Quantitative analysis of cytoskeletal network structures fileCytoskeleton Keratin filaments. Role of...

Quantitative analysis of

cytoskeletal network structures

5 µm

MTMF

IF (keratin)

microfilaments(MF)

persistence tensile length [µm] modulus [a.u.]

MF 18 300MT >100 150IF 1 1

intermediatefilaments (IF)

microtubules(MT)

Cytoskeleton

Keratin filaments

Role of cytoskeletal filaments

1. cell and tissue architecture

2. cell migration

Motility of leukaemia cells - vimentin

ATRAPMA

NB4 - Vimentin

neutrophil

Retinoic Acid Syndrome

Tensegrity (R.B. Fuller, D. Ingber)

microtubulesmicrotubules

intermediate filaments

F-actin

substrate anchor

Keratin networks in microgravity

t2µg

control sphingosylphosphorylcholine (SPC)

Regulation of keratin networks

min

F-actin

K8/18

Keratin network morphology

perinuclear

periphery

50 ng/mL TGFα

min

ERK

2 ac

tivat

ion

Seufferlein et al. 1999

control

TGFα (30 min)

Keratin network reorganisation in response to TGFα

control

TGFα, 30 min

control TGFα control TGFα

Structural features of keratin networks

Fitting of random tessellation models

Poisson-Line (PLT) Poisson-Voronoi (PVT) Poisson-Delaunay (PDT)

Iterated tesselations

Bernoulli thinning

Model selected as best fitcontrol TGFα

control PVT / PLT

TGFα PDT / PLT

Actin networks in lamellipodia

Elasticity ~ mesh size4.4

PVT PDT PLT

F-actin

Migration

Stephanie Eckel, Frank Fleischer, Hendrik Schmidt, Volker SchmidtDept. of Stochastics, University Ulm

Paul WaltherDept. of Electron Microscopy, University Ulm

Thomas SeufferleinDept. of Internal Medicine I, University Ulm

Bishr OmaryDept. of Medicine, Stanford University

Tatjana SvitkinaDept. of Biology, University of Pennsylvania

Joachim SpatzDept. of Physical Chemistry, University Heidelberg