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Histology
• Light Microscopy (LM) – paraffin embedded, 7 micron sections, specific stains
• Transmission Electron Microscopy (TEM) – plastic embedded, 70 millimicron sections, e-dense stains
• Scanning Electron Microscopy (SEM) – critically point dried, gold coated
Light Microscopy
TEM
SEM
Epithelium
Simple Epithelium
Simple Squamous
Mesothelium
Simple Squamous (Mesothelium) by TEM
Simple Squamous – Endothelium (En)
Simple Squamous – Endothelium (Ed) by TEM
Capillary (endothelial cell) by TEM
Simple Cuboidal Epithelium (longitudinal section)
Simple Cuboidal (cross section)
Simple Cuboidal by TEM
Simple Columnar with Goblet Cells
Simple Columnar (cross section)
with Goblet Cells (arrow)
Goblet Cell with PAS stain (red)
Simple Columnar with Goblet Cells
(Mu) by TEM
Simple Columnar (AC) with Goblet Cells
(GC) by SEM
Simple Columnar mucus
secreting
Simple Columnar mucus
secreting
Epithelium
Stratified Epithelium
Pseudostratified Ciliated Epithelium with Goblet Cells
Pseudostratified Epithelium with Cilia (a) and Goblet Cells (b)
Stratified Squamous non-Keratinized Epithelium
Transitional Epithelium
Transitional Epithelium with rounded binucleated surface cells
Stratified Squamous Keratinized (thin)
Stratified Squamous Keratinized (thick)
Epithelial Cell Junctions
Tight Junctions (Zonula Occludens) by TEM
Tight Junctions (Zonula Occludens) by Freeze Fracture
Gap Junction (Nexus) by TEM
Gap Junction
Connexin moleculesOpen pores for
ions, molecules, etc
2 nM gap between membranes
TEM of Zonula Adherens (red
arrows)
TEM of a Desmosome (blue
arrow)
TEM (colorized) of Adherens Junctions
TEM of a Desmosome with tonofibrils
TEM of a Hemi-Desmosome
Summary of the maintenance of cell shape by junctions and cytoskeletal elements
Glandular Epithelium
Serous Gland with ducts (arrows)
Serous secretory unit (high magnification)
Mucous Gland with duct (arrow)
Mixed Gland with Serous Demilunes (SD)
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