SFS-03b-2014 EU-China cooperation on IPM in agriculture · Marc BARDIN [email protected] Philippe NICOT [email protected] INRA Avignon Research & test Eva THOMINE [email protected]

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This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Grant Agreement n°633999

Copyright © 2014, EUCLID Consortium

Horizon 2020 Programme

SFS-03b-2014 EU-China cooperation on IPM in agriculture

EU-CHINA Lever for IPM Demonstration

Project ID: 633999

Deliverable number : D4.1

Deliverable title : Field demonstration yearly report - 2016

EC version : V1

Due date of milestone M24

Actual submission date 31/05/2018 (M33)

Ref. Ares(2018)2845287 - 31/05/2018

DOCUMENT INFO

1. Author(s)

Organisation name lead contractor ACTA

Author Organisation e-mail Philippe DELVAL ACTA [email protected]

2. Revision history

Version Date Modified by Comments 1 04/01/2018 Philippe Delval Starting version 2 26/03/2018 Philippe Delval Corrected version

3. Dissemination level

PU Public

CO Confidential, only for members of the consortium (excluding the Commission Services)

mailto:[email protected]

EUCLID - Europe-China Lever for IPM Demonstration [email protected] - www.euclidipm.org

EXECUTIVE SUMMARY

Background Every year, a field demonstration report describing the evolution of the trials and the demonstration/field days organized by the partners in Europe and China will be published on the website and disseminated to a large audience

Objectives The overall objective of the field demonstration report is to describe the activity of the previous year and to show what is plan the next one.

Methods Every year, a report will summarize the trials implemented and the work done into the demonstration trial network

Summary

This report describe the trial implemented in Italy in 2017 and the work done to prepare the demonstration trials of 2018:

- Organization of the three crop groups - Phone meetings - Information put in the workspace: methodology, innovation sheets,

phone meeting reports


Table of contents

Introduction ............................................................................................................................................................................. 5

1. Field demonstrations trials in 2017 ..................................................................................................................... 5

1.1. Introduction ......................................................................................................................................................5

1.2. Description of the trial ......................................................................................................................................5

2. Crop groups ................................................................................................................................................................. 13

2.1. Introduction ................................................................................................................................................... 13

2.2. Lettuce crop group members ........................................................................................................................ 13

2.3. Tomato crop group members ........................................................................................................................ 14

2.4. Grapevine crop group members.................................................................................................................... 15

3. Work done in 2017 ................................................................................................................................................... 16

1. Introduction ................................................................................................................................................... 16

2. Phone meetings ............................................................................................................................................. 16

3. Information on the workspace ...................................................................................................................... 16

4. Euclid innovation sheet ................................................................................................................................. 17

4. Work planned in 2018 ............................................................................................................................................ 17

1. Demonstration trials ...................................................................................................................................... 17

3. Key points ...................................................................................................................................................... 17


Introduction

The first two years of the EUCLID project was mostly allocated to research and development work. As no exploitable results for field trials have been formulated since September 2017 (last annual EUCLID meeting), the main work of WP4 was to prepare the network of demonstration sites and the grid for field trial descriptions (responsible, location, technology tested, crop, etc.). Nevertheless, one greenhouse trial has been run by ANT in Italy in 2017 in order to assess the efficiency of inorganic salts, compost, biostimulants and new BCA developed by the company on lettuce crop. The first part of this deliverable is allocated to the description of this trial. The second part of the deliverable presents: (i) the organization of crop groups which are groups of work joining to organize field trials around one specific crop and (ii) the work done into this groups so far.

1. Field demonstrations trials in 2017

1.1. Introduction

In 2016, just one demonstration sites organizes trial. It was in Italy on lettuce.

1.2. Description of the trial

The efficacy of a number of BCA’s, inorganic salt as resistance inducers, biofertilizer and compost

have been used in order to foster their practical application in a preventative way against lettuce

Fusarium wilt, using two lettuce cultivars moderately susceptible to F. oxysporum f.sp. lactucae Race

1 in two type of soil, in two demonstration sites in Piedmont (Italy).

Products used

Different compounds that are, phosphite-based fertilizer, compost, experimental and commercial

biocontrol agents (BCAs) were compared to azoxystrobin used as chemical control.

Inorganic salt

Potassium phosphite;

Organic amendments based compost

Ant’s Compost V, AgriNewTech, Torino, Italy

Ant’s Compost M AgriNewTech, Torino, Italy

Biofertilizer:

K-Humixol AgriNewTech, Torino, Italy:


Help Plus. AgriNewTech, Torino, Italy

Biocontrol agents (BCA’s)

Bacillus subtilis

Trichoderma asperellum +T. gamsii

Mixture of three bacterial isolates of Pseudomonas putida from Agroinnova’s collection;

Trichoderma sp. from AgriNewTech (Italy).

Type and timing of treatments

The tested products based on inorganic salt, BCA’s, biofertilizer were applied on lettuces at the

nursery level by spraying the soil of each plug tray. Four applications at 5-day intervals were carried

out (Figure 2).

The products based on compost (Ant’s Compost V and Ant’s Compost M) were applied at the plug

tray at T0 and then were mixed into the soil immediately before transplanting (Figures 1 and 3).

The organic fertilizer K-Humixol was applied at T0 in nursery, at T7 and at T14 in nursery and/or by

root dipping at lettuce transplanting. Azoxystrobin was applied only once.

Plant material

Two lettuce varieties belonging to different types have been selected because widely grown in

commercial farms for their marketable value.

Seeds of lettuce were sown in 100-plug trays using a peat mix substrate in a commercial nursery

(Figure 1).

Figure 1 –Treatments in a commercial nursery in Piedmont.


Figure 2. Development of lettuce in treated tray at the nursery level.

Figure 3. Compost application before lettuce transplant and transplant of lettuce.


Site of the trials

Trial 1 was carried out under plastic tunnel (360 m2) at Moretta (CN) in a farm with a history in

lettuce cultivation with several lettuce cycles prior to the beginning of this study, in a soil naturally

infested with the race 1 of Fusarium oxysporumf.sp. lactucae (Figure 4).

Trial 2 was carried out under plastic tunnel (90 m2) at Carmagnola (TO) in the experimental farm of

the University of Torino, in a soil artificially infested with the pathogen, using a highly virulent strain

of F. oxysporum f. sp. lactucae, belonging to race 1, incorporated into the soil by rototilling seven days

before transplanting (Figure 5).

The treated lettuce plants, 21 days old, were transplanted at a density of 12 and 16 plants/m2 per

both cultuvar, representing the experimental unit. Plot were arranged in a complete randomized

block design in each trial, with four replicated trials.

Lettuce plants were irrigated and grown according to the cultural practices adopted by commercial

growers in the region.


Figure 4- Demostration trial in soil naturally infested by F.oxysporum f.sp. lactucae.

1 –Compost application;

2- Soil mulching and plot design;

3- Symptoms evaluation 10-days after

transplanting;

4-Lettuce development 20-days after

transplanting;

5-End of the trial and samples.

1 2

3 4

5


2

Figure 5- Demonstration trial in soil artificially infested by F.oxysporum f.sp. lactucae.

1- Lettuce grown in the demonstration

tunnel 15-days after transplanting;


transplanting;


transplanting.

4-End of the trial and samples;

5-Fusarium wilt severity score.

1

2 3

4 5

5


Results and general comment

Lettuce plants were monitored weekly for disease development. The number of infected plants

showing wilting and stem necrosis was counted to assess disease incidence. The totally wilted (dead)

plants were removed (Figure 6) The final disease rating was made six weeks after transplanting by

dissecting each plant. At the end of the trials, the total biomass was weighed in order to evaluate the

effect of the tested treatments on the yield.

Figure 6- Symptoms of Fusarium wilt on lettuce.

Generally, in both the demonstration trials the tested products provided significant results in

disease control. Lettuce yield under field conditions varied with the treatments, with the lowest

yield in the untreated plots and fresh weight of lettuce resulted significantly higher using nursery

treatments in comparison to that of the untreated control plots (Figure 7).


Figure 7. Effect of nursery treatments on Fusarium wilt on lettuce Batavian type. Data are expressed as fresh weight (g/10 plants per plot) at the end trial 1.

In order to approove the design developed to test the technologies, during the trials technicians and

the nurseryman working in the leafy vegetables sector got to see the trials in progress. In October

19th, the demonstration sites have been visited by Corinne Pons (Adviser on Vegetables in the

Chamber of Agriculture, Alpes-Maritimes) and by Justine GARNODIER Ingénieur experimentation

(CTIFL / APREL).

0

500

1000

1500

2000

2500

3000

3500

Fresh weight


2. Crop groups

2.1. Introduction

During the discussions around WP4 organization in Almeria, 2017 annual meeting of the EUCLID

project, the descision was taken to work in “crop groups” in order to discuss the technologies to

test and the available fields where to run the trials. People from WP1, 2 (research and

development), WP3 (field trials demonstration) and WP4 (technologies evaluation) are working in

these different groups.

These crops groups were constituted after the 2017 annual meeting and were followed directly be

meetings between November 2017 and January 2018 in order to plan the field works for 2018.

Three crop groups were established: lettuce, tomato and grape. In order to get high quality

experimental methods, each of these groups are composed by different members of the EUCLID

project (mostly researchers and agronomists) but also technicians, crop specialists and companies

coming from different countries targeted by the EUCLID project.

2.2. Lettuce crop group members

Country Name & e-mail address Partner Role France Jean-François BOURGEAY

[email protected] Marc BARDIN [email protected] Philippe NICOT [email protected]

INRA Avignon Research & test

Benjamin GARD [email protected] Justine GARNODIER [email protected]

CTIFL Applied research

Pauline BODIN [email protected]

ACTA Management

Italy Giovanna GILARDI [email protected]

AGROINNOVA Research & test

Massimo PUGLIESE [email protected]

AGRINEWTECH Research & test Wp3

Spain Judit ARNO [email protected] Cristina CASTANE [email protected]

IRTA Applied research

Sweden Thomas RICARD [email protected]

BINAB Industry & test

UK Adam WALKER [email protected]

OXITEC Industry

Adrian LEACH IMPERIAL COLLEGE Wp3














[email protected] Paul BARANOWSKI [email protected]

China Yang NIAN-WAN [email protected]

CAAS Research

Damien DESCHRYVER [email protected]

CARREFOUR China Test

2.3. Tomato crop group members

Country Name & e-mail address Partner Role France Jean-François BOURGEAY

[email protected] Marc BARDIN [email protected] Philippe NICOT [email protected]

INRA Avignon Research & test

Eva THOMINE [email protected]

INRA Sophia Research & test

Benjamin GARD [email protected]

CTIFL Applied research

Lionel CHAZELLE [email protected]

Rougeline Test


ACTA Management

Italy Giovanna GILARDI [email protected]

AGROINNOVA Research & test

Massimo PUGLIESE [email protected]


Spain Judit ARNO [email protected] Cristina CASTANE [email protected]

IRTA Applied research

Ramon ALBAJES [email protected] Filipe MADEIRA [email protected]

UdL Research

Sweden Thomas RICARD [email protected]

BINAB Industry & test

UK Adrian LEACH [email protected] Paul BARANOWSKI [email protected]

IMPERIAL COLLEGE Wp3

China Yin Quan-LIU [email protected]

ZJU Research

Yang NIAN-WAN [email protected]

CAAS Research



























2.4. Grapevine crop group members

Country Name & e-mail address Partner Role France David LAFOND

[email protected] Sébastien CODIS [email protected] Eric SERRANO [email protected] Esteban FORTIN [email protected]

IFV Applied research

Adrien RUSCH [email protected]

INRA Bordeaux Research


ACTA Management

Italy Massimo PUGLIESE [email protected]


Spain Santiago PLANAS [email protected] Carla ROMAN [email protected]

UdL Research & test

UK Adrian LEACH [email protected] Paul BARANOWSKI [email protected]

IMPERIAL COLLEGE Wp3

China Yang NIAN-WAN [email protected]

CAAS Research

















3. Work done in 2017

1. Introduction

The work consisted in gathering information on the workspace and organizing phone meetings to plan the field trials in 2018.

2. Phone meetings

Two phone meetings were organized in late 2017: - November 2017, 21st : lettuce group - December 2017, 11 : tomato group - January 2018, 29th : grapevine group

3. Information on the workspace

Access to the demonstration sites informations are available on the following link:

https://intranet.inra-transfert.fr/euclid/_layouts/15/start.aspx#/SitePages/WP4.aspx

In each crop demo sites session, two types of information are available: (i) general information

where templates and general information from Pure projects can be consulted (see figure …) and

(ii) crop group information were demonstration sites and crop groups meetings are described.

Figure 8. Capture of the workspace screen – WP4 General documents

In the crop group section, information concerning each group are available:

- Complete description of the demonstration sites

- Euclid innovation sheets

- Phone meeting reports

Additional information is planned to be added in these sections. These information will mostly be

on protocols, demonstration trials reports and other useful information (sampling for residues

analyses methodology, etc.).

https://intranet.inra-transfert.fr/euclid/_layouts/15/start.aspx#/SitePages/WP4.aspx


4. Euclid innovation sheet

During the annual meeting, the consortium decided that each research partner must provide a description of their innovation and instructions to be implemented into the demonstrations trials. The template was produced in October and sent to all partners to fill it with their information. The information depicted give information on: 1/ Type of innovation: product or technology 2/ Status (if the product/technology is: registered, authorized for experimentation, not authorized) 3/ Targets (pests, diseases) and rates of product application for each crop 4/ Recommendations of use: storage conditions, operator precautions, mixing instructions, application instructions, restriction of use, deadline to use it 5/ Partner contact The following table shows the number of innovations available for 2018 demonstration trials for each target crop:

Lettuce Tomato Grapevine Products 6 8 3 Technology 0 0 1

4. Work planned in 2018

1. Demonstration trials

The following table shows the partners per countries responsible of the demonstration trials that might be implemented in 2018:

Lettuce Tomato Grapevine France INRA

APREL INRA IFV

Italy ANT+AGRO ANT+AGRO ANT Spain IRTA IRTA UdL Sweden BINAB BINAB ?

3. Key points

During the phone meetings, several key points to implement the trials were defined:

1) The objective of the demonstration trials is to compare 2 programs: IPM Euclid package vs Conventional package. To design it, the person responsible of the experiment must refer to the IPM Packages excel files produced in WP3;

2) The choice of the programs and Euclid innovation depends on pests and diseases for each trial; 3) Untreated areas are not mandatory because the main objective is to compare Euclid solutions

with conventional ones;


4) Artificial infestation are not mandatory: the objective is to compare the IPM packages in the natural and the whole cropping period conditions;

5) Observations of pests and diseases will be conducted describing the intensity ; 6) Evaluation of commercial standards characteristics will be done during the harvest.

Documents

SFS-03b-2014 EU-China cooperation on IPM in agriculture · Marc BARDIN [email protected] Philippe NICOT [email protected] INRA Avignon Research & test Eva THOMINE [email protected]