Mukunda

Research and Development of Research and Development of Agricultural Biotechnology in Agricultural Biotechnology in

NepalNepal

Dr. Mukunda Ranjit Academician(RONAST)

and General Secretary

(NBA)

IntroductionPlant Tissue CultureBio-fertilizerBio PesticidesDisease diagnosticsMolecular CharacterizationHRD in BiotechnologyConclusion

Agriculture Mainstay R&D Ag Biotech Traditional Biotech

– Yeast and Brewery Production– Vegetable fermentation– Dairy Production– Mushroom – Agaricus, Pleurotus, Shitake

Modern Biotech– Plant Tissue Culture– Bio Fertilizer– Disease Diagnostics & Vaccine Production

Public Institutions

– DPR– NARC– RONAST– FDD– CVL– IAVS, CDB/TU– KU/PU

Private

– Botanical Enterprises

– Nepal Biotech Nursery

– RLABB– Microplants– GREAT Nepal– HIMBORCE

Plant Tissue CulturePlant Tissue Culture

Protocols for 100 plant speciesMicro-propagation of virus-free

Potato, Banana, Strawberry, Citrus rootstocks, Large Cardamoms, Mums, Orchids etc

Potato for elimination of PVA, PVM, PVS, PVX, PVY, PLRV & PSTVd

Banana for elimination of BBTV & CMV

Citrus Rootstocks from Meristem culture

Citrus

Micro-grafting

For elimination of CTV & CGD

Candidatus Liberibacter asiaticus + CTV

Adult and instars of Diaphorina citri

Seedling Yellows of Eureka lemon

Caused by CTV

Citrus Brown Aphid adult & nymphs

Toxoptera citricida

Vector of CTV

Cardamom

Disease DiagnosticsDisease Diagnostics

By Biological Indexing and PCR

Citrus – CGD:Madame Vinous Sweet Orange and Ponkan

Mandarin

By DAS ELISA

•Potato – PVA, PVM, PVS, PVX, PVY and PLRV•Citrus – CTV: Mexican Lime•L. Cardamom – Chhirke and Furke: PRSV antibodies

Disease EliminationDisease Elimination Potato viruses eliminated by TT and AMC by

PRP, NARC and GREAT PVX and PVY eliminated from Petronese and

Multa by GREAT CTV and CGD eliminated from Mandarin by

micrografting, GREAT Chhirke and Furke eliminated from RS, GS and

DS by GREAT

Bio fertilizers and Bio Bio fertilizers and Bio PesticidesPesticides

RIT for Legumes, RONAST and NARC MIT for composting, RONAST Biogas production from Eupatorium and Water

Hyacinth by RECAST Endo and Ecto Mychorrizae forest soil conservation Three BT strains to control lepidepteron pests on

crucifer (Pieris brassicae nepalensis ) Biopesticides from Eupatorium, Lantana and

Azadirachta PPR vaccine by tissue culture

Molecular CharacterizationMolecular Characterization

Nepal has 0.1% land but 2% flowering plants

700 species of medicinal and aromatic plants

200 species of endemicMC of Swertia and CamelliaMC of Bacillus thurengensesMC of Fygopyrum & Oryza sativa

HRD in BiotechHRD in Biotech

CDB, TU – Post graduate training on TC and plant Biotech

KU/PU – 4 years B.Tech. On BiotechIAVS – M.Sc. Thesis on Agriculture

Biotech – Needs Advanced level training (Ph.D., Post

doc) on plant, animal and microbial biotech, immunology and molecular biology

Conclusions:Conclusions:1. Zero virus PBS production for doubling

the yield of potato2. Production of CTV & CGD-free grafted

plants of mandarin to solve the citrus decline problem

3. Aggressive use of bio-fertilizers4. Production of antibodies and vaccines5. MC of economically imp. crops

THANK YOUTHANK YOU