Analog to Digital Microfluidic Converter - COMSOL Multiphysics · Analog to Digital Microfluidic...

Analog to DigitalAnalog to DigitalMicrofluidic Converter

Renaud Dufour Chang Wu Farida Bendriaa Vincent Thomy and Vincent SenezRenaud Dufour, Chang Wu, Farida Bendriaa, Vincent Thomy and Vincent SenezBioMEMS Group, IEMN (UMR 8520 – University of Lille Nord de France)

Presented at the COMSOL Conference 2009 Milan

Introduction

Nowadays, 2 kinds of microfluidic technologies:

Continuous/Analog systems (micro-channels).

Digital systems(droplets displacement)

→ EWOD

Introduction

European Nanobe project :

Objective: Real time control of bioprocesses.

BioMEMS Group: Samples preparation module using a DigitalBioMEMS Group: Samples preparation-module using a Digital microfluidic technique.

Need to develop Analog ↔ Digital interfaces.p g g

Table of contents

I. Modeling and Calibration1 C ill filli i l ti1. Capillary filling simulations 2. Passive valves

II. ADMC device1 d i1. design2. results

Conclusion

Modeling and calibration

Dynamic capillary filling using two-phase flow, phase field method

Theoretical models

Washburn

Results :

Zeng

Modeling and calibration

Passive valves

3D simulation with phase field method

Constant inlet flow rate (100µL/min)

Hydrophobic channels(theta = 110°)(theta 110 )

200µm thickness

Modeling and calibration

Pressure barrier according to valve width W2 (for constant channel width W1)

10

12

14

mba

r)

Theoretical model

4

6

8Pr

essu

re b

arrie

r (m Numeric results

Analytical solution :

0

2

20 40 60 80 100 120 140 150 160 180 200 220 240 250

W2 (µm)

ADMC : Design

Air inletV1 < V2 < V3

Top view (Thickness = 200µm)

ADMC : modeling parameters

Two phase flow, phase-field application mode3D Model

Boundaries

•Wetted wallsComputation

•Inlet : laminar inflow with constant pressure

L_entr = 10cm

•30.000 mesh elements

•500.000 DOFs

P_entr = 1bar

•Outlet: atmospheric pressure

•100 hours computation time on a Sun workstation

ADMC : results

Evacuation CavityEvacuationchannel

Cavity

ADMC : results

80% of the continuous flow converted into droplets

Conclusion

ADMC device advantages:

Allowing integration of both Analog and Digital microfluidic on the same chipDelivering of constant-volume dropletsResistance to pressure variation (evacuation of liquid excess)Resistance to pressure variation (evacuation of liquid excess)Easy to integrate in an EWOD fabrication process

Further work:

Geometry and network optimizations to reduce lossesDevice manufacturing and testing

Thank you

Questions & Answers

Electrowetting on dielectric

ITO C t

Camera

Top view Lateral view

CytopSU8Nickel

Glass wafer

ANNEXE 2

EWOD – Fabrication process

ANNEXE 3: NANOBE

Nanobe Project