© University of Sydney© University of Sydney

AustralianCerealRustControlProgram

Professor Robert F. Parkrobert.park@sydney.edu.au

Australian Cereal Rust Control ProgramConsultative CommitteeDAFWA, Perth

March 21st 2013

Director’s Report

© University of Sydney© University of Sydney

WA residents told to prepare for Cyclone Rusty!

Monday 25th February 2013, AEST 9.00 am

http://www.abc.net.au/news/2013-02-25/wa-residents-told-to-prepare-for-cyclone-rusty/4537056

© University of Sydney

Scientific staff:Urmil Bansal

Harbans Bariana

Robert Park

Peng Zhang

Davinder Singh

Haydar Karaoglu

Peter Dracatos

Honorary Associates:William Cuddy

Bob McIntosh

Ray Hare

Colin Wellings

General staff:Muhammed Gill

Sami Hoxa

Keshab Kandel

Hanif Miah

Gary Standen

Matthew Williams

Personnel, as @ March 2013

Postgraduate students:Jordan Bailey

Mumta Chettri

Lida Derevnina

Banchgize Temesgen

Leila Kamino

Tolera Keno

Mandeep Randhawa

Huda Elmansour

Peace Kankwatsa

Vallence Nsabesyera

Mesfin Gesesse

Bosco Chemeyek

Ali Salman

© University of Sydney

Personnel changes during 2012

Four technical positions made redundant:

- Mr Gary Standen – Fee for Service, GRDC funded.- Mr Paul Kavanagh – Non-wheat rust surveys and genetics- Ms Kanwal Kaur – Greenhouse testing (FFS, Detailed tests)- Mr Keshab Kandel – Stripe rust surveys and genetics

Dr Wellings retired, June 2012

Park Appointment

New Appointments:

- Mr Gary Standen appointed, FFS (non-GRDC funds)- Mr Keshab Kandel appointed, Rust surveys- Dr Will Cuddy appointed, NSWDPI, Plant Pathologist Rust Diseases- Business Manager TBA-HEO 3 Technician TBA

© University of Sydney

Personnel changes during 2012

Four technical positions made redundant:

- Mr Gary Standen – Fee for Service, GRDC funded.- Mr Paul Kavanagh – Non-wheat rust surveys and genetics- Ms Kanwal Kaur – Greenhouse testing (FFS, Detailed tests)- Mr Keshab Kandel – Stripe rust surveys and genetics

Dr Wellings retired, June 2012

Park Appointment

New Appointments:

- Mr Gary Standen appointed, FFS (non-GRDC funds)- Mr Keshab Kandel appointed, Rust surveys- Dr Will Cuddy appointed, NSWDPI, Plant Pathologist Rust Diseases- Business Manager TBA-HEO 3 Technician TBA

© University of Sydney

Personnel changes during 2012

Four technical positions made redundant:

- Mr Gary Standen – Fee for Service, GRDC funded.- Mr Paul Kavanagh – Non-wheat rust surveys and genetics- Ms Kanwal Kaur – Greenhouse testing (FFS, Detailed tests)- Mr Keshab Kandel – Stripe rust surveys and genetics

Dr Wellings retired, June 2012

New Appointments:

- Mr Gary Standen appointed, FFS (non-GRDC funds)- Mr Keshab Kandel appointed, Rust surveys- Dr Will Cuddy appointed, NSWDPI, Plant Pathologist Rust Diseases- Business Manager TBA- HEO 3 Technician TBA

© University of Sydney

Previously, ACRCP was 1 project – now 4 projects: 

1. ACRCP - National breeding support. US00064

2. ACRCP - Durable genes. US00063

3. ACRCP - Towards 2019 and a century of monitoring cereal rust pathogens in Australia. US00067

4. ACRCP- Molecular marker program CSIRO/Univ. Sydney/CIMMYT collaborative project. CSP00164

Current status of “new” projects [1 July 2012 thru 30 June 2017]

Proposals include:

- reducing Director’s salary to zero, incrementally, over project life- GRDC to fund (50%) Business Manager- Fee for Service subsidy to reduce to zero (wheat by year 3, barley by year 4)- full review of FFS, including costings, once Business Manager is appointed - establishment of a Breeder’s Advisory Group

© University of Sydney

Primary purpose is to give effect to the Australia-US Defence Trade Cooperation Treaty, signed by the Bush and Howard governments in 2007

Main benefit is to provide some Australian defence industries with relief from a restrictive export control regime that has hampered Australian-US defence goods and defence industry trade

The Bill has established a potentially stifling export control regime for “intangible transfers” of technology that will impact a broad range of what would ordinarily be open research in Australian universities etc

Dept of Defence has been determined to meet the US imposed conditions for treaty ratification by implementing an incredibly restrictive regulatory regime - a permit regime, for intangible transfers of knowledge in relation to all items listed on the Defence Strategic Goods List (380 page document with a 79 page index….)

Late in the development of the Bill, Defence put forward an amendment making publication in contravention of the permit regime a crime, with a penalty of up to 10 years in jail

Puccinia graminis f. sp. tritici and P. striiformis f. sp. tritici appear on the DGSL.

Defence Trade Controls Act 2012

© University of Sydney

Primary purpose is to give effect to the Australia-US Defence Trade Cooperation Treaty, signed by the Bush and Howard governments in 2007

Main benefit is to provide some Australian defence industries with relief from a restrictive export control regime that has hampered Australian-US defence goods and defence industry trade

The Bill has established a potentially stifling export control regime for “intangible transfers” of technology that will impact a broad range of what would ordinarily be open research in Australian universities etc

Dept of Defence has been determined to meet the US imposed conditions for treaty ratification by implementing an incredibly restrictive regulatory regime - a permit regime, for intangible transfers of knowledge in relation to all items listed on the Defence Strategic Goods List (380 page document with a 79 page index….)

Late in the development of the Bill, Defence put forward an amendment making publication in contravention of the permit regime a crime, with a penalty of up to 10 years in jail

Puccinia graminis f. sp. tritici and P. striiformis f. sp. tritici appear on the DGSL.

Defence Trade Controls Act 2012

© University of Sydney

Primary purpose is to give effect to the Australia-US Defence Trade Cooperation Treaty, signed by the Bush and Howard governments in 2007

Main benefit is to provide some Australian defence industries with relief from a restrictive export control regime that has hampered Australian-US defence goods and defence industry trade

The Bill has established a potentially stifling export control regime for “intangible transfers” of technology that will impact a broad range of what would ordinarily be open research in Australian universities etc

Dept of Defence has been determined to meet the US imposed conditions for treaty ratification by implementing an incredibly restrictive regulatory regime - a permit regime, for intangible transfers of knowledge in relation to all items listed on the Defence Strategic Goods List (380 page document with a 79 page index….)

Late in the development of the Bill, Defence put forward an amendment making publication in contravention of the permit regime a crime, with a penalty of up to 10 years in jail

Puccinia graminis f. sp. tritici and P. striiformis f. sp. tritici appear on the DGSL.

Defence Trade Controls Act 2012

© University of Sydney

Primary purpose is to give effect to the Australia-US Defence Trade Cooperation Treaty, signed by the Bush and Howard governments in 2007

Main benefit is to provide some Australian defence industries with relief from a restrictive export control regime that has hampered Australian-US defence goods and defence industry trade

The Bill has established a potentially stifling export control regime for “intangible transfers” of technology that will impact a broad range of what would ordinarily be open research in Australian universities etc

Dept of Defence has been determined to meet the US imposed conditions for treaty ratification by implementing an incredibly restrictive regulatory regime - a permit regime, for intangible transfers of knowledge in relation to all items listed on the Defence Strategic Goods List (380 page document with a 79 page index….)

Late in the development of the Bill, Defence put forward an amendment making publication in contravention of the permit regime a crime, with a penalty of up to 10 years in jail

Puccinia graminis f. sp. tritici and P. striiformis f. sp. tritici appear on the DGSL.

Defence Trade Controls Act 2012

© University of Sydney

Camden Campus - Centre for Carbon, Food & Water

© University of Sydney

Camden Campus – CER complex

© University of Sydney

PBI Infrastructure – greenhouse polycarbonate

© University of Sydney

Fee for Service, wheat test numbers 2007 through 2012

 Wheat: Year

Test: 2007 2008 2009 2010 2011 2012

Field tests 121,082 80,642 100,374 96,811 60,338 45,076

Greenhouse tests 41,626 15,903 8,287 21,999 33,201 22,509

F2 Long Rows 217 295 277 944 611 199

 Non-wheat: Year

Test: 2007 2008 2009 2010 2011 2012

Field tests 10,628 12,210 13,142 11,617 9,885 13,128

Greenhouse tests 12,518 13,726 15,021 17,327 24,993 30,128

© University of Sydney

Fee for Service, wheat test numbers 2007 through 2012

 Wheat: Year

Test: 2007 2008 2009 2010 2011 2012

Field tests 121,082 80,642 100,374 96,811 60,338 45,076

Greenhouse tests 41,626 15,903 8,287 21,999 33,201 22,509

F2 Long Rows 217 295 277 944 611 199

 Non-wheat: Year

Test: 2007 2008 2009 2010 2011 2012

Field tests 10,628 12,210 13,142 11,617 9,885 13,128

Greenhouse tests 12,518 13,726 15,021 17,327 24,993 30,128

© University of Sydney

Field rust inoculations in 2012- 19 pathotypes across 5 field sites

Crop Rust PathotypeWheat Leaf rust 104-1,2,3,(6),(7),11+Lr37 [=576]    104-1,2,3,(6),(7),11,13 [=547]    10-1,3,7,9,10,12 [=592]    76-1,3,5,7,9,10,12 +Lr37 [=621]  Stem rust 98-1,2,3,5,6,7 [=580]    34-2,12,13 [=427]    34-1,2,7 +Sr38 [=565]    343-1,2,3,4,5,6 [=429]  Stripe rust 134 E16 A+ Yr17+ Yr27+ [=617]    134 E16 A+ J+ T+ [=???]    150 E16 A+ [=598]Barley Leaf rust 5457P+ [=612]  Stripe rust BGYROats Crown rust 4473-4,16,10 +BB [=567]    0107-4,6,10+WN [=595]    0207-1,5,6,10,12+G [=549]    0307-345610+WV [=605]    264 [=164]  Stem rust 41+Pg9,13,a [=512]Triticale Stem rust 34-2,12,13 [=427]  Stripe rust 134 E16 A+ J+ Yr27+    134 E16 A+ J+ T+ [=???]

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Field rust pathotypes – FFS sampling for pathotype ID

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Field sites

Lansdowne 3 Year rotation, 4.5haHorseunit 3 Year rotation, 3.6haBreakwell 3 Year rotation, 1haKaralee 3 Year rotation, 0.7haJ.B. Pye* 3 Year rotation, 0.8haSheep Unit 3 Year rotation, 1.2ha

* Also have a further 7.5ha (2.5ha x 3 year rotation) available from 2013

© University of Sydney

Field – Karalee redevelopment

© University of Sydney

Dam

Roadway

“Cage 2” “Cage 1”

Tunnelhouses (horticulture)

Year 1

Year 2

Year3

X X

Field – Karalee redevelopment

© University of Sydney

Field - proposed restructure, Horse & Sheep Units

Horse Unit

Irrigation line

© University of Sydney

USyd ACRCP Outputs, 5.5 years, 01/07/2007 to 28/02/2013– Outreach and training

Wrote/ distributed/completed:

6 Annual Pathogenicity Survey reports

41 Cereal Rust Updates

75 extension articles for newspapers (incl. Ground Cover) etc

44 media releases including radio interviews.

Contributed to 43 grower/ agronomist workshops/ field days

Participated in 25 national/ international conferences/ meetings

Published 88 peer-reviewed journal articles/ book chapters

Completed 13 PhD and 5 Masters students, in rust pathology and genetics

© University of Sydney

Extension - All Stage / Adult Plant Resistance

© University of Sydney© University of Sydney

© University of Sydney

Genetic basis of disease responseRust resistance genotypes: Cereal Rust Reports

Rust Response Rust Resistance GenotypeLeaf Rust

Stem Rust

Stripe Eastern States

WA WA pt Yr17-27 pt Leaf Rust Stem RustStripe Rust

AGT Katana

MS MR MS-S MR-MS MR-MS Lr13Sr30

AGT Scythe

MS MS R-MR MS-S MS-S Sr13

Amarok R R S R MR-MS Lr14a, Lr37 Sr38 Yr17Annuello R-MR R-MR R-MR MS-S MS-S Lr24, Lr34 Sr24 Yr18Arrino MS MS S-VS S S Sr30 C

Arrivato MR MR R R-MR R-MR Sr8b, Sr9e, Sr13Axe MR MR MS R-MR R-MR Sr8bBabbler MS R R-MR MS-S MS-S Lr24, Lr34 Sr24 Yr18Banks MR MR MR MR-MS MR-MS Lr13 Sr30

Barham MR-MS MR-MS MS R MS-S Lr1, Lr20, Lr34, Lr37

Sr9b,Sr15, Sr38 Yr17, Yr18

© University of Sydney

© University of Sydney

Current research activities – Rust pathogens

DNA marker development – SSRs now available for:- P. graminis - all formae speciales- P. striiformis – all formae speciales- P. triticina- P. hordei

Old “104” “WA” pt. USA BGYR BYR Psd Psp

e.g. P. striiformis [Jordan Bailey]:

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P

Stem rust race Ug99- lecture overview

Pgt sequencing initiative

- have 86 isolates to sequence- sequenced 61 so far, total cost $60k

- dendrogram based on 3.042 million polymorphic SNP loci

© University of Sydney

P

Stem rust race Ug99- lecture overview

Pgt sequencing initiative

- have 86 isolates to sequence- sequenced 61 so far, total cost $60k

- dendrogram based on 3.042 million polymorphic SNP loci

“Ug99” group

© University of Sydney

P

Stem rust race Ug99- lecture overview

Pgt sequencing initiative

- have 86 isolates to sequence- sequenced 61 so far, total cost $60k

- dendrogram based on 3.042 million polymorphic SNP loci

© University of Sydney

P

Stem rust race Ug99- lecture overview

Pgt sequencing initiative

- have 86 isolates to sequence- sequenced 61 so far, total cost $60k

- dendrogram based on 3.042 million polymorphic SNP loci

© University of Sydney

P

Stem rust race Ug99- lecture overview

Pgt sequencing initiative

- have 86 isolates to sequence- sequenced 61 so far, total cost $60k

- dendrogram based on 3.042 million polymorphic SNP loci

- informed selection of isolates for 1st cohort means a huge amount of information will be extracted from this data set

© University of Sydney

Wheat - APR and ASR to stripe rust, leaf rust and stem rust; cytogenetics

Barley - APR and ASR to leaf rust, ASR to stem rust; cytogenetics

Oats - APR and ASR to crown rust and stem rust

Triticale - APR and ASR to stripe rust, leaf rust and stem rust

Non-host / near-host resistance in cereals to rust diseases

Current research activities– Gene discovery & characterisation

© University of Sydney

Backcrossing stripe rust resistance genes into Avocet; 41 gene combination lines produced so far:

Current research activities– Gene discovery & characterisation

Yr1/Yr6Yr1/Yr9Yr1/Yr10Yr1/Yr17Yr1/Yr26Yr1/Yr27Yr1/Yr32Yr1/YrSp

Yr5/Yr26Yr5/Yr27

Yr6/Yr27Yr6/Yr9Yr6/Yr17Yr6/Yr24Yr6/Yr32Yr6/YrSp

Yr7/Yr17Yr7/Yr24Yr7/Yr27Yr7/Yr32

Yr10/Yr15Yr10/Yr24Yr10/Yr26Yr10/Yr27

Yr15/Yr26Yr15/Yr24//Yr10

Yr17/Yr27Yr17/Yr32

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The Rust Bust Campaign

Belinda’s Rust Bust Presentation

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