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Parameter to differentiate IDA & ACD FEP

Decreased in IDA

Increased in ACD

Hb SC Hb S, hb C, hb F

Sero test for german measles IgG

IgM

Latex agglutination

Complement fixation

MDS FAB

RA

RARS

RAEB

CMMOL

RAEBT

MDS WHO

RA/RCUD/RN/RT

RARS

RAEBT 1

RAEBT 2

MDSNU

MDS DEL 5Q

Indention stage start Metamyleocyte

Hb production begins Prorubricyte

Pink hb, blue rna, polychromatic erythroblast Rubricyte

Overall blue, polychromatophilia

Polychromatic red cell

Reticulocyte

Meg csf BM

In reponse to megakaryocyte mass in BM

Thrombopoietin Kidney, liver, spleen

In response to platelet demand in circulation

Haptoglobin Intravascular hemolysis

Extravascular

Variant lymphocyte plasmacytoid L Type 1

T cell differentiation Thymus

FAB cell line Cell morph = romanowsky

Differentiation = cytochemical

WHO Immunophenotyping = cell line

Cytogenetic = classify leukemia

Hemoglobin 4 heme, 4 globin

Sickle cell Decreased OFT

Resistant to malaria

spherocytosis Increased OFT

Increased autohemolysis

Major protein Spectrin

SEA ovalocytosis / stomatoctosis HE Resistant to malaria

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Embden meyerhoff PK def

Pentose phosphate G6PD def

Hereditary stomatocytosis/xerocytosis Autohemolysis test : not corrected

Hereditary pyropikilocytosis Increased autohemolysis test

G6PD def Lyons hypothesis, male is resistant to malaria

Oslers disease Polycytemia vera

Pancytosis, panmyelosis

Normo normo, rpi>3 a) PRCA = N/N, rpi3

d) AOTA

Vcs tech, wbc differential Coulter LH750

Size in rbc MCV

FAB ALL L1

Small, homogenous, children, PAS

L2

Large, heterogenous, adult, similar to aml ml2

L3

Large, homogenous, adult, children, burkitt, oil red

Hairy cell Pancytopenia, fried egg, trap,

Tell LGLL RA, FELTY SYNDROME

REED STERNBERG CELL Hodgkin lymphoma, giant cell, owl eye,

Lacunar cell Tumor cell, cytoplasmic clearing

Popcorn cell Multilobed nucleus, indistinct nucleoli

Sezary cell Cutaneous T lymphoma

Mycosis fungioides Sezary cell

Cerebriform nucleus

Patutrier microabscess

HAM acidified serum test PNH confirmation

(+) secondary aplastic anemia

Sugar water screening test 9-10% sucrose, hemolysis (+)

CD 45 Highest in

Lowest in

Embrionic Mesoblastic stage

Gower 1, portland, gower 2

Weak acetic acid dilute Rbc

Correct for nrbc & wbc Cell dyn abott

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Basket cell 22% albumin, before smear, for leukemic,destroyed neutrophil

Smudge cell 22% albumin,

Dense granules Serotonin, calcium , adp, atp

PTP 1week after platelet transfusion

Anti HPA1a

Bleeding 2-12 days after transfusion

NAIT ALLOIMMUNE

anti HPA1a

NAT Mom, SLE

decreased platelets in babies

Stormorken syndrome Platelet express phosphatidylserine without prior stim

ulation

Scott syndrome Abnormal calcium

Phospholipid scramblinh

Platelet cannot undergo flip flop effect

Cannot bind coagulation factor

Beta thromboglobulin

Pf4

Thrombospondin