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BcuI. IR. tnp. IR. pGP353. lacZ. ori colE1. 7652 bps. aacA-aphD. Bam. HI. Sma. I. Xma. I. Eco. RI. Promoter Analyses in Mycoplasma pneumoniae. Cloning of potential promoter fragments Integration of the lacZ -fusion into the chromosome by transposition - PowerPoint PPT Presentation
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Cloning of potential promoterfragments
Integration of the lacZ-fusioninto the chromosome bytransposition
Assay of LacZ activities on plates and in liquid media
Promoter Analyses in Mycoplasma pneumoniae
pGP3537652 bps
BamHISmaIXmaIEcoRI
IR
lacZ
aacA-aphD
ori colE1
IR
tnp
BcuI
ldh
MPN673
-10-35
-35-10
+81-160
+81-118
+81-84
+81-53
HindIII HindIII
-160 +81+1-85
0 200 400 600 800 1000
LacZ activity [nmol o- NP/min*mg]
Analysis of the ldh Promoter
The lacZ fusion reflects promoter activity
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