水素−重水素交換質量分析法（HDX-MS）を用いた 蛋白質凝集体 … ·...

212

2019年3月8日　受理* 豊田理研スカラー 京都大学大学院理学研究科生物科学専攻

水素─重水素交換質量分析法（HDX-MS）を用いた蛋白質凝集体解析法の開発

水素−重水素交換質量分析法（HDX-MS）を用いた

蛋白質凝集体解析法の開発

Elucidation of Protein Aggregation Using Hydrogen-Deuterium Exchange mass (HDX-MS)

Naotaka SEKIYAMA

Cells are exposed to environmental stresses and thus possess the stress responses to maintain their

homeostasis. One of those is a formation of stress granules. Stress granule is an aggregation of protein and mRNA that is formed when cells sense stress. Intrinsically disordered proteins (IDPs) mediate the assembly of stress granules. In this study, we aimed to examine the self-assembly mechanism of the IDPs using structural biology methods such as fluorescence polarization assay or HDX-MS (Hydrogen-Deuterium eXchange mass).

１．研究概要

２．実験

水素─重水素交換質量分析法（HDX-MS）を用いた 蛋白質凝集体解析法の開発

*関　山　直　孝*

Elucidation of Protein Aggregation Using Hydrogen-Deuterium Exchange Mass (HDX-MS)

*Naotaka SEKIYAMA*

Cells are exposed to environmental stresses and thus possess the stress responses to maintain their homeostasis. One of those is a formation of stress granules. Stress granule is an aggregation of protein and mRNA that is formed when cells sense stress. Intrinsically disordered proteins (IDPs) mediate the assembly of stress granules. In this study, we aimed to examine the self-assembly mechanism of the IDPs using structural biology methods such as fluorescence polarization assay or HDX-MS (Hydrogen-Deuterium eXchange mass).

213水素─重水素交換質量分析法（HDX-MS）を用いた蛋白質凝集体解析法の開発

３．結果

４．結論

REFERENCES

図1

図2