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Dr. Mukunda Ranjit Academician (RONAST) and General Secretary (NBA) Research and Development ofResearchandDevelopmentof Agricultural Biotechnology inAgriculturalBiotechnologyin NepalNepal Introduction Plant Tissue Culture Bio-fertilizer Bio Pesticides Disease diagnostics Molecular Characterization HRD in Biotechnology Conclusion
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Research and Development of Research and Development of Agricultural Biotechnology in Agricultural Biotechnology in
NepalNepal
Dr. Mukunda Ranjit Academician(RONAST)
and General Secretary
(NBA)
IntroductionPlant Tissue CultureBio-fertilizerBio PesticidesDisease diagnosticsMolecular CharacterizationHRD in BiotechnologyConclusion
Agriculture Mainstay R&D Ag Biotech Traditional Biotech
– Yeast and Brewery Production– Vegetable fermentation– Dairy Production– Mushroom – Agaricus, Pleurotus, Shitake
Modern Biotech– Plant Tissue Culture– Bio Fertilizer– Disease Diagnostics & Vaccine Production
Public Institutions
– DPR– NARC– RONAST– FDD– CVL– IAVS, CDB/TU– KU/PU
Private
– Botanical Enterprises
– Nepal Biotech Nursery
– RLABB– Microplants– GREAT Nepal– HIMBORCE
Plant Tissue CulturePlant Tissue Culture
Protocols for 100 plant speciesMicro-propagation of virus-free
Potato, Banana, Strawberry, Citrus rootstocks, Large Cardamoms, Mums, Orchids etc
Potato for elimination of PVA, PVM, PVS, PVX, PVY, PLRV & PSTVd
Banana for elimination of BBTV & CMV
Citrus Rootstocks from Meristem culture
Citrus
Micro-grafting
For elimination of CTV & CGD
Candidatus Liberibacter asiaticus + CTV
Adult and instars of Diaphorina citri
Seedling Yellows of Eureka lemon
Caused by CTV
Citrus Brown Aphid adult & nymphs
Toxoptera citricida
Vector of CTV
Cardamom
Disease DiagnosticsDisease Diagnostics
By Biological Indexing and PCR
Citrus – CGD:Madame Vinous Sweet Orange and Ponkan
Mandarin
By DAS ELISA
•Potato – PVA, PVM, PVS, PVX, PVY and PLRV•Citrus – CTV: Mexican Lime•L. Cardamom – Chhirke and Furke: PRSV antibodies
Disease EliminationDisease Elimination Potato viruses eliminated by TT and AMC by
PRP, NARC and GREAT PVX and PVY eliminated from Petronese and
Multa by GREAT CTV and CGD eliminated from Mandarin by
micrografting, GREAT Chhirke and Furke eliminated from RS, GS and
DS by GREAT
Bio fertilizers and Bio Bio fertilizers and Bio PesticidesPesticides
RIT for Legumes, RONAST and NARC MIT for composting, RONAST Biogas production from Eupatorium and Water
Hyacinth by RECAST Endo and Ecto Mychorrizae forest soil conservation Three BT strains to control lepidepteron pests on
crucifer (Pieris brassicae nepalensis ) Biopesticides from Eupatorium, Lantana and
Azadirachta PPR vaccine by tissue culture
Molecular CharacterizationMolecular Characterization
Nepal has 0.1% land but 2% flowering plants
700 species of medicinal and aromatic plants
200 species of endemicMC of Swertia and CamelliaMC of Bacillus thurengensesMC of Fygopyrum & Oryza sativa
HRD in BiotechHRD in Biotech
CDB, TU – Post graduate training on TC and plant Biotech
KU/PU – 4 years B.Tech. On BiotechIAVS – M.Sc. Thesis on Agriculture
Biotech – Needs Advanced level training (Ph.D., Post
doc) on plant, animal and microbial biotech, immunology and molecular biology
Conclusions:Conclusions:1. Zero virus PBS production for doubling
the yield of potato2. Production of CTV & CGD-free grafted
plants of mandarin to solve the citrus decline problem
3. Aggressive use of bio-fertilizers4. Production of antibodies and vaccines5. MC of economically imp. crops
THANK YOUTHANK YOU