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ISEET‐2016 Conference Program
Start End Friday July 1 2016
17:00 ‐ 18:15
Registration / Drinks / Commercial Booths Visit / Posters Viewing / Visit of the Old Library of the Sint Augustijnen Abby
18:15 ‐ 19:00
Welcome address and Keynote Lecture The Horse Catalyzed Birth of Modern Veterinary Medicine in 18th Century France – Peter Bols
19:00 ‐ 22:00 Gala Dinner / Visit of the Old Library Saturday July 2 2016
Session 1 ‐ Jean‐François Bruyas (Moderator)
8:30 ‐ 9:00 Cryopreservation of Equine Embryos – Ed Squires
9:00 ‐ 9:15
Easy, quick and cheap technique to cryopreserve Welsh B pony blastocyst – F. Guignot – Abstract #6
9:15 ‐ 9:30
Vitrification of large equine embryos following manual or micromanipulator‐assisted blastocoele collapse – R. Ferris – Abstract #31
9:30 ‐ 9:45
In vitro evaluation by DAPI staining of fresh, cooled and vitrified donkey embryos ‐ Duccio Panzani – Abstract #45
9:45 ‐ 10:00
Toxicity of a vitrification protocol for equine immature oocytes – H. DeSiqueira Canesin– Abstract #63
10:00 ‐ 10:45 Coffee
Session 2 ‐ Tom Stout (Moderator)
10:45 ‐ 11:00
Preliminary morphokinetic annotation data for early equine embryo development in vitro using a time‐lapse imaging system – K. Schnauffer – Abstract #47
11:00 ‐ 11:15
Combining Time‐Lapse Imaging and Next Generation RNA‐Sequencing to Assess Equine Embryo Developmental Potential – S. Chavez – Abstract #65
11:15 ‐ 11:45
Clinical Applications of Preimplantation Genetic Testing in Equine, Bovine and Human Embryos – Carolina Herrera
11:45 ‐ 12:00
Application of ultrasound technique in early fetal gender determination – the field study – S. Martinez Zuviria – Abstract #15
12:00 ‐ 12:15
Influences of conceptus sex on its development during early equine pregnancy – C. Aurich – Abstract #10
12:15 ‐ 13:45 Lunch
Session 3 ‐ Claudia Klein (Moderator)
13:45 ‐ 14:00
Results and limitations on the use of sex‐sorted semen in conventional ET programs in Polo Argentino breed – A. Lascombes – Abstract #67
14:00
‐
14:15
Overnight Shipping of equine oocytes from remote locations to an ART laboratory enables access to the flexibility of ovum pick up ICSI and embryo cryopreservation technologies ‐ C. Galli – Abstract #68
14:15 ‐ 14:30
Effects of different media systems and osmolarity on equine blastocyst development after ICSI ‐ Young Ho Choi – Abstract #22
14:30 ‐ 14:45
Successful in vitro production of mammalian embryos: a strict quality management approach – C. Herrera – Abstract #52
14:45 ‐ 15:00
Production of equine embryos in vitro using conventional intracytoplasmic sperm injection and a complete human embryo culture system ‐ C.A. Leisinger – Abstract #60
15:00 ‐ 15:45 Coffee
Session 4 ‐ Katrin Hinrichs (Moderator)
15:45 ‐ 16:15 In vitro Production of Equine Embryos and Cloning: Today’s Status – Marc Maserati and Adrian Mutto
16:15 ‐ 16:30 Rescuing equine cloned embryos by aggregation – A. Gambini – Abstract #27
16:30 ‐ 16:45
Enrichment of somatic cells from frozen‐thawed equine semen using fluorescence‐activated cell sorting – J.G. Brom‐de‐Luna – Abstract #56
16:45 ‐ 17:00
Variability in efficiency in a commercial oocyte transfer program – F. Riera – Abstract #9
17:00 ‐ 17:15
Pregnancy rate and pregnancy loss after transfer of in vivo or in vitro derived equine embryos – P. McCue – Abstract #42
17:15 ‐ 19:45 Dinner on your own in the city ‐ 5‐10 min walk to Graslei with many restaurants
19:45 ‐ 22:00 Workshop ‐ two parallel workshops on ET and IVP
Workshop 1 ‐ Clinical aspects of embryo transfer ‐ McCue, Riera, Aurich
Workshop 2 ‐ In vitro production of equine embryos ‐ Hererra, Maserati
Sunday July 3 2016
Session 5 ‐ Luis Losinno (Moderator)
8:30 ‐ 9:00 Maternal Recognition of Pregnancy in the Context of Equine Embryo Transfer – Claudia Klein
9:00 ‐ 9:15 Effects of suppression of estrogen synthesis during early pregnancy in mares – B. A. Ball – Abstract #11
9:15 ‐ 9:30 Embryo quality grade and recipient ovulation rate affect pregnancy rates in embryo transfer recipient mares – H.G. Pedersen – Abstract #24
9:30 ‐ 9:45 Effects of asynchronous embryo transfer on the endometrial transcriptome in mares – C. Gibson – Abstract #43
9:45 ‐ 10:00 Association between equine sperm parameters and ICSI outcome – R. Gonzalez‐Castro – Abstract #25
10:00 ‐ 10:45 Coffee Session 6 ‐ Young Ho Choi (Moderator)
10:45 ‐ 11:00 Glucose uptake and lactate production of equine cumulus‐oocyte complexes (COC’s) during in vitro maturation (IVM) – J. Len – Abstract #19
11:00 ‐ 11:15 Effect of pyruvate addition and oxygen concentration on carbohydrate metabolism during in vitro maturation of equine oocytes – N. Lewis – Abstract #30
11:15 ‐ 11:30 Effects of verbascoside treatment during oocyte in vitro maturation on blastocyst development and bioenergetic/oxidative status after ICSI in the horse – N.A. Martino – Abstract #34
11:30 ‐ 11:45 Developmental Competence of Equine Oocytes is Associated with Alterations in their Cumulus Proteome – J. Walter – Abstract #59
11:45 ‐ 12:30 Genome Editing in Large Animals – James West
12:30 ‐ 13:45 Lunch Session 7 ‐ Cezare Galli (Moderator)
13:45 ‐ 14:00 Maturation Condition alters the Cumulus Proteome of Equine Oocytes – F. Huwiler – Abstract #62
14:00 ‐ 14:15 The effect of a simulated physiological oocyte maturation system on the in vitro production of equine embryos – E.S. Metcalf – Abstract #32
14:15 ‐ 14:30 Dynamics of in vitro maturation of equine oocytes recovered by transvaginal follicular aspiration – M.B. Rodríguez – Abstract #8
14:30 ‐ 14:45 In Vitro Production of Equine Embryos by Ovum Pick Up and Intracytoplasmic Sperm Injection in Young Mares – R. Sanchez – Abstract #58
14:45 ‐ 15:00 Factors influencing oocyte recovery and in‐vitro production of equine embryos in a commercial OPU/ICSI program – A. Claes – Abstract #39
15:00 ‐ 15:30 Coffee Session 8 ‐ Peter Daels (Moderator)
15:30 ‐ 16:15 Gametes, Embryos and their Epigenome: Considerations for Equine Embryo
Technologies – Pascal Chavatte
16:15 ‐ 16:30 Effect of the interval from Prostaglandin F2α treatment to ovulation on Embryo Recovery Rate and subsequent Pregnancy Rate in a commercial Equine Embryo Transfer program ‐ J. Cuervo Arango – Abstract #17
16:30 ‐ 16:45 Factors Affecting Embryo Recovery: Retrospective Study based on Commercial Embryo Transfer – E. Squires – Abstract #23
16:45 ‐ 17:00 Doppler ultrasound for assessment of follicular response to induction of ovulation in mares – M. Alvarenga – Abstract #33
17:00 ‐ 17:15 Dominant follicle ablation as a way of preparing embryo recipient mares – F. Saules Ignácio – Abstract #50
17:15 ‐ 17:30 Closing remarks and planning for the future
17:30 ‐ 22:00 Buffet Dinner
22:00 ‐ End of the ISEET‐2016 Conference
ISEET‐2016 Conference Posters Abstract # ‐ Title – Authors Posters will be available for viewing throughout the conference
1 ‐ Intrauterine administration of coconut oil to early pregnant mares increases embryonic vesicle
size ‐ Sandra Wilsher and WR Allen
2 ‐ Effect of Mesenchymal Stem Cells on Equine Ovarian Follicular Development ‐ Sicilia T Grady,
Ashlee E Watts, and Katrin Hinrichs
3 ‐ Is spontaneously double ovulation associate with incidence of EPL after nonsurgical embryo
transfer in mares? ‐ Hamed Farnia, N. Largel, S.M. Zuviria, J.I. Lopez Echenique
4 ‐ The Effect of Eight Antioxidants on Cryopreserved Equine Sperm Quality ‐ Zhuangyuan Wu, Jingbo
Chen, Yongming Luo, Xinbao Zheng, Hong Dong, Fei Huo
5 ‐ Comparison between open and closed vitrification system in equine collapsed embryos ‐ Weiss, J.
Blanco, M. Sanchez, R. Arbouin, C. Schockemöhle, P.
7 ‐ Expression of mesenchymal stem/stromal cell markers and steroid hormone receptors in the
equine endometrium ‐ Rink, E., Kuhl J., Aurich C., French H., Nino‐Fong R., Watson E., Donadeu F.X.
12 ‐ Cooling equine embryo for 24h prior to vitrification: What are the consequences on its viability?
‐ Maud Caillaud, Estelle Provost And Florence Guignot
13 ‐ Efficiency of two prostaglandin F2α analogs for synchronization of estrus and ovulation in mares
‐ Juliane Kuhl, Jörg E. Aurich, Christine Aurich
14 ‐ Comparison of the efficacy of different single doses of buserelin with hCG for timed ovulation
induction in the mare. ‐ Newcombe, JR and Cuervo‐Arango, J
16 ‐ Embryo Transfer and its influence on the Polo Argentino breed in the last 25 years Martinez ‐
Zuviria S. and Zagrajczuk A.
18 ‐ The influences of age, laminitis and serum ACTH concentration on embryo recovery from
Arabian mares ‐ Jenny Katrine Boye, Valentina Barone and JG Oriol
20 ‐ Thawed stallion sperm refrozen without cryoprotectants activates cow oocytes after
intracytoplasmic sperm injection (ICSI) ‐ Jose Len, Dave Kleeman, Jen Kelly
21 ‐ Application of flunixin meglumine at the time of embryo transfer and the effect on the fertility ‐
Okada, C. T. C., Teixeira Martini Segabinazi, L. G., Alvarenga, M. A.
26 ‐ Assessment of fetal sex determination in early equine pregnancy by isolation of fetal cells from
the maternal circulation ‐ Martin Köhne, Michaela Prchal‐Murphy, Martin Hofer, Ralf Steinborn,
Christine Aurich
28 ‐ Attempts to improve synchronization of ovulation and induce superovulation in the mare ‐
Shuaishuai Wu, Nan Li, Shumin Wang, Guocai Han, and Shenming Zeng
29 ‐ Ultrasonographic evaluation of fetal growth between full siblings Arabian foals gestating in their
genetic dams and embryo transfer recipients. Merridale‐Punter M, Galinelli NC and Oriol JG
35 ‐ Proteomic analysis of the cervical mucus plug during pregnancy in mares ‐ Loux S.C., Scoggin K. ,
Troedsson M.H. , Squires E.L. , Ball B.A.
36 ‐ Report of frozen equine embryos transfer in the Czech Republic ‐ Josef Muller, Jindrich Muller
37 ‐ Influence of mare parity on weight, height, thoracic circumference and vitality of neonatal foals ‐
FJ Affonso, MG Meirelles, MA Alonso, CF Guimaraes, KM Lemes, M Nichi, CB Fernandes
38 ‐ Effect of maternal age on estrogen production in mid to late pregnancy in the mare ‐ MA Alonso,
FJ Affonso, MG Meirelles, R Simas, DF Souza, VV Hernandes, M Nichi, MN Eberlin, CB Fernandes
40 ‐ Potential to reduce negative effects of exercise stress on number of pregnancies in a commercial
embryo transfer program. ‐ Pinto M., Miragaya M., Burns P., Douglas R. and Neild D.
41 ‐ Can pregnancy survival after embryo transfer be predicted based on early ultrasound
examinations in the recipient mare? ‐ Morrissey JK, Ferris RA, Trundell D, Loncar K, Burden C, Scofield
D, McCue PM
44 ‐ Influence of insemination on regulatory T cells in the peripheral blood of estrous mares ‐
Constanze Hartmann, Wilhelm Gerner, Armin Saalmüller, Christine Aurich
46 ‐ Comparative efficacy of hCG or GnRH analogue (lecirelin acetate) on follicular dynamics, degree
of endometrial edema, sexual behavior, ovulation and pregnancy rate in crossbred broodmares ‐
Fayzan Sultan Awan, Muhammad Usman Mehmood, Abdul Sattar, Nasim Ahmad
48 ‐ Can we predict fertility in prepubertal female horses using Anti‐Müllerian hormone? ‐ Dragos
Scarlet, Natascha Ille, Manuela Wulf, Ingrid Walter, Christine Aurich
49 ‐ The effect of exercise of the donor mare in an embryo transfer program ‐ M. Christoffersen, M.
Dige Soegaard, H. Gervi Pedersen and R. Munk Andersen
51 ‐ Effect of firocoxib on ovulation and fertility rates of embryo donor mares ‐ Friso AM, Cyrino MA,
Teoro MT, Alvarenga MA
53 ‐ In vitro growth of small equine embryos after vitrification ‐ Celina M Checura and Alexis Powers
54 ‐ Preimplantation Gender Determination on Equine Embryos using LAMP ‐ P. Dini, M. Van Poucke,
C. Herrera, L. Peelman and P. Daels
55 ‐ Comparison of pregnancy rates between acyclic mules and cyclic mares as recipients for embryo
transfer ‐ C.E. Camargo, R.C. Macan, S.F. Rechsteiner, E.L. Gastal
57 ‐ Gene expression prior and after vitrification of equine CCO’s ‐ Polenz M.F., Pereira G.R., Augusto
C.G., Fiala Rechsteiner S.
61 ‐ Holding Equine Oocytes in Syngro® Embryo Holding Medium at 4°C. ‐ P. Dini, O. Bogado, M.
Hostens and P. Daels
64 ‐ Improving frozen equine sperm motility by the addition of different concentrations of Coenzyme
Q10 to a freezing extender ‐ Lagares MA, Luz SB, Coutinho IM, Morais BM and Stahlberg R
66 ‐ Transport of Equine Oocytes in Syngro® Embryo Holding Medium using a Passive Cooling System
(Equitainer®) ‐ Kaatje Danielle Ducheyne, Francisco Guaimas, Romina Santa Cruz, Julieta Mertian,
Nicolás Matiás Ortega, Peter Daels and Adrián Mutto
69 ‐ Efficacy of a single injection of buserelin acetate in saline solution and a single injection of hCG
for the induction of ovulation in mares ‐ L. Normandin, C. Frilley, A. Cochard, H Terris, J.‐F. Bruyas
70 ‐ Is there a repeatability of the size of follicles at the ovulation within individuals in mares ‐ C.
Lefrançois, J.‐F. Bruyas
72 ‐ Oocytes from aged mares show reduced expression of mRNA for key spindle assembly
checkpoint components ‐ M. Rizzo, M. de Ruijter‐Villani, C. Deelen, M. Beitsma, S. Cristarella, M.
Quartuccio, T.A.E. Stout
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