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Recombinant maxi-K Channels on Transistor, A Prototype of Iono -electronic Interfacing. Authors: B. Straub, E. Meyer, and P. Fromherz Presenter: Todd Finkler. Disclaimer. - PowerPoint PPT Presentation
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Recombinant maxi-K Recombinant maxi-K ChannelsChannels
on Transistor, A Prototype of on Transistor, A Prototype of Iono-electronic InterfacingIono-electronic Interfacing
Authors: B. Straub, E. Meyer, and P. Fromherz
Presenter: Todd Finkler
DisclaimerDisclaimerYou are about to enter the world of the unknown. The presenter’s background is in computer engineering, more specifically in networks. NOT in biological engineering or biology or medical or in any field that would make this topic easy. However, this topic is interesting to the presenter, so the presenter is wading forth into an area of unknown. If you chose to enter, you do so at your own risk. You may or may not get correct answers to your questions. You may or may not walk away from this talk more confused than when you entered the room. There are some really smart people who are examining this field and the presenter is not one of them. If you cannot read this, tough. If you can read this, I did not make this small enough. My bad. The presenter will not be held responsible for any misguided teachings from this presentation. If you cannot agree to this, you know where the door is since you came through the door to get here. This disclaimer is meant for entertainment purposes only, and is by no means intended to be serious. If, up to this point, you are still reading this and you still think this is serious, you need to take a break from school – especially since it is still so early in the semester. And if you are still reading this, you have a much longer attention span than I have. But that is good, because you will probably one of the few who actually pay attention to the whole presentation. Thanks for paying attention this long. Thanks also to those who have supported my efforts to this point, including Weiwei for asking a barrage of questions while I was developing these slides. Additionally, thanks to Koranan for her patience in getting this presentation at the last minute. And finally, thanks also to Professor Newcomb for hopefully giving me an “A” in this class for the semester. Enough with this nonsense and lets move on with the presentation. The following is a repeat of the above. You are about to enter the world of the unknown. The presenter’s background is in computer engineering, more specifically in networks. NOT in biological engineering or biology or medical or in any field that would make this topic easy. However, this topic is interesting to the presenter, so the presenter is wading forth into an area of unknown. If you chose to enter, you do so at your own risk. You may or may not get correct answers to your questions. You may or may not walk away from this talk more confused than when you entered the room. There are some really smart people who are examining this field and the presenter is not one of them. If you cannot read this, tough. If you can read this, I did not make this small enough. My bad. The presenter will not be held responsible for any misguided teachings from this presentation. If you cannot agree to this, you know where the door is since you came through the door to get here. This disclaimer is meant for entertainment purposes only, and is by no means intended to be serious. If, up to this point, you are still reading this and you still think this is serious, you need to take a break from school – especially since it is still so early in the semester. And if you are still reading this, you have a much longer attention span than I have. But that is good, because you will probably one of the few who actually pay attention to the whole presentation. Thanks for paying attention this long. Thanks also to those who have supported my efforts to this point, including Weiwei for asking a barrage of questions while I was developing these slides. Additionally, thanks to Koranan for her patience in getting this presentation at the last minute. And finally, thanks also to Professor Newcomb for hopefully giving me an “A” in this class for the semester. Enough with this nonsense and lets move on with the presentation. The following is a repeat of the above. You are about to enter the world of the unknown. The presenter’s background is in computer engineering, more specifically in networks. NOT in biological engineering or biology or medical or in any field that would make this topic easy. However, this topic is interesting to the presenter, so the presenter is wading forth into an area of unknown. If you chose to enter, you do so at your own risk. You may or may not get correct answers to your questions. You may or may not walk away from this talk more confused than when you entered the room. There are some really smart people who are examining this field and the presenter is not one of them. If you cannot read this, tough. If you can read this, I did not make this small enough. My bad. The presenter will not be held responsible for any misguided teachings from this presentation. If you cannot agree to this, you know where the door is since you came through the door to get here. This disclaimer is meant for entertainment purposes only, and is by no means intended to be serious. If, up to this point, you are still reading this and you still think this is serious, you need to take a break from school – especially since it is still so early in the semester. And if you are still reading this, you have a much longer attention span than I have. But that is good, because you will probably one of the few who actually pay attention to the whole presentation. Thanks for paying attention this long. Thanks also to those who have supported my efforts to this point, including Weiwei for asking a barrage of questions while I was developing these slides. Additionally, thanks to Koranan for her patience in getting this presentation at the last minute. And finally, thanks also to Professor Newcomb for hopefully giving me an “A” in this class for the semester. Enough with this nonsense and lets move on with the presentation. The following is a repeat of the above.
Joining Computer and Joining Computer and BrainBrainWhy Important?The challenge – different charge
carriers◦Computer – electrons in solid ion
lattice◦Brain – electrons in polar fluid
What Cell to Use?HEK293 – What?
◦Human Embryonic Kidney cell line with sheared adenovirus 5 DNA
HEK293 – Why?◦Conductance properties◦Well-defined ion channel
What Interface?
human slowpoke (hSlo) maxi-KCa channel◦ Perfectly controlled by
patch-clamp◦ Displays high single-
channel conductance yielding large membrane current.
◦ Is target for estradiol – potentially useful for biosensor
Applying Voltage to the Applying Voltage to the CellCell
58 mV
53 mV
45 mV
40 mV
30 mV
-23 mV
-68 mV
Relations
VJ = RJAJMgJM(VM – V0) where: VJ is the extracellular voltage in the gapRJ is the gap’s ohmic resistanceAJM is the area of the attached membranegJM is the average specific K+ conductance
IM = AMgM(VM – V0)
VJ / IM is constant experiementally, so…
gJM / gM must be constant for all VM
And…And…Boltzmann’s equation can describe their voltage-dependent gating
g/gbar = 1/(1 + e[z(V0.5 – VM)/Vth] ) where…
z is the gating charge
V0.5 is the half-maximum activation at a max specific gbar
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