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Here is a summary by Andy Maloney and myself. This describes what we basically do in the lab and why we have so much fun doing it. This presentation was a blast to make and I look forward to doing many more. Credit: The song is "Missing You" by Trash80 from the album Icarus. It can be downloaded from 8bitpeoples.com.
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A Tale of Two Labs
Unzipping DNAand
Molecular Motors
Microtubules
Kinesin
Slide preparation
Osmolytes
PART 2DNA Unzipping
Unzipping DNA
Components:• Unzipping construct• Optical Tweezers• DNA to unzip• Optional: proteins
We can unzip anythingFor plot below:• Red Line – protein bound to DNA• Black Line – naked DNA• Black Dotted Line- predictions of
protein locations• Arrows – missing protein
locations
F
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Unzipping can detect:• Naked DNA “sequence”• Changes to DNA sequence• proteins bound to DNA• And other possible
structures
Shotgun DNA Mapping
• Algorithm matches unzipped random digestion fragments to library of simulated unzipping fragments
• Initial data is based on yeast genome
Library of Simulated Curves
Experimental Force
Correct Match
