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Cloning of potential promote fragments Integration of the lacZ-fusio into the chromosome by transposition Assay of LacZ activities on plates and in liquid media Promoter Analyses in Mycoplasma pneumoniae pGP353 7652 bps Bam HI Sma I Xma I EcoRI IR lacZ aacA-aphD ori colE1 IR tnp BcuI

Cloning of potential promoter fragments Integration of the lacZ -fusion into the chromosome by

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BcuI. IR. tnp. IR. pGP353. lacZ. ori colE1. 7652 bps. aacA-aphD. Bam. HI. Sma. I. Xma. I. Eco. RI. Promoter Analyses in Mycoplasma pneumoniae. Cloning of potential promoter fragments Integration of the lacZ -fusion into the chromosome by transposition - PowerPoint PPT Presentation

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Page 1: Cloning of potential promoter fragments Integration of the lacZ -fusion into the chromosome by

Cloning of potential promoterfragments

Integration of the lacZ-fusioninto the chromosome bytransposition

Assay of LacZ activities on plates and in liquid media

Promoter Analyses in Mycoplasma pneumoniae

pGP3537652 bps

BamHISmaIXmaIEcoRI

IR

lacZ

aacA-aphD

ori colE1

IR

tnp

BcuI

Page 2: Cloning of potential promoter fragments Integration of the lacZ -fusion into the chromosome by

ldh

MPN673

-10-35

-35-10

+81-160

+81-118

+81-84

+81-53

HindIII HindIII

-160 +81+1-85

0 200 400 600 800 1000

LacZ activity [nmol o- NP/min*mg]

Analysis of the ldh Promoter

The lacZ fusion reflects promoter activity

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Professione Promoter

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