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5 µm
Millions of copies of a specificoligonucleotide probe
>5 760,000 differentcomplementary probes
~260 000 targets
Single stranded, labeled ‘target’
Oligonucleotide ‘probe’* *** *
1.28cm
GeneChip Probe Array
Hybridized Probe Cell
1.28cm
Probe Construction
GeneChip® Expression Analysis Procedures for Target Preparation
cDNAFragment(heat, Mg2+)
B B B B
Wash & StainScan Hybridize
(16 hours)
Biotin-labeled transcripts
RNA
AAAA
IVT
(Biotin-UTPBiotin-CTP)
Fragmented cRNA
B B
B
B
Cells
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