Slide 1

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IP3, NAADP, cADPr .

5Courtesy of DOE: www.nano.gov/html/facts/The_scale_of_things.html

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10-100

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11

12

Sizing-Down

3m60m .100 m10 m1 m100 nm10 nm1 nm, , , , .13

14 1) . .

2) .

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: (GFP) DNA-GFP Plasmid GFP

RNA-GFP ?2)

: 2+

?? (~15 um ) ,

ExEm (SKBR3) Fura-2AMEx: 340, 380 nmEm: 540 nm

(340/380)

CCD (Roper)(Sutter) (Eppendorf) (Eppendorf)Perfusion System

IP3- Ca+2 LyERIP3Ca2+IP3 inositol triphosphate

Traces = average 6 cells +/- s.e.m

cADPr- Ca+2 LyERcADPrCa2+cADPr - cyclic adenosine diphosphate ribose cADPr . Ry . ER cADPr Ry .Traces = average 6 cells +/- s.e.m

= :

: ( , 25nm) (, 8nm) (10nm)

()

(ER)

: ,

Golgi ER .

, . Ca+2

.

:IP3 Inositol triphosphatecADPr Cyclic adenosine diphosphate riboseNAADP Nicotinic acid adenine dinucleotide phosphate

: (ER) IP3 and cADPr (Ly) NAADP** .

,

150mM K+4mM K+0.1M Ca++2mM Ca++20mM Na+145mM Na+4mM Cl-110mM Cl-IntracellularExtracellular ,

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Plate of cellsCellsIntroduce virusVirusInfected cells37 ()

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Submicron Diameter - Fluid insideCell MembraneINTRACELLULAREXTRACELLULAR - , , , ,

, -

Submicron Diameter :

Cylindrical NanochannelFluid insideCell MembraneINTRACELLULAREXTRACELLULARNanochannel connected to larger probe

PinjectionFlowLDP2P1FlowP1 > P2

(CNTs)

Carbon Nanotubes

Single Wall (SWCNT)Multi Wall(MWCNT)SWCNT 0.6 1.8 nm, 20 nm 500 mm, ,

MWCNT - >0.6 nm, 20 nm o 500+ mm, ,

, MWCNT10 nm (>50nm)

Narishige Piston Injector Eppendorf Femtojet Injector 6000 hPa :

AFM

- . : +

Electrical ConnectionQuartz ExteriorInnerCarbon FilmExposed Carbon Tip1 cm

Carbon TipQuartz Micropipette5 m

.

1mm2 .

20m 100nm 200m : ( )

. .

25 m

Scale = 200 m

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