DHPLC) for mutational analysis of patients with colorectal cancer. Minggu Penyelidikan Ke-VI, Fakulti Perubatan UKM;19-24 July 2004 & Ekspo Penyelidikan dan Inovasi UKM 2004 15-18 July 2004, Dewan Canselor Tun Abdul Razak, UKM, Malaysia.

9. Fazlina N, Hamidah NH, Maha A, Zarina AL, Cheong SK, Ainoon O, Hamidah A, Zulkifli SZ, Jamal R. Multidrug

resistance (MDR) proteins and in vitro cytotoxicity of anti-leukaemic drugs. Bioteknology Asia 2004 9-11 September 2004, Putra World Trade Centre, Kuala Lumpur, Malaysia, Bronze medal.

10. Jamal R, Fazlina N, Maha A, Zarina AL, Hamidah A, Zulkifli SZ, Cheong SK, Ainoon O, Hamidah NH. Multidrug

resistance (MDR) proteins in acute leukemia cases in a typical hematology - oncology unit in Malaysia. SIOP 2004 36th Congress of The International Society Of Paediatric Oncology 16 – 19 September 2004, Oslo, Norway.

11. Azni AM, Noor A’shikin A, Ridhwan AW, Haslizai H, Lalitha P, Asma I, Mohd Zaki S. Development of Dot-EIA test using recombinant protein specific for Campylobacter jejuni. National Diagnostic Conference and Workshop; 4-7 October 2004; USM/Renaissance Hotel, Kelantan

12. Nik Noorul Shakira MS, Kirnpal-Kaur BS, Ravichandran M, Che Nur Anini, Haslizai H, Asma I, Mohd Zaki S.

Development of a DNA amplification-based assay for the detection of Shigella sonnei. National Diagnostic Conference and Workshop; 4-7 October 2004; USM/Renaissance Hotel, Kelantan

13. Norzie Azlin P, Asma I, Ridhwan AW, Azni A, Ravichandran M, Lalitha P, Zainul FZ, Haslizai H, Mohd Zaki S.

Detection of Campylobacter jejuni by a two-step amplification method. National Diagnostic Conference and Workshop; 4-7 October 2004; USM/Renaissance Hotel, Kelantan



14. Kirnpal-Kaur BS, Asma I, Ravichandran M, Mohd Zaki Salleh. Application of recombinant OmpA protein in the

development of a rapid immunodiagnosis of Shigella flexneri. National Diagnostic Conference and Workshop; 4-7 October 2004; USM/Renaissance Hotel, Kelantan

15. Zulhabri O, Wan Ngah WZ, Sagap I, Sukumar M, Isa MR, Azian M, Melati K & A Rahman AJ. Telomerase

activity in human colorectal cancer. Minggu Penyelidikan ke-VI, 19-24 Julai 2004, Kuala Lumpur 16. Zulhabri O, Wan Ngah WZ, Sagap I, Sukumar M, Isa MR, Azian M, Melati K & A Rahman AJ. The hotspot

mutations of k-ras2 gene in human colorectal cancer. Minggu Penyelidikan ke-VI, 19-24 Julai 2004 Kuala Lumpur

17. Noor Aini AH, Norul Aini Z, Norhani M, Bariah MA, Zainora M, Norlaila MD, Abdul Gapor MT & Wan Ngah WZ

The relationship between lipid peroxidation and induction of cataract, effect of palm vitee. Minggu Penyelidikan ke-VI, 19-24 Julai 2004 Kuala Lumpur

19. Wan Zurinah Wan Ngah - Plenary. Palm Oil Vitamin E: Beyond its antioxidant properties 29th Annual

Conference of the Malaysian Society for Biochemistry and Molecular Biology 28-29 Sept 2004 Kuala Lumpur 20. Then Sue Mian, Musalmah Mazlan, Coral Sanfeliu, Gapor Mat Top and Wan Zurinah Wan Ngah.

Neuroprotective effects of -tocotrienol and -tocopherol against h2o2-induced oxidative stress in different central nervous system (CNS) culture. 29th Annual Conference of the Malaysian Society for Biochemistry and Molecular Biology 28-29 Sept 2004 Kuala Lumpur

21. Goon JA, Noor Aini AH, Aminuddin AHK, Azian AL, Nabisyah M, Zaiton Z, Jamilah B, Rosyida H, Musalmah M &

Wan Zurinah WN. Effects of exercise (tai chi) on oxidative status among population in aging. 29th Annual Conference of the Malaysian Society for Biochemistry and Molecular Biology 28-29 Sept 2004 Kuala Lumpur

22. Harliansyah, Noor Aziaan Murad1, Wan Zurinah Wan Ngah & Yasmin Anum Mohd Yusof. Effects of Zingiber

officinale on proliferation and apoptosis of hepatoma, HEPG2 cell line. 29th Annual Conference of the Malaysian Society for Biochemistry and Molecular Biology 28-29 Sept 2004 Kuala Lumpur

23. Suhaniza Sulaiman, Wan Zurinah Wan Ngah & Yasmin Anum Mohd Yusof. Chemopreventive effect of

Chlorella vulgaris on liver cancer induced rats. 29th Annual Conference of the Malaysian Society for Biochemistry and Molecular Biology 28-29 Sept 2004 Kuala Lumpur

24. Suhana Md. Saad, Yasmin Anum Mohd Yusof, Wan Zurinah Wan Ngah, Asmah Rahmat, & Nor Aripin

Shamman. Comparison between locally produced Chlorella vulgaris and Chlorella vulgaris from Japan on proliferation of normal and liver cancer cell lines. 29th Annual Conference of the Malaysian Society for Biochemistry and Molecular Biology 28-29 Sept 2004 Kuala Lumpur

25. Nafeesa Najwa M.K., Noor Aini A.H, Aminuddin A.H.K, Azian A.L., Nabisyah M, Zaiton Z, Jamilah B, Rosyida H,

Musalmah M & Wan Zurinah W.N. Effects of Tai Chi on immune response among population in retirement age. 29th Annual Conference of the Malaysian Society for Biochemistry and Molecular Biology 28-29 Sept 2004 Kuala Lumpur

26. Suzana Makpol, Mastura Abd. Aziz, Gapor Md. Top & Wan Zurinah Wan Ngah. Effects of -tocotrienol and -

tocopherol on arachidonic acid-induced cytotoxicity and apoptosis in Hep G2 cells transfected with CYP2E1 gene. 29th Annual Conference of the Malaysian Society for Biochemistry and Molecular Biology 28-29 Sept 2004 Kuala Lumpur

27. Looi Mee Lee, Ahmad Zailani hatta Mohd Dali, Siti Aishah Md Ali, Wan Zurinah Wan Ngah and Yasmin Anum

Mohd Yusof. Oxidative status in cervical cancer patients attending O&G clinic at Hospital Universiti Kebangsaan Malaysia. 29th Annual Conference of the Malaysian Society for Biochemistry and Molecular Biology 28-29 Sept 2004 Kuala Lumpur

28. Zakiah J, Azlan A.L, Abdul Gapor M.T & Alini M., Wan Ngah W,Z. Effect of cigarette smoke inhalation on

antioxidant enzymes and lipid peroxidation in mice supplemented vitamin E. 29th Annual Conference of the Malaysian Society for Biochemistry and Molecular Biology 28-29 Sept 2004 Kuala Lumpur

29. Suhana Mamat, Suzana Makpol, Zalinah Ahmad, Gapor Md. Top & Wan Zurinah Wan Ngah. Palm oil vitamin

E protects against lipid peroxidation in Hep G2 cell line expressing cytochrome p450 2e1 enzyme. 29th Annual Conference of the Malaysian Society for Biochemistry and Molecular Biology 28-29 Sept 2004 Kuala Lumpur



30. Azrina Zainal Abidin, Suzana Makpol, Musalmah Mazlan, Gapor Md. Top & Wan Zuriah Wan Ngah. Effect of -

tocotrienol and hydrogen peroxide-induced oxidative stress in normal human skin fibroblast cell lines derived from different age groups. 29th Annual Conference of the Malaysian Society for Biochemistry and Molecular Biology 28-29 Sept 2004 Kuala Lumpur

31. Zaini Aboo Bakar, Suzana Makpol, Musalmah Mazlan,1Gapor Md. Top, & Wan Zurinah Wan Ngah. Effect of -

tocotrienol and hydrogen peroxide-induced oxidative stress in human lymphocytes. 29th Annual Conference of the Malaysian Society for Biochemistry and Molecular Biology 28-29 Sept 2004 Kuala Lumpur

32. Nor Isnida I, Musalmah Mazlan, Gapor MT & Wan Ngah WZ. Effect of gamma-tocotrienol on DNA damage by

comet assay. 29th Annual Conference of the Malaysian Society for Biochemistry and Molecular Biology 28-29 Sept 2004 Kuala Lumpur

33. Rehana M, Musalmah M, Gapr MT & Wan Zurinah W.N. Effect of gamma-tocotrienol on glucose uptake in l6

myotube cell cultures. 29th Annual Conference of the Malaysian Society for Biochemistry and Molecular Biology 28-29 Sept 2004 Kuala Lumpur

34. Normiza R, Yusfairuz M.P, Azlan A.L., Noor Laili M.T., Noor Aini A.H, Norhana Y, Fatimah M, Yasmin A,

Musalmah M, Zaiton Z, Aminuddin A.K, Nabishah M, Jamilah B, Rosyida H, Ibrahim M.J, Lim L.Y, Chin S.F & Wan Ngah W.Z. The determinaton of liver function test, renal profile, lipid profile and full blood count in the aging process study of citizens in Malaysia. 29th Annual Conference of the Malaysian Society for Biochemistry and Molecular Biology 28-29 Sept 2004 Kuala Lumpur

35. Yusfairuz M.P., Normiza R, Azlan A.L, Noor Laili M.T, Noor Aini A.H, Norhana Y, Fatimah M, Yasmin A,

Musalmah M, Zaiton Z, Aminuddin A.K., Nabishah M, Jamilah B, Rosyida H, Ibrahim m.J., Lim L.Y., Chin S.F & Wan Zurinah W.Z. Differences in association of body mass index (BMI) and age with lipid profile in subjects from two geographical locations in Malaysia. 29th Annual Conference of the Malaysian Society for Biochemistry and Molecular Biology 28-29 Sept 2004 Kuala Lumpur

36. Ibrahim M.J., Noor Aini A.H., Noorlaili M.T, Azian A.L, Shahnaz M, Jamilah B, Rosyida H, Zaiton Z, Aminuddin

A.K, Yasmin A., Musalmah M & Wan Ngah W.Z. Effect of Tri e® supplementation on biochemical blood profiles and antioxidant enzymes; a preliminary report. 29th Annual Conference of the Malaysian Society for Biochemistry and Molecular Biology 28-29 Sept 2004 Kuala Lumpur

37. Lim, L.C., Azlan A.L. 1Nor Aini A.H., Musalmah M., Yasmin A., Zaiton Z.2, Nabishah M.2, Aminudin A.K.2,

Normiza R., Yusfairuz M.P., Noor Laili M.T.3, Rosyida H.4, Jamilah B.5, Norhana Y.6, Fatimah M.6 & Wan Ngah W.Z. The effect of age on catalase and superoxide dismutase enzymes activities among Malaysians. 29th Annual Conference of the Malaysian Society for Biochemistry and Molecular Biology 28-29 Sept 2004 Kuala Lumpur

38. Carolone Suggip, Maziana Mahamood, Laila Mehat, Mizaton Hazizul Hasan, Noor Aisha Abdullah, Alili Marzuki,

Wan Zurinah Wan Ngah, Noor Aini Abd. Hamid & Aishah Adam. Plasma tocopherol and tocotrienol in trisomy 21 persons were not different from those of non-trisomy 21 persons. 29th Annual Conference of the Malaysian Society for Biochemistry and Molecular Biology 28-29 Sept 2004 Kuala Lumpur

39. Chin S F, Azian A L, Noor Aini A H, Norlaili T, Zaiton Z, Aminuddin A K, Nabishah M, Yasmin A, Musalmah M,

Abahim M J, Shahnaz M, Jamilah B, Rosyida H & Wan Ngah W Z. The effect of age, DNA damage and lipid profile in the population: A preliminary report. 29th Annual Conference of the Malaysian Society for Biochemistry and Molecular Biology 28-29 Sept 2004 Kuala Lumpur

40. Khalilah Sairin, Suzana Makpol, Musalmah Mazlan, Abdul Gapor Md Top and Wan Zurinah Wan Ngah Effect of

gamma-tocotrienol on the viability of normal human young skin fibroblast cell line. 29th Annual Conference of the Malaysian Society for Biochemistry and Molecular Biology 28-29 Sept 2004 Kuala Lumpur

41. Rosmadi Mohd Yusof, Mohd Saifulaman Mohd Said, Ahmad Faizal Mohd Perdaus, Roslina Abdul Manap,

Roslan Harun. 2004. Gene expression profiling of lung adenocarcinoma by microarray analysis. Minggu Penyelidikan Ke VI Fakulti Perubatan UKM

42. Jeenath Banu Jamaluddin, Roslan Harun.2004. Pengenalpastian pengekspresan gen pembezaan dalam darah

korda limfosit T pembantu CD4+ve dalam atopik asma. Minggu Penyelidikan Ke VI Fakulti Perubatan UKM. 43. Nur Amani Aris, Roslan Harun, Ahmad Faizal Mohd Perdaus. 2004. Complication of flexible bronchoscopy, the

effects of endobronchial biopsy: A one month observational study. Minggu Penyelidikan Ke VI Fakulti Perubatan UKM.



44. Ahmad Faizal Mohd Perdaus, Abdul Rahim Noah, Abdul Latiff Mohd, Jeffrey Abu Hassan, Roslina Abdul Manap,

Roslan Harun. 2004. Neutrophil Function and Serum Interleukin 8 in chronic stable bronchiectasis. Minggu Penyelidikan Ke VI Fakulti Perubatan UKM.

45. Rosmadi Mohd Yusof, Roslan Harun, Mohd Saifulaman Mohd Said. 2004, Microfluidic and HPLC analysis of

novel lung cancer genes identified by arbitrarily primed PCR. Minggu Penyelidikan Ke VI Fakulti Perubatan UKM

46. Mohamed Saifulaman Mohamed Said, Rosmadi Mohd Yusoff, Roslan Harun. 2004. Gene profiling of lung

adenocarcinoma by microarray analysis in Malaysian patients. HUGO Human Genome Meeting 2004, Berlin, Germany

AWARDS WON IN 2004 BY MEMBERS OF UMBI Pingat Kebesaran Ahli Setia Negeri Sembilan (ANS), 12 October 2004, Istana Kenangan Seri Menanti, Negeri Sembilan

Professor Dr. A Rahman A Jamal ITEX, Geneva Gold medal

Bioengineered human skin using HPD. Ruszymah Hj. Idrus Ministry of Science & Innovation Award Gold medal

UKM-MECC : The optimized medium for human cartilage tissue engineering. Ruzymah Hj. Idrus et al

5th Malaysian National Haematology Scientific Meeting. 9 - 11 April 2004, Marriott Putrajaya, Malaysia First prize

Multidrug resistance (MDR) proteins and in vitro cytotoxicity of anti-leukaemic drugs. Fazlina N, Hamidah NH, Maha A, Zarina AL, Cheong SK, Ainoon O, Leong CF, Jamal R.

UKM Research & Innovation Expo, 15-18 July 2004, Dewan Canselor Tun Abdul Razak, UKM Gold medal

Hamidah NH, Fazlina N, , Maha A, Zarina AL, Cheong SK, Ainoon O, Hamidah A, Zulkifli SZ, Jamal R. Protein rintang pelbagai dadah (MDR) dan sitotoksisiti dadah anti-leukemia secara in vitro.

Silver medal

Zulhabri O, Wan Ngah WZ, Sagap I, Sukumar M, Isa MR, Azian MD, Melati K, and Jamal R. Pengesanan Mutasi

‘Hotspot’ Gen K-ras2 pada Kanser Kolorektal.

Ramelah Mohamed. Pembangunan assai PCR multipleks dan pengesanan mutasi gen toksin pada Vibrio cholerae 01

4th Medical Faculty UKM Research Week , 19 - 24 July 2004, Tuanku Jaafar Medical Complex, HUKM Cheras First prize & Overall Best Poster

Fazlina N, Hamidah NH, Maha A, Zarina AL, Cheong SK, Ainoon O, Hamidah A, Zulkifli SZ, Jamal R. Multidrug resistance (MDR) proteins and in vitro cytotoxicity of anti-leukaemic drugs



Biotechnology Asia 2004, 9-11 September 2004, Putra World Trade Centre, Kuala Lumpur, Malaysia Bronze medal

Fazlina N, Hamidah NH, Maha A, Zarina AL, Cheong SK, Ainoon O, Hamidah A, Zulkifli SZ, Jamal R. Multidrug resistance (MDR) proteins and in vitro cytotoxicity of anti-leukaemic drugs.

2nd Life Sciences Post-graduate Conference Best Poster

Munirah S., Aminuddin B.S., Chua K.H., Fuzina N.H., Isa M.R., Izuddin Fahmy A, Azmi B., Badrul A.H.M.Y., Saim L., Ruszymah B.H.I. Evaluation of tissue-engineered human articular neocartilage by imunohistochemistry (IHC) technique.

UKM’s Best Researcher Award

Associate Professor Dr. Ruszymah Hj. Idrus

UKM’s Excellent Service Award

Associate Professor Dr. Roslan Harun Ms Bahiyah Tuah



NETWORKING & COLLABORATION UMBI has established networking and collaboration with several international and local research institutions and industries as listed below. We will embark on establishing a wider network with other institutions in the coming years. International institutions

King’s College Medical School, London, United Kingdom Fred Hutchinson Cancer Research Centre, Seattle, Washington, USA University of South Australia Kyorin University School of Medicine, Tokyo, Japan Graduate School of Medicine, University of Kyoto, Japan

Local institutions

Institute for Research In Molecular Medicine (INFORMM), Universiti Sains Malaysia Institute of Biotechnology, Universiti Teknologi Mara Faculty of Pharmacy, Universiti Teknologi Mara School of Medical Sciences, Universiti Sains Malaysia INNOBIOLOGICS Faculty of Medicine and Health Sciences, Universiti Putra Malaysia Institute Medical Research Hospital Kuala Lumpur

Industries

BioSynTech Sdn. Bhd. Aventis Pharma Siber Hagner Chemoscience Research Instrument



ACKNOWLEDGEMENTS

Vice Chancellor, UKM

Deputy Vice Chancellor (Academic and International

Affairs), UKM

Deputy Vice Chancellor (Research and Innovation Affairs),

UKM

Deputy Vice Chancellor (Student and Alumni Affairs), UKM

Dean, Faculty of Medicine, UKM

Director, Hospital Universiti Kebangsaan Malaysia, UKM

Director, Research Management Centre, UKM

Registrar, UKM

Bursar, UKM



Documents

1 Cover LT 2004 - Official Portal of UKM Report... · 2014-12-04 · Professor Dr. Ramelah Mohamed Suhaili Abu Bakar Principal Fellow VK7 Scientific Officer C41 (IRPA) [email protected]