The Characterizing and Understanding the HIV Reservoir for

TheCharacterizingandUnderstandingtheHIVReservoirforEradication (CURE)cohort:anewresourceforunderstandingHIVpersistence

NatalieN.Kinloch1,2*,AniqaShahid1,2*,BruceGanase1,Hanwei Sudderuddin1,DelphineDuhirwe1,AlineTrevisan1,Zhong Dang1,F.HarrisonOmondi1,2,Hesham Ali3,ChadDickie4,SilviaGuillemi1,MarkHull1,MelKrajden5,ChansonJ.Brumme1,6,Marianne

Harris1,6,MarkA.Brockman1,2,Zabrina L.Brumme1,2(*showsco-firstauthors)

1BritishColumbiaCentreforExcellenceinHIV/AIDS,Vancouver,BritishColumbia;2FacultyofHealthSciences,SimonFraserUniversity,Burnaby,BritishColumbia;3PositiveLivingSocietyofBritishColumbia,Vancouver,BritishColumbia;4AVIHealthandCommunityServices,Victoria,BritishColumbia;5BritishColumbiaCentreforDisease

Control,Vancouver,BritishColumbia;6DepartmentofMedicine,UniversityofBritishColumbia,Vancouver,BritishColumbia

Poster ID BSP6.04

Presentingauthordeclaration:Ihavenoconflictsofinteresttodeclare

Contactusat:ashahid@sfu.ca,zbrumme@sfu.ca

1UNIVERSITYOFBRITISHCOLUMBIA

• ThedevelopmentofeffectiveHIVremissionorcurestrategieswillrequireadeeperunderstandingofinter-individualvariationinHIVreservoirsizeandcomposition.

• Tothisend,wehaveestablishedtheCharacterizingandUnderstandingtheHIVReservoirforEradication (CURE)cohortcomprisingindividualslivingwithHIVonlong-termcART.

Inthisposter,wedescribeCUREandexploreassociationsbetweenclinicalhistoryandlatentHIVreservoirsize.

BACKGROUND

METHODSParticipantrecruitmentand

informedconsentPBMCisolationBlooddraw(450mL) CD4isolationfromPBMCs

GenomicDNAextraction

QuantificationoftotalandintactlatentreservoirusingIntactProviral DNAassayviadropletdigitalPCR(ddPCR)forN=14 CUREparticipants

orescence

KinlochNetal,unpublished

RESULTS

Table1:CharacteristicsoftheCUREcohortParticipantsrecruitedsofar 31

Biological sex

Male 29

Female 2

Medianage(IQR) 57(48-63)years

HIVsubtype B

Median(IQR)estimateddurationofuncontrolledHIVinfection 7(2-15)years

Subsequentmedian(IQR) cART duration 13(9-20)years

Median(IQR)nadirCD4+ T-cellcount 280(120-630)cells/mm3

Median(IQR)archivedpre-cART plasmaavailable 5(2-16)samples/participant

• CUREisanewCanadianHIVcohort,comprisingindividualswithdiverseclinicalhistories,includingthosetreatedinearlyinfectionandlong-termHIVsurvivors,willadvanceourunderstandingofHIVpersistence.

• TheavailabilityofarchivedplasmaspecimenswillallowustoinvestigatethereservoirdiversityinthecontextofHIV'swithin-hostevolutionaryhistory. 5

0 1000 2000 3000 4000 50000

400Spearman's rho = 0.77p-value=0.001

Total HIV reservoir size

ticall

r size

Figure1:LatentHIVreservoirsizeoftheCUREparticipants

RESULTSFigure2:Positivecorrelationbetweentotalandintactreservoir

CONCLUSION

Nosignificantcorrelationsobservedbetweenuncontrolledinfectionduration,cART duration,nadirCD4count,andreservoirsize.

Total reservoir Defective reservoir Genetically intact reservoir0

6001000

(N=14)

The Characterizing and Understanding the HIV Reservoir for

Documents

HIV infection of the CNS: HIV-1 cure Implications for cure · PDF fileImplications for cure ... Brain Lymph nodes Genital tract Gut Is the CNS a viral reservoir? ... Laser capture

Characterizing the Epidemiology of the 2009 Influenza A/H1N1 …chowell.lab.asu.edu/publication_pdfs/Characterizing the... · 2012. 11. 19. · Characterizing the Epidemiology of

Long-term ART initiated during primary HIV-1 infection limits the HIV-1 reservoir size but not to levels found in LTNPs Eva Malatinkova, Ward De Spiegelaere,

EIS 11/2017 Environment Characterizing Characterizing

Towards a therapeutic vaccine to reduce HIV reservoir, both neutralizing HIV and inhibiting effector T cells depletion Pr. Patrice DEBRE Laboratoire Immunité

MEASURING THE LATENT HIV RESERVOIR HIV Cure Research Training Curriculum The HIV CURE training curriculum is a collaborative project aimed at making HIV

PROSPECTS FOR HIV CURE IN ADULTS - Amazon S3 FIS PDF/Monday... · The Problem: Barriers to an HIV Cure HIV infects CD4+ cells. ‘Reservoir’ created Reservoir size impacts clinical

ON CHARACTERIZING THE PHYSICALindividual.utoronto.ca/.../Wilson-On-Characterizing... · characterizing the physical, especially for purposes of formulating physi-calism. I will motivate

BAB II KAJIAN PUSTAKA 2.1. Definisi infeksi HIV · juga dilaporkan sebagai reservoir infeksi laten. Reservoir yang lain meliputi sel mikroglobial dan sel makrofag pada cranial nerve

Characterizing reservoir properties for the lower triassic

Reservoir Simulation-Based Modeling for Characterizing

BEULAH RESERVOIR 2000 RESERVOIR SURVEY - usbr.govusbr.gov/tsc/techreferences/reservoir/Beulah ResSurvey 2000 Reservoir Survey.pdfBEULAH RESERVOIR 2000 RESERVOIR SURVEY by Ronald L

Burrhole Reservoir SPRUNG RESERVOIR

Well test, rate transient analysis and reservoir ... · PDF fileREVIEW PAPER - PRODUCTION ENGINEERING Well test, rate transient analysis and reservoir simulation for characterizing

2014 “Towards an HIV Cure” symposium Melbourne Genetically Characterizing the Role of Cell Proliferation in Maintaining Persistent HIV during Effective

The forces driving clonal expansion of the HIV-1 latent reservoir...HIV-1 integration site-driven clonal expansion leads to a slow but steady increase of HIV-1-infected cells. Y axis,

Advanced seismic techniques help in ARTICLE characterizing ...€¦ · required more advanced seismic attributes to address the issue of reservoir heterogeneity for this thin unconventional

Characterizing and Evaluating Different Deployment ... - Characterizing... · Characterizing and Evaluating Different Deployment ... open source Enterprise Service Bus ESBMT6

Seismicity Based Reservoir Characterization Case Study ... · of the hydraulic diffusivity characterizing a geothermal or hydrocarbon reservoir on the large spatial scale (on the

CHARACTERIZING BEHAVIOUR