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Biomolecular vibrational spectroscopy
Methods in Molecular Biophysics, Spring 2009
Basics of normal mode theoryAmide vibrations in proteins
Nuclear resonance vibrational spectroscopyTwo-dimensional vibrational spectroscopy
Small molecule vibrational spectoscopy
Amide vibrational spectra in proteins
Deconvoluting complex spectra
Deconvoluting complex spectra
Fitting conformational preferences
Fitting conformational preferences
Complementary vibrational probes
cf. Guo, et al. Inorg. Chem. 47:3969, 2008
Nuclear resonance vibrational spectroscopy
“vibrationalMossbauer”
only 57Fecontributes (nobackground)
intensity isproportion to KEin mode due toiron
“acoustic”librational modesvisible (inprinciple)
Model compound IR spectra
Model compound Raman spectra
Model compound NRVS spectra
Moving to a ferrdoxin protein
Isotope shifts in the protein environment
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