IDENTIFYING THE MELANOMA WAVELENGTHS E. De Fabo, EOH, SPHHS, GWUMCdrmecd@gwumc.edu 202-994-3975

IDENTIFYING THE MELANOMA IDENTIFYING THE MELANOMA WAVELENGTHSWAVELENGTHS

Wavelength280 320 360 400

IRR

AD

IAN

CE

(W

/cm

2 /n

m)

1e-7

1e-6

1e-5

1e-4

1e-3

San Diego, CAJuly 4, 2000

UVAUVB

(nm)

WAVELENGTH (nm)

250 260 270 280 290 300 310 320 330

IRR

AD

IAN

CE

(W

/cm

2/n

m)

1e-7

1e-6

1e-5

1e-4

Wavelength (nm)

300 400 500 600 700 800 900

Irra

dia

nc

e (

W/c

m2/n

m)

1e-9

1e-8

1e-7

1e-6

1e-5

1e-4

Solar Simulator

Wavelength (nm)

250 300 350 400 450

Irra

dia

nc

e (

W/c

m2

/nm

)

1e-7

1e-6

1e-5

1e-4

UVA UVB

E. De Fabo, EOH, SPHHS, GWUMCE. De Fabo, EOH, SPHHS, GWUMC drmecd@gwumc.edu drmecd@gwumc.edu 202-994-3975202-994-3975

BLACK ALBINO

20/24(3.7)

6/18(1.0)

E. De Fabo, EOH, SPHHS, GWUMCE. De Fabo, EOH, SPHHS, GWUMC

Effect of pigment on melanomaEffect of pigment on melanoma

SPD PHILLIPS F40 SUNLAMPS (6)MEASURED BY OPTRONICS 742 SPECTRORADIOMETER

Wavelength (nm)

200 300 400 500 600 700 800 900

Irra

dia

nce

(W

/cm

2 /nm

)

1e-11

1e-10

1e-9

1e-8

1e-7

1e-6

1e-5

1e-4

1e-3

UVB UVA

C57BL6 HGF/SF

FVB HGF/SF

C57BL6 HGF/SF

STRAIN 1

STRAIN 2

FVB HGF/SF

E. De Fabo, EOH, SPHHS, GWUMCE. De Fabo, EOH, SPHHS, GWUMC De Fabo et al., Cancer Res., 2004; in prep.

GFP Induction 24 Hrs Post-DoxGFP Induction 24 Hrs Post-Dox R.Zaidi, NCI,NIHR.Zaidi, NCI,NIH

• Pups were injected doxycycline on the day of birth.Pups were injected doxycycline on the day of birth.

• Back skins were harvested 24 hours after the doxycycline injection Back skins were harvested 24 hours after the doxycycline injection

and imaged for GFP.and imaged for GFP.

Dct-rtTA– / GFP+ Dct-rtTA+ / GFP+

E. De Fabo, EOH, SPHHS, GWUMCE. De Fabo, EOH, SPHHS, GWUMC

UVB + ALBINO

STRAIN-1

UVA + PIGMENT

STRAIN-2

UVB + PIGMENT

STRAIN-2

UVA + ALBINO

STRAIN-1

E. De Fabo, EOH, SPHHS, GWUMCE. De Fabo, EOH, SPHHS, GWUMC

ACKNOWLEDGEMENTSACKNOWLEDGEMENTS• THE GEORGE WASHINGTON UNIVERSITY THE GEORGE WASHINGTON UNIVERSITY

SCHOOL OF PUBLIC HEALTH & HEALTH SERVICESSCHOOL OF PUBLIC HEALTH & HEALTH SERVICES

FRANCES NOONANFRANCES NOONAN

JESSE BAHN JESSE BAHN

SCHOOL OF MEDICINE & HEALTH SCIENCESSCHOOL OF MEDICINE & HEALTH SCIENCES

TERESA HAWLEYTERESA HAWLEY

ALI RAMEZANIALI RAMEZANI

• NIH/NCINIH/NCI

RAZA ZAIDI: Laboratory of Cancer Biology & RAZA ZAIDI: Laboratory of Cancer Biology & GeneticsGenetics

GLENN MERLINO: Chief, Laboratory of Cancer GLENN MERLINO: Chief, Laboratory of Cancer Biology & GeneticsBiology & Genetics

PAUL MELTZER: Chief, Cancer Genetics BranchPAUL MELTZER: Chief, Cancer Genetics Branch

• Funding- Funding- NCI/NIH; Elaine Snyder Cancer Research NCI/NIH; Elaine Snyder Cancer Research Fund, GWUMCFund, GWUMC

E. De Fabo, EOH, SPHHS, GWUMCE. De Fabo, EOH, SPHHS, GWUMC