DNA

DNAIt’s gonna getchoo.

The basics

Space-filling Model

Super-simplified

DNA in the cell

Transcription

Transcription

Translation

Or you can watch it . . .

Junk DNA?

Junk DNA?

Tandem Repeats

A-T-T-C-G-A-T-T-C-G-A-T-T-C-G

Tandem Repeats

RFLP

RFLP

RFLP

Gel Electrophoresis

PP doesn’t like flash . . .

Gel Electrophoresis Animation

A run gel.

DNA RFLP Comparison

BUT . . .

BUT . . .

•Downsides of RFLP:

BUT . . .

•Downsides of RFLP:•Large DNA fragments

needed

BUT . . .

•Downsides of RFLP:•Large DNA fragments

needed•Large original samples

needed

BUT . . .

•Downsides of RFLP:•Large DNA fragments

needed•Large original samples

needed•Fresh samples a must

BUT . . .

•Downsides of RFLP:•Large DNA fragments needed•Large original samples

needed•Fresh samples a must•Testing is lengthy (3wks – 3

months)

Short Tandem Repeats

Short Tandem Repeats

We are a type of tandem repeat . . .

Short Tandem Repeats

We are a type of tandem repeat . . .

BUT TINY!

The 13 CODIS STR’s and

their Identity ProbabilitiesSTR African American U.S. Caucasian

D3S1358 0.094 0.075

vWA 0.063 0.062

FGA 0.033 0.036

TH01 0.109 0.081

TPOX 0.090 0.195

CSF1PO 0.081 0.112

D5S818 0.112 0.158

D13S317 0.136 0.085

D7S820 0.080 0.065

D8S1179 0.082 0.067

D21S11 0.034 0.039

D18S51 0.029 0.028

D16S539 0.070 0.089

Fortunately!