MRSA Diagnostics - Lawrence Technological University ... · PDF fileMRSA Diagnostics Kaitlyn...

MRSA DiagnosticsKaitlyn Tingley & Amanda BukhtiaLawrence Technological University

Advisors: Dr. Li, Dr. Nasir, Dr. Bou-Akl and Dr. Auner

What is MRSA?● Methicillin-resistant

Staphylococcus aureus(MRSA)

● An antibiotic resistant staph infection

● Enter the bloodstream through open wounds leading to death

http://www.cdc.gov/mrsa/tracking/index.html

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Need Statement

“Develop a quick and cost effective method to detect if a patient has

(MRSA)”

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Market Value

Stakeholders•Hospitals•Nursing Homes•Schools•Patients

Market Value•In VA hospitals alone the market value is 30.94 million

http://www.mayoclinic.org/diseases-conditions/mrsa/basics/definition/con-20024479http://www.aaos.org/news/aaosnow/mar09/clinical13.asp

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Current Diagnostic Methods4

http://laboratory-manager.advanceweb.com/Archives/Article-Archives/MRSA-Screening-Culture-or-Molecular.aspxhttp://ac.els-cdn.com/S0956566311005197/1-s2.0-S0956566311005197-main.pdf?_tid=55edafa6-5648-11e4-beaa-00000aacb35f&acdnat=1413583120_a5737397e845f21a215313800649bf76http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1287783/ http://www.rapidmicrobiology.com/test-method/methicillin-resistant-staphylococcus-aureus-mrsa-detection-and-identification-methods/http://www.biomedcentral.com/1471-2180/6/83https://workspace.imperial.ac.uk/opticalandsemidev/public/Publications/LAWES.pdfhttp://www.aist.go.jp/aist_e/latest_research/2012/20121121/20121121.html

Diagnostic Steps

1. Separation2. Concentration3. Detection

Lowy, Franklin. "STAPHYLOCOCCUS AUREUS INFECTIONS." New England Journal of Medicine (1998): 520-32. Print. http://www.medicinenet.com/staph_infection/page5.htm#what_is_antibiotic-resistant_staph_aureus

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Biological InteractionsPenicillin-Binding Protein 2a

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http://jb.asm.org/content/187/5/1815.fullhttp://www.britannica.com/EBchecked/topic/27783/antibody

MRSA

AntiPBP2a

MRSANonMRSA

Anti PBP1AntiPBP2a

Design Concept

.

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Slide 8

1 Finish NotesKaitlyn Tingley,

Project Tasks

1. Device Fabrication 2. Antibody Immobilization3. Simulation to determine flow parameters 4. Device Testing

82

Slide 9

2 Finish NotesKaitlyn Tingley,

Electrode Fabrication

• Substrate Material: Glass (slide)

93

Slide 10

3 Finish NotesKaitlyn Tingley,

Flow Channel Fabrication

● Soft Lithography

104

Slide 11

4 Finish NotesKaitlyn Tingley,

Antibody Immobilization and Device Bonding11

1. Antibody Immobili-zation 2. Device Bonding

Device Testing12

Adapted from Dr. Nasir

Inlet Outlet

PDMS

Glass

Au Electrodes Non-MRSAMRSA

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Slide 13

5 Finish NotesKaitlyn Tingley,

Testing Parameters

• Staph SolutionMSSA (Control)MRSA(Variable)

• Input: Staph Solution

• Output: Impedance

1367

Slide 14

6 Finish NotesKaitlyn Tingley,

7 Double check last sentenceKaitlyn Tingley,

Validation Testing

http://www.inphotonics.com/raman.htmHarper, Chem Commun,2012, 48(75):9412-4

Raman Spectroscopy

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Blue is MRSARed is Non-MRSA

Expected Deliverables

● Develop a prototype ● Test functionality ● Submit provisional intellectual patent ● Submit abstract to national BMES

conferenceThis image cannot currently be displayed.

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Future Design Application

1 2 3

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http://www.nature.com/srep/2013/130521/srep01863/full/srep01863.html#supplementary-informationhttp://www.sciencedirect.com/science/article/pii/S1549963413005893

Timeline17

Budget

http://www.biocompare.com/pfu/110447/soids/19788/Antibodies/PBP2ahttp://www.hometrainingtools.com/checkout/cart/http://www.everbrightsolar.net/sylgard-184-silicone-elastomer-kit-encapsulation-184.htmlhttp://www.walmart.com/ip/Ranger-Pipette-Pop-Refill-Pack-25-pcs/26682149http://www.amazon.com/Jensen-Global-Syringe-Manual-Calibrated/dp/B002R18F02/ref=sr_1_8?ie=UTF8&qid=1418330917&sr=8-8&keywords=30+gauge+syringehttp://www.amazon.com/TOOGOO-Plastic-Stainless-Dispenser-0-176mm/dp/B00J2N3OK4/ref=sr_1_5?ie=UTF8&qid=1418331020&sr=8-5&keywords=30+gauge+needles#product-description-iframehttp://www.amazon.com/Latex-Tubing-I-D-O-D-WALL/dp/B0045V36PQhttp://www.abcam.com/pbp-antibody-ab58224.html

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Team Structure

Amanda Kaitlyn

OSHA Training

Fabricating

Fluid Dynamics

Antibody Immobilization

Testing

Device Design

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Acknowledgements I.)Wayne State University’s Smart Sensors and Integrated Microsystems (SSIM)

a.Dr. Auner II.)Lawrence Technological University

a.Dr. Li b.Dr. Nasir c. Dr. Bou- Akl

III.)The LEGENDS Entrepreneurial Student Awards

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Questions?