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partition equilibrium partition coefficient
Chromatography
Tswett : calcium carbonate, CaCO3 petroleum ether chlorophyll, carotene stationary phase mobile phase normal phase chromatography reversed-phase chromatography
normal phase chromatography CaCO3 silica alumina paper
reversed phase chromatography -C18H37 C18 octadecyl
high performance liquid chromatography (HPLC)
PHENOL-0.3% NH3
BUTANOL- ACETIC ACID
“In Fig. 2 is shown a paper chromatogram run in n-butanol-acetic acid-water mixture followed by water-saturated phenol, and spraying with ninhydrin.”
Martin : 1952 Nobel chemistry prize partition chromatography
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