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This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Grant Agreement n°633999
Copyright © 2014, EUCLID Consortium
Horizon 2020 Programme
SFS-03b-2014 EU-China cooperation on IPM in agriculture
EU-CHINA Lever for IPM Demonstration
Project ID: 633999
Deliverable number : D4.1
Deliverable title : Field demonstration yearly report - 2016
EC version : V1
Due date of milestone M24
Actual submission date 31/05/2018 (M33)
Ref. Ares(2018)2845287 - 31/05/2018
DOCUMENT INFO
1. Author(s)
Organisation name lead contractor ACTA
Author Organisation e-mail Philippe DELVAL ACTA philippe.delval@acta.asso.fr
2. Revision history
Version Date Modified by Comments 1 04/01/2018 Philippe Delval Starting version 2 26/03/2018 Philippe Delval Corrected version
3. Dissemination level
PU Public
CO Confidential, only for members of the consortium (excluding the Commission Services)
EUCLID - Europe-China Lever for IPM Demonstration info@euclidipm.org.com - www.euclidipm.org
EXECUTIVE SUMMARY
Background Every year, a field demonstration report describing the evolution of the trials and the demonstration/field days organized by the partners in Europe and China will be published on the website and disseminated to a large audience
Objectives The overall objective of the field demonstration report is to describe the activity of the previous year and to show what is plan the next one.
Methods Every year, a report will summarize the trials implemented and the work done into the demonstration trial network
Summary
This report describe the trial implemented in Italy in 2017 and the work done to prepare the demonstration trials of 2018:
- Organization of the three crop groups - Phone meetings - Information put in the workspace: methodology, innovation sheets,
phone meeting reports
EUCLID - Europe-China Lever for IPM Demonstration info@euclidipm.org.com - www.euclidipm.org
Table of contents
Introduction ............................................................................................................................................................................. 5
1. Field demonstrations trials in 2017 ..................................................................................................................... 5
1.1. Introduction ......................................................................................................................................................5
1.2. Description of the trial ......................................................................................................................................5
2. Crop groups ................................................................................................................................................................. 13
2.1. Introduction ................................................................................................................................................... 13
2.2. Lettuce crop group members ........................................................................................................................ 13
2.3. Tomato crop group members ........................................................................................................................ 14
2.4. Grapevine crop group members.................................................................................................................... 15
3. Work done in 2017 ................................................................................................................................................... 16
1. Introduction ................................................................................................................................................... 16
2. Phone meetings ............................................................................................................................................. 16
3. Information on the workspace ...................................................................................................................... 16
4. Euclid innovation sheet ................................................................................................................................. 17
4. Work planned in 2018 ............................................................................................................................................ 17
1. Demonstration trials ...................................................................................................................................... 17
3. Key points ...................................................................................................................................................... 17
EUCLID - Europe-China Lever for IPM Demonstration info@euclidipm.org.com - www.euclidipm.org
Introduction
The first two years of the EUCLID project was mostly allocated to research and development work. As no exploitable results for field trials have been formulated since September 2017 (last annual EUCLID meeting), the main work of WP4 was to prepare the network of demonstration sites and the grid for field trial descriptions (responsible, location, technology tested, crop, etc.). Nevertheless, one greenhouse trial has been run by ANT in Italy in 2017 in order to assess the efficiency of inorganic salts, compost, biostimulants and new BCA developed by the company on lettuce crop. The first part of this deliverable is allocated to the description of this trial. The second part of the deliverable presents: (i) the organization of crop groups which are groups of work joining to organize field trials around one specific crop and (ii) the work done into this groups so far.
1. Field demonstrations trials in 2017
1.1. Introduction
In 2016, just one demonstration sites organizes trial. It was in Italy on lettuce.
1.2. Description of the trial
The efficacy of a number of BCA’s, inorganic salt as resistance inducers, biofertilizer and compost
have been used in order to foster their practical application in a preventative way against lettuce
Fusarium wilt, using two lettuce cultivars moderately susceptible to F. oxysporum f.sp. lactucae Race
1 in two type of soil, in two demonstration sites in Piedmont (Italy).
Products used
Different compounds that are, phosphite-based fertilizer, compost, experimental and commercial
biocontrol agents (BCAs) were compared to azoxystrobin used as chemical control.
Inorganic salt
Potassium phosphite;
Organic amendments based compost
Ant’s Compost V, AgriNewTech, Torino, Italy
Ant’s Compost M AgriNewTech, Torino, Italy
Biofertilizer:
K-Humixol AgriNewTech, Torino, Italy:
EUCLID - Europe-China Lever for IPM Demonstration info@euclidipm.org.com - www.euclidipm.org
Help Plus. AgriNewTech, Torino, Italy
Biocontrol agents (BCA’s)
Bacillus subtilis
Trichoderma asperellum +T. gamsii
Mixture of three bacterial isolates of Pseudomonas putida from Agroinnova’s collection;
Trichoderma sp. from AgriNewTech (Italy).
Type and timing of treatments
The tested products based on inorganic salt, BCA’s, biofertilizer were applied on lettuces at the
nursery level by spraying the soil of each plug tray. Four applications at 5-day intervals were carried
out (Figure 2).
The products based on compost (Ant’s Compost V and Ant’s Compost M) were applied at the plug
tray at T0 and then were mixed into the soil immediately before transplanting (Figures 1 and 3).
The organic fertilizer K-Humixol was applied at T0 in nursery, at T7 and at T14 in nursery and/or by
root dipping at lettuce transplanting. Azoxystrobin was applied only once.
Plant material
Two lettuce varieties belonging to different types have been selected because widely grown in
commercial farms for their marketable value.
Seeds of lettuce were sown in 100-plug trays using a peat mix substrate in a commercial nursery
(Figure 1).
Figure 1 –Treatments in a commercial nursery in Piedmont.
EUCLID - Europe-China Lever for IPM Demonstration info@euclidipm.org.com - www.euclidipm.org
Figure 2. Development of lettuce in treated tray at the nursery level.
Figure 3. Compost application before lettuce transplant and transplant of lettuce.
EUCLID - Europe-China Lever for IPM Demonstration info@euclidipm.org.com - www.euclidipm.org
Site of the trials
Trial 1 was carried out under plastic tunnel (360 m2) at Moretta (CN) in a farm with a history in
lettuce cultivation with several lettuce cycles prior to the beginning of this study, in a soil naturally
infested with the race 1 of Fusarium oxysporumf.sp. lactucae (Figure 4).
Trial 2 was carried out under plastic tunnel (90 m2) at Carmagnola (TO) in the experimental farm of
the University of Torino, in a soil artificially infested with the pathogen, using a highly virulent strain
of F. oxysporum f. sp. lactucae, belonging to race 1, incorporated into the soil by rototilling seven days
before transplanting (Figure 5).
The treated lettuce plants, 21 days old, were transplanted at a density of 12 and 16 plants/m2 per
both cultuvar, representing the experimental unit. Plot were arranged in a complete randomized
block design in each trial, with four replicated trials.
Lettuce plants were irrigated and grown according to the cultural practices adopted by commercial
growers in the region.
EUCLID - Europe-China Lever for IPM Demonstration info@euclidipm.org.com - www.euclidipm.org
Figure 4- Demostration trial in soil naturally infested by F.oxysporum f.sp. lactucae.
1 –Compost application;
2- Soil mulching and plot design;
3- Symptoms evaluation 10-days after
transplanting;
4-Lettuce development 20-days after
transplanting;
5-End of the trial and samples.
1 2
3 4
5
EUCLID - Europe-China Lever for IPM Demonstration info@euclidipm.org.com - www.euclidipm.org
2
Figure 5- Demonstration trial in soil artificially infested by F.oxysporum f.sp. lactucae.
1- Lettuce grown in the demonstration
tunnel 15-days after transplanting;
2-Lettuce development 25-days after
transplanting;
3-Lettuce development 40-days after
transplanting.
4-End of the trial and samples;
5-Fusarium wilt severity score.
1
2 3
4 5
5
EUCLID - Europe-China Lever for IPM Demonstration info@euclidipm.org.com - www.euclidipm.org
Results and general comment
Lettuce plants were monitored weekly for disease development. The number of infected plants
showing wilting and stem necrosis was counted to assess disease incidence. The totally wilted (dead)
plants were removed (Figure 6) The final disease rating was made six weeks after transplanting by
dissecting each plant. At the end of the trials, the total biomass was weighed in order to evaluate the
effect of the tested treatments on the yield.
Figure 6- Symptoms of Fusarium wilt on lettuce.
Generally, in both the demonstration trials the tested products provided significant results in
disease control. Lettuce yield under field conditions varied with the treatments, with the lowest
yield in the untreated plots and fresh weight of lettuce resulted significantly higher using nursery
treatments in comparison to that of the untreated control plots (Figure 7).
EUCLID - Europe-China Lever for IPM Demonstration info@euclidipm.org.com - www.euclidipm.org
Figure 7. Effect of nursery treatments on Fusarium wilt on lettuce Batavian type. Data are expressed as fresh weight (g/10 plants per plot) at the end trial 1.
In order to approove the design developed to test the technologies, during the trials technicians and
the nurseryman working in the leafy vegetables sector got to see the trials in progress. In October
19th, the demonstration sites have been visited by Corinne Pons (Adviser on Vegetables in the
Chamber of Agriculture, Alpes-Maritimes) and by Justine GARNODIER Ingénieur experimentation
(CTIFL / APREL).
0
500
1000
1500
2000
2500
3000
3500
Fresh weight
EUCLID - Europe-China Lever for IPM Demonstration info@euclidipm.org.com - www.euclidipm.org
2. Crop groups
2.1. Introduction
During the discussions around WP4 organization in Almeria, 2017 annual meeting of the EUCLID
project, the descision was taken to work in “crop groups” in order to discuss the technologies to
test and the available fields where to run the trials. People from WP1, 2 (research and
development), WP3 (field trials demonstration) and WP4 (technologies evaluation) are working in
these different groups.
These crops groups were constituted after the 2017 annual meeting and were followed directly be
meetings between November 2017 and January 2018 in order to plan the field works for 2018.
Three crop groups were established: lettuce, tomato and grape. In order to get high quality
experimental methods, each of these groups are composed by different members of the EUCLID
project (mostly researchers and agronomists) but also technicians, crop specialists and companies
coming from different countries targeted by the EUCLID project.
2.2. Lettuce crop group members
Country Name & e-mail address Partner Role France Jean-François BOURGEAY
jean-francois.bourgeay@inra.fr Marc BARDIN marc.bardin@inra.fr Philippe NICOT philippe.nicot@inra.fr
INRA Avignon Research & test
Benjamin GARD gard@ctifl.fr Justine GARNODIER Garnodier@ctifl.fr
CTIFL Applied research
Pauline BODIN Pauline.bodin@acta.asso.fr
ACTA Management
Italy Giovanna GILARDI giovanna.gilardi@unito.it
AGROINNOVA Research & test
Massimo PUGLIESE ceo@agrinewtech.com
AGRINEWTECH Research & test Wp3
Spain Judit ARNO judit.arno@irta.cat Cristina CASTANE cristina.castane@irta.cat
IRTA Applied research
Sweden Thomas RICARD thomas.ricard@binab.se
BINAB Industry & test
UK Adam WALKER adam.walker@oxitec.com
OXITEC Industry
Adrian LEACH IMPERIAL COLLEGE Wp3
EUCLID - Europe-China Lever for IPM Demonstration info@euclidipm.org.com - www.euclidipm.org
a.w.leach@imperial.ac.uk Paul BARANOWSKI paul.baranowski11@imperial.ac.uk
China Yang NIAN-WAN yangnianwan@caas.cn
CAAS Research
Damien DESCHRYVER damien_deschryver@carrefour.com
CARREFOUR China Test
2.3. Tomato crop group members
Country Name & e-mail address Partner Role France Jean-François BOURGEAY
jean-francois.bourgeay@inra.fr Marc BARDIN marc.bardin@inra.fr Philippe NICOT philippe.nicot@inra.fr
INRA Avignon Research & test
Eva THOMINE eva.thomine@inra.fr
INRA Sophia Research & test
Benjamin GARD gard@ctifl.fr
CTIFL Applied research
Lionel CHAZELLE l.chazelle@rougeline.com
Rougeline Test
Pauline BODIN Pauline.bodin@acta.asso.fr
ACTA Management
Italy Giovanna GILARDI giovanna.gilardi@unito.it
AGROINNOVA Research & test
Massimo PUGLIESE ceo@agrinewtech.com
AGRINEWTECH Research & test Wp3
Spain Judit ARNO judit.arno@irta.cat Cristina CASTANE cristina.castane@irta.cat
IRTA Applied research
Ramon ALBAJES ralbajes@pvcf.udl.cat Filipe MADEIRA fmadeira@pvcf.udl.cat
UdL Research
Sweden Thomas RICARD thomas.ricard@binab.se
BINAB Industry & test
UK Adrian LEACH a.w.leach@imperial.ac.uk Paul BARANOWSKI paul.baranowski11@imperial.ac.uk
IMPERIAL COLLEGE Wp3
China Yin Quan-LIU yqliu@zju.edu.cn
ZJU Research
Yang NIAN-WAN yangnianwan@caas.cn
CAAS Research
Damien DESCHRYVER damien_deschryver@carrefour.com
CARREFOUR China Test
EUCLID - Europe-China Lever for IPM Demonstration info@euclidipm.org.com - www.euclidipm.org
2.4. Grapevine crop group members
Country Name & e-mail address Partner Role France David LAFOND
david.lafond@vignevin.com Sébastien CODIS sebastien.codis@vignevin.com Eric SERRANO Eric.serrano@vignevin.com Esteban FORTIN esteban.fortin@vignevin.com
IFV Applied research
Adrien RUSCH adrien.rusch@inra.fr
INRA Bordeaux Research
Pauline BODIN Pauline.bodin@acta.asso.fr
ACTA Management
Italy Massimo PUGLIESE ceo@agrinewtech.com
AGRINEWTECH Research & test Wp3
Spain Santiago PLANAS santiago.planas@udl.cat Carla ROMAN carlaroman@eagrof.udl.cat
UdL Research & test
UK Adrian LEACH a.w.leach@imperial.ac.uk Paul BARANOWSKI paul.baranowski11@imperial.ac.uk
IMPERIAL COLLEGE Wp3
China Yang NIAN-WAN yangnianwan@caas.cn
CAAS Research
Damien DESCHRYVER damien_deschryver@carrefour.com
CARREFOUR China Test
EUCLID - Europe-China Lever for IPM Demonstration info@euclidipm.org.com - www.euclidipm.org
3. Work done in 2017
1. Introduction
The work consisted in gathering information on the workspace and organizing phone meetings to plan the field trials in 2018.
2. Phone meetings
Two phone meetings were organized in late 2017: - November 2017, 21st : lettuce group - December 2017, 11 : tomato group - January 2018, 29th : grapevine group
3. Information on the workspace
Access to the demonstration sites informations are available on the following link:
https://intranet.inra-transfert.fr/euclid/_layouts/15/start.aspx#/SitePages/WP4.aspx
In each crop demo sites session, two types of information are available: (i) general information
where templates and general information from Pure projects can be consulted (see figure …) and
(ii) crop group information were demonstration sites and crop groups meetings are described.
Figure 8. Capture of the workspace screen – WP4 General documents
In the crop group section, information concerning each group are available:
- Complete description of the demonstration sites
- Euclid innovation sheets
- Phone meeting reports
Additional information is planned to be added in these sections. These information will mostly be
on protocols, demonstration trials reports and other useful information (sampling for residues
analyses methodology, etc.).
EUCLID - Europe-China Lever for IPM Demonstration info@euclidipm.org.com - www.euclidipm.org
4. Euclid innovation sheet
During the annual meeting, the consortium decided that each research partner must provide a description of their innovation and instructions to be implemented into the demonstrations trials. The template was produced in October and sent to all partners to fill it with their information. The information depicted give information on: 1/ Type of innovation: product or technology 2/ Status (if the product/technology is: registered, authorized for experimentation, not authorized) 3/ Targets (pests, diseases) and rates of product application for each crop 4/ Recommendations of use: storage conditions, operator precautions, mixing instructions, application instructions, restriction of use, deadline to use it 5/ Partner contact The following table shows the number of innovations available for 2018 demonstration trials for each target crop:
Lettuce Tomato Grapevine Products 6 8 3 Technology 0 0 1
4. Work planned in 2018
1. Demonstration trials
The following table shows the partners per countries responsible of the demonstration trials that might be implemented in 2018:
Lettuce Tomato Grapevine France INRA
APREL INRA IFV
Italy ANT+AGRO ANT+AGRO ANT Spain IRTA IRTA UdL Sweden BINAB BINAB ?
3. Key points
During the phone meetings, several key points to implement the trials were defined:
1) The objective of the demonstration trials is to compare 2 programs: IPM Euclid package vs Conventional package. To design it, the person responsible of the experiment must refer to the IPM Packages excel files produced in WP3;
2) The choice of the programs and Euclid innovation depends on pests and diseases for each trial; 3) Untreated areas are not mandatory because the main objective is to compare Euclid solutions
with conventional ones;
EUCLID - Europe-China Lever for IPM Demonstration info@euclidipm.org.com - www.euclidipm.org
4) Artificial infestation are not mandatory: the objective is to compare the IPM packages in the natural and the whole cropping period conditions;
5) Observations of pests and diseases will be conducted describing the intensity ; 6) Evaluation of commercial standards characteristics will be done during the harvest.
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