Chapter 14. Examples of Homonuclear 2D Experiments

Amino Acids

Proteins

Ubiquitin, a small protein ( 8.5kd ) present in all eukaryotic cells . It contains a highly conserved sequence of 76 amino acids that is identical in a wide variety of sources including humans, fish, and insects. It participates in diverse cellular functions, such as protein degradation, chromatin structure, and heat shock, by conjugation to other proteins through the carboxy terminus. This protein is highly conserved in evolution : yeast and human ubiquitin differ at only 3 of 76 residues. The carboxyl-terminal glycine of ubiquitin becomes covalently attached to the ε-amino group of lysine residues of proteins destined to be degraded.

The energy for the formation of these isopeptide bonds comes from ATP. Three enzymes- E1 : ubiquitin activating enzyme、 E2 : ubiquitin-carrier protein、 E3 : ubiquitin-protein ligase- participate in the conjugation of ubiquitin to protein. First, the terminal carboxylate group of ubiquitin becomes linked to a sulfhydryl group of E1 by a thioester bond. Activated ubiquitin is then shuttled to a sulfhydryl of E2. Finally, E3 catalyzes the transfer of ubiquitin from E2 to the target protein. A protein tagged for destriction usually acquires several molecules of ubiquitin. Theε-amino group of a lysine residue of one ubiquitin molecule can become linked to the terminal carboxylate of another. This ubiquitinylated protein is degraded by an ATP-powered 26S protease complex and ubiquitin is then recycled; approximately 87% of the polypeptide chain is involved in hydrogen-bonded secondary structure. Prominent secondary structural features include three and one-half turns of alpha-helix, a short piece of 3(10)-helix, a mixed beta-sheet that contains five strands, and seven reverse turns. There is a marked hydrophobic core formed between the beta-sheet and alpha-helix. The molecule features a number of unusual secondary structural features, including a parallel G1 beta-bulge, two reverse Asx turns, and a symmetrical hydrogen-bonding region that involves the two helices and two of the reverse turns. ( From J Mol Biol 194: 531-44 (1987) )

Chapter 14. Examples of Homonuclear 2D Experiments

Documents

2D homonuclear correlation 1H solid-state NMR by wPMLG

MULTI-DIMENSIONAL EXPERIMENTS€¦ · NMR TRAINING MULTI-DIMENSIONAL EXPERIMENTS What to Cover ‣ Introducing a second dimension ‣ COSY, NOESY, TOCSY, HSQC, HMBC ‣ 2D Processing

Relativistic effects on homonuclear triel clusters · Relativistic effects on homonuclear triel clusters ... on the crystal chemistry and the properties of compounds containing

1D and 2D Experiments Step-by-Step Tutorial

Journal Name RSCPublishing · The extension of 2D-IR spectroscopy to excited states via so-called transient or non-equilibrium 2D-IR spectroscopy (T-2D-IR) experiments was first reported

7- Double Resonance 1. Types of double resonance experiments 2. 1 H-{ 1 H} Homonuclear Decoupling 3. 13 C-{ 1 H} Heteronuclear Decoupling

The MOs of a Homonuclear Diatomic Molecule · The MOs of a Homonuclear Diatomic Molecule Calculations Consider a homonuclear diatomic molecule A 2, whose two atoms A are identical

8.9 Two Dimensional NMR COSY - UW-Madison … Two Dimensional NMR The most common types of 2D experiments are listed below. COSY: Homonuclear correlated spectroscopy.Correlation between

1D and 2D Experiments Step-by-Step Tutorial - Advanced ... · 5/16/2006 · 2-D Inverse 2 Experiments 2 2D edited HSQC 2.1 Sample: 20 mg Brucine in CDCl3 Preparation experiment 2.1.1

Basic 1D and 2D Experiments - University of …nmrlab.chem.upenn.edu/s/basic_1d_and_2d...Basic 1D and 2D Experiments Introduction to 1- and 2-dimensional NMR Spectroscopy 001 The information

Multidimensional NMR Experiments - Organic Synthesis … · 02/12/2014 · Multidimensional NMR Experiments ... synthesis of manzamine. New Developments ... Caryophyllene Oxide. 2D

Homonuclear & Heteronuclear bonds

MO diagram for homonuclear diatomic molecules Li 2 through N 2 MO diagram for homonuclear diatomic molecules O 2 and F 2

Homonuclear 2DJ spectroscopy - HOMO2DJ

Basic 1D and 2D Experiments - nmr.chem.ucsb.edu...inverse 2D experiments. The description of the experiments covers the basic principle, the information content and interpretation

2D-LDA - Face Recognition on ORL Dataset · Intro to 2D-LDA Experiments Conclusion Methodology Results Experiment Design In each test, I compare the performance of 2D-LDA vs 2D-PCA

Homonuclear 2DJ spectroscopy - HOMO2DJ All 2D experiments we’ve analyzed so far are used to find out correlations or connections between spin systems

8.9 Two Dimensional NMR COSY - UW-Madison …8.9 Two Dimensional NMR The most common types of 2D experiments are listed below. COSY: Homonuclear correlated spectroscopy.Correlation

Homonuclear & Heteronuclear bonds Homonuclear bondsHetronuclear bonds Ethane (C 2 H 6 ) Hydrazine (N 2 H 4 ) Hydrogen peroxide (H 2 O 2 )

Basic 1D and 2D Experiments - College of Arts and Sciences · Basic 1D and 2D Experiments Introduction to 1- and 2- ... _____ 18 1.5.3 Tuning and Matching 13C (non ... 2.1 General