212 2019 年3 月8 日受理 * 豊田理研スカラー京都大学大学院理学研究科生物科学専攻水素─重水素交換質量分析法（HDX-MS）を用いた蛋白質凝集体解析法の開発１．研究概要ｵｽ¡3|"ガmfi~ŁÁ|Rs†}lflcvÖä~?3>¶Ü¦y“|]e‘ダ;_¥¶H¦dx~Å¦|Ł Áピｱnh‒dŁÁピｱ’¡cfŁÁ=ｯQ|7Cs†‒ﾃ|ﾙ’ C?4 ~èヅÖgh‒dｵｽnn¼Æ ‹Õゲｴ‘\~ŁÁ¶A｣u‒’c‰æÀ¯Ägﾘ¶Fk‒y“c#p~ SC?4 ~ｼﾑnFïs†‒dｼﾑnÞat y SC?4 |¡cŁÁmfi SC?4 ¶Üﾗu‒y“q,~ C?4 ｷûﾃ|ﾙnｷûu‒du‒’cr†fi C?4 ｷûﾃ|ﾙü n｡Î×wtヅｫu‒r’cﾃ|ﾙbC?4 ベ`èヅÖn7Cs†‒dr†nŁÁピｱh‒dｵｽä7Cs†‒ ŁÁピｱ~｠9¡L `S |ヮtcäガ|¡ł…Ä-ÃヴÔ(‘\~ SC?4 n÷flﾛƒ†ｼﾑnÅQ}|Þat }i‒ncｵｽnŁÁmfiŠ<u‒’ﾞ‹m|g&tｼﾑnåズs†‒dr~ŁÁピｱ~7C¡cｵｽ~ŁÁ ケ=_¥’t}ゴﾊ‘8ð¶G{}i‒|«ゾ·fivcx~7Cヱｩ|ゾu‒¥ﾟ+}｣ﾋ¡⁄’•\:fi†}i‘m {yd ﾜ4cr~èヅ~ブõñ’t}f‾¶ヅ“}i‒~nc%m!>ﾃ|ﾙペ;7A2 ;TZXPTYPJIRR^ 7PYUXKLXLK AXUZLPTボ ~ﾀ2ｶｻöh‒d;7A ’¡c* `¥ﾟ‘\q,~ｭÖ¥ﾟ¶Hy‘i„³ıゲビš~r’cŁÁピｱ|/u ‒ C?4 ｷûﾃ|ﾙ|#pýƒ†}i‒d*()- 4パc;7A ¶ý¤ C?4 ｷûﾃ|ﾙnch‒i6‹khVÓ|li}cdl~ ›j‘｡¶7Cu‒r’n{ﾋs†yペ==AD2 =PW[PK%=PW[PK AOIYL DLVIXIZPUTボdr~ ==AD ¡cŁÁピｱzq‘ pﾚçﾕｰ‹Â²ÃõB‘\]e‘ｵｽ_ｾ|ゾÉt}i‒r’nｦÿs†}lflcÊy}‘±ÆŁŁ-Æ’‘{ }i‒ ) dxrU､ｬ¡c%m!>ビšn7Cu‒ﾃ|ﾙèヅÖh‒ ==AD ‹„³Â»Øｺｸ‘\~¥ﾟÖ¶¥ﾟ vp+}|ﾍPu‒r’¶‾Ityd ２．実験 U､ｬ¡c%m!>ビš¶H¦ C?4 ｷûﾃ|ﾙゼ|àp｡Î×w¶ccｴbゴcｴÏJﾙザëYeペ:7H%>Dボ‹úｪ ëáe¶wi}Pfim|u‒r’¶‾}’tyd:7H%>D ’¡cゴc|kﾍtyﾃ|ﾙ¡cÌジ„³Øcｴnゴcｴ’ ÏJu‒r’¶îwtck'|ドêt}i‒„³ıゲb™¶ü,u‒Eeh‒d:7H%>D ¡c„³Â»Øｺｸ¢~=w Ún5¦mžþs†}i‒r’mfi * cC?4 ｷûﾃ|ﾙn7Cu‒ ==AD ‹œØÂÀ|li}«Sôh‒ùｾ>¡ベid j,¸Ã¬À¡c$ｿﾂ{tｳ¶wi}ﾕﾉty¾ÃßŒÃŁﾃ|ﾙ¶wiyd¾ÃßŒÃŁﾃ|ﾙ~>ﾙ¶･ﾔ u‒y“cﾄáÝáﾍge|›‒ﾒÛ¶ﾆ{ydﾄáëáﾎ¡ C8%-+((ペDOPSIK_[ボ|ÝáX¶ﾈ｢tÙwtyd=6%>D ¡c=6 ½µ’t} 4JJ[JUXL CA%>DペEOLXSU 8PYOLX DJPLTZPMPJボ¶wicｨõ｡’t} (&)!¯ゲckh’ (&)!¯ゲ ýS¹ıÄÀ¶Ùwtyd>D ¡ =6B 8RLLZペEOLXSU 8PYOLX DJPLTZPMPJボ¶Ùwtyd 水素─重水素交換質量分析法（HDX-MS）を用いた蛋白質凝集体解析法の開発 * 関　山　直　孝 * Elucidation of Protein Aggregation Using Hydrogen-Deuterium Exchange Mass (HDX-MS) * Naotaka SEKIYAMA * Cells are exposed to environmental stresses and thus possess the stress responses to maintain their homeostasis. One of those is a formation of stress granules. Stress granule is an aggregation of protein and mRNA that is formed when cells sense stress. Intrinsically disordered proteins (IDPs) mediate the assembly of stress granules. In this study, we aimed to examine the self-assembly mechanism of the IDPs using structural biology methods such as fluorescence polarization assay or HDX-MS (Hydrogen-Deuterium eXchange mass).

水素−重水素交換質量分析法（HDX-MS）を用いた蛋白質凝集体 … · 水素─重水素交換質量分析法（HDX-MS）を用いた蛋白質凝集体解析法の開発

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Page 1: 水素−重水素交換質量分析法（HDX-MS）を用いた蛋白質凝集体 … · 水素─重水素交換質量分析法（HDX-MS）を用いた蛋白質凝集体解析法の開発

212

2019年3月8日　受理* 豊田理研スカラー京都大学大学院理学研究科生物科学専攻

水素─重水素交換質量分析法（HDX-MS）を用いた蛋白質凝集体解析法の開発

水素−重水素交換質量分析法（HDX-MS）を用いた

蛋白質凝集体解析法の開発

Elucidation of Protein Aggregation Using Hydrogen-Deuterium Exchange mass (HDX-MS)

Naotaka SEKIYAMA

Cells are exposed to environmental stresses and thus possess the stress responses to maintain their

homeostasis. One of those is a formation of stress granules. Stress granule is an aggregation of protein and mRNA that is formed when cells sense stress. Intrinsically disordered proteins (IDPs) mediate the assembly of stress granules. In this study, we aimed to examine the self-assembly mechanism of the IDPs using structural biology methods such as fluorescence polarization assay or HDX-MS (Hydrogen-Deuterium eXchange mass).

１．研究概要

２．実験

水素─重水素交換質量分析法（HDX-MS）を用いた蛋白質凝集体解析法の開発

*関　山　直　孝*

Elucidation of Protein Aggregation Using Hydrogen-Deuterium Exchange Mass (HDX-MS)

*Naotaka SEKIYAMA*

Cells are exposed to environmental stresses and thus possess the stress responses to maintain their homeostasis. One of those is a formation of stress granules. Stress granule is an aggregation of protein and mRNA that is formed when cells sense stress. Intrinsically disordered proteins (IDPs) mediate the assembly of stress granules. In this study, we aimed to examine the self-assembly mechanism of the IDPs using structural biology methods such as fluorescence polarization assay or HDX-MS (Hydrogen-Deuterium eXchange mass).