partition equilibrium partition coefficient

Chromatography

Tswett : calcium carbonate, CaCO3 petroleum ether chlorophyll, carotene stationary phase mobile phase normal phase chromatography reversed-phase chromatography

normal phase chromatography CaCO3 silica alumina paper

reversed phase chromatography -C18H37 C18 octadecyl

high performance liquid chromatography (HPLC)

PHENOL-0.3% NH3

BUTANOL- ACETIC ACID

“In Fig. 2 is shown a paper chromatogram run in n-butanol-acetic acid-water mixture followed by water-saturated phenol, and spraying with ninhydrin.”

Martin : 1952 Nobel chemistry prize partition chromatography